Design Of Packaging Systems Research Articles

Three Level Fuzzy Signature-Based Decision Methodology for Packaging System Design

Three Level Fuzzy Signature-Based Decision Methodology for Packaging System Design

Determination of the physical and mechanical properties of a potato (the Agria variety) in order to mechanise the harvesting and post-harvesting operations

Studying the physical and mechanical properties of agricultural products has been the subject of criticism and discussion for many years already and has attracted the attention of many researchers. The physical and mechanical properties of agriculture products are the most important parameters in the design of agricultural machinery sorting systems, transmissions, processing and packaging systems. The potato is one of the most important agricultural products as a food resource. The aim of this research is to investigate the physical properties and mechanical behaviour of the potato due to its importance and the current inadequate information about it. In this research, some of the physical and mechanical properties of the potato were measured in standard conditions. The length, width, thickness, geometric mean diameter and arithmetical diameter, sphericity, surface area, aspect ratio, mass, volume, bulk density and the projected mean area were included in the physical properties. The mechanical properties of the potato were determined by using a universal testing machine (MRT-5; Santam, Germany) with a uniaxial compression test. Then, the mechanical properties were measured with four repetitions. The physical properties of the potato such as length, width, thickness, mass, volume and geometric mean diameter had a direct relationship, while the density had an inverse relation to the size. The result of the mechanical properties of the sample, such as vertical stress, elasticity module, deformation energy, fracture force and deformation were obtained respectively as 0.34 MPa, 3.09 MPa, 892.02 J, 8.80 N and 207.22 mm. The comparison of the potato’s real volume with the standard shapes showed that the potato’s shape is similar to an oval due to the largest determination coefficient (R<sup>2</sup> = 0.86). The results of this research can be used for the design and optimisation of the processing equipment, as well as the transporting, sorting and packing of the potato crop.

Homotopy perturbation method with an auxiliary term for the optimal design of a tangent nonlinear packaging system

The homotopy perturbation method with an auxiliary term is applied to obtain an approximate analytical solution for the tangent nonlinear packaging system. To further raise the accuracy of the solution, this work has expanded the possibility of the energy method for the homotopy perturbation method with an auxiliary term. And then, the amplitude and frequency of the tangent nonlinear packaging system can be calculated. The results show that the solution is in good consistent with the numerical solution solved by the Runge–Kutta method. It can be concluded that the proposed method is suitable for the optimal design of a tangent nonlinear packaging system.

Development of sensor system for real-time measurement of respiration rate of fresh produce

Monitoring respiration as rates of either O2 consumption or CO2 production is an important tool to optimise the design of packaging systems or to maintain optimal conditions inside storage systems for fruit and vegetables. Currently, respiration rates are mostly measured by gas chromatography or headspace gas analysers. Both these methods are manual and, for analysis, draw a considerable amount of gas from the system. Thus, they risk lowering the air pressure inside the storage and packaging volumes. In this study, a compact (free volume of 3.3 L) and modular respirometer based on a respiration measuring sphere (RMS88) was developed, which consisted of one O2 (0–25%) and two CO2 sensors (0–0.5% and 0–5%) for continuous real-time monitoring of gas concentrations and respiration rates of fresh produce. This system was tested under different conditions and respiration rates of strawberries were measured. At first, the system was tested without and with flushing of ambient air (every 6 h at 5 L/min) inside the respirometer at 10 °C. The system was further tested with strawberries stored under modified atmospheric conditions and flushing (every 5 h at 5 L/min) with stepwise reductions of O2 from 21% to 16%, 8%, 4%, 2%, and 1% O2 (balanced by nitrogen). Additionally, the developed system was tested under in-situ conditions for strawberries stored inside respirometer, kept in two separate controlled atmosphere (CA) metal chambers at 10 °C and 20 °C respectively. In addition, the respiratory quotient (RQ) of strawberries was calculated from the respective O2- and CO2-based respiration rates at all storage conditions. The modular respirometer proved to be a useful system for rapid, high accuracy and real-time measurement of respiration rates of fresh produce during time windows of several days.

Effect of emulsifier on rheological, textural and microstructure properties of walnut butter

Walnut butter apart from its delicious taste and flavor is rich in monounsaturated fatty acid content and oil separation is one of the problems faced by walnut butter industry. In this investigation, effect of different level of emulsifying agents (mono-diglyceride and lecithin at 0, 0.5 and 1.5% w/w levels) on the rheological (time-independent and time-dependent), size distribution, texture homogeneity (GLCMs and fractal dimension) and stability of walnut butter have been studied. The results revealed that emulsifiers decreased droplets size, which was more pronounced for 1.5% Lecithin (21.58 µm). The walnut butter showed shear-thinning behaviour (n: 0.18–0.21) and the samples containing 1.5% lecithin had the highest consistency coefficient (370.23 Pa sn), thixotropy value (relative of hysteresis area = 39.31%) and stability (separation after 60 days: 14.45%). Increasing the concentration of lecithin increased η0/η∞ value, but no alteration was observed for monoglyceride samples. This increment of η0/η∞ values indicates that structural modification of walnut butter takes place at higher concentration of lecithin. Also, it was observed that the emulsifiers resulted in more uniform texture. Results could be useful in development of the walnut butter making process and packaging system design.

Effect of Temperature and Relative Humidity on the Reaction Kinetics of an Oxygen Scavenger Based on Gallic Acid.

Gallic acid (GA) is a potential oxygen scavenger for food packaging applications. In this study we investigated the effect of temperature and relative humidity (RH) on the reaction kinetics of an oxygen scavenger consisting of GA and sodium carbonate. The reaction was described by a second-order kinetic law and the reaction rate coefficient k as well as the scavenger capacity n were determined from experimental data using a multiple-run downhill simplex method. Both the rate coefficient and the scavenger capacity increased significantly with higher temperatures. At 21°C it was shown that both the rate coefficient and the scavenger capacity increased significantly with higher RH. However, below 54% RH, there was no detectable reaction. For optimum scavenger performance we therefore recommend GA-based scavengers for packaging of food products with a high water activity stored at room temperature. Prior to application, the packaging materials with GA-based scavengers can be stored at 21°C and 54% RH without losing their scavenger activity. The results of this study provide the basis for the functional design of active packaging systems with GA-based oxygen scavengers.

System in package (SiP) technology: fundamentals, design and applications

PurposeThe purpose of this paper is to demonstrate a novel 3D system-in-package (SiP) approach. This new packaging approach is based on stacked silicon submount technology. As demonstrators, a smart lighting module and a sensor systems were successfully developed by using the fabrication and assembly process described in this paper.Design/methodology/approachThe stacked module consists of multiple layers of silicon submounts which can be designed and fabricated in parallel. The 3D stacking design offers higher silicon efficiency and miniaturized package form factor. This platform consists of silicon submount design and fabrication, module packaging, system assembling and testing and analyzing.FindingsIn this paper, a smart light emitting diode system and sensor system will be described based on stacked silicon submount and 3D SiP technology. The integrated smart lighting module meets the optical requirements of general lighting applications. The developed SiP design is also implemented into the miniaturization of particular matter sensors and gas sensor detection system.Originality/valueSiP has great potential of integrating multiple components into a single compact package, which has potential implementation in intelligent applications.

Thinking and Implementation of Construction Plan of Packaging Engineering

Based on the analysis of the development basis and achievement of packaging engineering in Beijing Institute of Graphic Communication (BIGC), the guiding ideology and goals are defined, the construction plan of packaging engineering in BIGC is put forward. Through the construction of packaging engineering, its characteristics and feature of "the combination of packaging technology and intelligent technology" and "the combination of packaging system design and art design" will be outstanding further, become the leader in the whole china and even in the world.

Building an EcoSystem for User-friendly Design of Advanced System in Package (SiP) Solutions

Abstract The Semiconductor Industry has followed “Moore's Law” for more than five decades and has made remarkable progress by continuously shrinking features sizes. However, meeting today's and future market requirements, such as shorter development times, lower up-front cost, lower unit cost, many more gates and pins in even smaller form-factors, integration of heterogeneous functions (logic, memory, analog, RF, MEMS, sensors, optical and others) and higher performance per Watt is becoming much more challenging. These requirements call for advanced IC packaging technologies to enable continued semiconductor revenue and profit growth. In addition to IC packaging-specific expertise and development resources, very close cooperation with knowledgeable partners across all planning, design and manufacturing steps is needed, to create cost-effective and reliable electronic components, sub-systems, even entire systems in a package. This paper analyzes why and where advanced IC packaging technologies are needed and suggests steps for developing Assembly Design Kits (ADKs) and Reference Design Flows, and other essential elements for building a strong “More than Moore”-focused semiconductor EcoSystem.

Module packaging effects on MEMS airbag sensor performance for automobiles

In this paper, the effects of the packaging module housing of MEMS airbag sensor for signal generation is studied. Conventional airbag sensor packaging module for automobiles is composed of the accelerometer MEMS sensor mounted on a printed circuit board, which is assembled inside a plastic housing. And the entire module is mechanically installed by bolts on the automobile frame structure to capture the acceleration from the structure vibration. In this case, the signal path from the frame to the sensor is complex, and the frame vibration is interacted with the module vibration, which may delay and distort the original signal pattern. To remove housing vibration effects, a new sensor packaging on the flexible circuit board was introduced to attach directly on the frame structure surface using adhesive. For the signal characterization, impact tests were performed for the conventional sensor packaging and the directly attached sensor using an aluminum channel and automobile side frame. Numerical analyses were also carried out to understand the vibration effects of the packaging module and the structure. It was found that the vibration behaviors of the frame structure and the module housing, and the module installation location had significant influence on the impact signal generations. The results indicated that the design of the airbag sensor packaging system for proper airbag explosion requires comprehensive engineering considerations.

An industry‐oriented strategy for the finite element simulation of paperboard creasing and folding

The numerical simulation of paperboard creasing and folding processes is of increasing importance for the design and production of safe and reliable packaging systems. The extreme material anisotropy and the complexity of these processes require however simulation capabilities which are seldom available in commercial codes. Several approaches have been proposed in the literature over the years, in most cases making use of non‐linear material models developed ad hoc for this purpose. These models, some of which are very effective and accurate, are not in general available in commercial codes and are often based on the definition of a large number of parameters. In this paper, the possibility to obtain acceptable, first‐hand simulation results using only features already available in a commercial code is investigated. An advanced continuum constitutive model, recently presented in the literature, has been used as a reference for tuning the model and for assessing its accuracy. It is shown how standard features, usually available in state‐of‐the‐art commercial codes, can be employed to deal with the extreme material anisotropy, obtaining qualitatively good results in both the creasing and folding phases. The used standard model accounts for the extremely high anisotropy by means of embedded shell elements, playing the role of reinforcements in the fibre direction. The matrix is assumed to be isotropic and elastoplastic, with properties determined based on the behaviour in the thickness direction. The adopted plasticity model is a modified Drucker–Prager model with a cutoff on the tensile pressure side, available in the used commercial code. The procedure adopted for the identification of the small number of required material parameters is also discussed. Copyright © 2017 John Wiley & Sons, Ltd.

Studies on physico-mechanical properties of W. Murcott mandarin

The post-harvest physico-mechanical properties of fruits is important in adoption and design of varioushandling, packaging, storage and transportation systems. These are important for sizing and grading the fruit beforemarketing as well as demarcate their end use. Geometric, gravimetric and textural properties were determined forthe fresh samples of W. Murcottmandarin. The results show that W. Murcottfruit diameter varies from 81.82-68.97mm, equatorial length varies from 86.51-68.43 mm, and length varies from 61.33-52.08 mm. The specific mean areais 57.591 with the sphericity of 0.90. The L, a, b values were 53.37, 42.9 and 49.68 respectively. The firmness variedfrom 1.351-1.650 kgf. These physical attributes can be taken into consideration while designing the grading andprocessing equipment.

Physical properties of unshelled, shelled and kernel of velvet tamarind (Dialium guineense) fruit from Nigeria

Physical properties of agricultural products are the most important parameters to determine the optimum design of grading, conveying, processing, and packaging systems. This study was conducted to investigate some post-harvest physical properties of unshelled (black), shelled (yellow) and kernel of velvet tamarind (Dialium guineense) fruit. The physical properties studied include axial dimensions, geometric mean diameter (GMD), arithmetic mean diameter, square mean diameter, equivalent mean diameter, surface area, aspect ratio, specific surface area, sphericity, mass, volume, density, porosity, moisture content, angle of repose and coefficient of static friction on various surfaces. The mean length, width, and thickness of velvet tamarind fruit (unshelled) were found to be 17.4, 16, and 8.1 mm respectively; while for the kernel they were 7.7, 7.2, and 3.6 mm, respectively. The sphericity, porosity, bulk density, and angle of repose of tamarind fruit (unshelled) were 0.75, 12.5%, 843.7 kg/m3, and 31.5° respectively. The GMD, surface area, and aspect ratio were 13 mm, 46.3 x 10−5 m2, and 0.93 respectively for the unshelled fruit; but were 9.1 mm, 22.3 × 10−5 m2, and 0.67 respectively for the shelled fruit; and were 5.8 mm, 9.2 × 10−5 m2, and 0.94 respectively for the kernel.

An investigation on the aggregate-shape embedded piezoelectric sensor for civil infrastructure health monitoring

In this paper, a new type of aggregate-shape embedded piezoelectric sensor for health monitoring of civil infrastructures was developed. The sensor was designed by using piezoelectric ceramic chip as the functional phase, and a new composite material as packaging phase. 3D printing technology was also used for packaging system design and sensor fabrication. The frequency independence, linearity, sensitivity, response rate, and service performance of the sensor were tested by frequency scanning and amplitude scanning. Experimental results show that within the vibration frequency range of common civil engineering structures, the new aggregate-shape embedded piezoelectric sensor has good mechanical and workability properties. Test results also show that the new embedded sensors have very good mechanical-electrical coupling performance, which builds a solid foundation for further application in the civil engineering infrastructures.

Creating Semiconductor Value through Advanced Package Technology

Over the past few years, there has been a significant shift from PCs and notebooks to smartphones and tablets as drivers of advanced packaging innovation. In fact, the overall packaging industry is doing quite well today as a result, with solid growth expected to create a market value in excess of $30B USD by 2020. This is largely due to the technology innovation in the semiconductor industry continuing to march forward at an incredible pace, with silicon advancements in new node technologies continuing on one end of the spectrum and innovative packaging solutions coming forward on the other in a complementary fashion. The pace of innovation has quickened as has the investments required to bring such technologies to production. At the packaging level, the investments required to support the advancements in silicon miniaturization and heterogeneous integration have now reached well beyond $500M USD per year. Why has the investment to support technology innovation in the packaging community grown so much? One needs to look no further than the complexity of the most advanced package technologies being used today and coming into production over the next year. Advanced packaging technologies have increased in complexity over the years, transitioning from single to multi-die packaging, enabled by 3-dimensional integration, system-in-package (SiP), wafer-level packaging (WLP), 2.5D/3D technologies and creative approached to embedding die. These new innovative packaging technologies enable more functionality and offer higher levels of integration within the same package footprint, or even more so, in an intensely reduced footprint. In an industry segment that has grown accustomed to a multitude of package options, technology consolidation seems evident, producing “The Big Five” advanced packaging platforms. These include low-cost flip chip, wafer-level chip-scale package (WLCSP), microelectromechanical systems (MEMS), laminate-based advanced system-in-package (SiP) and wafer-based advanced SiP designs. This presentation will address ‘The Big Five’ packaging platforms and how they are adding value to the Semiconductor Industry.

Decomposition analysis of the multidisciplinary coupling in LED System-in-Package design using a DSM and a specification language

LED System-in-Package (SiP) aims to reduce manufacturing and material costs of LED lighting products through integration of components into one single package, based on semiconductor technology. This introduces multidisciplinary coupling in the system behavior which requires reconsideration of the existing LED design decomposition practice. This paper presents our method to do a decomposition analysis of the multidisciplinary coupling structure for an industry scale LED SiP design problem. The innovative aspect of our method is the use of a specification language to specify the input-output (binary) relationships between design variables, behavioral responses, objective and constraint responses. A design structure matrix, representing the mutual linkage, is automatically generated from the specification. The rows and columns of the DSM are subsequently re-ordered using partitioning and sequencing algorithms to provide insight in the coupling structure. The method is illustrated by means of a simplified example. Subsequently, the results for a recently manufactured LED SiP design prototype are presented.

Evaluation of Physicochemical Attributes of Potato (Solanumtuberosum L.) Varieties in Eastern Ethiopia

In Ethiopia, a number of improved potato varieties have been released by different research centre and institution; with much emphasis for adaptability, productivity and late blight resistance, while it has been given less or no emphasis to physicochemical attributes. Therefore, field and laboratory studies were conducted to evaluate the effect of the growing environment on physicochemical attributes of 16 released potato varieties (Moti, Belete, Bubu, Ararsa, Gudenie, Bule, Gabissa, Marachare, Harchassa, Gera, Gorrebella, Guassa, Jalenie, Bedassa, ZemenC all in eastern part of Ethiopia. The experiment was laid out in a Randomized Complete Block Design with three replications. The data were statistically tested and the result indicated the existence of statistically significant difference among genotypes for most of the traits. The maximum tuber geometric mean diameter (57.12mm3), dry matter content (30.66%), starch content (79.267%) and specific gravity (1.0967) were observed for Belete variety. While the highest sphercity (92.00%) and pH value (6.8) were observed for Gera and Bule variety respectively. In the same manner, the highest surface area (17805.7mm2) was observed for Moti variety where as the highest total soluble solid content (7.340Brix) were observed Bubu variety. The result of the study revealedthat the genotype and the growing environment have a great influence on physicochemical attributes of potato tubers. This study suggested that the growing environment has significant influence on potato tuber physicochemical attributes. Therefore, testing genotypes across location becomes important to recommend varieties for design of grading, handling, processing and packaging systems

Wavelet analysis to decompose a vibration simulation signal to improve pre-distribution testing of packaging

In general, vehicle vibration is non-stationary and has a non-Gaussian probability distribution; yet existing testing methods for packaging design employ Gaussian distributions to represent vibration induced by road profiles. This frequently results in over-testing and/or over-design of the packaging to meet a specification and correspondingly leads to wasteful packaging and product waste, which represent $15bn per year in the USA and €3bn per year in the EU. The purpose of the paper is to enable a measured non-stationary acceleration signal to be replaced by a constructed signal that includes as far as possible any non-stationary characteristics from the original signal. The constructed signal consists of a concatenation of decomposed shorter duration signals, each having its own kurtosis level. Wavelet analysis is used for the decomposition process into inner and outlier signal components. The constructed signal has a similar PSD to the original signal, without incurring excessive acceleration levels. This allows an improved and more representative simulated input signal to be generated that can be used on the current generation of shaker tables. The wavelet decomposition method is also demonstrated experimentally through two correlation studies. It is shown that significant improvements over current international standards for packaging testing are achievable; hence the potential for more efficient packaging system design is possible.

Development of a small and flexible sensor-based respirometer for real-time determination of respiration rate, respiratory quotient and low O2 limit of fresh produce

Information on the respiration rate and the low O2 limit (LOL) is important for optimization of packaging and storage systems for fresh fruit and vegetables. In this study, a small and flexible sensor-based respirometer was developed for real-time determination of the respiration rate, respiratory quotient (RQ), and LOL of fresh produce. The respirometer consisted of a wide mouth 1-L glass jar with a screw-type metal lid and an electrochemical and an infra-red sensor mounted directly on the lid of the glass jar to take continuous and non-invasive measurements of the O2 and CO2 contents. Data from the respirometer was compared with data obtained from two fluorescence-based spot sensors (OpTech and PreSens) and a headspace gas analyzer (CheckMate). A test with strawberry showed that similar respiration rates (14.1–16.2mLO2kg−1h−1 and 13.4–16.4mLCO2kg−1h−1 at 10°C) were obtained with all instruments. Further on, a Savitzky–Golay smoothing filter was implemented on the data from the respirometer to estimate the real-time respiration rate. The result showed that the respiration rate could be acquired in 2–3h after filling of the respirometer or even after 1h if the produce was equilibrated to the target storage temperature before the measurements. Detailed information on the respiration rate of wild rocket, strawberry, and carrot showed that the respiration rate decreased with time as the O2 content decreased; however, the RQ remained almost constant throughout storage until the LOL was reached. Information on the RQ and the LOL value is rare in the literature; however, the RQ and the LOL could easily be determined by the use of the respirometer. The RQ was 1.0, 1.0–1.5, and 0.5 for wild rocket, strawberry, and carrot, respectively, during storage under an O2 content above >2.0kPa. As the O2 content dropped to 0.5, 1.0 and 2.0kPa O2, for wild rocket, strawberry, and carrot, respectively, the RQ values increased sharply. The described respirometer made it easy to analyze the impact of a dynamic temperature and O2 content on the respiration rate, the RQ, and the LOL as handling was limited and real-time data could be obtained. With such detailed information, a knowledge-intensive design of packaging and storage systems for fresh horticultural produce is enabled.

اثر ضدمیکروبی فیلم زیست‌فعال زئین حاوی اسانس آویشن علیه Escherichia coli و listeriainnocua طی نگهداری سالاد الویه

Introduction: Antimicrobial packaging is a novel preservation technology that is designed base on releaseing of active agents incorporated with the packaging polymer into the packaged food or the surrounding environment for improveing the quality and food safety. Zein, water insoluble proteinof cornof corn, reported to be thermoplastic material with high tensile strength, excellent hydrophobic film, biodegradability.Zataria multiflora Boiss. (Z) is a thyme-like plant belonging to the Laminaceae family that geographically grows wild in central and southern parts of Iran. Antimicrobial activity of Z essential oil (ZEO) has been successfully tested against foodborne pathogens. Most of the studies on antimicrobial packaging mainly focused on the initial screening of newly developed films for ZEO in laboratory media and quantifying the bacterial reductions obtained during storage for different types of packaged food products. it is really important to know the variation in antibacterial activity of the agents when incorporated into the packaging film from its original activity in order to establish the levels that need to be incorporated for effective bacteria inhibition, hence The aims of the present study were to investigate the antimicrobial potential of ZEO incorporated zein film against pathogenic microorganisms inoculated in olivier saladto provide evidence of their applicability in the design of active food packaging systems. Materials and method: Z was collected in the Shiraz province of Iran and extected by using clevenger type apparatus. Chemical composition ZEO was analyzed by gas chromatography.Antibacterial activity of ZEO was studied in the liquid (direct contact) and solid media (vapour phase) against Listeriainnocua and Escherchia coli . Zein powder was dissolved in a hydro alcoholic solution to obtain 16% (w/w) films forming solutions. The solution was stirred for at 80 °C using a magnetic stirrer hotplate. Glycerol (15%) was added to the solution and stirred again for 8 min at 30°Cm, then ZEO was added to the polymer solutions in 5% and 10% with respect to polymer content and stirred for 8 min. Films were obtained by casting, the film forming solutions were extended over it using an extension bar and introduced in a drying tunnel equipped with a heat of 2500 W during 20 min. Control zein films were prepared without active agent. To evaluate the efficacy of developed zein films was tested against two bacteria. Prior to the experiment, a loop of each strain was transferred to 10 ml of TSB and incubated at 37 ˚C for 18 h to obtain early stationary phase cells and100 µl of microorganism were inoculated into tubes with 10 ml of TSB. The tubes were then incubated at 37 °C for 18 h and 4 °C for 5 days. As control, zein film without active agent was also used in every experiment. Antibacterial activity of zein films containing 10% ZEO were evaluaterd for packaing of olivier salad as a real food model for 6 days, by performing serial dilutions with peptone and subsequent plating in Palcam Listeria Selective Agar and Brilliant Green Agar for ListeriainnocuaandEscherchia colirespectively. Plates were incubated at 37 °C for 48 hours. Results and Discussion: GC–MS analysis showed thatthe major compound of ZEO was carvacrol (45.22%). The antimicrobial effects of ZEO in the vapor phase against Escherchia coliand Listeriainnocuaby using the disk diffusion methodshowed that the25 µg of ZEO produced a visible decrease in microbial density retraction zone (+). No inhibition was observed with the addition of lower amounts of the antimicrobial agents, therefore 25 µg was the minimum inhibitory concentration (MIC).Activezein films containing 5% ZEOweren’t able to inhibit the growth of the two bacteria in the vapour pase.. When the concentrations of ZEO was increased to 10%, only active films with a diameter of 80 mm (50.24 cm2)provided a retraction zone (+) against Listeriainnocuaand E. coli. These values were indicative that incorporation of the agent in the zein films produced weaker vapor phase inhibition than its incorporation in the paper disk.The results of this studied showedthat films with 5% ZEO caused a growth reduction of 1.17 log againstListeriainnocuaand 1.14 log against Escherchia coli, while 10% ZEO produced reductions of 2.16 and 2.64 log against Listeriainnocua, Escherchia colirespectively. There was no significantdifference betweenlog reduction values of ZEO incorporated in zein films against Listeria innocuaand Escherchia coli. And also in the liquid media with increasing the concentration of ZEO in zein films, the log reduction values were increased against both bactreia. Despite of the excellent antibactrerial effect of ZEO in zein films in direct contact, bio active zein films were not effective enough for reducing the contamination of packaged Olivier.