Background: It is well-known that deciency or over exposure to various elements has noticeable effects on human health. The effect of an element is determined by several characteristics, including absorption, metabolism, and degree of interaction with physiological processes. Iron is an essential element for almost all living organisms as it participates in a wide variety of metabolic processes, including oxygen transport, deoxyribonucleic acid (DNA) synthesis, and electron transport. The Study was Conducted Material and Methods: in department of Biochemistry Guru Gobind Singh Medical College Faridkot. Ethical clearance was taken from institutional ethical committee. Proper informed consent was taken from all the participitants. Out of 60 healthy patients, 30 were males and 30 females. The Correlation between iron in three time Result: intervals on the same day (Morning – Evening and Morning – Afternoon )for morning – afternoon values, the p value was <0.001 and the r value was 0.920. For morning – evening, the p value was <0.001 and r value was 0.928, which shows statistically highly signicant correlation. Conclusion: It was conducted that Iron levels showed signicant diurnal variations on same day samples. The variation was statistically non signicant for TIBC. Also no consistant pattern has observed for iron variation. So the time of day for Iron &TIBC estimation does not hold much value and only a fasting sample is required.