Detection Of Pesticide Residues Research Articles

An anti-scratch flexible SERS substrate for pesticide residue detection on the surface of fruits and vegetables

We propose an anti-scratch flexible surface-enhanced Raman scattering substrate with arrayed nanocavity microstructures fabricated by colloidal lithography. The nanocavity microstructure of the substrate can well protect the inner gold nanoparticles during wipe sampling. The prepared flexible substrate was able to detect 4-aminothiophenol (4-ATP) with a concentration down to 1 fM. Furthermore, the substrate was used to detect 6-BA residues on the surface of apples and bean sprouts through wipe sampling, which shows great potential in the field of rapid on-site detection, especially in the detection of pesticide residues on the surface of fruits and vegetables.

DETERMINATION OF PESTICIDE RESIDUES IN MUSCLE AND ORGANS OF COW, CAMEL AND GOAT IN BIRNIN KEBBI, KEBBI STATE, NIGERIA

Pesticide residues in muscle and organs of farm animals (cow, camel and goat) were assessed. Samples of muscle (meat), liver, kidney and heart were collected from central abattoirs in the four emirate councils of Kebbi State in order to determine their level of contamination. Residues were extracted from the samples using standard operating procedures and analysed with GC-MS (Intuvo 9000 model) coupled with ECD. Total pesticide residues in the samples ranged from 0.113 to 2.532µg/Kg (muscle), 0.095 to 2.341µg/Kg (liver), 0.047 to 0.931µg/Kg (kidney and 0.026 to 0.361µg/Kg (heart). Pesticide residues concentrations were in the order; muscle > liver > kidney > heart. Analysis of the results obtained also shown that 12.150% of samples from Yauri emirate were contaminated with pesticide residues, 11.249% were contaminated from Argungu, 7.834% from Zuru and 5.784% from B/Kebbi. Samples of muscle and organs from cow accumulated 47.04% of the detected pesticide residues, Camel accumulated 44.48% of the detected residues and goat muscles and organs accumulated 8.48% of the detected pesticide residues. The concentrations of all the assessed pesticides observed in the muscle and organs were however lower than the recommended maximum residual limits (MRLs).

Fluorescent aptamer-modified mesoporous silica nanoparticles for quantitative acetamiprid detection.

Acetamiprid (ACE) is widely used to control aphids, brown planthoppers, and other pests in agricultural production. However, ACE is difficult to degrade in the environment, resulting in excessive residue, which causes acute and chronic toxicity to human beings and non-target organisms. Therefore, the development of a rapid, convenient, and highly sensitive method to quantify ACE is essential. In this study, aminated mesoporous silica nanoparticles (MSNs-NH2) were synthesized by one-pot method, and 6-carboxyl fluorescein modified aptamers (FAM-Apt) of ACE were adsorbed on the surface of MSNs-NH2 by electrostatic interaction. Finally, a simple and sensitive fluorescence analysis method for the rapid detection of ACE was established. In the absence of ACE, the negatively charged FAM-Apt was electrostatically bound to the positively charged MSNs-NH2, followed by centrifugation to precipitate MSNs-NH2@FAM-Apt, and no fluorescent signal was detected in the supernatant. In the presence of ACE, the specific combination of FAM-Apt with ACE was greater than its electrostatic interaction with MSNs-NH2, so that FAM-Apt was separated from MSNs-NH2, and the supernatant had strong fluorescence signal after centrifugation. For ACE detection, the linear concentration range was 50-1100ng/mL, and the detection limit (LOD) was 30.26ng/mL. The method exhibited high sensitivity, selectivity and reproducibility, which is suitable for practical sample analysis and provides guidance for rapid detection of pesticide residues.

Aptamer-based sensor for specific recognition of malathion in fruits and vegetables by surface-enhanced Raman spectroscopy and electrochemistry combination

ABSTRACT: Aptamers are single-stranded DNA or RNA that can specifically bind to a target substance with high affinity. Pesticides have obvious biological effects and are very harmful to the human body and the environment. Surface-enhanced Raman spectroscopy (SERS) can be combined with aptamer for the rapid detection of pesticide residues because of its simple operation and high sensitivity. However, a single analysis method has certain limitations and can't satisfy the research of complex system. In this study, SERS and electrochemical were used to prove the specific recognition of malathion by aptamer. The biochemical sensor directly detected the SERS spectra of the aptamer, malathion, and aptamer-malathion through the SERS method. Malathion could also be verified the specific recognition of aptamer by differential pulse voltammetry (DPV) and cyclic voltammetry (CV). Based on the peak difference before and after the combination of malathion and aptamer, a detection method for the specific recognition of malathion in a complex system was established. The identification mechanism was discussed. Then, the practical detection of malathion demonstrated the practicability of the method. The method was applied for the detection of malathion in spiked apple and bell pepper samples. The recoveries were in the range of 89.80%–106.50%, and the relative standard deviations were from 2.51%–6.92%. The use of aptamers for the specific detection of pesticides will have the potential for broad applications in the future.

DETERMINATION OF PESTICIDE RESIDUES IN MUSCLE AND ORGANS OF COW, CAMEL AND GOAT IN BIRNIN KEBBI, KEBBI STATE, NIGERIA

Pesticide residues in muscle and organs of farm animals (cow, camel and goat) were assessed. Samples of muscle (meat), liver, kidney and heart were collected from central abattoirs in the four emirate councils of Kebbi State. Residues were extracted from the samples using standard operating procedures and analysed with GC-MS (Intuvo 9000 model) coupled with ECD. Total pesticide residues in the samples ranged from 0.113 to 2.532µg/Kg (muscle), 0.095 to 2.341µg/Kg (liver), 0.047 to 0.931µg/Kg (kidney and 0.026 to 0.361µg/Kg (heart). Pesticide residues concentrations were in the order; muscle > liver > kidney > heart. Analysis of the results obtained also shown that 12.150% of samples from Yauri emirate were contaminated with pesticide residues, 11.249% were contaminated from Argungu, 7.834% from Zuru and 5.784% from B/Kebbi. Samples of muscle and organs from cow accumulated 47.04% of the detected pesticide residues, Camel accumulated 44.48% of the detected residues and goat muscles and organs accumulated 8.48% of the detected pesticide residues. The concentrations of all the assessed pesticides observed in the muscle and organs were however lower than the recommended maximum residual limits (MRLs).

Sowing kernels for food safety: Importance of rapid on‐site detction of pesticide residues in agricultural foods

AbstractSustainable cultivation and safe agricultural production are needed to ensure the safety of all living organisms and ecosystems. Most agricultural foods currently contain unacceptable levels of pesticide residues. In an attempt to ensure pest control and high crop yields, farmers spray a large number of pesticides in quantities that exceed the safety limits for agricultural crops. Pesticide residues are highly toxic to humans, causing severe and even deadly diseases. Traditional analytical strategies for laboratory pesticide detection are often limited because they are time‐consuming and require trained personnel, which makes them very costly. Hence, accurate, rapid, and on‐site analysis of pesticides is drawing increasing attention for food safety reasons. Therefore, on‐site or point‐of‐care (POC) detection of pesticide residues has become an approach of paramount importance. Recently, various portable detection technologies, such as colorimetric, fluorescence, electrochemical, surface plasmon resonance, chemiluminescence, phosphorescence, microfluidic, and surface‐enhanced Raman scattering techniques, have been designed for on‐site monitoring of pesticide residues in vegetables and fruits. These devices have demonstrated great power of detection in preclinical agricultural settings. This review highlights the emerging insights and novel advances in portable devices, POC technologies, and on‐site sensing approaches for the detection of pesticide residues in agricultural foods. However, greater rigor in the design of portable pesticide‐detection devices is essential. The increasing demand for rapid detection of hazardous pesticide components has prompted a timely opportunity to summarize recent developments and progress in portable devices and on‐site sensing strategies to evaluate food safety.

Effective adsorption and in-situ SERS detection of multi-target pesticides on fruits and vegetables using bead-string like Ag NWs@ZIF-8 core-shell nanochains

In-situ, real-time and sensitive detection of multiple pesticide residues in food is always an important issue in food safety. Herein, a novel multifunctional bead-string like Ag nanowires@zeolitic imidazolate framework-8 (Ag NW@ZIF-8) core–shell nanochains was successfully synthesized and utilized as surface-enhanced Raman scattering (SERS) substrate for in-situ and simultaneous detection of pesticide residues. Due to the microporous framework structure of ZIF-8 shell and the plasmon property of Ag NWs core, the Ag NWs@ZIF-8 composite was demonstrated to have strong adsorption performance and high SERS activity (enhancement factor: 4.6 × 107). The Ag NWs@ZIF-8 based SERS strategy achieved sensitive detection of two water-insoluble pesticides methylparathion and carbaryl with detection limits as low as 7.6 × 10−9 M and 5.7 × 10−9 M, as well as the in-situ detection of multi-pesticide residues on fruits and vegetables with acceptable recovery ranged from 77.4 % to 117.5 %, demonstrating its great potential for in-situ and rapid detection of food contaminants.

Evaluation of Pesticide Residues Occurrence in Random Samples of Organic Fruits and Vegetables Marketed in Poland.

In recent years, organic food, produced with the use of natural means and production methods, has been gaining more and more popularity among consumers. This is due, inter alia, to their belief that it is more abundant in health-promoting bioactive compounds and safer than conventional food. Consumers are increasingly aware of the harmfulness of plant protection products used in intensive agriculture, which are not allowed in organic production. At the same time, it is reported that a certain share of organic products on the EU market are contaminated with pesticide residues, which may raise consumer concerns and lead to a loss of trust in organic food. The aim of the present study was to investigate the problem of pesticide residues occurrence in random samples of organically produced fruits and vegetables (apples, potatoes, carrots, and beetroots) commonly used in the Polish households, and which are available directly from the organic producers in open markets in Poland. For simultaneous analysis of 375 pesticides, an LC-MS/MS system consisting of an Eksigent expert ultraLC 100-XL coupled to a triple quadrupole mass spectrometer QTRAP 6500 and GC Agilent 6890 N equipped with ECD/NPD system were used. Among the 96 vegetable and fruit samples studied, 89 samples (92.7%) were free from detectable pesticide residues, 7 samples (7.3%) of carrot (5) and potato (2) were contaminated, and in 1 of them (1.0%) the detected residues exceeded the maximum residue limit (MRL). None of the tested apple and beetroot samples were found to contain detectable residues. These findings are important for Polish consumers who look for high-quality organic food. However, the presence of detectable residues in a small proportion of the organic samples indicates a need to strengthen the monitoring of pesticides in organic crops, to educate farmers and to raise their awareness regarding the risks of unauthorized use of pesticides banned in organic farming, which can damage the reputation of the whole organic sector.

Detection of Pesticide Residues in The Canal Irrigation System of The Upper Mekong Delta, Cambodia

Pesticide refers to all chemical compounds which are mainly used in agriculture to control pests and weeds. In many developing countries, the increasing use of pesticides is due to agricultural activities for intensification purposes. The potential consequences for the environment, health and biodiversity are difficult to anticipate, as the presence of pesticides is difficult and rarely monitored. This is the case in the upper Mekong delta in Cambodia where many irrigation systems are being developed. The aim of the study is to provide a preliminary assessment of the situation by looking for the presence of pesticides in the water within a representative irrigation system. Water samples were collected in canals, river, irrigated rice field and groundwater in both dry and wet seasons. Solid-phase extraction (SPE) and gas chromatography-mass spectrometry (GC-MS) methods were used to purify and analyze the samples. Among 37 pesticides used by farmers and detectable, 6 were found in water. Paclobutrazol and propiconazole are moderately hazardous, hexaconazole is slightly hazardous, fluquinconazole presents acute toxicity and azoxystrobin is unlikely to present acute hazard. Hexaconazole and paclobutrazol are classified as highly persistent in water, while others are slightly to moderately persistent. Pesticides were found mostly in groundwater but they were found in the canals and rice field only in dry season. This means that pesticides may accumulate in the aquifer while the flooding has a flushing effect on pesticides. Further analyses should be extended to a bigger scale and be continued monitoring.

Development of Molecularly Imprinted Polymers for Fenthion Detection in Food and Soil Samples.

Modern agricultural production is greatly dependent on pesticide usage, which results in severe environmental pollution, health risks and degraded food quality and safety. Molecularly imprinted polymers are one of the most prominent approaches for the detection of pesticide residues in food and environmental samples. In this research, we prepared molecularly imprinted polymers for fenthion detection by using beta-cyclodextrin as a functional monomer and a room-temperature ionic liquid as a cosolvent. The characterization of the developed polymers was carried out. The polymers synthesized by using the room-temperature ionic liquid as the cosolvent had a good adsorption efficiency of 26.85 mg g−1, with a short adsorption equilibrium time of 20 min, and the results fitted well with the Langmuir isotherm model and pseudo-second-order kinetic model. The polymer showed cross-selectivity for methyl-parathion, but it had a higher selectivity as compared to acetamiprid and abamectin. A recovery of 87.44–101.25% with a limit of detection of 0.04 mg L−1 and a relative standard deviation of below 3% was achieved from soil, lettuce and grape samples, within the linear range of 0.02–3.0 mg L−1, using high-performance liquid chromatography with an ultraviolet detector. Based on the results, we propose a new, convenient and practical analytical method for fenthion detection in real samples using improved imprinted polymers with room-temperature ionic liquid.

Isotopes Don’t Lie, differentiating organic from conventional banana (Musa AAA, Cavendish subgroup) fruits using C and N stable isotopes

With the dramatic increase of organic banana production worldwide, it is essential to be able to monitor compliance with organic specifications. While the detection of pesticide fraud is routinely controlled by detecting pesticide residues in organic bananas, the detection of fertilizer fraud is much more complex. We compared the δ13C and δ15N isotopic values of green bananas from organic and conventional farms at seven sites around the world. In our whole dataset, the δ15N values of banana fruits ranged between −1.25 and + 8.91‰. In all sites, δ15N values of organic banana were significantly higher than conventional fruits (mean value of + 5.24‰ and + 2.342‰, respectively). Conversely, the type of fertilization did not significantly alter δ13C values. Our results suggest that it is possible, upon arrival in importing countries, to differentiate bananas grown with synthetic fertilizer from those grown with organic fertilizer.

Dye-Encapsulated Lanthanide-Based Metal-Organic Frameworks as a Dual-Emission Sensitization Platform for Alachlor Sensing.

As an important factor affecting global agricultural output, pesticides have a significant impact on the ecosystem. It is an urgent task to accurately and conveniently detect pesticide residues after their application. Herein, a fluorescent dye@MOF platform was designed via the encapsulation of rhodamine B (RhB) into the MOF structure (named RhB@HNU-48), which can significantly enhance the sensing sensitivity of alachlor with an ultralow detection limit of 0.59 ppb. The improved sensitivity of RhB@HNU-48 to pesticides was attributed to the host-guest interactions that affect the excitation and emission spectra of the composites. Based on the sensing capability of RhB@HNU-48, a logic gate was built to evaluate the safety level of alachlor residues in rivers and soil. The preparation of photofunctionalized MOF composites through modulation of host-guest interactions offers a promising strategy for the construction of desired sensors for agricultural residues.

Detection of pesticide residues and risk assessment from the local fruits and vegetables in Incheon, Korea

This study was conducted to investigate the pesticide residue concentrations and assess potential human health risks from fruit and vegetable consumption in Incheon. A total of 1,146 samples of 20 different types of fruits and vegetables were collected from the Incheon area in 2020. The pesticide residues were analyzed by the multi-residue method of the Korean Food Code for 400 different pesticides. Among the fruit and vegetable samples, 1,055 samples (92.1%) were free from detectable residues, while 91 samples (7.9%) contained residues and 11 samples (1.0%) had residues exceeding the Korean maximum residue limit. A total of 32 different pesticide residues were found and 8 residues exceeded MRLs. The most frequently detected pesticide residues were chlorfenapyr, procymidone, etofenprox, pendimethalin, fluopyram and azoxystrobin. The highest values of short term and long term exposure were obtained in the case of consumption of lettuce(leaves) with chlorfenpyr. For chronic dietary exposure, the cumulative hazard index (cHI) were below 100%. The results of this study showed that the detected pesticides were not exposed to potential health risks through the consumption of fruits and vegetables.

Detection of Pesticide Residues in Olive Leaves From İzmir, Turkey

Turkey, especially the Aegean region, has very suitable lands for olive cultivation. In this work, the olive leaves extracts were treated with pesticide solution and also Bordeaux mixture. Olive varieties in their natural environment were collected after 1 year, and the amount of pesticides they contained was measured. As a result of the QuEChERS method using LC-MS/MS and GC-MS/MS techniques, no pesticide residues were detected in any samples. The pesticide residues determined in the samples used in this study do not exceed the maximum limits specified in the Turkish Food Codex and the European Commission. There are no quantifiable residues of abamectin, acetamiprid, azoxystrobin, difenoconazole, diflubenzuron, diflufenican, dimethoate, dodine, emamectin benzoate, indoxacarb, lufenuron, malathion, novaluron, phosmet, pyriproxyfen, spinosad, thiacloprid, and triflumuron were detected in any olive samples by using LC-MS/MS method. Also, alpha-cypermethrin, beta-cyfluthrin, cyflutrin, deltamethrin, and lambda-cyhalothrin were not detected in samples by GC-MS/MS method. Accordingly, they agree with the results found. The results obtained showed that the pesticide solution used protected the olives from pests and did not have a harmful effect on the olives.

Modern Analytical Methods for the Analysis of Pesticides in Grapes: A Review.

Currently, research on the determination of pesticides in food products is very popular. Information obtained from research conducted so far mainly concerns the development of a methodology to determine the content of pesticides in food products. However, they do not describe the content of the pesticide used in viticulture in the resulting product. Over the past decade, this study has examined analytical methodologies for assessing pesticide residues in grapes. Scopus, Web of Science, Science Direct, PubMed, and Springer databases were searched for relevant publications. The phrases “pesticides” and “grapes” and their combinations were used to search for articles. The titles and annotations of the extracted articles have been read and studied to ensure that they meet the review criteria. The selected articles were used to compile a systematic review based on scientific research and reliable sources. The need to study the detection of pesticide residues in grapes using advanced analytical methods is confirmed by our systematic review. This review also highlights modern methods of sample preparation, such as QuEChERS, SPME, PLE, dLLME, and ADLL-ME, as well as the most used methods of separation and identification of pesticides in grapes. An overview of the countries where residual grape pesticide amounts are most studied is presented, along with the data on commonly used pesticides to control pests and diseases in grape cultivation. Finally, future possibilities and trends in the analysis of pesticide residues in grapes are discussed by various analytical methods.

Alterations in reproductive hormone levels among farm women and their children occupationally exposed to organophosphate pesticides

ABSTRACT Exposure to organophosphorus pesticides may lead to reproductive hormone dysfunction. Even among children of pubertal age, the exposure may disrupt growth, development, and maturation. The present study was conducted to assess the alterations in the reproductive hormone levels, among farm women (24–45 years, n = 129) and their children (9–12 years, n = 66 and 13–15 years, n = 63) and compare them with age and gender-matched control group [women (n = 134) and their children (9–12 years, n = 69 and 13–15 years, n = 65)] belonging to villages of Ranga Reddy District, Telangana, India. Blood pesticide residues and reproductive hormone (follicle-stimulating hormone-FSH, luteinizing hormone-LH, estradiol, and testosterone) levels were analyzed. The detected pesticide residues (ng/mL) were chlorpyrifos, diazinon, malathion, and monocrotophos among the farm women, while the farm children of 9–12 years age groups were detected with residues of chlorpyrifos, diazinon, malathion, monocrotophos, and phosalone. The farm children of 13–15 years age group were detected with residues of chlorpyrifos, diazinon, malathion, monocrotophos, and phosalone. However, no residues were detected among the samples of women and children of control groups. Significantly lower levels of FSH (in follicular phase) were observed among the farm women than the control group. Significant alterations in FSH and LH levels of farm women were observed with a significant correlation between the chlorpyrifos residue levels and estradiol hormone. While no such significant change in hormone levels was observed among the farm children of both age groups of both genders. Though the present study showed pesticide-induced alterations in hormone levels among the farm women, research is needed to elucidate the critical windows during which exposure may adversely affect the reproductive system in children at the pubertal stage and women at reproductive age and subsequently their progeny’s health at a later stage of life.

On-site rapid and simultaneous detection of acetamiprid and fipronil using a dual-fluorescence lab-on-fiber biosensor

Adual-fluorescence lab-on-fiber biosensor was developed for the rapid and simultaneous on-site determination of acetamiprid and fipronil, based on time-resolved effect and indirect competitive immunoassay principle. The optical fiber modified with two hapten-protein conjugates servesas a bifunctional bio-probe. The dual-color fluorescent reporters were prepared via labeling acetamiprid and fipronil antibodies with Cy5.5 and Alexa Fluor 555, which were excited at 635-nm and 520-nm laserwavelengths, respectively. In the presence of targets, the binding sites of corresponding antibodies were occupied and less antibodies were connected to the probe surface, resulting in the reduction of fluorescence signal. The concentration of acetamiprid and fipronil was determined by measuring the fluorescence signals at 568nm and 702nm (emission wavelengths), respectively. Under optimal conditions, the linear response range was 14.2-225.4ng/L for acetamiprid and 25.1-162.8ng/L for fipronil, and the limit of detection was 6.51ng/L and 17.8ng/L for acetamiprid and fipronil, respectively. The method was successfully applied to the simultaneous detection of acetamiprid and fipronil in three environmental samples, and the recoveries were between 90 and 128%. The dual-fluorescence lab-on-fiber biosensor provides a feasible platform for simultaneous and rapid detection of multiple pesticide residues. A dual-fluorescence lab-on-fiber biosensor was developed for the rapid and simultaneous on-site determination of acetamiprid and fipronil. A bifunctional bio-probe was prepared from the optical fiber modified with two hapten-protein conjugates. Acetamiprid and fipronil antibodies were labeled with different fluorophores and used as dual-color fluorescent reporters.

Highly selective and sensitive colorimetric detection for glyphosate based on β-CD@DNA-CuNCs enzyme mimics

A colorimetric assay based on an enzyme-inhibition strategy is promising for the on-site detection of pesticide residues. However, very few works of pesticide detection were reported based on the inhibition toward nanozymes although nanozymes have demonstrated many advantages in sensing various targets. Herein, a facile colorimetric detection for Glyp was developed based on β-CD@DNA-CuNCs enzyme mimics. The β-CD@DNA-CuNCs with high peroxidase-like activity was synthesized using random DNA double strands as template and β-CD as surface ligand. β-CD@DNA-CuNCs could catalyze the H2O2-3,3′,5,5′-tetramethylbenzidine (TMB) system. The oxidation product OxTMB with a blue color and presented a large absorption signal at 652 nm. However, Glyp could destroy the synergic effect between redox doublet (Cu2+/Cu+) on the β-CD@DNA-CuNCs surface, resulting in the inhibition of the peroxidase-like activity. Colorimetric detection for Glyp could be established by detecting the changes of absorption signal at 652 nm. The linear range was 0.02–2 μg/mL and the detection limit was 0.85 ng/mL (3δ/s). The method was applied in measuring Glyp spiked in lake water and various food samples. This method had rapidness, high sensitivity, and selectivity advantages, indicating the high application potential in monitoring Glyp residue in food.

RAPID ELECTROCHEMICAL DETECTION OF CARBENDAZIM IN VEGETABLES BASED ON CARBOXYL FUNCTIONALIZED MULTI-WALLED CARBON NANOTUBES

At present, the commonly used methods for the detection of benzimidazole fungicides include high performance liquid chromatography (HPLC), liquid chromatography-mass spectrometry (LC-MS), fluorescence spectrometry and so on. These methods have high sensitivity and accurate results, but they have disadvantages such as complicated pretreatment, long time consuming, expensive equipment and professional operators. Electrochemical sensor detection method has the advantages of high sensitivity, simple operation, low cost and easy on-site inspection, etc., which has attracted extensive attention in the field of pesticide residue detection and analysis. In order to realize the rapid detection of carbendazim content in vegetables, an electrochemical rapid detection method was established by using Carboxyl Functionalized Multi-Walled Carbon Nanotubes (MWCNTs-COOH) modified glassy carbon electrode. In this study, MWCNTs-COOH with special functional groups and large specific surface area were used to modify electrodes to improve the adsorption and enrichment of CBZ on the electrode and amplify the electrochemical signal, aiming to establish a highly sensitive electrochemical rapid detection technology for CBZ. The results showed that: the modified electrode functionalized with MWCNTs-COOH could significantly improve the electron transfer rate on the electrode surface, which made the detection sensitivity of carbendazim higher. The linear range of detection was 0,3 μM~20 μM, and the detection limit was determined as low as 0,06 μM. In this study, MWCNT-COOH with better conductivity, adsorption and stability was used to modify electrode, and constructed the MWCNT-COOH/GCE, which improved the adsorption and accumulation of CBZ, effectively promoted the electron transfer on the surface of the electrode, accelerated the response speed of the electrode and improved the current response, to realize the rapid and sensitive detection of trace CBZ in vegetables. This method had high sensitivity, good anti-interference, and detection stability. It was of great significance to detect carbendazim in vegetables.

Rapid Trace Detection of Pesticide Residues on Tomato by Surface-Enhanced Raman Spectroscopy and Flexible Tapes

The non-judicious use of pesticides in agro-food poses a severe threat to food safety and human health. As an emerging chromatographic fingerprint provider, surface-enhanced Raman spectroscopy analysis (SERS) sheds bright light on sensitive and nondestructive detection of pesticide residues. This research proposed a novel strategy to detect three-pesticide residues (thiabendazole, carbendazim, and chlorpyrifos) on tomato peel based on the flexible and sticky SERS substrate. After selecting the best commercial adhesive tape (3M9080), the SERS substrate was constructed by optimizing the parameters in the preparation process of AuNPs. Therefore, a new simple “tape-wrapped SERS” way for pesticide residue analysis was established with a simple procedure of “absorption, separation, and drop addition.” Based on chemometrics method, the limit of semiquantitative detection was 20, 36, and 80 ng/cm2 for thiabendazole, carbendazim, and chlorpyrifos, respectively, on tomato surface, which indicated that the proposed method could meet the requirement of actual application with a large prospect in agro-food safety detection.