Determination Of Acid Phosphatase Activity Research Articles

Spectrofluorimetric determination of acid phosphatase activity

A spectrofluorimetric method for determination of acid phosphatase activity in serum using α-naphthyl phosphate as substrate is described. The inhibition of prostatic a-naphthyl phosphatase by l-tartrate has been shown to be competitive, and values for inhibitor and substrate constants are reported. In serum from a case of cancer of the female breast a considerable acid phosphatase activity towards α-naphthyl phosphate has been found, and this activity diners from that of the prostatic type enzyme in resisting inhibition by tartrate.

Determination of acid phosphatase activity in cells of prostatic tumours.

A SIMPLE technique by which cell material can be easily secured from prostatic tumours with the aid of transrectal aspiration biopsy has recently been described1. The material thus obtained was shown to consist largely of plugs of epithelial cells with occasional admixture of blood. In the present work these tissue fragments were isolated for determination of their acid phosphatase activity.

Microdetermination of acid phosphatase a study of the reaction of King and Armstrong

A micromethod is described for the determination of acid phosphatase activity with a phenylphosphate substrate on 10 μl of serum or on isolated microtome sections. There is a linear relationship between the concentration of enzyme and the amount of phenol liberated, even for high values of the latter. The relation with the time is not linear. When the reaction of King and Armstrong is used for sera, part of the reagent is fixed by the proteins, and this proportion varies in the blanks and in the incubated specimens. The corresponding variations of color formation are an important cause of error in the clinical determinations on sera, but this error is negligible for tissue studies. The rate of color development increases with the pH and the temperature. These factors are discussed fro proper application of the micromethod to enzyme detreminations in tissue sections.