Determination Of Total Phenolic Content Research Articles

The Effect of Three Variants of Extracting Solvents on the Total Phenolic Content and Antioxidant Activity of Diospyros Blancoi Seeds

ABSTRACT In this study, the effects of methanol, acetone, and ethyl acetate solvents on total phenolic content and antioxidant activity in Diospyros blancoi seeds extract were examined. Maceration extraction technique was selected in extracting the seeds. Determination of total phenolic content (TPC) and antioxidant activity used Folin-Ciocalteu and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, respectively. Radical scavenging activity data were interpreted from the calculations of the half maximal inhibitory concentration (IC50) values. TPC was found to be higher (603.43 ± 0.75 mg gallic acid equivalent/g dry powder) in D. blancoi seeds in the acetone extract compared to the methanol and ethyl acetate extracts. The highest antioxidant activity for D. blancoi seeds extracts was in methanol solvent (IC50: 2.72 μg/mL), with IC50 of ascorbic acid as a positive control was 3.34 μg/mL. These results indicate that TPC contributes to antioxidant activity in D. blancoi seeds.

Determination of Total Phenolic Content, Antioxidant and Monophenolase Inhibition Activities of Carica papaya Peel Extracts in Solvents with Different Polarity

Dysregulation of melanin production that is catalyzed by an enzyme known as tyrosinase may lead to skin hyperpigmentation. Even though fruit peels are often regarded as waste product, they can be a good source of phytochemicals with potent biological activities that can be utilized to inhibit tyrosinase and reduce hyperpigmentation. This study was carried out to determine the phenolic content, antioxidant and monophenolase inhibition activities of Carica papaya (C. papaya) peel extracts prepared using three solvents with different polarity; ethanol, ethyl acetate and petroleum ether. The total phenolic content was determined according to Folin-Ciocalteu method while the antioxidant and monophenolase inhibition activities of the plant extracts were evaluated using 2,2- diphenyl-1-picrylhydrazyl (DPPH) and tyrosinase inhibitory assays respectively. There was no significant difference in total phenolic content and antioxidant activities of the extracts (p > 0.05). However, the ethanolic extract of C. papaya peel showed the highest phenolic content (0.58 ± 0.19 mg/g) and maximum antioxidant activity (IC50 = 164.27 μg/mL) compared to ethyl acetate and petroleum ether. There was a significance difference in monophenolase inhibition activities of the extracts (p < 0.05). Ethyl acetate extract exhibited excellent monophenolase inhibition activity (82.69 ± 2.57 %) compared to other solvents. Finding of this study can be used for future researches to study the biological activities and elucidate the chemical structure of active compounds involved.

Determination of Total Phenolic and Flavonoid Contents of Some Medicinal Plants Growing in Çaldıran Province of Van

Çalışmanın amacı, Çaldıran/Van’da yetişen ve halk arasında sıkça tüketilen bazı tıbbi bitkilerin, toplam fenolik (TFM) ve flavonoid (TF) miktarlarının belirlenmesi ve farklı organlardan elde edilen su ekstrelerindeki olası farkların ortaya konmasıdır. Alcea pallida (Hiro) (Willd.) Waldst. &. Kit., Rumex scutatus L. (Evelik), Mentha longifolia (L.) L. (Dağ Reyhanı), Erigeron acris L., Achillea vermicularis Trin. (Civan Perçemi) bitkileri Van/Çaldıran'dan toplanmıştır. Ekstrelerin TFM ve TF içerikleri spektrofotometrik olarak ölçülmüştür. En yüksek TFM ve TF madde E. acris bitkisinin tohum ekstresinden elde edilmiştir. Aynı bitkinin gövde ekstresi ve M. longifolia tohum ve gövde ekstreleri de yüksek fenolik ve flavonoid madde taşıyan bitki kısımları olmuştur. Sonuçta, kullanılan beş farklı bitkiden alınan çiçek, gövde ve tohum ekstrelerinin hepsinde çiçek ve/veya tohum ekstrelerinin gövde ekstrelerine göre daha verimli olduğu ortaya konmuştur. E. acris ve M. longifolia bitkilerinden elde edilen sonuçlar, bu bitkilerin tıbbi amaçlara uygun olarak kullanılabileceğini, hem tohum hem gövde kısımlarının yoğun fenolik ve flavonoid içerdiğini göstermiştir.

Diagnose the Bioactive Compounds in Flaxseed Extract and Its Oil and Use Their Mixture as an Antioxidant

The aim of this study is to extract the important antioxidants components of flaxseed in different ways. The Determination of total phenol content, antioxidant activity and percentage of linoleic acid inhibition by DPPH for the components (Lignan, oil, 80 and 100% ethanolic extract and the deionized water extract). The components of flaxseed oil were identified using GC/MS. The efficiency of the mixture (80% ethanolic extract and oil) was determined by inhibiting the process of oxidation of linoleic acid during storage periods (0, 7, 14 and 21 days) at laboratory temperature, and the following results were obtained: The yield of lignan, oil, ethanol extract (80 and 100)% and deionized water were (0.12,40.52,10.9,9.6 and 13 )g.100g-1 seed respectively, while the total phenolic content of the above components was (700,1165,3315,2098 and 483)mgGAE.100g-1,respectively. Flaxseed oil gave the highest antioxidant activity 79.3% with an inhibition percentage 73.19%, compared with ethanolic extract, lignan and water extract. The diagnosis of GC/MS flaxseed oil showed that the oil was contained in compounds with antioxidant activity, including mono, di and tri-terpens such as Copaene, Monoterpene, ?- Sesquiphellandrene, Squalene, diethyl phthalate ,?-Sitosterol and Hexadecanoic acid, as well as, the presence of ascorbic acid and gamma tocopherol with good concentrations (8.68 and 2.62)% respectively. The mixture of 80% ethanol and oil extract showed an effect in decreasing of peroxide values with an increase of concentration of the added mixture (0.1, 0.4 and 0.10 mg.mg-1). The maximum reduction of the peroxide value at the concentration was 0.6 mg. mg-1, compared with the standard sample during the different durations of storage.

Screening for antioxidant activity of vegetable and fruit by-products and evaluating the ability of coffee sediment to preserve fish meal

In this study, we collected and prepared ethanol extracts from 30 vegetable and fruit by-products and screened for antioxidant activities using three methods, including determination of total phenolic content (TPC), 2,2-diphenyl-1-picrylhydrazyl free radical scavenging assay (DPPH assay), and ferric reducing/antioxidant power assay (FRAP assay). The coffee sediment (TN-16) and ginger peel extract (TN-17) showed vigorous activities and higher than those of other samples (p < 0.05). The TPC in TN-16 and TN-17 were 66.14 mgGAE/g and 146.52 mgGAE/g, respectively. For the DPPH assay, the IC50 inhibited the values of 16.73 µg/mL with TN-17 and 33.57 µg/mL with TN-16. For the FRAP, a significant difference in absorbance was observed between all samples (p < 0.05), and TN-16 and TN-17 have strong activities with higher absorbances. Besides, fifteen compounds may present in TN-16 namely, metiamide, manitol, 3-amino phenol, malicyamide, phenyl ethanolamine, 3-methoxyamphetamine, caffeine, nisoldipine, doxenitoin, dicyclohexyl phthalate, febuprol, 2.4 xylidine, glycerol 2-palmitate, 4-(benzylamino) phenol, o-toluidine were identified by HPLC-EIS-MS. In addition, the coffee sediment extract was investigated the ability to preserve fish meal in 30 days at the room temperature. The result showed that the coffee sediment was the potent extract for the protection of fish meal against lipid peroxidation as compared to synthetic antioxidants.

In vitro antioxidant analysis and quantitative determination of phenolic and flavonoid contents of Emilia sonchifolia (L) D.C (Asteraceae) leaf extract and fractions

Emilia sonchifolia (L.) D.C. is a medicinal plant from the family Asteraceae known for a wide range of ethnomedicinal uses such as management of inflammatory diseases, pains, cancer, diabetes, cataract, asthma and liver disease. This research was aimed at assessing the antioxidant potential and quantitative determination of phenolic and flavonoid contents of the extract and fractions of Emilia sonchifolia leaves. The leaves of the plant were processed, extracted and partitioned successively and exhaustively with dichloromethane (DCM) and ethyl acetate. Phytochemical screening was carried out on the methanol extract using standard methods. Antioxidant evaluation of the methanol extract and fractions of Emilia sonchifolia leaves was carried out using Ferric Reducing Antioxidant Power (FRAP) assay and 2, 2, diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. Quantitative determination of total phenolic content and total flavonoid content was done. The extraction and partitioning yielded the crude extract and respective fractions. The phytochemical screening revealed the presence of tannins, saponins, flavonoids, alkaloids and cardiac glycosides. Antioxidant evaluation of the crude extract and fractions of the plant exhibited significant (p<0.001) antioxidant activity in both assay though not comparable with the standard agent, ascorbic acid. In DPPH assay, the highest percentage inhibition was observed in ethyl acetate fraction (62%) followed by the extract (61%), DCM fraction (55%) and aqueous fractions (54%) at 100 µg/mL. In FRAP assay, the highest reducing power was exhibited by ethyl acetate fraction (0.457 nm) followed by the extract (0.444 nm), DCM fraction (0.441 nm) and aqueous fraction (0.439 nm). Although these activities were significant, they were not comparable to that of the standard drug ascorbic acid (0.914 nm). In total phenolic content assay ethyl acetate fraction had the highest phenolic content (5.804 mg/g), followed by crude extract (2.500 mg/g). The total flavonoid content was observed to be highest in ethyl acetate fraction (10.556 mg/g), followed by crude extract (4.444 mg/g). From this research work it was observed that Emilia sonchifolia leaves have a good antioxidant activity which could be responsible for its wide range of ethnomedicinal activities and this lends scientific credence for the use of this plant in the management of disease conditions.

Depolymerization of Lignin from Extracted Solid Waste of Cupressus lusitanica Mill. Biomass Using Imidazole

Implementation of green chemistry and biorefinery concept are needed to boost production of biomass-derived fuels, chemicals, and materials with cost-effective processing of sustainable feedstock. The use of imidazole as a novel solvent for biomass pretreatment creates an approach that helps accomplish this concept. The present work is dedicated to study the pretreatment of residual lignocellulosic biomass, namely, extracted solid waste of Cupressus lusitanica, by application of the alkaline solvent—imidazole. The pretreatment allowed obtaining cellulose- and hemicellulose-rich fractions, whereas lignin was depolymerized. Both cellulose and hemicellulose recovery were highly dependent on the reaction conditions. The highest cellulose content was obtained at 160 °C for 4 h and was 40.7 ± 0.6 wt% with a delignification yield of 65.2 ± 0.4 wt%. The effect of biomass delignification on the efficiency of enzymatic digestibility was also analyzed and it was observed a good linear relationship between the delignification yield and the glucan to glucose yield. The presence of added-value phenolic compounds from depolymerized lignin in recovered imidazole was analyzed by capillary electrophoresis and determination of total phenolic content and antioxidant activity was also performed. These compounds were tentatively identified and their structures proposed on the basis of the HPLC–MS analyzes.

Simultaneous determination of antioxidant properties and total phenolic content of Siraitia grosvenorii by near infrared spectroscopy

This study was conducted to evaluate the feasibility of using near-infrared spectroscopy (NIRS) for simultaneous determination of total phenolic content (TPC) and antioxidant properties of Siraitia grosvenorii, specifically 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), ferric reducing antioxidant power (FRAP), and 2,2-diphenyl-1-picrylhydrazyl (DPPH). Partial least squares (PLS) coupled with wavelength selection was applied to develop NIRS calibration models based on a set of 80 samples. All models established were cross- and test set validated. The root mean square error of cross validation (RMSECV) and the coefficient of determination for cross validation $$\left( {{\text{R}}_{{{\text{cv}}}}^{2} } \right) $$ were calculated to evaluate the performance of models. The results showed good precision of models for TPC ($${\text{R}}_{{{\text{cv}}}}^{2}$$ = 0.9829, RMSECV = 0.0426), DPPH ($${\text{R}}_{{{\text{cv}}}}^{2}$$ = 0.9477, RMSECV = 0.0279), ABTS ($${\text{R}}_{{{\text{cv}}}}^{2}$$ = 0.8958, RMSECV = 0.0154), and FRAP ($${\text{R}}_{{{\text{cv}}}}^{2}$$ = 0.9137, RMSECV = 0.0176). In addition, 20 unknown samples were used to evaluate the predictive ability of the obtained models, and the ratio performance deviation (RPD) for TPC, ABTS, DPPH, and FRAP ranged from 2.70 to 4.57, which indicate that these models had acceptable predictive ability. These results demonstrated that NIRS has the potential to be a time saving and cost-effective method for the determination of both TPC and antioxidant capacities of Siraitia grosvenorii.

Ultrasound-assisted extraction of phenolic compounds from avocado leaves (Persea americana Mill. var. Drymifolia): optimization and modeling

AbstractUltrasonic-assisted extraction (UAE) was performed to extract the total phenolic compounds from avocado (Persea americana Mill. var. Drymifolia; Lauraceae) leaves with different electric powers (UAE 0%, UAE 60%, and UAE 100%) and extraction times. Ultrasonic extraction parameters were optimized by using a mathematical model made by stepwise regression (SWR) for the determination of the maximum total phenolic content (TPC) and their antioxidant activity. Moreover, TPC extraction was modeled applying heterogeneous models to elucidate the involved mechanisms phenomena that determine the extraction rates. Optimization results found that the maximum value of TPC reached 48,732 mg GAE/100 g D.M. at 84.5% electric power and 29.7 min of extraction, which was superior to 0% electric power UAE. It was also found that the ultrasound causes the degradation of phenolic compounds, whereas the final extraction yield of TPC increases and their antioxidant activity decreased with the increase of ultrasound electric power. Proposed models gave a satisfactory quality of fit data using a second-order reaction for the degradation kinetics of TPC under ultrasound application. The estimated effective diffusivity values were in a range from 1.3889 × 10−11 m2/s to 2.2128 × 10−11 m2/s for the UAE 0% and UAE 100%, respectively. UAE significantly increased the extraction yield through the enhancement of the effective diffusivity, demonstrating that it is a promising technology to extract phenolic substances from avocado leaves.

Wound Healing Activity of Ethanolic Extract of Selaginella Bryopteris on Rats

The objective of the present study was to determine wound healing activity of ethanolic extract of Selaginella bryopteris on rats. The whole plant of S. bryopteris Linn. was collected from Andhra Pradesh, India and extraction was done using ethanol. GC-MS analysis was performed to determine active metabolites present in the extract followed by determination of total phenolic and flavonoid contents. In vivo wound healing activity of ethanolic extract was evaluated using excision wound model. The extract was applied topically on animals by preparing ointment in two concentrations (5% and 10%) where soframycin (10%) was taken as positive control. Antioxidant activity of S. bryopteris extract was observed by measuring oxidative enzymatic levels i.e. Superoxide dismutase (SOD), Catalase (CAT), reduced Glutathione (GSH) and lipid peroxidation (LPO) in animal tissues. Histopathological studies of excised skin were carried out after the experimental period. The contraction rate of the wound was higher and dose-dependent in rats treated with 5% and 10 % ointment of extract in comparison to untreated control group. The drug treated groups showed recovery phase and the percentage of healing was more in 10% at the end of experimental period. Results exhibited sufficient insights on the healing process with normal recovery stages and restored oxidative enzymatic levels. Histopathological findings provided additional positive results; the dermis with proliferating capillaries and skeletal muscle were replaced by cellular fibrous tissue and collagen fibers. Overall, the results showed that ethanolic extract of S.bryopteris was an interesting traditional agent that possess significant wound healing activity.

Phytochemical screening and determination of total phenolic and total flavonoid contents of pear peel extracts

The study was carried out to investigate the presence of different phytochemicals as well as to determine the total phenolic and flavonoid contents of green pear/european pear peels and yellow pear/asian pear peels. Phytochemical screening of the pear peels was carried out following standard protocols. Total phenolic content was determined by Folin-Ciocalteu method and total flavonoid contents was determined by Aluminum Chloride Colorimetric method, measured using n-hexane, etil acetate, and methanol. Phenolics, flavonoids, tannins, alkaloids, and saponins are present in green pear and yellow pear peels. The total phenol and flavonoid contents are highest in methanol extracts in which green pear peels have a higher concentration than yellow pear peels. In conclusion, the green and yellow pear peels are rich in phenolic compounds and have great potential as natural antioxidants. Therefore, the results of this research should be considered to provide the possibility for future developments of highly functional antioxidants.

Antioxidant activity, total phenolic and flavonoid content determination of some selected medicinal plants from Kavre District of Nepal

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Isolation and Quantification of Lycopene and Determination of Β-Carotene and Total Phenolic Contents from Tomato (Lycopersicum Esculentum ) by using Various Methods

Isolation and Quantification of Lycopene and Determination of Β-Carotene and Total Phenolic Contents from Tomato (Lycopersicum Esculentum ) by using Various Methods

Determination of microbial load, total Phenolic and flavonoids contents in polyherbal formulation “yograj guggulu vati”

Yograj guggulu vati (YGV), a polyherbal formulation is recommended for the management of diseases like arthritic, anodyne or analgesic, spasm, muscle relaxant, flatulence, digestive problem, cough, hyperglycaemia, fat burner and obesity. Though Yograj guggulu vati is widely used for the treatment of diseases in Ayurvedic System of Indian Medicine, but till date, it’s Phenolic and flavonoids contents and contamination studies have not been carried. In the present article, we evaluated the total phenolic and flavonoids contents and contamination of YGV. Total phenolic contents were evaluated by Folin Ciocalteu reagent. Aluminum chloride colorimetric method was used for the determination of total flavonoid contents. Contamination study such as microbial load was also performed. Microbial load study revealed that total bacterial counts and total fungal counts were under limits. The total phenolic content and total flavonoid content were 190.16 mg/g and 20.87 mg/ g dry extract respectively. Microbial load studies showed that the formulation has a good quality and purity. Presence of abundance phenolic and flavonoids compound indicated that YGV can be used for different biological activities.&#x0D; Keywords: Microbial load, Yograj Guggulu Vati, total phenolic contents

Physicochemical properties of chili padi Centil (Capsicum frutescens) powder dried at different drying temperatures

padi Centil (Capsicum frutescens) powder with better physicochemical properties. The effects of drying at different temperatures (50˚C, 60˚C, and 70˚C) on chili padi Centil powder was evaluated through its functional properties, including moisture content, color, water holding capacity, oil holding capacity and water solubility index as well as total phenolic content determination. Chili padi Centil powder dried at 70˚C had permitted level of moisture content (10.38%). Drying treatment at 70˚C also produced significantly smaller particle size powder (1301.1821 μm) with higher water holding capacity (2.67%), oil holding capacity (1.3%) and water solubility index (7.65%) compared to chili padi Centil dried at 50˚C and 60˚C. The powder dried at 50˚C and 70˚C showed significant visible color difference where 50˚C drying temperature resulted in intense red color powder whereas 70˚C drying temperature resulted in degraded orange color powder. The drying treatment significantly degraded the total phenolic content of chili powder.

Preliminary Phyto-pharmacological Investigation of Duabanga Grandiflora (Roxb. ex DC.) Walpers

The methanolic extract of Duabanga grandiflora (Roxb. ex DC.) Walpers leaves was investigated to explore in-vitro anti-arthritic, thrombolytic and antioxidant potential and subsequent detection of preliminary phytochemicals. It is used for treating various diseases by folk practitioners and rural people. Initially, BSA protein denaturation method was employed to evaluate in-vitro anti-arthritic potency, where the extract exhibited potent anti-arthritic activity with 73.52±1.01% inhibition of BSA denaturation at a dose of 1000 μg/ml compared to the standard dichlofenac sodium (83.66±1.03% inhibition at 1000 μg/ml dose). In-vitro thrombolytic efficacy was determined using streptokinase (positive control) and water (negative control), and the average lysis of blood clot was found to be 31.12±1.09%, which is indicative towards the thrombolytic effect of the extract. Antioxidant activity was evaluated by the DPPH radical scavenging method followed by the total phenolic and flavonoid content determination. The crude methanolic extract revealed the IC50 value of 129.31μg/ml and 204.89μg/ml against DPPH and standard ascorbic acid, respectively. The total phenolic content and total flavonoid content of the plant leaves were observed to be 52.81±1.74μg/ml and 25.77±1.01μg/ml, respectively. The results indicate that the leaves of D. grandiflora possess favorable anti-arthritic activity, moderate thrombolytic and excellent antioxidant activity, which is demonstrating the presence of alkaloids, phenols, flavonoids and many other biologically important phyto-constituents that needs further exploration. Bangladesh Pharmaceutical Journal 23(1): 54-60, 2020

Antioxidant Potential and Brine Shrimp Lethality bioassay of Spilanthes acmella Flower Extract

The current research study has been carried out to explore the antioxidant activity and brine shrimp lethality bioassay of different fractions from the flower extract of Spilanthes acmella. Besides, this experiment was also assessed to find out the proximate analysis and phytochemical screening by following the perfect protocol. To fractionate by soxhletion using sequential extraction techniques powdered flower of the plant were treated with different solvents including n-hexane, chloroform, ethanol and water. For the evaluation of antioxidant activity, total antioxidant capacity determination, determination of total phenolic content and total Flvonoids contents by aluminium tricholoride method were used. In addition, ascorbic acid and gallic acid was used as a standard antioxidant compound in these studies. Concerning the proximate analysis, moisture content, total ash value, acid insoluble ash and water soluble ash value were found 8.6%, 3.76%, 3.30%, 3.20% respectively. To evaluate cytotoxicity, the brine shrimp lethality bioassay was used. For phytochemical screening different extract of those solvents were utilized that disclosed the presence of alkaloids, flavonoids, phenolic compounds, Tannins, amino acids on different fractions but the absence of reducing sugar and saponins. The results of all assay showed that all the extracts of Spilanthes acmella flower possess significant antioxidant activity. In brine shrimp lethality bioassay, ethanol extract of flower effect to brine shrimp nauplii and exhibiting highest toxicity having LC50 value 1.20 μg/ml as compared to standard dimethyl sulfoxide (LC50 1.31 µg/ml). These evaluations suggest that Spilanthes acmella flowers might be a better source of antioxidants and possess important cytotoxic effect.

Phytochemical screening and determination of total phenolic content of Dendophthoe pentandra L. leaves ethanolic extract on mango host

Phytochemical screening and determination of total phenolic content of Dendophthoe pentandra L. leaves ethanolic extract on mango host

Chemical characterization and adsorption of oil mill wastewater on Moroccan clay in order to be used in the agricultural field

Oil mill wastewater (OMW) is the main liquid discharge from oil mills, it is considered as a dangerous pollutant due to its toxic chemical compounds which are unloaded directly in the environment without any treatment. The aims of this study were to evaluate the effectiveness of OMW adsorption on clay as a good method for the elimination of toxic chemical compounds and to study the application of treated OMW as an irrigation source in agricultural field. For this, Clay was collected from the city of Agourai (Meknes region, Morocco) and characterized by X-ray diffraction, X-ray fluorescence spectrometry, BET and FTIR analysis. Moreover, the treated OMW was analyzed using UHPLC-ESI-MS and the determination of total phenolic content (TPC) was also performed. However, the application of the treated OMW in agricultural field was performed by the determination of its effect on the germination of Lepidium sativum seeds (in vitro) and as a source of irrigation of Vicia faba plants (in situ). The results of this study showed that OMW had the following physicochemical characteristics: average pH of 4.88, TPC of 4.75 g/l, COD of 80 g/l, BOD5 of 18.72 g/l, conductivity of 16.05 cm-1, dry matter of 135.7 g/l and volatile matter of 58.7 g/l. The adsorption on clay had increased the pH from 4.88 to 6.14 and reduced significantly the organic matter (42% of COD and 57.4% of phenolic compounds). UHPLC-ESI-MS analysis showed the presence of a wide variety of organic compounds in OMW, with the appearance of new compounds after adsorption. Moreover, the use of treated OMW as a source of irrigation showed a significant effect on the germination of Lepidium sativum seeds and the growth of Vicia faba plants. From this study, we can conclude that the adsorption on clay is a good method for the treatment of OMW, which became non-toxic for environment and can be used as a source of irrigation in agricultural field.

Phytochemical Investigations and In Vitro Anti-inflammatory Activity of ethanolic extract of Convolvulus pluricaulis Choisy and Evolvulus alsinoides Linn. : A comparative study on Shankhpushpi species

The objective of the study was to compare the phytochemical investigation and anti-inflammatory activity of ethanolic extract of two variety of shankhpushpi i.e. Convolvulus pluricaulis Choisy and Evolvulus alsinoides Linn. The phytochemical analysis was performed followed by characterization with FTIR and NMR spectroscopy and total phenolic content determination. Anti-inflammatory activity was evaluated by two i vitro models i.e. heat induced and hypotonic solution-induced hemolysis. Total phenolic content of C. pluricaulis and E. alsinoides was 668.143 + 1.107 and 593.453 + 0.565 micro g GAE/ml extract respectively. In heat hemolysis, inflammation was significantly reduced at 50, 100 and 300 micro g/ml in CP and at 50, 100 and 200 micro g/ml in EA. Both extracts were effective in inhibition of hemolysis and results of both were compared than CP shows better results than EA.