Detection Of Zearalenone Research Articles

A ratiometric fluorescence aptasensor based on photoinduced electron transfer from CdTe QDs to WS2 NTs for the sensitive detection of zearalenone in cereal crops

Semiconductor quantum dots (QDs) and tungsten disulfide nanosheets (WS2 NTs) have been widely studied in photocatalysis and photoelectrochemistry as representative electron donor–acceptor pairs but rarely in fluorescence sensing. In this study, we investigated the effect of WS2 NTs on the fluorescence signal of cadmium telluride (CdTe) QDs by grafting an aptamer as a bridge between them. The corresponding quenching mechanism was systematically explored, and results described the photoinduced electron transfer (PET) from excited CdTe QDs to WS2 NTs. Based on these results, a novel ratiometric fluorescence aptasensor was developed for zearalenone (ZEN) determination by innovatively introducing exonuclease I to digest the aptamer of ZEN and control the PET between CdTe QDs and WS2 NTs. The designed aptasensor exhibited an acceptable linear range and detection limit (0.1 pg mL−1), and superior accuracy and selectivity. This ratiometric fluorescence aptasensor was also used to monitor ZEN in rice and corn flour.

Recent advances in the highly sensitive determination of zearalenone residues in water and environmental resources with electrochemical biosensors.

Zearalenone (ZEN), a significant class of mycotoxin which is considered as a xenoestrogen, permits, similar to natural estrogens, it's binding to the receptors of estrogen resulting in various reproductive diseases especially, hormonal misbalance. ZEN has toxic effects on human and animal health as a result of its teratogenicity, carcinogenicity, mutagenicity, nephrotoxicity, genotoxicity, and immunotoxicity. To ensure water and environmental resources safety, precise, rapid, sensitive, and reliable analytical and conventional methods can be progressed for the determination of toxins such as ZEN. Different selective nanomaterial-based compounds are used in conjunction with different analytical detection approaches to achieve this goal. The current review demonstrates the state-of-the-art advances of nanomaterial-based electrochemical sensing assays including various sensing, apta-sensing and, immunosensing studies to the highly sensitive determination of various ZEN families. At first, a concise study of the occurrence, structure, toxicity, legislations, and distribution of ZEN in monitoring has been performed. Then, different conventional and clinical techniques and procedures to sensitive and selective sensing techniques have been reviewed and the efficient comparison of them has been thoroughly discussed. This study has also summarized the salient features and the requirements for applying various sensing and biosensing platforms and diverse immobilization techniques in ZEN detection. Finally, we have defined the performance of several electrochemical sensors applying diverse recognition elements couples with nanomaterials fabricated using various recognition elements coupled with nanomaterials (metal NPs, metal oxide nanoparticles (NPs), graphene, and CNT) the issues limiting development, and the forthcoming tasks in successful construction with the applied nanomaterials.

Dual-Enzyme-Based Signal-Amplified Aptasensor for Zearalenone Detection by Using CRISPR-Cas12a and Nt.AlwI.

Zearalenone (ZEN) is harmful to animals and human beings, so it is very important to develop a rapid and sensitive method for the detection of ZEN. In this paper, we proposed a novel ZEN-monitoring method using two aptamers as recognition elements and EnGen LbaCas12a and Nt.AlwI nicking endonuclease as signal amplifiers. When ZEN was present, it bound to the aptamer Z0 and, Z1 was released into solution. The solution was then separated and the Nt.AlwI enzyme was added in order to form a nicking-enzyme cycle, thereby producing large amounts of the ssDNA Z3 for 30 min. The Z3 formed a CRISPR-Cas12a-Z3 complex with CRISPR-Cas12a, activated the trans-cleavage ability of Cas12a, cleaved the Quenched Reporter for 20 min, and underwent fluorescence recovery. The aptasensor was able to sensitively detect ZEN in the linear range of 1–1000 pg/mL, with a detection limit as low as 0.213 pg/mL. The detection time lasted for 2 h. Additionally, this detection technology can also be used to monitor other hazards.

Alkaline phosphatase-triggered dual-signal immunoassay for colorimetric and electrochemical detection of zearalenone in cornmeal

Zearalenone (ZEN), as one of the most important foods and feeds pollutant mycotoxins, is threatening human and animal health. Here, we have developed a dual-signal immunoassay triggered by alkaline phosphatase (ALP) for detecting ZEN in cornmeal. The immobilized ZEN-bovine serum albumin and free ZEN in the sample were competitively bound to an anti-ZEN monoclonal antibody (McAb). Then, the ALP labeled goat anti-mouse IgG (ALP-IgG) combined with the McAb. ALP catalyzed ascorbic acid 2-phosphate to produce L-ascorbic acid (AA). AA converted potassium ferricyanide (K3[Fe(CN)6]) to potassium ferrocyanide (K4[Fe(CN)6]), which reacted with ferric ion (III) to promote the formation of Prussian blue nanoparticles (PB NPs). Consequently, the solution appeared multicolor changes. Meanwhile, PB NPs had a maximum absorption peak at 700 nm. It could be monitored by an UV–vis spectrometer. As an electron transfer medium, K3[Fe(CN)6] was consumed gradually along with PB NPs formation. Therefore, electrochemical detection was also used for detecting ZEN. Under the optimum conditions, the logarithm of ZEN concentration showed a good linear relationship with the absorbance from 0.2 to 0.8 ng/mL (R2 = 0.987) and the differential pulse voltammetry peak current from 0.125 to 0.5 ng/mL (R2 = 0.993). The limits of detection of colorimetric and electrochemical methods were 0.04 and 0.08 ng/mL, respectively. The recovery rates of ZEN from cornmeal samples were in the range of 80–120%, and relative standard deviations were lower than 10%. The results demonstrated that the dual-signal immunoassay has a high sensitivity for ZEN detection in corn samples.

Organic Cation Receptor for Colorimetric Lateral Flow Device: Detection of Zearalenone in Food Samples.

As per the WHO reports, it has been estimated that almost 25% of food crops contain mycotoxins as the major contaminant. In this work, we developed a paper-based colorimetric lateral flow device (CLFD) impregnated with an organic cation receptor (OCR) for sensitive and selective detection of zearalenone (ZEN). Various techniques such as ultraviolet (UV)-visible absorption, cyclic voltammetry, and fluorescence spectroscopy were used for the detection of mycotoxins, and it was observed that OCR shows sensitivity and selectivity toward zearalenone (ZEN) only, irrespective of any other analytes. Furthermore, the colorimetric test revealed that the developed OCR shows a change in color with the addition of ZEN from greenish-gray to blue that is visible to the naked eye. The quantification of ZEN was also achieved using RGB analysis and compared with UV-visible spectroscopy data. Further, for the on-site detection of ZEN, a paper-based CLFD was also developed and used to evaluate the spiked corn sample containing ZEN, and it provided significant results with a limit of detection (LOD) of 0.31 nM (3σ method), good linearity (R2 = 0.9702), good reproducibility (SD = ±6%, triplicate), and good recovery of ZEN of 95-102% with a variation coefficient (VC) varying from 1.56 to 4.62%. Therefore, the device has the potential to check the mycotoxin toxicity in food products and is helpful in remote and developing areas.

Visual detection of aflatoxin B1 and zearalenone via activating a new catalytic reaction of "naked" DNAzyme.

Here, we have found for the first time that the catalytic activity of "naked" DNAzyme, a single-stranded G-quadruplex DNAzyme (S.DNAzyme), can be modulated by aflatoxin B1 (AFB1) and zearalenone (ZEN). In fact, S.DNAzyme can mimic the activity of horseradish peroxidase to perform oxidation of 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) to colored ABTS˙+ (dark green). This catalytic activity can be inhibited upon addition of AFB1, leading to color fade of the solution. But with ZEN, the solution color will change to a yellowish-brown or yellow color. Thus, we have exploited this finding to achieve label-free, naked-eye detection of AFB1 or ZEN, and have explored the possible detection mechanisms. This approach is able to detect AFB1 and ZEN concentrations as low as 0.18 μM and 0.29 μM, respectively. Even in real samples, the limit of detection values of these two analytes are lower than 3 μM. Notably, S.DNAzyme cross-reacted with three other tested aflatoxins, including aflatoxin B2, aflatoxin G1 and aflatoxin M1, revealing the potential for a broadly applicable method to identify the family of aflatoxins. The AFB1- or ZEN-triggered new catalytic reaction between "naked" DNAzyme and ABTS may offer new opportunities for on-site visual detection of mycotoxins.

Application of Multispectral Imaging Combined with Machine Learning for Rapid and Non-Destructive Detection of Zearalenone (Zen) in Maize

Application of Multispectral Imaging Combined with Machine Learning for Rapid and Non-Destructive Detection of Zearalenone (Zen) in Maize

A sensitive MnO2 nanosheet sensing platform based on a fluorescence aptamer sensor for the detection of zearalenone.

An aptamer sensor based on manganese dioxide (MnO2) nanosheets was developed for the detection of zearalenone (ZEN). The ZEN aptamer was modified at the 5'-end by a 6-carboxyfluorescein (6-FAM) fluorophore with self-assembly on MnO2 nanosheets. Interaction of the 6-FAM fluorophore at the 5'-end of the ZEN aptamer with the MnO2 nanosheet lowered fluorescence (FL) intensity due to fluorescence resonance energy transfer (FRET). The introduction of ZEN into the sensing system resulted in hybridization with the ZEN aptamer, forming a stable G-quadruplex/ZEN, which exhibited a low affinity for the MnO2 nanosheet surface. The distance between the 6-FAM fluorophore and MnO2 nanosheet hampered FRET, with a consequent strong FL signal. Under the optimal experimental conditions, the FL intensity of the sensing system showed a good linear correlation with ZEN concentration in the range of 1.5-10.0 ng mL-1, and a detection limit (S/N = 3) of 0.68 ng mL-1. The sensing system delivered enhanced specificity for the detection of ZEN, and can find wide application in the detection of other toxins by replacing the sequence of the recognition aptamer.

Colloidal Au sphere and nanoflower-based immunochromatographic strips for sensitive detection of zearalenone in cereals.

Zearalenone (ZEN), also known as an F-2 toxin, is a secondary metabolite in the toxic Fusarium species with estrogen properties. ZEN and its derivatives can cause developmental and reproductive disorders in humans and other mammals. In this study, colloidal Au spheres (AuSPs) and Au nanoflowers (AuNFs) were used as signal labels to detect ZEN in cereals, and the critical factors affecting the sensitivity of the immunochromatographic strip (ICS), namely the volume of antigen, antibody, and probe quantities were optimized and compared in detail. Since the large specific surface area of AuNFs reduces the steric hindrance of proteins, it is more conducive to improving the fixation rate of antibodies and proteins. Compared with the traditional colloidal AuSP immunochromatographic strip (AuSP-ICS), the volume of the antibody used in the AuNF immunochromatographic strip (AuNF-ICS) was 0.6 times that in the AuSPs-ICS. At the same antigen volume, a lower amount of probe can achieve the desired visual detection effect and higher sensitivity. For the AuNF-ICS, the limit of detection (LOD) was as low as 0.08 ng mL-1. ZEN could be detected quickly and accurately from 0.08-10.2 ng mL-1. And the AuNF-ICS had a high degree of specificity and sensitivity to ZEN. In summary, the AuNF-ICS serves as a valuable tool in large-scale on-site detection of ZEN.

A Methylene Blue and Ag+ Ratiometric Electrochemical Aptasensor Based on Au@Pt/Fe-N-C Signal Amplification Strategy for Zearalenone Detection

A Methylene Blue and Ag+ Ratiometric Electrochemical Aptasensor Based on Au@Pt/Fe-N-C Signal Amplification Strategy for Zearalenone Detection

MoS2 quantum dots and titanium carbide co-modified carbon nanotube heterostructure as electrode for highly sensitive detection of zearalenone.

Anovel electrochemical sensor has been fabricated for sensitive determination of zearalenone (ZEA) in food samples based on molybdenum disulfide quantum dots (MoS2 QDs) and two-dimensional titanium carbide (2D-Ti3C2Tx MXene) co-modified multi-walled carbon nanotube (MWCNT) heterostructure. Physical and electrochemical characterizations reveal that 2D-Ti3C2Tx and MoS2 QDs co-modified MWCNTs yields synergistic signal amplification effect, together with large specific surface area and excellent conductivity for the heterostructure, endowing the developed sensor with high detection performance to ZEA. Under optimized conditions, the sensor shows a wide linear range from 3.00 to 300ngmL-1 and a low limit of detection (LOD) of 0.32ngmL-1, which is far lower than the maximum residue limits (MRLs) settled by the European Commission. In addition, it exhibits excellent selectivity, high reproducibility with a relative standard deviation (RSD) of 1.1%, and good repeatability (RSD 1.1%). In real sample analysis recoveries ranged from 94.8 to 105% showing the proposed electrochemical sensor has high potential in practical applications. This work presents an effective and valuable pathway for the useof novel heterostructure in the biosensing field.

Development of Indirect Competitive ELISA and Visualized Multicolor ELISA Based on Gold Nanorods Growth for the Determination of Zearalenone.

In this study, a zearalenone (ZEN) hapten was designed and prepared against the mycotoxin ZEN, and the original coating ZEN-ovalbumin (ZEN-OVA) was prepared by conjugation with OVA. Based on the gold nanorods (AuNRs) of uniform size and stable properties synthesized by the seed-mediated method, the indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and the AuNRs growth-based multicolor ELISA for detecting ZEN toxin were further established. Under the optimal experimental conditions, the coating amount of ZEN-OVA: 0.025 μg/well, antibody (Ab) dilution factor: 32,000 times, blocking solution: 0.5% skimmed milk powder, enzyme-labeled secondary Ab diluted 10,000 times, and a pH of the PBS buffer at 7.4, the sensitivity (IC50) of the established ic-ELISA for ZEN detection reached 0.85 ± 0.04 μg/L, and the limit of detection (IC15) reached 0.22 ± 0.08 μg/L. In the multicolor ELISA based on the growth of AuNRs, as the content of ZEN increased, the mixed solution exhibited a significant color change from brownish red to colorless. ZEN concentration as low as 0.1 μg/L can be detected by the naked eye (brown red to dark gray). This study provided an effective analysis strategy for the rapid screening and accurate monitoring of the ZEN contaminant in foods.

Development of a label-free plasmonic gold nanoparticles aggregates sensor on the basis of charge neutralization for the detection of zearalenone

Mycotoxin contamination of corn has been considered a serious problem because it can accumulate in different organs or tissues via ingestion or skin contact and cause several health problems in humans. We have constructed a label-free, colorimetric, and fluorescence dual-channel sensing platform for the detection of zearalenone. Here, we demonstrate that plasmonic gold nanoparticles aggregates could be rapidly formed on the basis of charge neutralization by positively charged SYBR Green I. The sensing platform allowed quantitative detection as low as 0.89 μg kg−1 and visual detection as low as 2.5 μg kg−1. The charge neutralization strategy eliminates a major source of instability in conventional gold nanoparticles colorimetric measurements and paves the way for accurate, label-free bioanalysis.

Copper Oxide Nanoparticle-Based Immunosensor for Zearalenone Analysis by Combining Automated Sample Pre-Processing and High-Throughput Terminal Detection.

A rapid and high-throughput fluorescence detection method for zearalenone (ZEN) based on a CuO nanoparticle (NP)-assisted signal amplification immunosensor was developed using an automated sample pretreatment and signal conversion system. CuO NPs with high stability and biocompatibility were used as carriers to immobilize anti-ZEN antibodies. The obtained CuO NP-anti-ZEN can maintain the ability to recognize target toxins and act as both a signal source and carrier to achieve signal conversion using automated equipment. In this process, target toxin detection is indirectly transformed to Cu2+ detection because of the large number of Cu2+ ions released from CuO NPs under acidic conditions. Finally, a simple and high-throughput fluorescence assay based on a fluorescent tripeptide molecule was employed to detect Cu2+, using a multifunctional microporous plate detector. A good linear relationship was observed between the fluorescence signal and the logarithm of ZEN concentration in the range of 16.0–1600.0 μg/kg. Additionally, excellent accuracy with a high recovery yield of 99.2–104.9% was obtained, which was concordant with the results obtained from LC-MS/MS of naturally contaminated samples. The CuO NP-based assay is a powerful and efficient screening tool for ZEN detection and can easily be modified to detect other mycotoxins.

A label-free electrochemical immunosensing platform based on PEI-rGO/Pt@Au NRs for rapid and sensitive detection of zearalenone

In this work, we design an immunosensor for zearalenone (ZEN) detection with PEI-rGO/Pt@Au NRs nanocomposite as the modification material. PEI-rGO/Pt@Au NRs nanocomposite have good stability, conductivity and a large specific surface area, so they are chosen as the substrate material for the modified electrode, which is beneficial in improving the detection performance of the sensor. When antibody binds to ZEN, the current signal decreases, and the response signal changes after ZEN incubation, recorded by differential pulse voltammetry (DPV) methods. Under the optimised conditions, the electrochemical response of the constructed immunosensor shows a linear relation to a wide concentration range from 1 pg/mL to 1 × 106 pg/mL with a detection limit of 0.02 pg/mL. Additionally, the proposed electrochemical immunosensor has high selectivity, good stability and great potential for the trace detection of ZEN in real samples.

One-step time-resolved fluorescence microsphere immunochromatographic test strip for quantitative and simultaneous detection of DON and ZEN.

Deoxynivalenol (DON) and zearalenone (ZEN) are mycotoxins that contaminate a wide range of grains and crops. In this study, a one-step time-resolved single-channel immunochromatographic test strip based on europium ion polystyrene fluorescence microspheres was first developed for sensitive and quantitative detection of DON and ZEN. The concentration of the artificial antigen and the mass ratio of the monoclonal antibody to fluorescent microspheres for conjugation were optimized to simplify the sample addition process during immunochromatographic assay and improve the on-site detection efficiency. The limits of detection (LOD) of the single-channel immunochromatographic test strip for DON and ZEN detection were 0.17 and 0.54μg/L, respectively. Meanwhile, the dual-channel immunochromatographic test strip was designed to simultaneously detect DON and ZEN, with LODs of 0.24 and 0.69μg/L achieved for DON and ZEN, respectively. The developed test strips also yielded recovery results consistent with that obtained by LC-MS/MS for DON and ZEN detection in real samples of wheat and corn flour, confirming the practicability and reliability of the test strip. The developed immunochromatographic test strips realize quick and sensitive detection of DON and ZEN, exhibiting potential for broad applications in the point-of-care testing platform of multiple mycotoxins in agricultural products. Graphic abstract.

Highly sensitive aptasensor based on ‘rose petal’ shaped iron nanoparticles decorated on 3D graphene for detection of zearalenone

Zearalenone (ZEA), a mycotoxin mainly synthesized by Fusarium graminearum and F. culmorum is a widespread contaminant of several important crops such as wheat, maize, and paddy causing major plant diseases. Environmental factors such as rain and wind aids in the dispersal of ZEA in the soil and waters which affects aquatic lifes. ZEA causes detrimental health effects such as hyper-oestrogenism and premature abortions to human and animals when contaminated crops are ingested. Hence, it is vital to detect ZEA as early precautionary step in lowering the risks related with the health impairment to human and animals, as well as environmental contamination. Conventional methods are time-consuming and complex, thus, this study aimed on developing a highly sensitive biosensor using graphene-nickel decorated with ‘rose petal’ shaped iron nanoflowers (GNINF) as the transducer and aptamer as the bioreceptor. Low-pressure chemical vapour deposition is used to grown 3D-graphene followed by electrochemical deposition of iron (II) sulphate on its surface to form iron nanoflowers. Immobilisation of chemical and biomolecules were done using the layer-by-layer technique. Field-Emission Scanning Electron Microscopy showed prominent ‘rose petal’ shaped nanoflowers on the graphene surface. This unique assembly creates large surface area for immobilisation and better electric charge transfer on the material surface. The existence of hydroxyl group on the surface of GNINF also plays a role as linker to the surface. Besides, the sensitivity of the aptasensor was characterised using electrochemical impedance spectroscopy. The limit of detection achieved in this study is 1 fg ml−1 and the linear range is 1 fg ml−1 to 1 ng ml−1, which is highly sensitive than most reported biosensors. Overall, this highly sensitive aptasensor is a straightforward and cheap alternative for detecting ZEA in crops and the environment.

Surface-enhanced Raman spectroscopy aptasensor for simultaneous determination of ochratoxin A and zearalenone using Au@Ag core-shell nanoparticles and gold nanorods.

Thedesign and fabrication of a surface-enhanced Raman scattering (SERS) aptasensor forsimultaneous detection of zearalenone (ZEN) and ochratoxin A (OTA) in wheat and corn samplesis described. The capture and reporter probes were SH-cDNA-modified gold nanorods and SH-Apt-modified Au@Ag core-shell nanoparticles, respectively. After recognizing OTA and ZEN aptamers and complementary strands (SH-cDNA), the reporter probe generated a strong SERS signal. The preferred binding of OTA and ZEN aptamers to OTA and ZEN, respectively, caused reporter probes to release the capture probes, resulting in a linear decrease in SERS intensity. The detection of OTA showed good linearity with an R2 value of 0.986, which could be maintained across a wide concentration range (0.01 to 100 ng/mL), with the limit of detection of 0.018 ng/mL. Fordetection of ZEN, good linearity with an R2 value of 0.987 could be maintained across a wide concentration range (0.05 to 500 ng/mL), with 0.054 ng/mL as the limit of detection. Goodaccuracy (relative standard deviation < 4.2%) during mycotoxin determination as well as excellent quantitative recoveries (96.0-110.7%) during the analysis of spiked real samples was achieved. The proposed SERS aptasensor exhibited excellent performance in the detection of OTA and ZEN in real food samples. Hence, by simply changing the aptamer, this new model can be applied tothe detection of multiple mycotoxins in the food industry.

Multifunctional bacteria-derived tags for advancing immunoassay analytical performance with dual-channel switching and antibodies bioactivity sustaining

Constructing multifunctional immunochromatographic assays (ICA) carriers with multiple signals and retaining bioactivity of monoclonal antibodies (mAbs) are conducive to the sensitive and accurate point-of-care testing (POCT). To fulfill this pressing need, a microorganism-based microsphere mediated dual-modal ICA (DICA) was developed for sensitive and reliable detection of zearalenone (ZEN). As the key to the biosensor, a superb biotag with an intact coccus morphology was designed based on Staphylococcus aureus biosynthesized quantum dots incorporating Ru(bpy)32+ (SAQDsRu), in which SA offered a specific recognition capacity for Fc region of mAbs, QDs endowed a naked-eye discernible colorimetric signal on the SA, and robust fluorescence signal that remedied for the insufficient brightness of QDs was derived from Ru(bpy)32+. The characterization of SAQDsRu-labeled mAb (SAQDsRu-mAb) probe demonstrated strong luminescence, excellent stability and high affinity with ZEN (affinity constant was approximately 1.723 × 109 M−1), which can significantly improve the detection sensitivity. Impressively, a portable sensing system was developed by the integration of SAQDsRu-DICA with a smartphone-based readout. After optimization, this DICA indicated a limit of detection reaching down to 0.008 ng/mL (colorimetric mode) and 0.0058 ng/mL (fluorescent mode), which were much lower than that of conventional gold nanoparticles-based ICA (0.1029 ng/mL), possessing favorable specificity and repeatability (relative standard deviation (RSD) < 10%). Moreover, the feasibility of the immunoassay was further assessed by measuring ZEN in real samples with satisfactory recoveries, and the results are good consistent with liquid chromatography tandem mass spectrometry (LC-MS/MS).

Development of Fe3O4@Au nanoparticles coupled to Au@Ag core-shell nanoparticles for the sensitive detection of zearalenone

Agricultural products are frequently contaminated by mycotoxins; thus, the accurate detection of mycotoxins is important to food safety. Zearalenone (ZEN), a mycotoxin produced by certain Fusarium and Gibberella species, is a group III carcinogen. We developed a universal surface-enhanced Raman scattering (SERS) aptasensor for the detection of ZEN. The SERS biosensor consists of two functional nanomaterials: sulfhydryl (SH)-ZEN aptamer complementary DNA-modified Fe3O4@Au was used as a capture probe and SH-ZEN aptamer-modified Au@Ag core-shell nanoparticles served as reporter probes. In the absence of ZEN, the highest Raman signal was obtained owing to the SERS effects of Fe3O4@Au and Au@Ag core-shell nanoparticles. Conversely, the addition of ZEN triggered the release of Au@Ag core-shell nanoparticles from Fe3O4@Au, leading to a decrease in SERS intensity after magnetic separation. Hybridization of the ZEN aptamer and its complementary strand generated a strong SERS signal from the reporter probe. Moreover, preferential binding of the ZEN aptamer to ZEN was observed. The signal intensity in SERS decreased linearly when the capture probes released the reporter. For ZEN detection, a linear range from 0.005 to 500 ng mL−1, with an R2 of 0.9981, was obtained. The detection limit was 0.001 ng mL−1. The SERS aptasensor showed excellent performance for analytical applications with real-world samples (beer and wine). This study presents a new model for the detection of mycotoxins based on simple changes in aptamers.