Ascosporogenesis in Ascobolus stictoideus is studied and compared with that found in other members of Pezizales. Early phases of ascus development involving spore delimiting membranes from the plasmalemma and spore formation within the epiplasm are much like those of other species of Pezizales, with the exception of the appearance of organelles, such as pigmented bodies and net bodies, which are not found in other taxa. Primary spore walls are very thick and electron translucent. Opaque globular bodies, amorphous dense material derived from net bodies, and dense membrane‐bound bodies associated with the endoplasmic reticulum appear in the epiplasm and later play a role in spore wall formation. Dense granular bodies containing small particles are transported from the epiplasm into the perisporic sac along with pigmented bodies. These deeply opaque bodies increase in size, appear to coalesce, and are deposited onto the epispore layer of the spore. The phylogenetic significance of spore wall development is discussed in light of recent molecular data.