A method is described for the fabrication of non-centrosymmetric multilayer Langmuir-Blodgett (LB) films which are of interest for potential pyroelectric, piezoelectric and non-linear optical device applications. The substrate assembly is attached to a motorized device which is capable of both vertical and horizontal motion. Leaf springs, positioned in a barrier separating two spread monolayers (A and B), prevent the monolayers from mixing whilst allowing easy horizontal transfer of the immersed substrate between trough compartments with the substrate support rod passing between the springs. This mechanism enables the substrate to be cycled through A and B in an arbitrary sequence, and (i) minimizes the risk of unwanted cross-contamination of A and B (quantified using a fluorescent dye), (ii) maintains conventional vertical motion of the substrate through the spread monolayers and (iii) allows the rapid build-up of multilayer films, the rate in general being limited by the slower of the two monolayer deposition speeds. Flow of spread monolayers has been visualized for the new trough during the deposition of multilayer LB films, and the results are correlated with film morphologies observed using an optical birefringence technique; implications for film growth are discussed. Non-centrosymmetric alternating layer structures have been fabricated and, as expected, these films exhibited pyroelectric behaviour.