The study investigated the amino acid and wound healing potentials of the leaves of Heterotis rotundifolia. Amino acid analysis was performed using HPLC. Total protein level, body weight and histology were studied. Excision wound(2cm) was induced on the dorsal skin of 75 wistar rats (176-308g) divided into five groups of fifteen animals: control, 0.1g Vaseline, 0.1g gentamicin (reference drug), 0.1g extract+0.1g Vaseline (HRE1) and 0.2g extract+0.1g Vaseline (HRE2). At the end of days 1, 7, 14 and 21, rats were sacrificed and blood was collected for wound healing parameters while wound surface was taken for histology. The result revealed a higher total protein level of HRE1 with a significant difference (p<0.05) on day 21 compared to control and days 1-7 compared to gentamicin. Body weight in HRE2 significantly increased from days 1-21 with a significant difference (p<0.05) on days 1-7 compared to control and 1-14 compared to gentamicin. Histological examination revealed faster collagen deposition and angiogenesis on day 14 in HRE1 compared to control and gentamicin. Eighteen amino acids were detected with aspartate, methionine and arginine, (12.94, 9.38 and 8.74mg/100g respectively) having the highest concentrations. Heterotis rotundifolia leaves contains essential amiono acids and has wound healing potentials.