The increasing frequency with which causative agents of systemic and superficial mycoses have been isolated from soil has directed attention to the possibility that it may be an important reservoir of these infectious agents. Much of this work has been directed toward the isolation of dermatophytes which cause diseases of the hair, skin, and nails. These commonly known as ringworm fungi, have a wide geographical distribution and are the causative agents of superficial mycoses such as athlete's foot, dhobie itch, and ringworm. Dermatophytes have the peculiar ability of utilizing keratin, a protein resistant to digestion by the enzymes rennin and pepsin and insoluble in dilute acids and alkalines, as their sole source of carbon and nitrogen. Ajello (1953) reported the successful isolation of the dermatophyte Microsporum gypseum from soil baited with hair and using an isolation medium containing antibiotics. His method extended the work of Vanbreuseghem (1952) who was able to isolate dermatophytes from soil by placing hair filaments of human or animal origin upon the surface of moistened soils. Vanbreuseghem found that other pathogenic fungi such as Candida albicans, Monosporium apiospermum (Allescheria Boydii), and Piedraia Hortia were able to develop on hairs and show their characteristic morphology; however, they did not penetrate the hair filaments. It was possible from the study of hair penetration to make a diagnosis of the dermatophytic nature of the fungus. This hair baiting technique was described as a specific diagnostic method for the identification of dermatophytes. In the present paper the isolation of 4 strains of Microsporum gypseum, 11 strains of Ctenomyces spp., and Monosporium apiospermum, the etiological agent of granular mycetoma, is described. These fungi were isolated from soils using the hair baiting technique, and isolation media containing antibiotics.