Determination Of Phenolic Compounds Research Articles

Development and validation of a UHPLC-ESI-MS/MS method for the simultaneous determination of organic acids and phenolic compounds in Filipendula vulgaris, Polygonum divaricatum, Hypericum linarioides, and Rheum ribes

This research focused on investigating biologically active and pharmaceutically important phytochemicals using reliable and reproducible analytical techniques specifically designed for traditional medicinal plants. Considering the complex chemical diversity present in plant extracts, reliable identification methods are crucial. The goal of this study was to develop and validate a robust UHPLC-ESI-MS/MS method for the qualitative and quantitative analysis of organic acids (OAs) and phenolic compounds (PCs). With gradient elution of water containing 0.1 % formic acid and acetonitrile containing 0.1 % formic acid as the mobile phase, OAs and PCs were separated chromatographically on a C18 reversed-phase column. Through the use of an electrospray ionization source operating in negative ionization mode, these compounds were identified on a triple quadrupole tandem mass spectrometer. The validation results clearly indicate that the method exhibited linearity with a squared correlation coefficient (R2 ≥ 0.9986), demonstrating sensitivity (LOQ: 1.08 µg/L-10.79 µg/L), precision (RSD%≤2.73), and trueness (RE% ≤ 1.53) for the OAs and PCs found in the plants. The developed UHPLC-ESI-MS/MS method was successfully applied to the qualitative and quantitative analysis of OAs and PCs in Turkish plants, which are commonly used in traditional medicine. Our study identified a total of 12 OAs and PCs in F. vulgaris, 12 in P. divaricatum, 9 in H. linarioides, and 11 in R. ribes. In particular, gallic acid had the highest content in F. vulgaris (925.67 µg/g extract) and R. ribes (1358.78 µg/g extract), while H. linarioides (730.66 µg/g extract) and P. divaricatum (4003.02 µg/g extract) had relatively high levels of quinic acid. In conclusion, the developed UHPLC-ESI-MS/MS method allows the simultaneous analysis and quantification of 2 OAs and 33 PCs in plants and proves to be a valuable tool for evaluating the efficacy of traditional medicinal plants.

An efficient determination of phenolic compounds by HPLC-DAD and their bioactivity assay from aerial parts of Eucalyptus tereticornis plant

An efficient determination of phenolic compounds by HPLC-DAD and their bioactivity assay from aerial parts of Eucalyptus tereticornis plant

Derivative Spectrophotometric Methods for Simultaneous Determination of Quercetin and Gentisic acid in Capparis spinosa L.

الشفلح هو أحدی النباتات الطبية الذي یستخدم في الطب التقليدي و یتضمن المواد الكيميائية النباتية العديدة مثل المركبات بوليفينولیة. تعتبر كلتا المركبتين، كویرستین وحامض الجینتسیك، کمركب فينولية مهمة اللتان توجدان في النباتات ولهما الخصائص الطبية الکثیرة مثل مضاد الالتهابات، مضاد الميكروبات، مضاد الأكسدة ومضاد للسرطان. لم يتم التحقق بعد من تحديد كلا المركبين معًا في مزیج ثنائي باستخدام طرق القياس الطيفي. في هذه الدراسة، تم تطوير طريقتين طيفيتين مشتقتين بسيطتين سريعتين و دقيقتين لاستخدامهما للتقدير الكمي الآنی لمادة كویرستین وحامض الجینتسیك في المزيج الثنائية وخلاصة أوراق الشفلح. الطريقة الأولی تعتمد على استخدام طریق العبور الصفري (المشتق الأول والرابع) ، بينما الطريقة الثانية هي المشتق الأول لأطياف النسبة. الخطية لمنحنيات المعايرة لكلتا الطريقتين الطيفية المشتقة يتراوح تركيزهما بين 2.0-30 ميكروغرام / مل و 4.0-80 ميكروغرام / مل لكویرستین وحامض الجینتسیك، على التوالي، في حين تراوحت نسب الاسترداد من 94.06٪ الی 105.98٪ (كویرستین) و 94.29٪ الی 113.37٪ (حمض الجینتسیك). تم استخدام الطرق المطورة بشكل فعال في التقدیر الكمي لكل من المركبات الفينولية في أوراق الشفلح..

Histidine–cysteine–copper hybrid nanoflowers as active site-inspired laccase mimics for the colorimetric detection of phenolic compounds in PDMS microfluidic devices

Although laccases are essential multicopper oxidases in biosensing and bioremediation, their widespread use is hindered by issues such as instability and high cost. In this study, drawing inspiration from the active site structure of laccase, we developed hybrid nanoflowers through the coordination of copper ions with histidine-cysteine dipeptides (HC–Cu NFs) as highly robust laccase-mimicking nanozymes. The HC–Cu NFs possessed higher laccase-like activity compared to free laccase, yielding an approximately five-fold higher Vmax with a similar Km at the same mass concentration. Additionally, HC–Cu NFs exhibited exceptional stability across diverse environments, including a broad range of pH, temperature, salinity, and extended storage periods. Notably, HC-Cu NFs catalyzed the decolorization of phenolic dyes more rapidly than natural laccase. Utilizing these beneficial features, HC–Cu NFs were incorporated in a polydimethylsiloxane-based microfluidic device, enabling the on-site colorimetric determination of phenolic compounds, such as p-aminophenol, catechol, and hydroquinone, with outstanding sensitivity. By injecting only 20 μL of the sample solution through the inlet of the device, the sample solution rapidly moves to the detection zones where the HC-Cu NFs are retained, followed by catalytic oxidation to further react with 4-aminoantipyrine to produce a colored adduct. Using the microfluidic device, the target phenolic compounds were successfully determined within 20 min at ambient conditions using an image acquired with a smartphone with the ImageJ software. This study unequivocally demonstrates that active site-mimicking dipeptide-incorporated hybrid nanoflowers have the potential to serve as nanozymes, to replace natural laccase. This lays the groundwork for the further advancement of hybrid nanoflower-type nanozymes and their applications, particularly in point-of-care testing environments.

Two-dimensional metal-organic framework nanozyme-mediated portable paper-based analytical device for dichlorophen assay

The metal-organic frameworks (MOFs) nanozyme-mediated paper-based analytical devices (PADs) have shown great potential in portable visual determination of phenolic compounds in the environment. However, most MOF nanozymes suffer from poor dispersibility and block-like structure, which often prompts deposition and results in diminished enzymatic activity, severely hindering their environmental applications. Here, we proposed colorimetric PADs for the visual detection of dichlorophen (Dcp) based on its significant inhibitory effect on the two-dimensional (2D) MOF nanozyme activity. Specifically, we synthesized a 2D Cu TCPP (Fe) (defined as 2D-CTF) MOF nanozyme exhibiting excellent dispersibility and remarkable peroxidase-like (POD-like) activity, which could catalyze the oxidation and subsequent color change of 3,3′,5,5′-tetramethylbenzidine even under neutral conditions. Notably, the POD-like activity of 2D-CTF demonstrated a unique response to Dcp because of the occupation of Fe–N4 active sites on the 2D-CTF. This property enables the use of 2D-CTF as a highly efficient catalyst to develop colorimetric PADs for naked-eye and portable detection of Dcp. We believe that the proposed colorimetric PADs offer an efficient method for Dcp assay and open fresh avenues for the advancement of colorimetric sensors for analyzing of phenolic toxic substances in real samples.

Ultrasound-assisted extraction of shikimic acid and determination of total phenolic compounds in water hyacinth: Grey-based Taguchi optimization

Shikimic acid (SA) is a precursor for formulating the antiviral drug oseltamivir phosphate (Tamiflu®). Ultrasound-assisted extraction was employed to extract shikimic acid using water as a solvent and to determine total phenolic compounds (TPC) from water hyacinth. Grey-based Taguchi optimization was used to optimize the yield of shikimic acid and TPC by varying four distinct parameters. The best results for the yield of SA, i.e., 3.3 % (20 min time, 20 mL water volume, 60 % amplitude, and 40 % pulse ratio) and 3.2 % (30 min time, 20 mL solvent volume, 40 % amplitude, and 40 % pulse ratio) were observed using single and multi-response optimization, respectively for the stem. The best results for TPC, i.e., 12.24 mg GAE/ g biomass (20 min time, 40 mL solvent volume, 40 % amplitude, and 30 % pulse ratio) and 10.53 mg GAE/ g biomass (20 min time, 20 mL solvent volume, 50 % amplitude, 40 % pulse ratio) were obtained using single and multi-response optimization, respectively for leaves. The extraction was also performed for a whole plant (without segregation), and 1.66 % yield of SA (20 min time, 20 mL solvent volume, 50 % amplitude, 40 % pulse ratio) and TPC value of 8.84 mg GAE/ g biomass (40 min time, 30 mL solvent volume, 40 % amplitude, 40 % pulse ratio) were obtained.

Separation and determination of phenolic compounds using novel deep eutectic solvent-in-water microemulsion electrokinetic chromatography

BackgroundMicroemulsion electrokinetic chromatography (MEEKC) is a mode of capillary electrophoresis with a wide range of applications in which microemulsion is utilized as background electrolyte to achieve the separation of analytes. Microemulsions are composed of oil droplets, aqueous buffer, surfactant, and co-surfactant. Currently, conventional organic reagents act as the most commonly used oil phase in microemulsions, which are unfriendly to the environment. Recently, deep eutectic solvent (DES) has become a new type of eco-friendly solvent due to its non-toxicity. Therefore, it is of great value to establish a new MEEKC method by replacing conventional organic reagents as the oil phase with DES. ResultsThe novel DES/W MEEKC method was established for phenolic compounds in Senecio scandens samples. Single-factor experiments and response surface methodology were performed to systematically optimize the crucial parameters for the method, including the type and content of the oil phase, surfactant content, concentration of borax buffer, and pH of the background solution.Under the optimized conditions, satisfactory regression curves were established for all standard analytes with correlation coefficients ≥0.9990. The method featured high sensitivity and favorable accuracy, with the instrumental detection limit in the range of 0.22–1.04 μg/mL, and intraday and interday precision for migration time expressed as relative standard deviations of 0.18–0.82% and 1.25–2.50%, respectively. The DES/W MEEKC method was successfully applied to Senecio scandens with good recoveries of 87.72–106.99%. In conclusion, the newly established DES/W MEEKC method is highly efficient, green and environmentally friendly. SignificanceDES is considered a green and efficient solvent. The DES/W MEEKC method is highly efficient and environmentally friendly. Actually, the method provides a novel and effective analytical tool for the simultaneous separation and determination of multiple phenolic compounds, especially in complex plant matrices. In the future, the DES/W MEEKC method still has the prospect of being widely used in the separation of other complex phytochemicals.

Salt-assisted liquid-liquid extraction and on-column concentration for chromatographic determination of phenolic compounds in beer

Salt-assisted liquid-liquid extraction (SALLE) and on-column concentration were evaluated to simplify the chromatographic determination of phenolic compounds in beer. Resveratrol, flavonoids (quercetin and kaempferol), and phenolic acids (caffeic, p-coumaric, and trans-ferulic acids) were used as model compounds based on their concentrations reported in craft beers. SALLE with acetonitrile was effective for sample cleanup, with recoveries ranging from 84 % to 112 %. The on-column concentration was carried out in high performance liquid chromatography by starting the gradient elution with acidified water (sample carrier stream) in a reversed-phase mode. Consequently, the analytes were retained in a narrow band, and highly polar/ionic potentially interfering species were flushed from the column. The effectiveness of the approach was demonstrated by linear correlations between the peak areas and the injected volumes from 10 to 200 µL (r ≥ 0.999), without significant variations in peak width (< 10 %), retention times (< 0.7 %), and peak symmetry (tailing factors within the range of 0.8–1.1). For all analytes, a linear response was achieved from 100 to 1000 µg L−1 (r > 0.999), with limits of detection within the range of 9–82 µg L−1. The approach was also effective for on-column concentrations of resveratrol, quercetin, and kaempferol in sequential injection chromatography (SIC), with injected volumes up to 750 µL and linear responses within 100–1000 µg L−1 (r > 0.997). The proposal reduced consumption of organic solvents (with only 770 µL required per determination in SIC) and avoided solid-phase extraction cartridges for analyte preconcentration.

Exploring the potential of phenolic and antioxidant compounds in new Rosaceae fruits.

Rosaceae fruits have been used in traditional medicine for the prevention and treatment of diseases. However, these fruits have not extensively been studied regarding their phenolic composition. Thus, this research focuses on the determination of phenolic compounds by high-performance liquid chromatography electrospray ionization time-of-flight mass spectrometry, flavan-3-ols by high-performance liquid chromatography with fluorescence detection, and the antioxidant activity by 2,2-diphenyl-1-picrylhydrazyl, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), and ferric reducing antioxidant power of the fruits of five species of genera Crataegus and Sorbus (Rosaceae). We found a total of 71 phenolic compounds from which 30 were identified in these berries for the first time. Crataegus monogyna and Crataegus laciniata revealed higher total phenolic and flavan-3-ol contents than the other species and the highest antioxidant activities. Therefore, the fruits evaluated have demonstrated to be important sources of bioactive compounds with huge potential for being used in nutraceutical or food scopes. Additional studies could be needed to evaluate the influence of the different production areas on the phenolic content. © 2023 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

Disposable biosensor based on nanodiamond particles, ionic liquid and poly-l-lysine for determination of phenolic compounds

This study describes the development of a highly sensitive amperometric biosensor for the analysis of phenolic compounds such as catechol. The biosensor architecture is based on the immobilization of tyrosinase (Tyr) on a screen-printed carbon electrode (SPE) modified with nanodiamond particles (ND), 1-butyl-3-methylimidazolium hexafluorophosphate (IL) and poly-l-lysine (PLL). Surface morphologies of the electrodes during the modification process were evaluated by scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDX). Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were used to investigate the electrochemical characteristics of the modified electrodes. Owing to the synergistic effect of the modification materials, the Tyr/PLL/ND−IL/SPE exhibited high sensitivity (328.2 μA mM−1) towards catechol with a wide linear range (5.0 × 10−8 – 1.2 × 10−5 M) and low detection limit (1.1 × 10−8 M). Furthermore, the method demonstrated good reproducibility and stability. The amperometric response of the biosensor towards other phenolic compounds such as bisphenol A, phenol, p-nitrophenol, m-cresol, p-cresol and o-cresol was also investigated. The analytical applicability of the biosensor was tested by the analysis of catechol in tap water. The results of the tap water analysis showed that the Tyr/PLL/ND−IL/SPE can be used as a practical and effective method for catechol determination.

Determination of phenolic compounds in water using a multivariate statistical analysis method combined with three-dimensional fluorescence spectroscopy.

Phenolic compounds are toxic chemical pollutants present in water. Three-dimensional fluorescence spectroscopy analysis is an effective and rapid method for real-time phenol monitoring in aquatic environments. However, similar chemical structures of phenols result in highly overlapping three-dimensional fluorescence spectra. Therefore, it is extremely difficult to analyze and quantify the concentration of components in a mixture system that includes two or more phenolic compounds. In this article, we study the mixed phenol system containing phenol, o-cresol, p-cresol, m-cresol, catechol, and resorcinol combined with excitation-emission matrix (EEM) fluorescence data. A multivariate statistical method called best linear unbiased prediction (BLUP) is proposed to analyze the spectra with the aim to achieve quantitative results and a trilinear decomposition algorithm called parallel factor analysis (PARAFAC) was used for comparison. Two experiments with different calibration samples were set to validate the effectiveness of BLUP through recovery, ARecovery (Average Recovery), AREP (Average Relative Error of Prediction), and RMSE (Root Mean Square Error). Overall, the average recovery of each component in experiment 1 and experiment 2 ranged from 95.91% to 111.62% and 82.91% to 129.02%, respectively. Based on the results of the experiments, the concentration of phenolic compounds in water can be quantitatively determined by combining three-dimensional fluorescence spectroscopy with the BLUP method.

Determination of phenolic compounds in Nasturtium Officinale by LC-MS / MS using different extraction methods and different solvents

This study aimed to determine the phenolic compound contents of Nasturtium officinale (N. officinale) extracts prepared with water, methanol, ethanol, and chloroform, as well as extracts prepared by evaporation and lyophilization, and parts of N. officinale by LC-MS/MS (Liquid Chromatography-Mass spectrometry/Mass spectrometry). The results obtained not only contribute to knowledge about the benefits of N. officinale but also show the effect of different solvents and different extraction methods on the determination of phenolic compounds. In addition to these results, the importance of the cold chain in the determination of some phenolic compounds has also been demonstrated. While it is observed that the number of phenolic components obtained from the extracts prepared with methanol and ethanol is high; It is observed that some phenolic compounds such as quinic acid, fumaric acid, aconitic acid, and p-coumaric acid dissolve very well in water and temperature is important.

Determination of Antioxidant Activity, Phenolic Compounds, and Toxicity of Methanolic and Ethanolic Extracts of Pink Pigmented Facultative Methylotrophs (PPFM) Bacteria Pigment

Pink-pigmented facultative methylotrophs bacteria are a plant’s surface inhabitant, especially at the leaf. They are known as Methylobacterium species. The antioxidant activity, phenolic compounds, and level of toxicity of this bacteria pigment have been studied. Recently, no previous research focused on the same bacterium found in Melicope lunu-ankenda (Gaertn.) T. G. Hartley, which is a component of the Malaysian ulam leaf. This study employed the 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric ion reducing antioxidant power (FRAP), and 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging assays, along with total phenolic content determination to assess the antioxidant activities of the methanolic and ethanolic pigment extract. Additionally, the consumption safety level of the pigment extract used brine shrimp lethality assay. From these findings, ethanolic pigment extract has a higher antioxidant capacity than methanolic extract. The DPPH half-maximal inhibitory concentration (IC50) value of methanolic pigment extract is higher than ethanolic extract (0.72 ± 0.04 mg/ml), but the IC50 value is vice versa for ABTS (4.59 ± 2.17 mg/ml). Furthermore, ethanolic extracts have a high FRAP assay value (1.09 ± 0.19 mg/mg of trolox equivalent at 0.78 mg/ml sample) and phenolic content (1.39 ± 0.07 mg/mg of gallic acid equivalent at 0.78 mg/ml sample) compared to methanolic pigment extracts. Fortunately, the methanolic and ethanolic pigment extract’s lethal concentration values (4.52 and 9.94 mg/ml) are considered safe for food application since their toxicity level is higher than 1 mg/ml.

Facile preparation of a Pt-ERGO composite modified screen-printed electrode for the sensitive determination of phenolic compounds

The mass production of screen-printed electrochemical devices with integrated electrodes has facilitated the widespread adoption of electroanalytical methods. The SPEs (screen-printed electrodes) overcome some obstacles associated with the use of conventional electrochemical cells, making them accessible to untrained operators. Despite their advantages, SPEs require activation/modification of the working electrode (WE) to enhance sensitivity. Nanomaterials, with metal nanoparticles (NPs) dispersed in polymers and/or carbon NPs has gaining popularity for this purpose. In this study, we describe a modification of carbon SPEs (SPCEs) using Pt NPs and reduced graphene oxide (ERGO). The Pt-ERGO@SPCE is prepared by galvanostatic reduction of drop-casted precursors directly onto the WE surface, eliminating complex synthetic steps and high temperatures. After optimizing Pt amount and reduction extent, the modified SPCEs were tested for detecting hydroquinone (HQ) and bisphenol A (BPA). DPV results show significantly increased sensitivity for the quantification of both compounds. The modified SPCEs demonstrates promising performance: precision (5 % HQ, 8 % BPA), detection limits (1.4 μM HQ, 4.6 μM BPA), sensitivity (1688 μA mM−1 HQ, 441 μA mM−1 BPA), and recoveries (98–113 % HQ, 98–104 % BPA). This simple electrode modification holds great potential, allowing the preparation of the sensor by personnel who may lack access to well-equipped laboratories, particularly in developing countries.

Determination of Phenolic Compounds in the Leaves Extract of Willow (Salix babylonica L.)

Determination of Phenolic Compounds in the Leaves Extract of Willow (Salix babylonica L.)

LC-MS/MS Analysis and Biological Activities of Methanol Extract from Sagina apetala Ard.

This is the first study on the investigation of phenolic compounds of methanol extract (ME) of Sagina apetala and examination of its cell-based antioxidant and antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa. The determination of phenolic compounds of ME was performed by LC-MS/MS and 25 main compounds were identified. For the cell-based antioxidant activity of ME, Vero cell line (Cercopithecus aethiops kidney epithelial, Monolayer) was used as the model cell line and ME was showed 61.22% cell viability. ME, also showed insignificant antibacterial activity against both gram-positive and gram-negative bacteria. In conclusion, this study in the species provides the basic data for future studies for the species.

Tef and fonio‐morphology, determination of amino acids, phenolic compounds and antioxidant capacity of two gluten‐free grains

SummaryGlobal demand for gluten‐free products is rising, however, currently most lack protein and are nutritionally insufficient. Tef and fonio, two nutritious gluten‐free cereals might provide a good alternative. To evaluate their composition, a detailed profiling of their free amino acid (AA) and phenolic composition was performed, using UPLC‐QqQ‐MS/MS, in addition to TPC and antioxidant activity. Grain morphology was also investigated. Our results reveal the presence of 18 AAs, including all essential, in both tef and fonio. l‐asparagine and l‐tryptophan were the most abundant, whereas cysteine and glutamine were absent. Levels of lysine were sufficient (4.39 and 6.72 mg g−1 DW for tef and fonio, respectively), but levels of methionine in fonio were low (0.45 mg g−1 DW). TPC was 0.87 and 0.38 mg GAE g−1 DW in tef and fonio, respectively. Eleven phenolic compounds were identified and quantified in both cereals, ranging from 2246 μg g−1 DW to trace levels, with higher levels observed in tef. ABTS Antioxidant activity was 0.25 and 0.15 mg TE g−1 DW for tef and fonio, respectively, typical for cereals and millets. Overall, our work implies that tef and fonio can make a healthful contribution to human nutrition, and specifically be incorporated into gluten‐free diet to increase protein and polyphenol consumption.

Magnetic nanoparticles and cationic surfactants for the extraction and determination of phenolic compounds in environmental and biological samples

Magnetic nanoparticles and cationic surfactants for the extraction and determination of phenolic compounds in environmental and biological samples

Determination of Parabens and Phenolic Compounds in Dairy Products through the Use of a Two-Step Continuous SPE System Including an Enhanced Matrix Removal Sorbent in Combination with UHPLC-MS/MS.

Dairy products can be contaminated by parabens and phenolic compounds from a vast variety of sources, such as packaging and manufacturing processes, or livestock through feed and environmental water. A two-step continuous solid-phase extraction (SPE) and purification methodology was developed here for the determination of both types of compounds. In the first step, a sample extract is passed in sequence through an EMR-lipid sorbent and an Oasis PRiME HBL sorbent to remove fat and preconcentrate the analytes for subsequent detection and quantification by UHPLC-MS/MS. This method enabled the determination of 28 parabens and phenolic contaminant with excellent recovery (91-105%) thanks to the SPE sorbent combination used. The proposed method was validated through the determination of the target compounds, and was found to provide low detection limits (1-20 ng/kg) with only slight matrix effects (0-10%). It was used to analyse 32 different samples of dairy products with different packaging materials. Bisphenol A and bisphenol Z were the two phenolic compounds quantified in the largest number of samples, at concentrations over the range of 24-580 ng/kg, which did not exceed the limit set by European regulations. On the other hand, ethylparaben was the paraben found at the highest levels (33-470 ng/kg).

Reversed phase‐high‐performance liquid chromatography simultaneous estimation of phenolic compounds in hydroalcoholic extract of Potentilla fulgens root

AbstractThe roots of Potentilla fulgengs have been utilized for a long time as an astringent and tonic for treating gum and tooth diseases, microbial contagions, inflammation, diabetes mellitus, and wounds and are rich in polyphenols and flavonoids. Authentication and constant excellence are the rudimentary limitations of herbal medicines and their complexity is a key challenge for quality control. High‐performance liquid chromatography has become an important technique for the quality control of herbal medicines because of its simplicity and uniformity. It can be applied for the identification, confirmation, and quality control of herbal medicines. So, an approach using reversed‐phase high‐performance liquid chromatography was developed and validated for the simultaneous determination of phenolic compounds in the hydroalcoholic extract (methanol:water::70:30% v/v) of Potentilla fulgens roots. The results revealed that ellagic acid was found in the highest amount (8.191 ± 0.463 mg/g) followed by rutin (5.429 ± 0.062 mg/g) and caffeic acid (3.032 ± 0.453 mg/g) among the studied phenolic compounds while quercetin was not detected. The validation parameters involved selectivity, linearity, range, limits of detection and quantitation, precision, and accuracy. All calibration curves were linear (R2 &gt; 0.99) within the range, and good precision (relative standard deviation &lt; 2.0%) and recovery (96.129%–117.110%) were found for all standards.