Abstract
A micromethod is described for the determination of acid phosphatase activity with a phenylphosphate substrate on 10 μl of serum or on isolated microtome sections. There is a linear relationship between the concentration of enzyme and the amount of phenol liberated, even for high values of the latter. The relation with the time is not linear. When the reaction of King and Armstrong is used for sera, part of the reagent is fixed by the proteins, and this proportion varies in the blanks and in the incubated specimens. The corresponding variations of color formation are an important cause of error in the clinical determinations on sera, but this error is negligible for tissue studies. The rate of color development increases with the pH and the temperature. These factors are discussed fro proper application of the micromethod to enzyme detreminations in tissue sections.
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