Abstract

Objective To establish a method for the determination of emodin in Xiaoyan granulas by high performance liquid chromatography (HPLC). Methods Took inertsil ODS-3 (250 mm × 4.6 mm, 5 μm) as the chromatographic separation the mobile phase was acetonitrile-0.1% phosphoric acid (80:20), the detection wavelength was 254 nm, the flow rate was 1.0 ml/min, and the column temperature was at 40 ℃. Results The linear range of emodin was 0.034 6-0.432 0 μg (r=0.999 9). The average recovery was 94.02% (RSD=1.29%, n=6). Conclusions This method is simple, accurate, which could be used repeatedly and can be used as the emodin determination method for Xiaoyan granulas. Key words: Emodin; Xiaoyan granulas; High performance liquid chromatography; Assay (TCD); Quality control

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call

Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.