Determination Of Rotenone Research Articles

Rapid determination of rotenone in whole blood and urine by two-dimensional ultra performance liquid chromatography- triple quadrupole/linear ion trap mass spectrometry

Rapid determination of rotenone in whole blood and urine by two-dimensional ultra performance liquid chromatography- triple quadrupole/linear ion trap mass spectrometry

Determination of rotenone in river water utilizing packed capillary column switching liquid chromatography with UV and time-of-flight mass spectrometric detection

Fast and sensitive packed capillary column switching liquid chromatography methodology has been developed for the determination of the pesticide rotenone in river water. Sample volumes of up to 1 ml are loaded onto a 23×0.25 mm, 5 μm Kromasil C 18 packed capillary precolumn using a noneluting solvent composition of water–acetonitrile (99:1, v/v) at flow-rates up to 100 μl/min prior to solute backflushing onto a 200×0.32 mm, 3.5 μm Kromasil C 18 packed capillary analytical column using a mobile phase of water–acetonitrile (30:70, v/v) at a flow-rate of 5 μl/min. The method was evaluated using river water samples spiked with rotenone in the concentration range 0.5–50 ng/ml using UV detection. The within-assay precision was between 5.0 and 7.7% relative standard deviation (RSD, n=6) and the between assay precision was between 7.5 and 8.9% RSD ( n=6). The method was linear within the investigated mass range displaying a calibration curve correlation factor of 0.997. The mass limit of detection was 10 pg corresponding to a concentration limit of detection of 10 pg/ml, using time-of-flight mass spectrometry.

Liquid Chromatographic Determination of Rotenone in Fish, Crayfish, Mussels, and Sediments

An analytical procedure is described for determining residues of rotenone in fish muscle, fish offal, crayfish, freshwater mussels, and bottom sediments. Tissue samples were extracted with ethyl ether and extracts were cleaned up by gel permeation chromatography and silica gel chromatography. Sediment samples were extracted with methanol, acidified, partitioned into hexane, and cleaned up on a silica gel column. Rotenone residues were quantitated by liquid chromatography, using ultraviolet (295 nm) detection. Recoveries from sediment samples fortified with rotenone at 0.3 microgram/g were 80.8%, whereas recoveries from tissue samples fortified with 0.1 microgram/g ranged from 87.7 to 96.8%. Samples fortified with 0.3 microgram/g and stored at -10 degrees C for 6 months before analysis had recoveries ranging from 83.2 to 90.5%. Limits of detection were 0.025 microgram/g for sediments and 0.005 microgram/g for tissue samples.

A Case of Fatal Rotenone Poisoning in a Child

A case of a fatal rotenone poisoning in a three-and-a-half-year-old girl is described. The case report and autopsy findings are mentioned. For the extraction of rotenone out of biological samples, a solvent partitioning and silica gel open column chromatographic cleanup procedure has been used. The determination of rotenone was performed by high pressure liquid chromatography.

Simultaneous Liquid Chromatographic Determination of Rotenone and Pyrethrins in Formulations

Abstract This paper describes a reverse phase liquid chromatographic (LC) method to simultaneously determine rotenone and pyrethrins in pesticide formulations. The mixed standards along with the formulations were accurately weighed to contain approximately 200 (μg rotenone and 150 (μg pyrethrins/mL. Stabilized tetrahydrofuran was used to dissolve all substances. An aliquot was injected into the LC system equipped with a Zorbax ODS column, and chromatographed with a mobile phase of acetonitrile-water (70 + 30). Rotenone and the pyrethrins were monitored at 240 nm and 0.4 AUFS. Retention times for rotenone, pyrethrin II, and pyrethrin I were approximately 7, 11.5, and 25.5 min, respectively. For 3 different formulations analyzed 6 times each, the percent coefficients of variation were all < 3. This method is also applicable to products containing either rotenone or pyrethrins. No significant interferences were observed from the inactive ingredients of the formulations at the concentrations added.

Reverse Phase Radial Compression High Performance Liquid Chromatographic Determination of Rotenone in Formulations

Abstract This paper describes a reverse phase radial compression high performance liquid chromatographic (HPLC) method to determine rotenone in pesticide formulations. Formulations are extracted in dioxane by gentle swirling while sonicating for 5 min. A 10 μL aliquot is injected into the HPLC system equipped with a radial compression unit (Z module) containing a μBondapak C18 Radial-Pak column. The mobile phase is acetonitrile-methanol-tetrahydrofuranwater (34 + 30 + 1 + 35). Rotenone is monitored at 280 nm. Retention time for rotenone is approximately 7 min with total analysis time of 20 min. For 5 different products analyzed 11 times each, the percent coefficients of variation were all below 1.65. No interferences from 11 other rotenoids and 8 pesticides which are sometimes formulated with rotenone were observed.

High Pressure Liquid Chromatographic Determination of Rotenone and Degradation Products in Animal Chow and Tissues

An analytical procedure is described for determining residues of rotenone, rotenolone, dehydrotenone, and rotenone in admixture in animal chow and tissues. The methanol or ethyl ether extracts from samples of chow and tissues, respectively, are subjected to a liquid-liquid partitioning cleanup with hexane-acetonitrile, further cleanup on a column of silica gel, and subsequent analysis by high pressure liquid chromatography using an ultraviolet absorption detector set at 295 nm. Animal chow, mouse fetuses, and gastrointestinal tracts spiked with 0.5 ppm of each compound in admixture yielded average recoveries of 92, 51, and 79%, respectively; minimum quantities of the 4 compounds detectable in the 3 substrates averaged 0.12, 0.04, ajd 0.14 ppm, respectively. Stability studies indicate that rotenone reacts with animal chow with a half-life of 7--8 days and is photodegraded in incandescent light with a half-life of 0.65 day. No transplacental transfer of rotenone or its products was observed in fetuses from mice receiving 7 consecutive daily doses of rotenone at levels up to 25 mg/kg.

Determination of rotenone in pesticide formulations and the separation of six rotenoids by reversed-phase high-performance liquid chromatography

Determination of rotenone in pesticide formulations and the separation of six rotenoids by reversed-phase high-performance liquid chromatography

Ultraviolet and Infrared Analysis of Rotenone: Effect of Other Rotenoids

Abstract Biases evidenced by past collaborative trials of ultraviolet (UV) and infrared (IR) methods for rotenone can be caused by interference from other rotenoids that occur in extracts of cubé and derris. In the UV analysis, sumatrol, rotenolone, and deguelin cause positive errors of decreasing magnitude; elliptone, toxicarol, tephrosin, dehydrodeguelin, and dehydrorotenone produce negative biases of increasing magnitude. In the IR analysis, error depends on rotenoid concentration relative to rotenone concentration. At equal proportions, bias is positive with toxicarol and deguelin and negative with other rotenoids. The baseline version of the IR method has an inherent positive bias of approximately 8% but is superior to the base point version because of greater insensitivity to deguelin. The official first action UV method for the determination of rotenone in derris and cubé powder, 6.162–6.163, has been deleted, and the IR method, 6.164–6.165, has been revised to exclude derris products.

Separation of Rotenoids and the Determination of Rotenone in Pesticide Formulations by High-Perf ormance Liquid Chromatography

The rotenoids deguelin, B-dihydrorotenone, dehydrorotenone, rotenone, 6alpha beta, 12alpha beta-rotenolone, and tephrosin were chromatographed on 8-12 mum silica. A mobile phase of chloroform-isooctane (35+65) pumped at a flow rate of 1 ml/min through a 30 cm column was used and the absorbance of the eluate was monitored at 294 nm. Rotenone, B-dihydrorotenone, deguelin, and dehydrorotenone are completely resolved while 6alpha beta, 12alpha beta-rotenolone and tephrosin chromatograph as one peak. This method has potential as a preparative separation technique for rotenoids. Also described is a procedure to quantitatively measure rotenone in pesticide formulations. Samples were extracted with chloroform and chromatographed at a flow of 2.5 ml/min. The method is rapid (rotenone is eluted in 12 min) and reproducible.

Gas-Liquid Chromatographic Determination of Rotenone and Deguelin in Extracts of Tephrosia vogelii

Gas-Liquid Chromatographic Determination of Rotenone and Deguelin in Extracts of Tephrosia vogelii

The Determination of Rotenone in Liquid and Aerosol Formulations

The Determination of Rotenone in Liquid and Aerosol Formulations

Determination of Rotenone in Pharmaceuticals

A procedure in which chloroform was used to extract rotenone from a pharmaceutical mixture has been described. A colorimetric assay for rotenone was adapted for use as an analytical tool in product development. Both moisture and heat of reaction were shown to be detrimental in the development of the color for assay.

The determination of rotenone in rotenone-bearing plants with special reference to lonchocarpus

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Correction: Determination of Rotenone by the Use of Mercuric Acetate

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTCorrection: Determination of Rotenone by the Use of Mercuric AcetateIrwin HornsteinCite this: Anal. Chem. 1951, 23, 11, 1698Publication Date (Print):November 1, 1951Publication History Published online10 July 2002Published inissue 1 November 1951https://doi.org/10.1021/ac60059a600RIGHTS & PERMISSIONSArticle Views16Altmetric-Citations-LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (154 KB) Get e-Alerts Get e-Alerts

Determination of Rotenone as Impurity in Dihydrorotenone

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTDetermination of Rotenone as Impurity in DihydrorotenoneIrwin HornsteinCite this: Anal. Chem. 1951, 23, 9, 1330–1331Publication Date (Print):September 1, 1951Publication History Published online1 May 2002Published inissue 1 September 1951https://doi.org/10.1021/ac60057a038RIGHTS & PERMISSIONSArticle Views30Altmetric-Citations2LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (268 KB) Get e-Alerts Get e-Alerts

Determination of Rotenone by Use of Mercuric Acetate

Determination of Rotenone by Use of Mercuric Acetate

Determination of Rotenone in Small Samples

Determination of Rotenone in Small Samples

Determination of Rotenone in Derris Root

Determination of Rotenone in Derris Root

Determination of Rotenone in Derris and Cube Powders

Determination of Rotenone in Derris and Cube Powders