Determination Of Emodin Research Articles

Research on the determination of emodin in Xiaoyan granulas

Objective To establish a method for the determination of emodin in Xiaoyan granulas by high performance liquid chromatography (HPLC). Methods Took inertsil ODS-3 (250 mm × 4.6 mm, 5 μm) as the chromatographic separation the mobile phase was acetonitrile-0.1% phosphoric acid (80:20), the detection wavelength was 254 nm, the flow rate was 1.0 ml/min, and the column temperature was at 40 ℃. Results The linear range of emodin was 0.034 6-0.432 0 μg (r=0.999 9). The average recovery was 94.02% (RSD=1.29%, n=6). Conclusions This method is simple, accurate, which could be used repeatedly and can be used as the emodin determination method for Xiaoyan granulas. Key words: Emodin; Xiaoyan granulas; High performance liquid chromatography; Assay (TCD); Quality control

Simultaneous determination of emodin, chrysophanol, parietic acid, and magnolol in Fructus Cannabis Bolus by high performance liquid chromatography

Objective To simultaneously determinate 4 effective components in Fructus Cannabis Bolus by high performance liquid chromatography (HPLC). Methods The column was ALLTIMA-C18 column (250 mm × 4.6 mm, 5 μm). The mobile phase was methanol (A)-0.1% phosphoric acid aqueous solution (B) in gradient elution program. The flow rate was 1.0 ml/min, the column temperature was 30℃, and the injection volume was 10 μl. The detection wavelength was set at 254 nm. Results The linear ranges of 4 effective components of emodin, chrysophanol, parietic acid, and magnolol were 9.05-81.26 μg (r=0.999 8), 13.28-119.3 μg (r=0.997 6), 8.30-75.40 μg (r=0.998 7), 6.30-61.79 μg (r=0.996 5), respectively; the average recoveries and RSDs of emodin, chrysophanol, parietic acid, and magnolol were 99.25%, 99.46%, 99.51%, 99.86%, respectively. The RSDs of emodin, chrysophanol, parietic acid, and magnolol were 2.04%, 3.23%, 1.84%, and 2.42%, respectively. Conclusions The HPLC can be used as an effective and feasible method for the determination of emodin, chrysophanol, parietic acid, and magnolol in Fructus Cannabis Bolus. The method is simple, quick, and reproducible, and can be used for the quality control of Fructus Cannabis Bolus. Key words: Assay (TCD); Fructus Cannabis Bolus; Emodin; Chrysophanol; Rhein; Magnolol; High performance liquid chromatography

Study on quality standard of Tongfu navel plaster

Objective To establish the quality standard of Tongfu navel plaster. Methods Rhubarb, Magnolia and Citrus aurantium were identified by thin-layer chromatography（TLC）. HPLC method was established for the determination of emodin and rhein. The chromatographic column was SunFire C18 column （4.6 mm×250 mm, 5 μm） with methanol-0.1%H3PO4（85∶15） as the mobile phase. The flow rate was 1.0 ml/min, column temperature was at 30 ℃, and the detection wavelength was 254 nm. Results Within the range of 0.144-1.728μg, emodin presented a fine linear relationship, r=0.999 6. Within the range of 0.016-0.208μg, rhein presented a fine linear relationship, r=0.999 6. The recovery of rhein and emodin was 98.18%and 98.75% with RSD 0.98% and 0.91% respectively. Conclusion The methods are reliable, accurate, reproducible, and can be used for the quality control of Tongfu navel plaste. Key words: Tongfu navel plaster; Quality standard; TLC; HPLC; Rhein; Emodin

Determination of emodin in L-02 cells and cell culture media with liquid chromatography–mass spectrometry: Application to a cellular toxicokinetic study

The emodin-involved hepatotoxicity has been gaining increasing attention. The purpose of the present study was to evaluate the cytotoxicity of emodin on cultured human liver cells (L-02) and predict the possible relation between its cytotoxicity and cellular toxicokinetics. Cell viability and cell damage were assessed by Cell Counting Kit-8 (CCK-8) assay and phase-contrast microscopy, respectively. Cytotoxicity tests demonstrated a concentration- and time-dependent toxic effect of emodin on L-02 cells. Furthermore, emodin at concentration of 30μM led to a significant apoptosis in a time-dependent manner supported by the morphological changes of drug-treated cells. In addition, to elucidate the toxicokinetic characteristics of emodin, a highly sensitive and selective liquid chromatography-mass spectrometry (LC-MS) method was employed and validated for detecting the dynamic alteration of emodin in cells and cell culture media. The proposed method appeared to be suitable for the analysis of emodin with desirable linearity (r(2)>0.99), and satisfying precision being less than 8.7%. The range of recoveries of this method was 90.2-101.9%. The preliminary cellular toxicokinetic study revealed a time-dependent intracellular accumulation of emodin, which was consistent with its in vitro toxic effects. These findings confirmed the cytotoxicity of emodin against L-02 cells and displayed the cytotoxic manner of emodin in terms of its cellular uptake and accumulation in L-02 cells.

Determination of emodin by hexadecyl trimethyl ammonium bromide sensitized fluorescence quenching method of the derivatives of calix[4]arene

The fluorescence quenching effect of emodin (EMO) on the derivatives of p-tert-butyl-calix[4]arene with o-phenanthroline (TBCP) in 1.0% hexadecyl trimethyl ammonium bromide (CTAB) medium was investigated. The fluorescence of TBCP was quenched by EMO due to the formation of the weak fluorescent inclusion complex (EOM-TBCP), and the fluorescence quenching (ΔF=FTBCP–FEMO–TBCP) was sensitized in CTAB. Under the optimal conditions, the linear range of calibration curve for the determination of EMO was 1.17–23.40μg/mL. The detection limit estimated and RSD was 0.34μg/mL, 3.63% (n=3, c=4.74μg/mL). The quantum yield Yu of TBCP was approximately 2.0 times higher in the presence of CTAB than that in the absence of CTAB. The method has been applied for the determination of EMO in samples with satisfactory results.

Determination of Nanogram Quantities of Emodin in Pharmaceutical Preparations and Biofluids by Luminol-Myoglobin Chemiluminescence System

Based on the quenching effect of emodin on the luminol-myoglobin (Mb) reaction, a sensitive method for the determination of nanogram level emodin by flow injection chemiluminescence (FI-CL) is presented for the first time. It was found that the CL intensity from luminol-Mb system could be inhibited in the presence of emodin, and the decrement of CL intensity was linearly proportional to the logarithm of emodin concentration in the range of 0.5– (R= 0.9956) with the detection limit of (3σ). At a flow rate of , a complete determination of emodin, including sampling and washing, could be accomplished in 0.5 min with the relative standard deviations (RSDs) of less than 3.5% (n= 5). The proposed method was successfully applied to the determination of emodin in pharmaceutical preparations and human serum samples. The possible CL mechanism of luminol-Mb-emodin reaction was explained.

Determination of emodin in Dabaidu capsule by HPLC

Objective To establish a HPLC method for the determination of emodin in Dabaidu capsule. Methods The chromatographic system was adopted C18 column, using methanol -0.1% phosphoric acid (70∶30) as mobile phase. The content was detected at a waveleng of 254 nm and the flow rate was 1.0 ml/min.Results The emodin have a good tincar rolatim in the range of 6.181 ～ 18.54 μg/ml (r=0.9999), the average recovery rate was 98.93% and RSD = 0.65% (n = 6) . Conclusion The established method is accuracy,sensitive and simple. Key words: Emodin; HPLC; Determination

Determination of emodin in shenqu weitong capsule by rp-hplc an injection volume of 10

目的 采用反相高效液相色谱法测定神曲胃痛胶囊中大黄素的含量.方法 采用Hypcrsil BDSC18柱(200mm×4.6mm,5μm),流动相:甲醇-0.1%磷酸溶液(85:15),流速:1.0ml·min-1,检测波长:254nm,柱温:30℃,进样量:20μ1.结果 神曲胃痛胶囊的线性范围为0.0 32～0.32 μg(r＝0.9995),平均回收率为96.78%,RSD＝1.71%.结论 反相高效液相色谱法测定神曲胃痛胶囊中大黄素含量,方法准确、操作简便、结果可靠,可用于神曲胃痛胶囊的质量控制。

Optimization of extraction and determination of emodin from Polygonum cuspidatum Sieb. et Zucc. products by HPLC-DAD

A uniform experimental design procedure was used to investigate the effects of some operating parameters on the extraction of emodin from Polygonum cuspidatum Sieb. et Zucc. products. Variables tested were volume ratio of material to solvent, size of material, extraction time and temperature and composition of extraction solvent (mixtures of acetone-water). Each variable was tested at seven levels; 7 experiments were performed in random order. Analyses of the extracts were performed by high-performance liquid chromatography with diode array detection(HPLC-DAD). Analytical responses were processed by using a forward regression analysis, in order to find polynomial function describing the relationship between variables and responses. For all the analytes the experimental conditions for providing the highest extraction yield inside the experimental domain considered were found. Finally, a simple, rapid and accurate analytical method was developed for the determination of emodin by high performance liquid chromatography. The separation is achieved within 25 min on an ODS column using methanol and water as gradient mobiles. Emodin can be quantified by using external standard method detecting at 436 nm. Good linearity is obtained with correlation coefficient exceeding 0.9986 and the detection limit and the quantification limit are 1.53 and 3.23 mg/L respectively. This method shows good reproducibility for the quantification of the emodin with intra-day and inter-day relative standard deviation less than 2.3% and 5.6% respectively. Under optimized extraction conditions, the recovery of the standard is 96.5%. The validated method is successfully applied to quantify the emodin in seven Polygonum cuspidatum sieb. Et zucc. products, which provided an idea for the pre-treatment of determination of active compounds in traditional Chinese medicines.

Emodin, A Naturally Occuring Anthraquinone: Its Isolation and Spectrophotometric Determination in Rumex Cyprius Plant

Abstract A new method for isolation and specrophotometric determination of emodin is presented. Emodin was isolated by thin layer chromatography (tlc) and column chromatography (cc) techniques, as an orange long crystalline substance. Emodin exhibits two absorption maxima, at 420 and 520 nm. Stability of the color and the effect of pH were studied. Beer's law is obeyed in the range 2–30 ppm. The method is applied to the determination of emodin in roots, stems, and leaves of Rumex cyprius plant.

Determination of emodin in feeds.

Determination of emodin in feeds.