Determination Of Ethylenediaminetetraacetic Acid Research Articles

Determination of Ethylenediaminetetraacetic acid (EDTA) levels in surface waters by high performance liquid chromatography (HPLC)-Ultraviolet/Visible (UV/VIS) detector

Ethylenediaminetetraacetic acid (EDTA) is a chemical that is harmful to human health with its high solubility in water, which is used as a metal chelating agent in industries such as medicine, food, personal care product, agriculture. Thus, it is necessary to should be monitored in surface waters taken from dams supplying drinking and utility water. This work presents the applicability of the HPLC-UV/VIS system for the quantification of EDTA in surface waters based on the limit values of national and international legislation such as the Turkish Regulation on the Management of Surface Water Quality. The applicability of EDTA quantification in surface water was checked with validation study. The method validation consisted of selectivity, calibration curve linearity, limit of detection (LOD) and limit of quantification (LOQ), accuracy (recovery), and precision. In selectivity study, no peaks belonging to interfering compounds that would cause false-positive results were found in the chromatograms at the retention time of EDTA (5.773 min.). The linearity of EDTA was obtained ranging from 10 µg/L to 200 µg/L concentrations with the correlation coefficient of 0.9985 and the calibration curve equation of y=4659.4x-50223. The LOD and LOQ values of EDTA was 2.85 µg/L and 9.51 µg/L with the RSD of 5.36. In accuracy, the mean recovery of EDTA in surface water has been determined as 87.51 percent with an RSD of 6.11. The repeatability (RSD, %) varied from 5.44 % to 7.02 % with concentrations of 35.19 ± 1.91 μg/L and 17.11 ± 1.20 μg/L, whereas the reproducibility (RSD, %) was obtained at 3.45 % with the concentration of 34.13 ± 1.18 μg/L. In this study, the presence of EDTA was investigated in approximately 300 surface water samples and EDTA was found as positive in the concentration range of 11.17 µg/L to 52.14 µg/L in eleven real samples.

Determination of Ethylenediaminetetraacetic Acid from Swine Organs, Feces, Urine, and Plasma by Liquid Chromatography Tandem Mass Spectrometry

The study describes the development of a method for determination of ethylenediaminetetraacetic acid (EDTA) in various biological matrices after extraction as sample preparation using liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). The method is based on the complex formation of the excess of EDTA with FeIII. The excretion of EDTA in swine, treated with high doses of EDTA against dysentery, was monitored over a week by measuring its concentration in urine, plasma, and feces after stopping the administration of the medication. After slaughter, the EDTA concentration was measured also in the fatty tissue, kidney, liver, and muscle in order to determine the distribution of its residue between the tissues. The new method is fast, and the one-step sample preparation procedure may be regarded as derivatization. The method is applicable for low-concentration analysis with 3.0 ng mL−1 limit of detection and 10.0 ng mL−1 limit of quantification, while the relative standard deviation does not exceed 4.3 %.

Determination of ethylenediaminetetraacetic acid in foods by reversed-phase high-performance liquid chromatography

A convenient HPLC method for the quantitative determination of EDTA in foods was developed. EDTA in food samples was easily extracted with water by ultrasonication. After converting to Fe(III) complex in the presence of Fe(III) ions, EDTA was separated on a reversed-phase C30 column and detected with ultraviolet detection (260nm). Citrate and malate, which are present in many foods, also formed Fe(III) complexes but they did not interfere the chromatographic detection of EDTA. The method allowed determination of EDTA in foods at concentrations as low as 0.01mmol/kg. Good recoveries (95.2–101%) were obtained by the standard addition method on four samples with high repeatability (RSD, 0.8–3.4%). The method was successfully applied to the analysis of EDTA in carbonated drinks, jellies, canned beans, canned corn and food supplements.

Determination of ethylenediaminetetraacetic acid in nuclear waste by high-performance liquid chromatography coupled with electrospray mass spectrometry

EDTA is a chelating agent that has been used in decontamination processes. Its quantification is required for nuclear waste management because it affects the mobility of radionuclides and metals in environment and, thus, can harm the safety of the storage. Ion-pair chromatography coupled with electrospray mass spectrometry detection is a convenient method for quantitative analysis of EDTA but EDTA should be present as a single anionic chelate form. However, radioactive liquid wastes contain high concentrations of heavy metals and salts and consequently, EDTA is present as several chelates. Speciation studies were carried out to choose a metal cation to be added in excess to the solution to obtain a major chelate form. Fe is the predominant cation and Fe(III)–EDTA is thermodynamically favored but these speciation studies showed that ferric hydroxide precipitated above pH 2. Consequently, it was not possible to quantify EDTA as Fe(III)–EDTA complex. Therefore, Ni2+ was chosen but its use implied pretreatment with a base of the solution to eliminate Fe. Deuterated EDTA was used as tracer in order to validate the whole procedure, from the treatment with a base to the final analysis by HPLC–ESI-MS. This analytical method was successfully applied for EDTA quantification in two real effluents resulting from a nuclear liquid waste process. A recovery rate between 60 and 80% was obtained. The limit of detection of this method was determined at 34×10−9molL−1.

Determination of ethylenediaminetetraacetic acid in sea water by solid-phase extraction and high-performance liquid chromatography

The chelating agent EDTA is widely used, and as a result is showing up widely in the aquatic environment. Here we describe a preconcentration procedure for measuring EDTA concentration in sea water samples by HPLC. The procedure consists of forming an Fe(III) complex followed by solid-phase extraction using an activated carbon cartridge. After the preconcentration, EDTA was quantified by HPLC with ultraviolet detection (260 nm). The enrichment permitted the determination of EDTA at concentrations as low as 1 nM. Good recoveries were obtained for both brackish and full-strength sea water with high repeatability (RSD < 6%). The method was applied to sea water samples taken from near the mouth of the Oyabe River in Japan.

Determination of ethylenediaminetetraacetic acid at very low concentrations by high-performance liquid chromatography coupled with electrospray mass spectrometry

Ethylenediaminetetraacetic acid (EDTA) is a metal complexing agent which is commonly used for removing metallic surface contaminations. But its presence in aqueous industrial effluents or in wastes, by example those which are generated from nuclear power facilities, is forbidden or strictly regulated by the legislation. The implementation of high-performance liquid chromatography coupled with mass spectrometry (MS) via an electrospray interface has exhibited a powerful capacity in the measurement of this complexing agent, in the form of its iron complex. The complex is eluted through a reversed stationary phase under slightly acidic conditions and the detection is performed by mass spectrometry in the single ion monitoring mode. This technique has allowed us to reach a detection limit of about 1 μg/l in EDTA for only 20 μl injected without any previous preconcentration, and to solve some analytical interferences observed in the case of industrial effluents with other analytical techniques such as ion chromatography.

Determination of ethylenediamine tetraacetic acid in injection forms by ion-pair chromatography

A high performance liquid chromatographic method was developed for the determination of ethylenediamine tetraacetic acid (EDTA) in injection forms. The method consists of direct extraction of the samples with ethyl acetate; the organic layers were evaporated to dryness and further diluted to a 0.025% (w/v) copper nitrate in order to achive the formation of the EDTA–copper solution complex. The chromatographic separation was performed on a C8 Hypersil column. The mobile phase consisted of a mixture of acetonitrile–0.015 M tetrabutylammonium hydroxide (10:90, v/v), (pH* 7.0) pumped at a flow rate of 1.5 ml min −1. The UV detector was operated at 300 nm. Correlation coefficients of the calibration graphs were better than 0.9995, relative standard deviation was less than 2.5%. Detection limit of EDTA was found to be 1.97 μg ml −1.

Laser induced resonance energy transfer — a novel approach towards achieving high sensitivity in capillary electrophoresis Part B: Applications for environmental testing

Capillary electrophoresis with laser induced resonance energy transfer detection is used for direct determination of ethylenediamine tetraacetic acid (EDTA), 1,2-diaminopropane-N,N,N′,N′-tetraacetic acid (DPTA) and (±)- trans-1,2-diaminocyclohexane-N,N,N′,N′-tetraacetic acid (DCTA) in aqueous samples. The method involves CZE separation of salicylate-Tb-EDTA, salicylate-Tb-DPTA and salicylate-Tb-DCTA ternary complexes at alkaline pH. The highly efficient transfer of energy from salicylate to terbium in these complexes facilitated a sensitive detection. Detection limits in the 1·10 −7 M range are easily achieved. A 325 nm HeCd laser is used for excitation and terbium luminescence is monitored at 547 nm. The potential application of this method in analyzing these acids in a complex radioactive waste is discussed.

Determination of chelating agents in Hanford tank waste simulant by capillary zone electrophoresis

AbstractA capillary electrophoretic method has been developed which achieves rapid quantitative separation and determination of ethylenediaminetetraacetic acid, N‐(2‐hydroxyethyl)ethylenediaminetriacetic acid, diethylenetriaminepentaacetic acid, ethylenediaminediacetic acid, and nitrilotriacetic acid in Hanford tank waste simulant (TWS). Direct UV detection of Cu2+/chelator complexes is used to achieve enhanced sensitivity. The qualitative and quantitative reliability of the method and the quality of separations, as given by efficiency and resolution, is presented. In addition, the limits of detection and linearity of detector response with concentration are given for each chelator. The selection of Cu as the UV‐absorbing species over other transition metals present in the waste is discussed.

Effect of surface tension of the matrix on fast-atom bombardment mass spectral determination of ethylenediaminetetraacetic acid

The importance of the influence of surface activity on the mass spectra obtained from particle bombardment of liquid surfaces has long been known. In this work the fast-atom bombardment (FAB) mass spectral determination of ethylenediaminetetraacetic acid (EDTA) in several liquid matrices is investigated. Differences in the relative molar response observed for glycerol, thioglycerol and triethanolamine are discussed in terms of the surface tension of these liquids. The surface tensions of the pure liquid matrices and the EDTA solutions have been determined at 25 and 70 °C using the axisymmetric drop-shape analysis profile (ADSAP) technique. EDTA solutions have a higher surface tension than the corresponding pure liquid matrices, indicating that EDTA molecules are concentrated mainly in the bulk. Furthermore, the order observed for the intensity of the ion signal obtained with the three matrices, glycerol > thioglycerol > triethanolamine, follows the order of the surface tension which is maximum for glycerol and minimum for triethanolamine. We conclude that the surface concentration of EDTA is at a maximum in the liquid matrix with the highest surface tension, yielding an optimized mass spectrum.

Determination of ethylenediaminetetraacetic acid by flame atomic absorption spectrometry with a chelating ion-exchange flow injection conversion system

A flow injection method (FI) has been developed for the determination of ethylenediaminetetraacetic acid (EDTA) by flame atomic absorption spectrometry (FAAS). In the first step a copper(II) regenerant solution was injected on to an on-line column packed with Chelex-100 resin. The excess of copper ion, not retained on the column, was washed by an ammonia solution carrier. In the second step an EDTA sample was injected. The analyte displaced an equivalent amount of copper(II) from the chelating column. Eluted copper was determined by an FAA spectrometric detector positioned downstream. The signal obtained was proportional to the concentration of EDTA present in the injected sample. Using this FI-FAAS conversion method the detection limit was 0.1 µg ml–1. The precision of the technique was better than a relative standard deviation of 1.5% at 0.50 µg ml–1 levels, with a throughput of 45 samples h–1. The effect of sample volume and of ionic and organic ligand interferents on the FI-FAAS signals was studied. Only glycylglycine was found to interfere significantly.

Indirect Trace Determination of Ethylenediminetetraacetic Acid (Edta) in Water by Potentiometric Stripping Analysis

Abstract An analytical method for indirect trace determination of ethylenediaminetetraacetic acid (EDTA) in water, by potentiometric stripping analysis is described. Excess Bi(III) was added to form a 1:1 complex with EDTA at pH 2.3. The uncomplexed Bi(III) was then deposited on a glassy carbon electrode at a potential of −0.40 V vs. SCE and subsequently stripped potentiometrically using potassium dichromate as oxidant. The stripping time of uncomplexed Bi(III) was recorded. The Concentration of EDTA in the sample was determined from the concentration of added Bi(III) and the potentiometrically stripped Bi(III) at −0.4 V by the standard addition method. The relative standard deviation for EDTA concentration of 95 ppb and 4.5 ppb was 1.9% and 2.6%, respectively. The detection limit was about 1 ppb EDTA for a deposition time of 3 minutes.

Determination of ethylenediaminetetra-acetic acid in aqueous rinses of detergent-washed rubber stoppers of pharmaceutical vials using solid-phase extraction and capillary gas chromatography

A fused silica capillary gas chromatographic method is presented for the determination of traces of ethylenediaminetetra-acetic acid (EDTA) in aqueous rinses of rubber stoppers of pharmaceutical vials after treatment with detergents containing EDTA. Isolation and enrichment of EDTA from the aqueous medium is achieved using a commercially available strong anion-exchange solid-phase extraction cartridge, transformed to the formate form. A 2.0-ml volume of methanolic HCl is used for both elution of EDTA from the extraction column and formation of the tetramethyl ester derivative. With the incorporation of a methanolic wash to eliminate interfering components prior to elution with methanolic HCl, a limit of detection of 25 ng EDTA per ml water with a non-selective flame ionization detector is possible.

Determination of ethylenediaminetetra-acetic acid in foods by colorimetry.

Determination of ethylenediaminetetra-acetic acid in foods by colorimetry.

Colorimetric determination of ethylenediaminetetra-acetic acid in foods.

A simple colorimetric method is described for the determination of ethylenediaminetetra-acetic acid (EDTA) in three kinds of foods. The samples were homogenized with 0.1 N-NaOH, then subjected to equilibrium dialysis against 0.02 N-NaOH. EDTA in the dialyzate was converted into a Cu-EDTA complex by the addition of CuSO4. Afterwards, this solution was subjected to the determination of both free and total (free plus complexed) copper ion. EDTA was calculated from the difference between the absorbances (477 nm) obtained with free and total copper ions. Analyses of mayonnaise, dressings and canned mushroom samples with EDTA added at the 1-mM level showed a recovery of greater than 98%.

Determination of ethylenediaminetetraacetic acid in boiler water by liquid chromatography

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTDetermination of ethylenediaminetetraacetic acid in boiler water by liquid chromatographyDavid L. Venezky and Walter E. RudzinskiCite this: Anal. Chem. 1984, 56, 2, 315–317Publication Date (Print):February 1, 1984Publication History Published online1 May 2002Published inissue 1 February 1984https://doi.org/10.1021/ac00266a050RIGHTS & PERMISSIONSArticle Views179Altmetric-Citations42LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (384 KB) Get e-Alerts Get e-Alerts

Determination of Ethylenediaminetetraacetic Acid and Nitrilotriacetic Acid as Their Iron(III)-Complexes by Reversed Phase High Performance Liquid Chromatography

Abstract The iron(III) complexes with ethylenediaminetetraacetic acid (EDTA, H4edta) and nitrilotriacetic acid (NTA, H3nta) were separated by the ion-association reversed phase high performance liquid chromatography. The retention behavior of iron(III)–nta complex on the column (4.6 mm i.d., 25 cm long) packed with ZORBAX-ODS was seriously affected by the pH of eluent composed of phosphate buffer solution containing tetrabutylammonium bromide and acetonitrile. A mixture of edta and nta in a synthetic sample solution containing the commonly present metal ions was converted to the corresponding iron(III) complexes and determined successfully by the present method.

Determination of ethylenediaminetetraacetic acid and related amino‐carboxylic acids by gas chromatography

Determination of ethylenediaminetetraacetic acid and related amino‐carboxylic acids by gas chromatography

Reverse Phase Ion Pair High Pressure Liquid Chromatographic Determination of Ethylenediaminetetraacetic Acid in Crabmeat and Mayonnaise

A method is described for the determination of ethylenediaminetetraacetic acid (EDTA) in crabmeat and mayonnaise. EDTA is extracted from the food sample with water and converted to its copper chelate, which is then quantitated by reverse phase ion pair high pressure liquid chromatography with ultraviolet detection. Maximum sensitivity is obtained with detection at about 254 nm; higher wavelengths may be used for enhanced specificity. Cleanup procedures for crabmeat and mayonnaise were improved by using a radiotracer method. Analyses of crabmeat and mayonnaise samples spiked at 3 different levels showed greater than 90% recovery of EDTA.

Gas-Liquid Chromatographic Determination of Ethylenediaminetetraacetic Acid in Pickled Vegetables

Abstract A method has been developed for the determination of ethylenediaminetetraacetic acid in pickled vegetables after conversion to its tetramethyl ester. The ester is identified by gas-liquid chromatography and confirmed by gas-liquid chromatography-mass spectrometry. Recoveries from pickled beet samples spiked at 20-250 ppm were 87-74%.