The RP-HPLC methodology was used to produce a simple, precise, and accurate method for estimating obeticholic acid. Successful elution of the drug was achieved using STD BDS C18 (150mm*4.6mm 5µm) column with 0.01N KH2PO4: acetonitrile in a 70:30 % v/v ratio as mobile phase. The ultraviolet detection was monitored at a wavelength of 215 nm at flow rate 1.0 mL/min. The validation of proposed method was carried for linearity, precision, accuracy, limit of detection, limit of quantification and robustness were determined in accordance with ICH guidelines. The system suitability parameters were investigated by injecting the standard six times, with the results falling into the acceptance standards. Between 25 and 150 percent levels, a linearity study was done, and the R2 value was found to be 0.999. Precision for repeatability was determined to be 0.8% and 0.7% for moderate precision. The LOD and LOQ values were 0.15 µg/ml and 0.44 µg/ml, respectively. The marketed formulation was assayed using the aforesaid method, and 99.50 % of it was present. Obeticholic acid degradation investigations were performed under all situations, the purity threshold was more than the purity angle and within the suitable range. The developed HPLC method provides short analysis time, high reproducibility and high sensitivity. Key Words: Obeticholic acid, RP-HPLC, Buffer, Validation