Dental Plaque Research Articles

Randomized controlled clinical trial on the efficacy of a novel antimicrobial chewing gum in reducing plaque and gingivitis in adolescent orthodontic patients

ObjectivesChewing gums containing antiseptics or other antimicrobial substances may be effective in reducing plaque and gingivitis. Therefore, the aim of this randomized placebo-controlled clinical trial was to investigate the efficacy of a novel antimicrobial chewing gum containing essential oils (cinnamon, lemon, peppermint) and extracts on reduction of dental plaque and gingivitis as well as on oral health-related quality of life (OHRQoL) in adolescent orthodontic patients.Materials52 patients (11-22 years of age) were randomly assigned to use a test chewing gum (COVIDGUM, Clevergum) or a commercially available control chewing gum over a period of 10 days. Approximal plaque index (API), papillary bleeding index (PBI) and an OHRQoL questionnaire for children (COHIP-G19) were assessed at baseline (BL), after 10 days (10d) and 30 days (30d). In addition, oral health and oral hygiene related questions of the COHIP-G19 questionnaire were evaluated separately in subscales at each timepoint. Data were analyzed using non-parametrical statistical procedures (α = 0.05).ResultsAPI and PBI decreased significantly over time from BL to 10d and from BL to 30d in both groups, without significant differences between the groups. In both groups, the COHIP-G19 score, oral health subscale and oral hygiene subscale decreased significantly over time. Regarding the oral hygiene subscale, the test group showed significantly better scores at 30d (p = 0.011).ConclusionBoth chewing gums performed similarly effective in terms of reducing plaque accumulation and gingival inflammation and improving OHRQoL.Clinical relevanceChewing gums without antimicrobial ingredients may be sufficient to decrease plaque accumulation and gingival inflammation.

Comparison of oral health status, oral hygiene management behaviours and satisfaction of patients with fixed orthodontic appliance and clear aligner: A quasi-experimental design.

This study aimed to determine the overall oral health statuses of patients with fixed orthodontic appliance and clear aligner, as well as their oral hygiene management behaviours and satisfaction. We selected 40 participants (20 each with fixed orthodontic devices and clear aligners) who visited a dental clinic. We conducted a survey to determine the oral hygiene management behaviours of the subjects and their satisfaction with their orthodontic treatment. Three measurements were also conducted at 4-week intervals to determine the oral health statuses of the subjects. The Löe and Silness gingival index of patients with fixed orthodontic appliances indicated moderate gingivitis (1.1 ± 0.3), and those with clear aligners had mild gingivitis (0.6 ± 0.4) (p < 0.001). The modified O'Leary index also indicated that the degree of dental plaque deposition in the oral cavity was lower in patients treated with a clear aligner (43.0 ± 18.2 points) than in those treated with a fixed orthodontic (28.1 ± 10.9 points) (p = 0.004). Regarding oral hygiene management, the fixed orthodontic group received scaling more frequently (p = 0.006), received more oral health education (p < 0.001) and had a longer brushing time (p = 0.008) than the clear aligner group. No significant difference was observed in satisfaction between the fixed orthodontic appliance and the clear aligner. Regarding the oral health status by orthodontic appliance type, the clear aligner group had the advantages of reduction in dental plaque attachment and gingival health. The fixed orthodontic group exhibited better oral hygiene management behaviours. Satisfaction with orthodontic treatment was found to be similar for the two device types.

Benzydamine hydrochloride: an overview on a well-established drug with news in mechanisms of action

Pain and inflammation are the consequences of sore throat, dental and oral procedures, infections, ulcers and head and neck chemotherapy/radiotherapy, and their management is of fundamental importance to avoid distress in patients. Benzydamine hydrochloride (HCl) is a topical indolic nonsteroidal anti-inflammatory drug, endowed with analgesic and anesthetic activity, and with antimicrobial (including both gram-positive and gram-negative bacteria) and antifungal properties (targeting Candida albicans and non-albicans strains), used in odontostomatology, otorhinolaryngology, and gynecology for its properties. This molecule has a lipophilic nature, showing high affinity with cell membranes and exhibiting membrane stabilization properties, resulting in local anesthesia, an effect related also to the interaction of the drug with cationic channels. In addition, benzydamine HCl is able to inhibit the production of pro-inflammatory cytokines, with consequent analgesia. Moreover, benzydamine HCl is able to inhibit leukocyte-endothelial interactions and platelet aggregation. Unlike other non-steroidal anti-inflammatory drugs, benzydamine HCl does not inhibit cyclooxygenase or lipoxygenase. Here we review the most updated clinical data available on benzydamine HCl local application as spray, mouthwash or gargling and evidence of its effectiveness in inflammatory and/or septic conditions in the otorhinolaryngology and odontostomatology settings, with particular reference to sore throat, oral inflammation, dental plaque, tonsillitis/tonsillectomy and chemo- or radiotherapy-induced oral mucositis. Novel formulations for oral administration of benzydamine HCl are also reviewed, including in situ gelling formulations to be sprayed onto the damaged oral mucosa. Finally, novel data on the potential role of benzydamine HCl in nociceptor excitability are introduced.

THE EFFECTIVENESS OF METRONIDAZOLE GELS IN THE MANAGEMENT OF PERIODONTAL DISEASE

Introduction : Metronidazole is one of the antimicrobial and antimicrobial that has been widely used as antibacterial and antiprotozoal agent for treatment. Metronidazole is antimicrobial have been used as an adjuvant to mechanical plaque removal. Periodontal disease is a chronic inflammatory disease that develops from dental plaque that affects periodontal supporting tissues. Many chemical agents that have been evaluated to determine their effect on plaque, gingivitis and periodontitis, one of which is metronidazole, has been successfully introduced and studied extensively. This literature review explains effectiveness of metronidazole gels in the management of periodontal disease. Review : Metronidazole (MTZ) is frequently used in the treatment for periodontal diseases since it can be against several Gram-negative anaerobic rods, the pathogenesis of periodontitis. It can be administered via oral and topical routes. However, the systemic adverse effects may in the oral cavity if used peroral. Hence, topical administration is preferable. The bacterial reservoirs not eliminated by conventional periodontal therapy can be further suppressed with the use of chemotherapeutic agents. Localized antimicrobial therapy, in particular, has evoked growing interest because of the site‑specific nature of periodontal infections, the higher concentration of anti‑microbial agent subgingivally and reduced side‑effects of systemic antibiotic use. Among the various locally delivered chemotherapeutic agents metronidazole, a has bactericidal action against anaerobes, such as Prevotela intermedia, Porphyromonas gingivalis, Tannerella forsythia, Fusobacterium species and spirochetes like Treponema denticola, Treponema vincentii, which are generally believed to be the main pathogens associated with periodontitis. Conclusion : Treatment of periodontal pockets with metronidazole gel in addition to scaling and root planning, provides a significant increase in reducing pocket depth and microbial count and effective in the treatment of periodontal disease.

MANAGEMENT OF BACTERIAL PLAQUE-INDUCED GINGIVAL ENLARGEMENT: CASE REPORT

Introduction: Gingival enlargement is a condition that we often encounter in the oral cavity. This condition can be caused by plaque accumulation, systemic disorders, and side effects of some drugs as well as genetic factors. Gingival enlargement due to the accumulation of bacterial plaque generally shows signs and symptoms of inflammation which can be a major risk factor for periodontitis. This case report aims to describe two cases of plaque-induced gingival enlargement, one of which was accompanied by the presence of granulomatous epulis. Case: The first case was a 23-year-old woman complaining that her gums often bleed when brushing her teeth since 1 year ago. Intra oral examination can see swelling of the gingiva in the anterior area of RA and RB. The second case is a 40-year-old woman with complaints of swelling of the lower right back gum since 2 years ago. An enlargement of the gingiva in the anterior area is seen accompanied by granulomatous epulis with fibrous consistency in the posterior area of RB. Treatment: In the first case, non-surgical treatment was carried out and continued with regular visits every 3 months. In the second case, non-surgical and surgical procedures were performed. Treatment begins with tooth root extraction and scaling and root planning. Furthermore, gingivectomy surgery was performed to excise the enlarged gingiva. Discussions: The treatment results look good, where it can be seen that the etiological factor can be ruled out. After the surgical treatment, the healing went well, marked by no inflammation around it. Conclusion: Gingival enlargement can affect aesthetics and interfere with the patient's masticatory function. Identification of the etiological factors is necessary for an appropriate treatment plan.

Genomic and Resistome Analyses of Elizabethkingia anophelis Strain B2D isolated from Dental Plaque of Patient

In this study, strain B2D isolated from a dental plaque sample of a human patient was studied for its general characteristics, taxonomic identification, genome features, and resistome profile. The bacterium exhibited antibiotic resistance to all beta-lactam antibiotics, nitrofuran, and sulfonamides, with high minimum inhibitory concentrations. It was only sensitive to the fluoroquinolone ciprofloxacin and intermediately susceptible to aminoglycoside tobramycin. A preliminary identification through 16S rRNA gene sequences revealed that it shared the highest sequence identity with Elizabethkingia anophelis subsp. endophytica JM-87T (100%) and Elizabethkingia anophelis subsp. anophelis R26T (99.31%). The draft genome of strain B2D was approximately 3.9 Mbp with 50 contigs and 35.5% GC content. A 16S rRNA gene and core genes-based phylogenetic analyses revealed a close phylogenetic relationship between strain B2D and the other Elizabethkingia type strains. An above species level threshold average nucleotide identity value confirmed its taxonomic identity as Elizabethkingia anophelis. Furthermore, we conducted a resistome analysis of strain B2D and Elizabethkingia type strains, revealing the presence of widespread antibiotic resistance genes, including beta-lactamases and genes associated with cationic antiseptic resistance and glycopeptide resistance. Overall, the multidrug resistant profile of strain B2D as elucidated and confirmed through whole genome analysis indicated its potential as a reservoir of beta-lactamase genes. Moreover, its presence within dental plaque in the human oral cavity prompts speculation regarding its role as an opportunistic pathogen capable of causing infections, particularly in immunocompromised individuals.

Transcriptomic features of programmed and inflammatory cell death in gingival tissues.

This study used an experimental periodontitis model to detail the gingival transcriptome related to cell death processes of pyroptosis, necroptosis, ferroptosis, and cuproptosis. Healthy Macaca mulatta primates stratified by age, ≤3 years (young), 7-12 years (adolescent), 12-15 years (adult), and 17-23 years (aged), provided gingival tissue biopsies for microarray analysis focused on 257 genes representative of the four cell death processes and bacterial plaque samples for 16S rRNA gene analysis. Age differences in the profiles of gene expression in healthy tissues were noted for cuproptosis, ferroptosis, necroptosis, and pyroptosis. Major differences were then observed with disease initiation, progression, and resolution also related to the age of the animals. Distinct bacterial families/consortia of species were significantly related to the gene expression differences for the cell death pathways. These results emphasized age-associated differences in the gingival tissue molecular response to changes in the quality and quantity of bacteria accumulating with the disease process reflected in regulated cell death pathways that are both physiological and pathophysiological.

Substrate geometry affects population dynamics in a bacterial biofilm

Biofilms inhabit a range of environments, such as dental plaques or soil micropores, often characterized by noneven surfaces. However, the impact of surface irregularities on the population dynamics of biofilms remains elusive, as most experiments are conducted on flat surfaces. Here, we show that the shape of the surface on which a biofilm grows influences genetic drift and selection within the biofilm. We culture Escherichia coli biofilms in microwells with a corrugated bottom surface and observe the emergence of clonal sectors whose size corresponds to that of the corrugations, despite no physical barrier separating different areas of the biofilm. The sectors are remarkably stable and do not invade each other; we attribute this stability to the characteristics of the velocity field within the biofilm, which hinders mixing and clonal expansion. A microscopically detailed computer model fully reproduces these findings and highlights the role of mechanical interactions such as adhesion and friction in microbial evolution. The model also predicts clonal expansion to be limited even for clones with a significant growth advantage-a finding which we confirm experimentally using a mixture of antibiotic-sensitive and antibiotic-resistant mutants in the presence of sublethal concentrations of the antibiotic rifampicin. The strong suppression of selection contrasts sharply with the behavior seen in range expansion experiments in bacterial colonies grown on agar. Our results show that biofilm population dynamics can be affected by patterning the surface and demonstrate how a better understanding of the physics of bacterial growth can be used to control microbial evolution.

The application of phenylboronic acid pinacol ester functionalized ROS-responsive multifunctional nanoparticles in the treatment of Periodontitis

Periodontitis is an inflammatory disease induced by the complex interactions between the host immune system and the microbiota of dental plaque. Oxidative stress and the inflammatory microenvironment resulting from periodontitis are among the primary factors contributing to the progression of the disease. Additionally, the presence of dental plaque microbiota plays a significant role in affecting the condition. Consequently, treatment strategies for periodontitis should be multi-faceted. In this study, a reactive oxygen species (ROS)-responsive drug delivery system was developed by structurally modifying hyaluronic acid (HA) with phenylboronic acid pinacol ester (PBAP). Curcumin (CUR) was encapsulated in this drug delivery system to form curcumin-loaded nanoparticles (HA@CUR NPs). The release results indicate that CUR can be rapidly released in a ROS environment to reach the concentration required for treatment. In terms of uptake, HA can effectively enhance cellular uptake of NPs because it specifically recognizes CD44 expressed by normal cells. Moreover, HA@CUR NPs not only retained the antimicrobial efficacy of CUR, but also exhibited more pronounced anti-inflammatory and anti-oxidative stress functions both in vivo and in vitro. This provides a good potential drug delivery system for the treatment of periodontitis, and could offer valuable insights for dental therapeutics targeting periodontal diseases.

Applications of Nanotechnology in the Immunogenic Detection of Allele Risk Gene MBL2 in Teeth Plaque Patients

Applications of Nanotechnology in the Immunogenic Detection of Allele Risk Gene MBL2 in Teeth Plaque Patients

Impact of clear aligners on plaque and periodontal health

In recent years, clear aligners have become a well-liked substitute for traditional braces in orthodontic treatment. Examining the effects of clear aligners on periodontal health and plaque accumulation is the goal of this review. To examine pertinent studies that have been published up to this point, a comprehensive evaluation of the literature was carried out. The review includes research on the microbial alterations in tooth plaque composition that occur during clear aligner therapy and the subsequent impact on periodontal tissues. Clear aligners and traditional braces are also compared with respect to how they affect gingival health, periodontal disease prevalence, and oral hygiene practices.In terms of plaque control, the results imply that transparent aligners offer special benefits and challenges. An important factor influencing the microbiological environment in the oral cavity is patient compliance, the type of aligner used, and the subtleties of its design. Moreover, the effect of orthodontic therapies on periodontal health is explored, providing insight into possible associations between the use of clear aligners and periodontal results. By underlining the necessity of individualized oral hygiene management strategies during clear aligner therapy, this review offers orthodontic practitioners’ insightful information. It highlights the need of maintaining periodontal health throughout orthodontic treatment and stresses the necessity of a multidisciplinary approach to optimize patient outcomes. In summary, this comprehensive review provides an overview of the most recent findings regarding the impact of clear aligners on plaque and periodontal health. By teaching clinicians, researchers, and patients about the subtleties of oral hygiene care during orthodontic treatment, the findings are meant to direct the development of effective strategies for maintaining good periodontal health in patients getting clear aligner therapy.

Detailed structural characterization of five water-insoluble α-glucans produced by glucansucrases from Streptococcus spp.

Water-insoluble α-glucans synthesized from sucrose by glucansucrases from Streptococcus spp. are essential in dental plaque and caries formation. Because limited information is available on the fine structure of these biopolymers, we analyzed the structures of unmodified glucans produced by five recombinant Streptococcus (S.) mutans DSM 20523 and S. salivarius DSM 20560 glucansucrases in detail. A combination of methylation analysis, endo-dextranase and endo-mutanase hydrolyses, and HPSEC-RI was used. Furthermore, crystal-like regions were analyzed by using XRD and 13C MAS NMR spectroscopy. Our results showed that the glucan structures were highly diverse: Two glucans with 1,3- and 1,6-linkages were characterized in detail besides an almost exclusively 1,3-linked and a linear 1,6-linked glucan. Furthermore, one glucan contained 1,3-, 1,4-, and 1,6-linkages and thus had an unusual, not yet described structure. It was demonstrated that the glucans had a varying structural architecture by using partial enzymatic hydrolyses. Furthermore, crystal-like regions formed by 1,3-glucopyranose units were observed for the two 1,3- and 1,6-linked glucans and the linear 1,3-linked glucan. 1,6-linked regions were mobile and not involved in the crystal-like areas. Altogether, our results broaden the knowledge of the structure of water-insoluble α-glucans from Streptococcus spp.

The effect of individuals' oral hygiene habits and knowledge levels on peri-implant health and disease: a questionnaire-based observational study

BackgroundPeri-implant disease and health are associated with microbial dental plaque. Therefore, oral hygiene plays a role in preventing and treating these diseases. This study aimed to determine the relationships among knowledge of peri-implant status, oral hygiene habits, and peri-implant disease and health.MethodsA total of 144 implants in nonsmokers with controlled systemic disease were included in the study. Peri-implant disease and the conditions of the implants were determined with periodontal indices and radiographs based on the 2017 World Workshop on the Classification of Periodontal and Peri-implant Diseases and Conditions and The EFP S3 level clinical practice guideline. Individuals were asked 66 questions regarding demographic information, oral hygiene habits and history, and knowledge of peri-implant diseases. One-way ANOVA was used to compare the three peri-implant disease and condition categories.ResultsThere was a significant difference between groups regarding toothpaste type (p < 0.05). Gum protection toothpaste was greater in the peri-implant health group. Patients' use of interdental products was very low; often, no products were used for implant prosthesis. There was no significant difference among the groups regarding oral hygiene product use or oral hygiene habits (p > 0.05). There was a significant difference between groups regarding frequency of visit (p < 0.05). The frequency of visits to the dentist for pain was greater for individuals with peri-implantitis. There is a significant difference between the groups' answers for the causative and initiating factors of peri-implant disease (p < 0.05). The peri-implant health group answered that microbial dental plaque is the most crucial initiating factor of peri-implant diseases, and bleeding on probing is the most critical determinant of peri-implant diseases at a higher rate than the other groups.ConclusionsPatients' oral hygiene habits and knowledge levels are almost similar according to peri-implant status. Knowledge does not reflect a patient's oral hygiene behavior. Clinicians should ensure that individuals' oral hygiene practices align with their increased awareness regarding peri-implant illnesses.

Antibacterial Herbal Mouthwash Formulation and Evaluation Against Oral Disorders

The goal of the present study is to create and assess a herbal mouthwash and determine how well it works to lower oral cavity bacterial counts. Following their collection, the plant materials' water-soluble components were removed. We also assessed the mouthwash's physicochemical characteristics and antibacterial efficacy. The mouthwash that is now available on the market has strong antimicrobial qualities. The stability investigation's findings attest to the preparation's efficacy. These days, mouthwash is marketed as a liquid and usually includes antiseptic and antibacterial ingredients. These solutions have the potential to serve several purposes, including inhibiting the growth of germs in the oral cavity and acting as an analgesic, anti-inflammatory, or anti-fungal agent. The oral cavity is home to a variety of microbes. While certain types of oral bacteria are harmless, others can lead to mouth illnesses, plaque buildup in the mouth, and bad breath. Therefore, keeping good oral hygiene is essential for both physical and mental well-being. The effectiveness of herbs is thought to be relatively high when compared to chemical products. Medicinal plants are crucial in treating a variety of illnesses due to their potent antibacterial and antifungal effects against human infections. Herbal remedies aid in tooth cleaning, reduce dental plaque, inhibit the growth of bacteria, and freshen breath. Herbal mouthwashes can be used in addition to your normal dental hygiene routine, which includes brushing and flossing. They are helpful in supportive periodontal treatment because of their potent antiinflammatory and anti-plaque characteristics. Many herbal remedies and their extracts, including those made from peppermint, cloves, neem, and turmeric, have demonstrated notable benefits over synthetic alternatives. Due to their long-lasting anti-bacterial, antimicrobial, and anti-fungal actions on human pathogens, medicinal herbs are essential for the treatment of illness. Using natural mouthwash in place of conventional ones may have several advantages. If a formulation like this could be invented that folks could safely and simply prepare at home using natural ingredients. As a result, the general oral health of the community could get better. Because they function on oral infections, quickly relieve pain, have few or no side effects, and target oral pathogens, herbal mouthwashes are quite popular. Among the most common infectious diseases that many individuals encounter at different times in their life are dental caries and periodontal disease. The purpose of this article is to give a general review of natural substances that can be effective mouthwashes. The objective of this study was to use polyherbal components to make mouthwash that has antibacterial properties.

Exploring the influence of microbiota on gestational diabetes and its potential as a biomarker

Gestational diabetes mellitus (GDM) represents a significant health concern during pregnancy, impacting both maternal and fetal well-being. While conventional diagnostic protocols typically rely on blood glucose levels in the latter stages of pregnancy, there is a pressing need for early detection methods to mitigate potential risks. A plethora of glucose-based or non-glucose-based biomarkers have been investigated for their potential to predict GDM in early pregnancy. Though specific biomarkers showed promise in predicting GDM, their clinical usage has been constrained by the lack of validation and limitation in translating them into routine clinical use. This review aims to highlight and discuss the potential and practical utility of existing biomarkers and emergent biomarkers, such as microbiomes, in diagnosing GDM. A comprehensive analysis of recent studies reveals significant alterations in the composition and diversity of microbiota among women with GDM, suggesting their potential utility as predictive markers for this condition. For instance, distinct microbial profiles characterized by an increased abundance of Eisenbergiella, Tyzzerella 4, and Lachnospiraceae NK4A136, alongside decreased levels of Parabacteroides, Parasutterella, and Ruminococcaceae UCG 002, correlated with fasting blood glucose levels, hinting at their relevance in early GDM detection. Furthermore, proposed microbiota-targeted panels demonstrated promising predictive accuracy. Beyond gut microbiota, recent investigations have also explored the potential of oral microbiota as predictive biomarkers for GDM. Studies have highlighted the discriminatory capacity of specific oral microbes, such as Streptococcus in saliva and Leptotrichia in dental plaque, in distinguishing GDM from healthy pregnancies. Moreover, the examination of gut microbiota-derived metabolites has shown promising results in serum-based GDM prediction. These findings collectively underscore the potential of microbiota and its metabolites as valuable biomarkers for the early detection of GDM. However, further research is warranted to elucidate the mechanistic links between microbial dysbiosis and GDM pathogenesis, ultimately facilitating the development of targeted therapeutic interventions and personalized management strategies.

Anticaries and Antigingivitis Properties of Cannabinoid-Containing Oral Health Products: A Review.

To evaluate the anticaries and antigingivitis properties of cannabinoid-containing oral health products. A systematic research strategy was employed. Specific search terms were used, including "Cannabinoids AND dental caries," "Cannabinoids AND oral health," "Cannabinoids AND dental plaque," "Cannabinoids AND gingivitis AND periodontitis," "Cannabinoids AND S. mutans," "Cannabidiol AND oral health," and "Cannabidiol AND oral biofilm." The search was conducted in PubMed, Cochrane, and EBSCO Host databases. The search yielded a total of 73 articles, out of which 15 articles (20.5%) were relevant to the scope of this systematic review. Among the relevant articles, only eight (10.9%) directly addressed the research question. The findings from these articles suggest that cannabinoids have the potential to reduce the metabolism of cariogenic bacteria, specifically Streptococcus mutans, and decrease the number of bacterial colonies in dental plaque. In vitro studies also demonstrated a significant inhibitory effect of cannabinoids on oral biofilms and create a considerable inhibitory zone of growth when investigated on oral biofilms in vitro. Furthermore, CBD exhibited antibacterial properties against Porphyromonas gingivalis, a primary pathogen associated with periodontal disease. The current review shows insufficient data to conclude on the anticaries and antigingivitis effects of cannabinoids. Despite extensive research on their systemic therapeutic benefits, their oral health impact remains underexplored, lacking clinical trials and primary research.

UiO-66/AgNPs Coating for Dental Implants in Preventing Bacterial Infections

Titanium (Ti)–based biomaterials lack inherent antimicrobial activities, and the dental plaque formed on the implant surface is one of the main risk factors for implant infections. Construction of an antibacterial surface can effectively prevent implant infections and enhance implant success. Silver nanoparticles (AgNPs) exhibit broad antibacterial activity and a low tendency to induce drug resistance, but AgNPs easily self-aggregate in the aqueous environment, which significantly impairs their antibacterial activity. In this study, UiO-66/AgNP (U/A) nanocomposite was prepared, where zirconium metal–organic frameworks (UiO-66) were employed as the confinement matrix to control the particle size and prevent aggregation of AgNPs. The bactericidal activity of U/A against methicillin-resistant Staphylococcus aureus and Escherichia coli increased nearly 75.51 and 484.50 times compared with individually synthesized Ag. The antibacterial mechanism can be attributed to the enhanced membrane rupture caused by the ultrafine AgNPs on UiO-66, leading to protein leakage and generation of intracellular reactive oxygen species. Then, U/A was loaded onto Ti substrates (Ti-U/A) by using self-assembly deposition methods to construct an antibacterial surface coating. Ti-U/A exhibited excellent antibacterial activities and desired biocompatibility both in vitro and in vivo. The U/A nanocomposite coating technique is thus expected to be used as a promising surface modification strategy for Ti-based dental implants for preventing dental implant infections.

Low level of the dental health as an indicator of malignant of the oral mucosa

Introduction. The manifestation of oncological alertness during dispensary observation by a dentist is very important. The most accessible options for preventing malignancy of the oral mucosa are sanitation, regular professional oral hygiene, as well as educational work among doctors and the population. However, despite the visual accessibility of tissues and organs of the oral cavity, there is an annual increase in malignant neoplasms in this area. In this case, most patients are diagnosed with a tumor process of the last stage. malignant neoplasms of the head and neck organs require special attention due to high risk of disability even at an early stage. Aim. To evaluate a correlation between malignancy of the oral mucosa and the burdened dental status of patients. Materials and methods. A controlled study was conducted which included 142 patients with cancer of the oral mucosa without sequential or parallel inclusion of patients in groups. Dental status was assessed at help-seeking stage or during clinical examination, as planned, by evaluation of main dental indices. The morphological diagnosis was established using histological verification. Results. The majority of patients with oral tumors had hard dental plaque (81.7 % of cases). The condition of the soft tissues, in addition to presence of the mucous membrane lesion, was characterized by bleeding (69 % of cases). moderate periodontal pathology was detected (19 % of cases). patients with the cavity tumors had an acute traumatic edge of hard tissues (70 % of cases), extensive caries (26 % of cases) and the presence of removable dentures (82 % of cases). Conclusion. The values of parameters evaluated in patients in the study with squamous cell carcinoma of the oral mucosa show its relation to aggravation of general somatic status. All patients in the study required dental treatment and training in oral hygiene care.

Comparative analysis of microbial composition and functional characteristics in dental plaque and saliva of oral cancer patients

BackgroundThe oral cavity is home to various ecological niches, each with its own unique microbial composition. Understanding the microbial communities and gene composition in different ecological niches within the oral cavity of oral cancer (OC) patients is crucial for determining how these microbial populations contribute to disease progression.MethodsIn this study, saliva and dental plaque samples were collected from patients with OC. Metagenomic sequencing was employed to analyze the microbial community classification and functional composition of the different sample groups.ResultsThe results of the study revealed significant differences in both the function and classification of microbial communities between saliva and dental plaque samples. The diversity of microbial species in saliva was found to be higher compared to that in plaque samples. Notably, Actinobacteria were enriched in the dental plaque of OC patients. Furthermore, the study identified several inter-group differential marker species, including Prevotella intermedia, Haemophilus parahaemolyticus, Actinomyces radius, Corynebacterium matruchitii, and Veillonella atypica. Additionally, 1,353 differential genes were annotated into 23 functional pathways. Interestingly, a significant correlation was observed between differentially labeled species and Herpes simplex virus 1 (HSV-1) infection, which may be related to the occurrence and development of cancer.ConclusionsSignificant differences in the microbial and genetic composition of saliva and dental plaque samples were observed in OC patients. Furthermore, pathogenic bacteria associated with oral diseases were predominantly enriched in saliva. The identification of inter-group differential biomarkers and pathways provide insights into the relationship between oral microbiota and the occurrence and development of OC.

HELICOBACTER PYLORI – BIOLOGICAL FEATURES AND METHODS OF LABORATORY DIAGNOSIS

The spring period - in that time the relevance of diagnostics related to Helicobacter pylori infection forces us to pay more attention to the cohort of patients with gastrointestinal tract pathology. Most often, these are patients with exacerbation of chronic gastritis (CH) and peptic ulcer disease (UD) of the stomach and duodenum, the typical course of which involves the seasonality of exacerbations: spring and autumn. Among other criteria of a "typical" course, infection with H. pylori, which is the cause of these diseases and without its destruction in the body, it is impossible to achieve clinical remission of H. pylori-associated diseases and prevent their recurrence. Before prescribing therapy, the causative agent must be identified, and after treatment, its eradication must be confirmed. The severity of chronic diseases of helicobacterial etiology depends on the degree of pathogenicity of the strains, the presence of certain cytotoxic genes. The review analyzes modern information on the biological properties of the causative agent of helicobacteriosis and methods of its diagnosis. They can be divided into invasive (requires taking a biopsy during endoscopic examination) and non-invasive. Bacteriological and morphological research methods are distinguished among the invasive ones. The histological method is recognized as the "gold standard" for the diagnosis of helicobacteriosis. The essence of the method consists in the preparation of preparations of the gastric mucosa and their Giemsa staining in order to detect bacterial cells in the preparation. The method allows you to determine the characteristics of the causative agent and assess the condition of the gastric mucosa. The bacteriological method is considered indispensable for checking strains for resistance to certain antibacterial drugs, which allows predicting the results of treatment. Currently, non-invasive diagnostic methods have become the most widespread. Along with the respiratory urease test, serological methods are used (immunoenzyme analysis, immunoblotting), as well as the immunochromatographic method. The molecular diagnostic method, namely PCR, is used to study the genotypic and phenotypic characteristics of H. pylori strains in gastric biopsy samples, saliva, stools, gastric juice, and dental plaque. PCR provides excellent sensitivity and specificity of over 95% compared to other tests.