Denitrification In Zone Research Articles

Landform, soil, and plant relationships to nitrate accumulation, Central Nevada

Nitrate (NO 3 − 1 ) accumulates in Haplocambids and Torrifluvents in inset fan and fan skirt positions in central Nevada. The soils store as much as 17,600 kg of NO 3 − 1 N ha − 1 within the upper 208 cm. This paper provides an explanation. These Holocene soils receive NO 3 − 1 N from mineralization of organic matter and other NO 3 − 1 N sources including snowmelt. The NO 3 − 1 is delivered to soils in the first part of snowmelt in run-off from the higher surfaces. The last part of the melt and the run-off, when sufficient, serve to move the NO 3 − 1 out of the root zone. Winter fat (Krascheninnikovia lanata), the most valuable winter grazing plant in the Great Basin, is the common plant on NO 3 − 1 N rich soils. The soils are loamy or sandy and lack horizons restricting water penetration or biological denitrification zones. Hence, some NO 3 − 1 is free to leach deeply past plant roots. Playas, wet floodplains, deeply gullied inset fans and well-developed soils accumulate little NO 3 − 1 except where the latter soils are capped by desert pavements and rarely, if ever become saturated with water. Soils with argillic or petrocalcic horizons or duripans on summits of alluvial fan remnants loose NO 3 − 1 through denitrification, or incorporate it in plants, commonly accumulating less than 50 kg of NO 3 − 1 N ha − 1 . These soils however do accumulate salt as shown by their shadscale saltbush Atriplex confertifolia, bud sagebrush Picrothamnus desertorum, and four-wing saltbush Atriplex conescens shrub cover.

Simulating the global distribution of nitrogen isotopes in the ocean

We present a new nitrogen isotope model incorporated into the three‐dimensional ocean component of a global Earth system climate model designed for millennial timescale simulations. The model includes prognostic tracers for the two stable nitrogen isotopes, 14N and 15N, in the nitrate (NO3−), phytoplankton, zooplankton, and detritus variables of the marine ecosystem model. The isotope effects of algal NO3− uptake, nitrogen fixation, water column denitrification, and zooplankton excretion are considered as well as the removal of NO3− by sedimentary denitrification. A global database of δ15NO3− observations is compiled from previous studies and compared to the model results on a regional basis where sufficient observations exist. The model is able to qualitatively and quantitatively reproduce many of the observed patterns such as high subsurface values in water column denitrification zones and the meridional and vertical gradients in the Southern Ocean. The observed pronounced subsurface minimum in the Atlantic is underestimated by the model presumably owing to too little simulated nitrogen fixation there. Sensitivity experiments reveal that algal NO3− uptake, nitrogen fixation, and water column denitrification have the strongest effects on the simulated distribution of nitrogen isotopes, whereas the effect from zooplankton excretion is weaker. Both water column and sedimentary denitrification also have important indirect effects on the nitrogen isotope distribution by reducing the fixed nitrogen inventory, which creates an ecological niche for nitrogen fixers and, thus, stimulates additional N2 fixation in the model. Important model deficiencies are identified, and strategies for future improvement and possibilities for model application are outlined.

Localized zones of denitrification in a floodplain aquifer in southern Wisconsin, USA

A floodplain aquifer within an agricultural watershed near Madison, Wisconsin (USA), was studied to determine whether denitrification was occurring below the surface organic layer. Groundwater levels and concentrations of O2, Cl−, NO3−, SO42−, dissolved organic carbon (DOC), and major cations were monitored over a 1-year period along a 230-m transect between an agricultural field and a stream discharge point. Seventeen groundwater samples were analyzed for δ15NNO3 and δ18ONO3 composition. Samples in which NO3− was too low for stable isotope analysis were analyzed for excess dissolved N2. Groundwater NO3− concentrations declined between the agricultural field and the discharge point. Chloride and δ15NNO3/δ18ONO3 data indicated that the drop in NO3− was caused primarily by dilution of shallow NO3−-rich water with deeper, NO3−-depleted groundwater. Two localized zones of denitrification were identified in the upland-wetland transition by their δ15NNO3 and δ18ONO3 signatures, and two in the stream hyporheic zone by the presence of excess dissolved N2. The combined stratigraphic, hydrologic, and geochemical data in these locations correspond to groundwater mixing zones where NO3− is delivered to subsurface layers that support denitrification fueled by dissolved (e.g. DOC or dissolved Fe(II)) and/or solid-phase (e.g. particulate organic carbon, solid-associated Fe(II), or pyrite) electron donors.

Kinetics of N<sub>2</sub>O production and reduction in a nitrate-contaminated aquifer inferred from laboratory incubation experiments

Abstract. Knowledge of the kinetics of N2O production and reduction in groundwater is essential for the assessment of potential indirect emissions of the greenhouse gas. In the present study, we investigated this kinetics using a laboratory approach. The results were compared to field measurements in order to examine their transferability to the in situ conditions. The study site was the unconfined, predominantly sandy Fuhrberger Feld aquifer in northern Germany. A special characteristic of the aquifer is the occurrence of the vertically separated process zones of heterotrophic denitrification in the near-surface groundwater and of autotrophic denitrification in depths beyond 2–3 m below the groundwater table, respectively. The kinetics of N2O production and reduction in both process zones was studied during long-term anaerobic laboratory incubations of aquifer slurries using the 15N tracer technique. We measured N2O, N2, NO3-, NO2-, and SO42- concentrations as well as parameters of the aquifer material that were related to the relevant electron donors, i.e. organic carbon and pyrite. The laboratory incubations showed a low denitrification activity of heterotrophic denitrification with initial rates between 0.2 and 13 μg N kg−1 d−1. The process was carbon limited due to the poor availability of its electron donor. In the autotrophic denitrification zone, initial denitrification rates were considerably higher, ranging between 30 and 148 μg N kg−1 d−1, and NO3- as well as N2O were completely removed within 60 to 198 days. N2O accumulated during heterotrophic and autotrophic denitrification, but maximum concentrations were substantially higher during the autotrophic process. The results revealed a satisfactory transferability of the laboratory incubations to the field scale for autotrophic denitrification, whereas the heterotrophic process less reflected the field conditions due to considerably lower N2O accumulation during laboratory incubation. Finally, we applied a conventional model using first-order-kinetics to determine the reaction rate constants k1 for N2O production and k2 for N2O reduction, respectively. The goodness of fit to the experimental data was partly limited, indicating that a more sophisticated approach is essential to describe the investigated reaction kinetics satisfactorily.

Performance of Nitrogen-Removing Bioretention Systems for Control of Agricultural Runoff

This research evaluated nitrogen-removing bioretention systems for control of nutrients, organics, and solids in agricultural runoff. Pilot-scale experiments were conducted with bioretention systems incorporating aerobic nitrification and anoxic denitrification zones with sulfur or wood chips as denitrification substrates. Varying hydraulic loading rates (HLRs), influent concentrations, and wetting and drying periods were applied to the units during laboratory and two seasons of field tests with dairy farm runoff. Total N removal efficiencies greater than 88% were observed in both units with synthetic storm water. In first-season field tests, moderate removal efficiencies were observed for chemical oxygen demand (46%), suspended solids (69%), total phosphorous (TP) (66%), and total N (65%). During the second season, operational changes in the farm resulted in lower organic, solids, and nutrient loadings resulting in improved effluent quality, especially for suspended solids (81% removal) and total N (82% ...

Interhemispheric leakage of isotopically heavy nitrate in the eastern tropical Pacific during the last glacial period

We present new high-resolution N isotope records from the Gulf of Tehuantepec and the Nicaragua Basin spanning the last 50-70 ka. The Tehuantepec site is situated within the core of the north subtropical denitrification zone while the Nicaragua site is at the southern boundary. The delta N-15 record from Nicaragua shows an ``Antarctic'' timing similar to denitrification changes observed off Peru-Chile but is radically different from the northern records. We attribute this to the leakage of isotopically heavy nitrate from the South Pacific oxygen minimum zone (OMZ) into the Nicaragua Basin. The Nicaragua record leads the other eastern tropical North Pacific (ETNP) records by about 1000 years because denitrification peaks in the eastern tropical South Pacific (ETSP) before denitrification starts to increase in the Northern Hemisphere OMZ, i.e., during warming episodes in Antarctica. We find that the influence of the heavy nitrate leakage from the ETSP is still noticeable, although attenuated, in the Gulf of Tehuantepec record, particularly at the end of the Heinrich events, and tends to alter the recording of millennial timescale denitrification changes in the ETNP. This implies (1) that sedimentary delta N-15 records from the southern parts of the ETNP cannot be used straightforwardly as a proxy for local denitrification and (2) that denitrification history in the ETNP, like in the Arabian Sea, is synchronous with Greenland temperature changes. These observations reinforce the conclusion that on millennial timescales during the last ice age, denitrification in the ETNP is strongly influenced by climatic variations that originated in the highlatitude North Atlantic region, while commensurate changes in Southern Ocean hydrography more directly, and slightly earlier, affected oxygen concentrations in the ETSP. Furthermore, the delta N-15 records imply ongoing physical communication across the equator in the shallow subsurface continuously over the last 50-70 ka.

Estimation of Indirect Nitrous Oxide Emissions from a Shallow Aquifer in Northern Germany

Ground water is considered to be an important source for indirect N2O emissions. We investigated indirect N2O emissions from a shallow aquifer in Germany over a 1-yr period. Because N2O accumulated in considerable amounts in the surface ground water (mean, 52.86 microg N2O-N L(-1)) and corresponding fluxes were high (up to 34 microg N2O-N m(-2) h(-1)), it was hypothesized that significant indirect N2O emissions would occur via the vertical and the lateral emission pathway. Vertical N2O emissions were investigated by measuring N2O concentrations and calculating fluxes from the surface ground water to the unsaturated zone and at the soil surface. Lateral N2O fluxes were investigated by measuring ground water N2O and NO3- concentrations at five multilevel wells and at a waterworks well. Negligible amounts of N2O were emitted vertically into the unsaturated zone; most of it was convectively transported into the deeper autotrophic denitrification zone. Only a ground water level fall and rise triggered the emission of N2O (up to 3 microg N2O-N m(-2) h(-1)) into the unsaturated zone. Ground water-derived N2O was probably reduced during the upward diffusion, and soil surface emissions were governed by topsoil processes. Along the lateral pathway, N2O and NO3- concentrations decreased with increasing depth in the aquifer. Discharging ground water was almost free of N2O and NO3-, and indirect N2O emissions were small.

Evaluation of Noble Gas Recharge Temperatures in a Shallow Unconfined Aquifer

Water table temperatures inferred from dissolved noble gas concentrations (noble gas temperatures, NGT) are useful as a quantitative proxy for air temperature change since the last glacial maximum. Despite their importance in paleoclimate research, few studies have investigated the relationship between NGT and actual recharge temperatures in field settings. This study presents dissolved noble gas data from a shallow unconfined aquifer heavily impacted by agriculture. Considering samples unaffected by degassing, NGT calculated from common physically based interpretive gas dissolution models that correct measured noble gas concentrations for "excess air" agreed with measured water table temperatures (WTT). The ability to fit data to multiple interpretive models indicates that model goodness-of-fit does not necessarily mean that the model reflects actual gas dissolution processes. Although NGT are useful in that they reflect WTT, caution is recommended when using these interpretive models. There was no measurable difference in excess air characteristics (amount and degree of fractionation) between two recharge regimes studied (higher flux recharge primarily during spring and summer vs. continuous, low flux recharge). Approximately 20% of samples had dissolved gas concentrations below equilibrium concentration with respect to atmospheric pressure, indicating degassing. Geochemical and dissolved gas data indicate that saturated zone denitrification caused degassing by gas stripping. Modeling indicates that minor degassing (<10% DeltaNe) may cause underestimation of ground water recharge temperature by up to 2 degrees C. Such errors are problematic because degassing may not be apparent and degassed samples may be fit by a model with a high degree of certainty.

Modelling the N cascade in regional watersheds: The case study of the Seine, Somme and Scheldt rivers

The watersheds of the Seine, Somme and Scheldt rivers (France, Belgium, the Netherlands), flowing into the continental coastal zone of the English Channel and Southern North Sea, are among the regions of the world with the highest anthropogenic inputs of reactive nitrogen through fertilizer use, legume fixation and deposition of atmospheric nitrogen. They also represent examples of widely open systems, either exporting a large fraction of their N inputs under the form of agricultural products (case of the Seine basin) or importing high amounts of nitrogen as feed for livestock nutrition (case of the Scheldt basin), and delivering up to 2000 kg N km −2 yr −1 at river outlet into the sea. Taking these three watersheds as a case study, we review the different approaches developed so far for describing and predicting the fate of reactive nitrogen inputs to regional systems and its cascade from soils to sea. These approaches range from simple lumped input–output budget, to detailed process-based, spatially distributed models of nutrient transfers. The merits and the limits of these approaches are discussed. Their combination allows to establish a reasonably consistent budget for the three basins, emphasizing the various ‘retention’ terms linked to both landscape and in-stream processes, including storage in long residence time compartments (soil organic matter, vadose zone, aquifers, etc.), denitrification (in soil, riparian zones or river benthos) or sediment burial. Root-zone and riparian denitrification processes appear as major terms of landscape retention in all three investigated watersheds. Retention of nitrogen associated with collection and treatment of urban wastewater is also a major term in the two most populated watersheds.

Nitrate, nitrite, and nitrous oxide transformations in sediments along a salinity gradient in the Weser Estuary

The dynamics of the nitrogen intermediates N 2 O and NO 2 - in estuarine sediments were studied along a salinity gradient in the eutrophied Weser Estuary (Germany) using microsensors for N 2 O, NO 2 - , NO x - , and O 2 . During dark incubations in the laboratory of sediment samples from a freshwater site, a brackish water site with fluctuating salinity, and a marine site, the effects of environmental changes in nitrogen availability and salinity on microscale sediment dynamics were examined. Generally, sediment levels of intermediates were low: 1 to 25 μM NO 2 - and 0 to 8 μM N 2 O. However, significant variation was found in accumulation patterns and in the potential of the residing microbial community to control sediment releases of the intermediates. At fresh- and brackish water sites, NO 2 - production was found in the anoxic denitrification zone, and release from the sediments was effectively prevented by activity of nitrite oxidisers in oxic surface layers. In contrast, high rates of NO 2 - release occurred in marine sediment, where NO 2 - production was predominantly associated with incomplete nitrification in oxic layers. Similarly, stimulated partial nitrification due to NH 4 + addition led to NO 2 - liberation from brackish water sediment. Production of N 2 O was never observed in sediment from the brackish water site, which is naturally exposed to a daily regime of water column variations, but transient N 2 O accumulation was observed in the other sediments. The production of N 2 O could be induced by an abrupt change in either NO 3 - or salinity, and was found in anoxic or micro-oxic sediment layers. Because oxic sediment layers showed little or no potential for N 2 O consumption (in contrast to NO 2 - ) the accumulation of N 2 O always resulted in release from the sediment surface. Results demonstrate that changes in environmental parameters such as salinity and NO 3 - can trigger sediment production and release of NO 2 - and N 2 O, but further suggest that microbial sediment communities are highly adaptive and can become resistant towards intermediate release when regularly exposed to fluctuating conditions.

North Pacific-wide spreading of isotopically heavy nitrogen during the last deglaciation: Evidence from the western Pacific

Abstract. Sedimentary δ15 N records in two IMAGES cores (MD012404 and MD012403) retrieved from the Okinawa Trough (OT) in the western North Pacific reveal deglacial increases with two peaks occurring during the Bølling/Allerød and the Preboreal/early Holocene periods. These peaks are synchronous with previously reported δ15 N peaks in the Eastern Tropical North Pacific, although the amplitudes (from 3.8 to 5.8‰) are much smaller in the OT. Similar δ15 N values for the last glacial maximum and the late-Holocene observed by us at a site far from the present-day zones of water-column denitrification (WCD) indicate that the mean 15 N/14 N of nitrate in the upper ocean did not differ much between the two climate states. The accumulation rate of organic carbon and total sulfur content are used as indices of the local WCD potential. The results suggest that enhancement of global WCD rather than local denitrification should be responsible for the deglacial maxima of sedimentary δ15 N in the Okinawa Trough. Our data could provide additional constraints to better understand changes in nitrogen budget during the glacial to interglacial transition.

Riparian zone denitrification affects nitrogen flux through a tidal freshwater river

Tidal freshwater zones (TFZ) of coastal rivers link terrestrial watersheds to the ocean and are characterized by large, regularly inundated riparian zones. We investigated the effect of riparian denitrification on nitrogen flux in the TFZ Newport River, North Carolina (U.S.A.) by developing an empirical model of denitrification and parameterizing it using measured denitrification rates, sediment oxidation-reduction potential dynamics, and riparian topography. Denitrification rates were measured monthly in laboratory-incubated sediment cores by using a membrane inlet mass spectrometer to assess net water-borne N2 flux from the cores. Annual average rates of denitrification in three intertidal riparian habitats, emergent marsh, mudflat, and hardwood forest, were 1864, 1956, and 2018 μg m−2 h−1, respectively. Laboratory experiments and in-situ monitoring revealed that the temporal lag between tidal inundation and reduced, denitrifying conditions was 4–5 h. Field measurements and remotely sensed data showed that the inundated surface area during high tide was three times greater than that at low tide. By combining data on denitrification, oxidation-reduction potential, and topography, the model predicted that the daily denitrification flux constituted 2–15% of the daily riverine nitrate flux during most of the year and >100% during low discharge periods. Current regional and global nitrogen budgets thus may overestimate nitrogen delivery to the ocean by not accounting for the TFZ denitrification.

Denitrification Potential, Root Biomass, and Organic Matter in Degraded and Restored Urban Riparian Zones

Hydrologic changes associated with urbanization often lead to lower water tables and drier, more aerobic soils in riparian zones. These changes reduce the potential for denitrification, an anaerobic microbial process that converts nitrate, a common water pollutant, into nitrogen gas. In addition to oxygen, denitrification is controlled by soil organic matter and nitrate. Geomorphic stream restorations are common in urban areas, but their effects on riparian soil conditions and denitrification have not been evaluated. We measured root biomass, soil organic matter, and denitrification potential (anaerobic slurry assay) at four depths in duplicate degraded, restored, and reference riparian zones in the Baltimore, Maryland, U.S.A., metropolitan area. There were three main findings in this study. First, although reference sites were wet and had high soil organic matter, they had low levels of nitrate relative to degraded and restored sites and therefore there were few differences in denitrification potential among sites. Evaluations of riparian restorations that have nitrate removal by denitrification as a goal should consider the complex controls of this process and how they vary between sites. Second, all variables declined markedly with depth in the soil. Restorations that increase riparian water tables will thus foster interaction of groundwater nitrate with near-surface soils with higher denitrification potential. Third, we observed strong positive relationships between root biomass and soil organic matter and between soil organic matter and denitrification potential, which suggest that establishment of deep-rooted vegetation may be particularly important for increasing the depth of the active denitrification zone in restored riparian zones.

A comparative study of methanol as a supplementary carbon source for enhancing denitrification in primary and secondary anoxic zones

A comparative study on the use of methanol as a supplementary carbon source to enhance denitrification in primary and secondary anoxic zones is reported. Three lab-scale sequencing batch reactors (SBR) were operated to achieve nitrogen and carbon removal from domestic wastewater. Methanol was added to the primary anoxic period of the first SBR, and to the secondary anoxic period of the second SBR. No methanol was added to the third SBR, which served as a control. The extent of improvement on the denitrification performance was found to be dependent on the reactor configuration. Addition to the secondary anoxic period is more effective when very low effluent nitrate levels are to be achieved and hence requires a relatively large amount of methanol. Adding a small amount of methanol to the secondary anoxic period may cause nitrite accumulation, which does not improve overall nitrogen removal. In the latter case, methanol should be added to the primary anoxic period. The addition of methanol can also improve biological phosphorus removal by creating anaerobic conditions and increasing the availability of organic carbon in wastewater for polyphosphate accumulating organisms. This potentially provides a cost-effective approach to phosphorus removal from wastewater with a low carbon content. New fluorescence in situ hybridisation (FISH) probes targeting methanol-utilising denitrifiers were designed using stable isotope probing. Microbial structure analysis of the sludges using the new and existing FISH probes clearly showed that the addition of methanol stimulated the growth of specific methanol-utilizing denitrifiers, which improved the capability of sludge to use methanol and ethanol for denitrification, but reduced its capability to use wastewater COD for denitrification. Unlike acetate, long-term application of methanol has no negative impact on the settling properties of the sludge.

Anaerobic denitrification in fungi from the coastal marine sediments off Goa, India

Denitrification is a microbial process during which nitrate or nitrite is reduced under anaerobic condition to gaseous nitrogen. The Arabian Sea contains one of the major pelagic denitrification zones and in addition to this, denitrification also takes places along the continental shelf. Prokaryotic microorganisms were considered to be the only players in this process. However recent studies have shown that higher microeukaryotes such as fungi can also adapt to anaerobic mode of respiration and reduce nitrate to harmful green house gases such as NO and N 2O. In this study we examined the distribution and biomass of fungi in the sediments of the seasonal anoxic region off Goa from two stations. The sampling was carried out in five different periods from October 2005, when dissolved oxygen levels were near zero in bottom waters to March 2006. We isolated mycelial fungi, thraustochytrids and yeasts. Species of Aspergillus and thraustochytrids were dominant. Fungi were isolated under aerobic, as well as anaerobic conditions from different seasons. Four isolates were examined for their denitrification activity. Two cultures obtained from the anoxic sediments showed better growth under anaerobic condition than the other two cultures that were isolated from oxic sediments. Our preliminary results suggest that several species of fungi can grow under oxygen deficient conditions and participate in denitrification processes.

Oxygen minimum zones (OMZs) in the modern ocean

In the modern ocean, oxygen minimum zones (OMZs) are potential traces of a primitive ocean in which Archean bacteria lived and reduced chemical anomalies occurred. But OMZs are also keys to understanding the present unbalanced nitrogen cycle and the oceans’ role on atmospheric greenhouse control. OMZs are the main areas of nitrogen loss (as N 2, N 2O) to the atmosphere through denitrification and anammox, and could even indirectly mitigate the oceanic biological sequestration of CO 2. It was recently hypothesized that OMZs are going to spread in the coming decades as a consequence of global climate change. Despite an important OMZ role for the origin of marine life and for the biogeochemical cycles of carbon and nitrogen, there are some key questions on the structure of OMZs at a global scale. There is no agreement concerning the threshold in oxygen that defines an OMZ, and the extent of an OMZ is often evaluated by denitrification criteria which, at the same time, are O 2-dependent. Our work deals with the identification of each OMZ, the evaluation of its extent, volume and vertical structure, the determination of its seasonality or permanence and the comparison between OMZs and denitrification zones at a global scale. The co-existence in the OMZ of oxic (in its boundaries) and suboxic (even anoxic, in its core) conditions involves rather complex biogeochemical processes such as strong remineralization of the organic matter, removal of nitrate and release of nitrite. The quantitative OMZ analysis is focused on taking into account the whole water volume under the influence of an OMZ and adapted to the study of the specific low oxygen biogeochemical processes. A characterization of the entire structure for the main and most intense OMZs (O 2 < 20 μM reaching 1 μM in the core) is proposed based on a previously published CRIO criterion from the eastern South Pacific OMZ and including a large range of O 2 concentrations. Using the updated global WOA2005 O 2 climatology, the four known tropical OMZs in the open ocean have been described: the Eastern South Pacific and Eastern Tropical North Pacific, in the Pacific Ocean; the Arabian Sea and Bay of Bengal, in the Indian Ocean. Moreover, the Eastern Sub-Tropical North Pacific (25–52°N) has been identified as a lesser known permanent deep OMZ. Two additional seasonal OMZs at high latitude have also been identified: the West Bering Sea and the Gulf of Alaska. The total surface of the permanent OMZs is 30.4 millions of km 2 (∼8% of the total oceanic area), and the volume of the OMZ cores (10.3 millions of km 3) corresponds to a value ∼7 times higher than previous evaluations. The volume of the OMZ cores is about three times larger than that of the associated denitrification zone, here defined as NMZ (‘nitrate deficit or NDEF > 10 μM’ maximum zone). The larger OMZ, relative to the extent of denitrification zone, suggests that the unbalanced nitrogen cycle on the global scale could be more intense than previously recognized and that evaluation of the OMZ from denitrification could underestimate their extent.

Persistent Elevated Nitrate in a Riparian Zone Aquifer

Streamside vegetated buffer strips (riparian zones) are often assumed to be zones of ground water nitrate (NO3(-)) attenuation. At a site in southwestern Ontario (Zorra site), detailed monitoring revealed that elevated NO3(-) -N (4-93 mg L(-1)) persisted throughout a 100-m-wide riparian floodplain. Typical of riparian zones, the site has a soil zone of recent river alluvium that is organic carbon (OC) rich (36 +/- 16 g kg(-1)). This material is underlain by an older glacial outwash aquifer with a much lower OC content (2.3 +/- 2.5 g kg(-1). Examination of NO3(-), Cl(-), SO4(2-), and dissolved organic carbon (DOC) concentrations; N/Cl ratios; and NO3(-) isotopic composition (delta15N and delta18O) provides evidence of four distinct NO3(-) source zones within the riparian environment. Denitrification occurs but is incomplete and is restricted to a narrow interval located within ~0.5 m of the alluvium-aquifer contact and to one zone (poultry manure compost zone) where elevated DOC persists from the source. In older ground water close to the river discharge point, denitrification remains insufficient to substantially deplete NO3(-). Overall, denitrification related specifically to the riparian environment is limited at this site. The persistence of NO3(-) in the aquifer at this site is a consequence of its Pleistocene age and resulting low OC content, in contrast to recent fluvial sediments in modern agricultural terrain, which, even if permeable, usually have zones enriched in labile OC. Thus, sediment age and origin are additional factors that should be considered when assessing the potential for riparian zone denitrification.

Denitrification in the Arabian Sea: A 3D ecosystem modelling study

A three-dimensional hydrodynamic-ecosystem model was used to examine the factors determining the spatio-temporal distribution of denitrification in the Arabian Sea. The ecosystem model includes carbon and nitrogen as currencies, cycling of organic matter via detritus and dissolved organic matter, and both remineralization and denitrification as sinks for material exported below the euphotic zone. Model results captured the marked seasonality in plankton dynamics of the region, with characteristic blooms of chlorophyll in the coastal upwelling regions and central Arabian Sea during the southwest monsoon, and also in the northern Arabian Sea during the northeast monsoon as the mixed layer shoals. Predicted denitrification was 26.2 Tg N yr −1,the greatest seasonal contribution being during the northeast monsoon when primary production is co-located with the zone of anoxia. Detritus was the primary organic substrate consumed in denitrification (97%), with a small (3%) contribution by dissolved organic matter. Denitrification in the oxygen minimum zone was predicted to be fuelled almost entirely by organic matter supplied by particles sinking vertically from the euphotic zone above (0.73 mmol N m −2 d −1) rather than from lateral transport of organic matter from elsewhere in the Arabian Sea (less than 0.01 mmol N m −2 d −1). Analysis of the carbon budget in the zone of denitrification (north of 10°N and east of 55°E) indicates that the modelled vertical export flux of detritus, which is similar in magnitude to estimates from field data based on the 234Th method, is sufficient to account for measured bacterial production below the euphotic zone in the Arabian Sea.

Groundwater dynamics and channel activity as major controls of in-stream nitrate concentrations in a lowland catchment system?

Summary In-stream nitrogen concentrations of a small agricultural lowland study catchment in Saxony-Anhalt, Germany, show high seasonal dynamics. Field data lead to the hypothesis that these dynamics are controlled by groundwater level fluctuations and the associated falling dry and rewetting of intermittent channels and not by seasonal variations of soil nitrogen leaching processes. To test this hypothesis a hypothetical model experiment was carried out using MODFLOW and MT3DMS. We simulated steady state and transient flow and nitrate transport in a two-dimensional model transect, driven by average and monthly lysimeter data of recharge and nitrate leaching. Falling dry and rewetting of open channels was accounted for. The transient groundwater flow model can capture the supposed mechanisms quite well. For conservative transport, the resulting concentration changes are in the order of 25 mg/l NO 3 -N, for the reactive transport simulation, the concentration ranges was still in the order of 12 mg/l NO 3 -N. It can therefore be concluded, that the dynamics of groundwater flow considerably control the dynamics of in-stream nitrate concentrations. The results of the steady state simulation based on averaged inflows are not equivalent to the averaged outflows given by the transient simulation, reflected in different loads. In such a dynamic system the steady state flow approach is not an acceptable representation of the system. Observed data and simulations showed similar relations of in-stream nitrate concentrations to groundwater level and discharge. Minor differences were explained by the simplified two-dimensional modelling concept in contrast to a spatially distributed, three-dimensional real-world catchment system. In a real-world system further processes superpose the assumed hydrological controls, such as in-stream or hyporheic zone denitrification, contribution of tile drains and observed fertilization practice.

Optimization of the activated sludge anoxic reactor configuration as a means to control nutrient removal kinetically

Factors influencing the determination of optimum reactor configuration for activated sludge denitrification are investigated in this paper. A kinetic optimization method is presented to evaluate optimal pre- and post-denitrification bioreactor stages. Applying the method developed, simulation studies were carried out to investigate the impacts of the ratio of the influent readily biodegradable and slowly biodegradable substrates and the oxygen entering the denitrification zones on the optimal anoxic reactor configuration. In addition, the paper describes the effects of the slowly biodegradable substrate on the denitrification efficiency using external substrate dosing, and it demonstrates kinetic considerations concerning the hydrolysis process. It has been shown that as a function of the biodegradable substrate composition, the stage system design with three optimized reactor compartments can effectively increase reaction rates in the denitrification zones, and can provide flexibility for varying operation conditions.