Abstract
We describe the characterization of a gene,Pad-1,fromNeurospora crassawhich displays sequence characteristics of the RS class of hnRNA-binding proteins (hnRNP) and mRNA splicing factors. This is the first report of the isolation of a putative hnRNP gene fromN. crassa.PAD-1 showed 30% identity and 57% similarity to a protein, HCC1, which was isolated using autoantibodies from patients suffering from hepatocellular carcinoma. Both HCC1 and PAD-1 show amino acid sequence similarities to the human splicing factor, U2AF65. Mutations induced inPad-1by repeat-induced point (RIP) mutation show dominant effects on ascus and ascospore formation, a novel phenotypic class of RIP mutants. A mutant isolated from thePad-1RIP cross displayed a severe vegetative growth defect and dominant effects on ascus development, indicating thatPad-1is essential for both asexual and sexual development.
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