Abstract
The present work was aimed with the objective of developing simple, precise and sensitive high performance liquid chromatographic method for simultaneous estimation of quetiapine fumarate and curcumin and to execute the developed method in the simultaneous estimation of both the drugs in nanostructured lipid carrier formulations. The method was carried out using various solvents, buffers, at different flow rates for the complete separation and estimation of both the drugs. Using ICH guidelines, the developed method was validated. After various trials the chromatographic conditions were optimized. The complete chromatographic separation and estimation of both drugs was possible using phenomenex kinetex XB-C18 100 A analytical column (3.5 μm, 4.6 mm × 150 mm) and methanol: water 70:30 v/v as a mobile phase, at the flow rate of 1.0 mL/min. Sample of 200 μL was injected and chromatogram was recorded at 290 nm in triplicates. The absorption maximum for quetiapine fumarate was found to be in 290 nm, whereas curcumin possess absorption maxima at 421 nm. The simultaneous estimation of both drugs is possible when estimated at particular single wave length. The peak response for quetiapine fumarate and curcumin was maximum at 290 nm which has been fixed for the analysis.
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