This study investigated the details of the innate and Th1/Treg-type-associated host immune responses in Trichuris suis and Oesophagostomum dentatum mono- and co-infected pigs and invitro in stimulated porcine dendritic cell cultures. Forty-eight pigs were allocated into a 2-factorial design with two groups trickle-inoculated with 10 T.suis eggs/kg/day (Group T) or 20 O.dentatum L3/kg/day (O). Another group (OT) was infected with both parasites. Group C remained uninfected. Expression of innate and Th1/Treg-cell-associated genes in gut mucosa and associated lymph nodes was determined by qPCR at necropsy day 35 and 71. Gene expression showed suppressed/inhibited Th1 and Treg-type immune reactions, in accordance with previous findings of a predominant Th2-type immune response to both nematodes. The invitro part examined the production of TNF-α in porcine dendritic cells (DC) exposed to T.suis and/or O.dentatum excretory/secretory (E/S) products. Further, binding capacity and structure of E/S products were characterized. Glycan and lectin-binding capacity were generally lower in O.dentatum E/S products compared to T.suis which may explain the earlier found weaker Th2 response to the former. Surprisingly, O.dentatum E/S products induced a significant (P<0·0001) increase in TNF-α DC production, potentially indicating a new mode of helminth-host immune response interaction.