Abstract A major challenge in wastewater treatment system design is optimizing the nitrification process in activated sludge reactors, especially in the seafood industry with high concentration of organic matter, fat, oil, and nitrogen. However, in traditional seafood wastewater treatment systems, nitrogen removal is limited by the ratio C:N:P ≈ 100:5:1. With the experiment of the Sequencing Batch Reactor model, the evaluation of biological nitrification reaction is conducted based on the following parameters: organic loading rate, removing efficiency of ammonia - nitrogen, and nitrification rate. By using pre-treatment seafood processing wastewater with a C/N ratio of 5-6, these parameters were determined through an operating biological reactor with F/M from 0.16 to 0.2g BOD5/g MLVSS.d. In terms of the performance of the model, when optimizing the nitrification process, the efficiency removal reaches 78.3-80.0% for BOD5 and 93.9-94.5% for NH4-N. After that, the nitrification process occurred after 30 minutes of reaction phase, reached its maximum rate after 2 hours with nitrification rate from 0.04 to 0.08 g Nox/g MLVSS.d and then maintained for about 1 hour. Moreover, for an optimal nitrification process, the alkaline consumption of the model should be in the range 7.05-7.18 mg CaCO3/mg N.