The study was conducted to evaluate the epidemiological of toxoplasma gondii in local cows in Salah Adeen, and its effect to renal and liver function. One hundred twenty six sample of serum were collected from cows n different cows farm in Salah Adeen, include Samarra (60 sample), Al-Mutasim (28 sample), Baled (23 sample) and Aldejil (15 sample), from the period between July 2019 until February 2020. The study include detection of serum anti T. gondii IgG antibody by using ELISA Toxoplasma IgG kit, and also determination of serum liver enzymes activity which include (Transaminases enzymes: aspartate transaminase-AST and alanine transaminase-ALT) and also the concentration of serum total protein-TP, albumin, globulin, urea and creatinine. The results indicate that the total percentage for the prevalence of T.gondii between cows in Saleh Eldean was 51.58%, distributed according to the region (68.33% in Samarra, 50% in Al-Mutasim, 26.08% in Baled and 26.66 in Aldejil). While the total percentage for infection with the parasite according to the gender of animals were 58.33% (28 from 48) in male animal and 47.43% (37from 78) in female animal. The results also showed that the level of TP, Albumin, globulin were significantly higher P≤0.05 in sera of infected animals, and also the activity of liver enzymes (AST and ALT) were significantly higher P≤0.05 in sera of infected animals with T.gondii with no significant difference in the level of urea and creatinine in sera of infected group as compared with non-infected group as control group. From all the above results we can conclude that the prevalence of T. gondii was high among animal under investigation, and this infection may be effect to the physiological function especially liver function.