Demineralization Process Research Articles

ЭФФЕКТИВНОСТЬ ЭКЗОГЕННОЙ ПРОФИЛАКТИКИ КАРИЕСА ПРИ ПОМОЩИ ТРЕХКОМПОНЕНТНОГО КАЛЬЦИЙ-ФТОР-ФОСФАТ-СОДЕРЖАЩЕГО ГЕЛЯ У БЕРЕМЕННЫХ ЖЕНЩИН С РАЗЛИЧНЫМ УРОВНЕМ РЕЗИСТЕНТНОСТИ ЗУБНОЙ ЭМАЛИ

Subject. Dental prophylaxis in pregnant women is frequently ineffective, and the indicators to characterize the dental caries intensity, and its growth rate during pregnancy have been persisted at a fairly high level. The tooth tissues resistance degree to aggressive factors of the oral cavity may be considered to be the significant. The teeth resistance to caries is determined by morphological structure of the enamel. A three-component calcium-phosphate-fluorine-containing gel improves the quality characteristics of tooth enamel, which is confirmed by the data of the KOSRE-test (clinical determination of enamel remineralization rate) and the electrical conductivity of tooth enamel. The dynamic assessment of using the three-component calcium-phosphate-fluorine-containing gel effectiveness as a mean of preventing caries in pregnant women with different levels of tooth enamel resistance to demineralization processes has been quoted.
 Objectives. Evaluation of the effectiveness of caries prevention using the three-component calcium-phosphate-fluorine-containing gel in pregnant women with different levels of resistance.
 Methodology. Clinical examining and observing 126 pregnant women with different levels of resistance have been performed. As a means of caries prevention, the three-component calcium-phosphate-fluorine-containing gel with the ratio Ca:F:P = 2:1:1 was used.
 Results. After applying a three-component calcium-phosphate-fluorine-containing gel the tooth enamel electrical conductivity of women with different levels of resistance has significantly decreased in comparison with the initial values. According to the KPUP Index data any statistically significant increasing in new carious cavities hasn’t been noticed after a year after using the three-component calcium-phosphate-fluorine-containing gel. By the way a low degree of tooth enamel compliance to the acid influencing, and the statistically significant rate of artificial demineralization focus recovering have been noted after using the gel.
 Conclusion. The caries-prophylactic measures have been determined to have to be administered for pregnant women with taking into account the tooth resistance level, because the teeth enamel at pregnant women with different levels of resistance has dissimilar resistance to demineralization processes. 
 The three-component calcium-phosphate-fluorine-containing gel prescribed by using application is considered to be the versatile mean of caries prevention for pregnant women with different levels of resistance that has been confirmed by improving the electrical conductivity of tooth enamel to be the indicator of its mineralization, the data of the KOSRE-test, and the values of the DMFT Index (sum of all surfaces of permanent and milk teeth affected by caries, filled and removed) in observation dynamics.

Dentin, Dentin Graft, and Bone Graft: Microscopic and Spectroscopic Analysis

The use of the human dentin matrix could serve as an alternative to autologous, allogenic, and xenogeneic bone grafts. Since 1967, when the osteoinductive characteristics of autogenous demineralized dentin matrix were revealed, autologous tooth grafts have been advocated. The tooth is very similar to the bone and contains many growth factors. The purpose of the present study is to evaluate the similarities and differences between the three samples (dentin, demineralized dentin, and alveolar cortical bone) with the aim of demonstrating that the demineralized dentin can be considered in regenerative surgery as an alternative to the autologous bone. This in vitro study analyzed the biochemical characterizations of 11 dentin granules (Group A), 11 demineralized using the Tooth Transformer (Group B), and dentin granules and 11 cortical bone granules (Group C) using scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS) to evaluate mineral content. Atomic percentages of C (carbon), O (oxygen), Ca (calcium), and P (phosphorus) were individually analyzed and compared by the statistical t-test. The significant p-value (p < 0.05) between group A and group C indicated that these two groups were not significantly similar, while the non-significant result (p > 0.05) obtained between group B and group C indicated that these two groups are similar. The findings support that the hypothesis that the demineralization process can lead to the dentin being remarkably similar to the natural bone in terms of their surface chemical composition. The demineralized dentin can therefore be considered an alternative to the autologous bone in regenerative surgery.

Evaluation of Remineralization Potential of Natural Substances on Artificially Induced Carious Lesions in Primary Teeth: An In Vitro Study.

To evaluate the remineralizing potential of natural substances on artificially induced caries lesions in primary teeth. A total of 50 primary molar teeth were selected and subjected to a demineralization process. Then samples were randomly divided into five groups for the remineralization process. Group I-colophony, group II-5% sodium fluoride (NaF) + colophony, group III-grape seed extract (GSE) + colophony, group IV-5% NaF + colophony + 10% peptide, and group V- GSE + colophony + 10% peptide. All the groups were subjected to remineralization using a brushing stimulator for 3,000 cycles. Assessment was done using Vickers hardness testing machine for evaluating the enamel surface microhardness (SMH) and scanning electron microscopy-energy dispersive X-ray analysis (SEM-EDX) for evaluating the surface morphology and mineral content, before and after demineralization and after remineralization, the obtained data was analyzed statistically using IBM Statistical Package for the Social Sciences (SPSS) software version 25. The enamel microhardness results of this study revealed that remineralization of enamel was highest in group V (212.83 ± 64.416) and least in group II (137.83 ± 26.324) p-value of 0.038. SEM-EDX analysis revealed high calcium (Ca) and fluoride (F) content in groups II and IV, which was significant (p-value of 0.001) from other groups. Surface morphology evaluated with SEM revealed spherical globular agglomerates and scaffolding deposits on the enamel surface in groups III and V resembling the remineralization process. Grape seed extract (GSE) with colophony and peptide is a superior natural alternative to NaF. Colophony also exhibited remineralizing potential in primary enamel. Natural remineralizing agents like GSE, colophony, and its combination serves as a potential alternative to overcome the toxic effect on long-term usage of F. These natural substances can be applicable in clinical conditions by incorporating toothpaste and varnish, which can be used as an alternative or adjuvant to the topical application of F. Pooja V Ravi, Rajakumar S, Kavitha Ramar. Evaluation of Remineralization Potential of Natural Substances on Artificially Induced Carious Lesions in Primary Teeth: An In Vitro Study. Int J Clin Pediatr Dent 2023;16(2):244-250.

Hydrogel Preparation from Shrimp Shell-Based Chitosan: The Degree of Crosslinking and Swelling Study

Chitosan is a natural polymer derived from different starting materials such as fish scales, crab and shrimp shells. Due to the advantages like biocompatibility and biodegradability, chitosan has been widely used in hydrogel development. This current study aims to make chitosan from shrimp shells, synthesize hydrogel from chitosan, and observe the effect of various chitosan preparation treatments on the properties of the hydrogel. The preparation of chitosan was carried out through demineralization, deproteinization, and deacetylation process. HCl concentration during demineralization and NaOH concentration during deproteinization were varied (1; 1,5; 2) M and (1; 1,5; 2) M, respectively. Chitin deacetylation was conducted using 60% (w/v) of NaOH at the temperature of 90oC for 120 min, and chitosan was resulted. Chitosan based hydrogel was then synthesized with the addition of alginate and glutaraldehyde. The effect of HCl and NaOH concentrations during demineralization and deproteinization on the deacetylation degree of chitosan was observed. The effect of deacetylation degree of chitosan on the degree of crosslinking and swelling property of the hydrogel were also evaluated. Chitosan resulted from this study has the optimum degree of deacetylation at 57.28 %, resulting from demineralization by using HCl 2M and deproteinization with NaOH2 M. Higher deacetilation degree of chitosan causing the increase of the degree of cross-linking and decrease of the swelling capacity of the hydrogel. The highest degree of cross-linking is 78.85 %, and the swelling capacity is 47 %.

Olive Oil as a Transport Medium for Bioactive Molecules of Plants?-An In Situ Study.

(1) Caries and erosions still remain a challenge for preventive dentistry. Certain plant extracts have shown beneficial effects in preventive dentistry. The aim of this study was to evaluate the antibacterial, anti-adherent and erosion-protective properties of ellagic acid (EA) as a polyphenolic agent. The combination with olive oil was investigated additionally to verify a possible improved bioactive effect of EA. (2) An in situ study was carried out with six subjects. Individual splints were prepared with bovine enamel specimens. The splints were worn for 1 min (pellicle formation time). Thereafter, 10 min rinses were performed with EA in water/in oil. Bacterial adherence was evaluated by fluorescence microscopy (DAPI, ConA, BacLight) after an 8 h oral exposition time. Additionally, the splints were worn for 30 min to quantify demineralization processes. The ultrastructure of the pellicle was investigated after an oral exposure time of 2 h under a transmission electron microscope. Statistical analysis was performed by Kruskal-Wallis tests, Mann-Whitney U tests and Bonferroni-Holm correction. (3) Rinsing with EA led to a significant reduction of adherent vital and dead bacteria. The combination with olive oil did not improve these outcomes. The assessment of glucan structures after rinsing with EA in water showed significant effects. Significant differences were observed for both rinses in calcium release at pH 3.0. After rinsing with EA in oil, significantly less calcium was released compared to rinsing with EA in water (pH = 3.0). (4) Olive oil is not suitable as a transport medium for lipophilic polyphenols. EA has anti-adherent and antibacterial properties in situ. EA also shows erosion-protective effects, which can be enhanced in combination with olive oil depending on the pH value. Ellagic acid has a neutral pH and could be an opportunity in the treatment of specific patient groups (xerostomia or mucositis).

ENGINEERING A TENDON-BONE ENTHESIS-MIMICKING HYBRID MATRIX FOR REPAIR OF IRREPARABLE ROTATOR CUFF TEARS

Rotator cuff tears are common, with failure rates of up to 94% for large and massive tears1. For such tears, reattachment of the musculotendinous unit back to bone is problematic, and any possible tendon-bone repair heals through scar tissue rather than the specially adapted native enthesis. We aim to develop and characterise a novel soft-hard tissue connector device, specific to repairing/bridging the tendon-bone injury in significant rotator cuff tears, employing decellularised animal bone partially demineralised at one end for soft tissue continuation.Optimisation samples of 15×10×5mm3, trialled as separate cancellous and cortical bone samples, were cut from porcine femoral condyles and shafts, respectively. Samples underwent 1-week progressive stepwise decellularisation and a partial demineralisation process of half wax embedding and acid bathing. Characterisations were performed histologically for the presence/absence of cellular staining in both peripheral and central tissue areas (n=3 for each cortical/cancellous, test/PBS control and peripheral/central group), and with BioDent reference point indentation (RPI) for pre- and post-processing mechanical properties.Histology revealed absent cellular staining in peripheral and central cancellous samples, whilst reduced in cortical samples compared to controls. Cancellous samples decreased in wet mass after decellularisation by 45.3% (p&lt;0.001). RPI measurements associated with toughness (total indentation depth, indentation depth increase) and elasticity (1st cycle unloading slope) showed no consistent changes after decellularisation. X-rays confirmed half wax embedding provided predictable control of the mineralised-demineralised interface position.Initial optimisation trials show proof-of-concept of a soft-hard hybrid scaffold as an immune compatible xenograft for irreparable rotator cuff tears. Decellularisation did not appreciably affect mechanical properties, and further biological, structural and chemical characterisations are underway to assess validity before in vivo animal trials and potential clinical translation.

Analysis of Dental Enamel Remineralization: A Systematic Review of Technique Comparisons.

The demineralization process conditions the structure of the enamel and begins with a superficial decalcification procedure that makes the enamel surface porous and gives it a chalky appearance. White spot lesions (WSLs) are the first clinical sign that can be appreciated before caries evolves into cavitated lesions. The years of research have led to the testing of several remineralization techniques. This study's objective is to investigate and assess the various methods for remineralizing enamel. The dental enamel remineralization techniques have been evaluated. A literature search on PubMed, Scopus, and Web of Science was performed. After screening, identification, and eligibility processes 17 papers were selected for the qualitative analysis. This systematic review identified several materials that, whether used singly or in combination, can be effective in the process of remineralizing enamel. All methods have a potential for remineralization when they come into contact with tooth enamel surfaces that have early-stage caries (white spot lesions). From the studies conducted in the test, all of the substances used to which fluoride has been added contribute to remineralization. It is believed that by developing and researching new remineralization techniques, this process might develop even more successfully.

COMPARATIVE STUDY OF FLEXURAL STRENGTH OF PHOTOCOMPOSITE DENTAL ENAMEL SEALANTS

Background. Dental caries is a widespread worldwide disease of multifactorial genesis. The leading mechanism of its development is a long-term imbalance of the physiological balance between the inorganic component of the teeth hard tissues and the liquid biofilm formed on their surface, the predominance of the demineralization processes in hard tissues over the processes of remineralization. The use of dental enamel sealants has proven to be effective in preventing and reducing the intensity of dental caries. Modern bioactive polymer sealants for enamel contain such remineralizing agents as sodium fluoride, nanoamorphous calcium phosphate, beta-tricalcium phosphate, and particles of bioactive glass. For all dental composite filling materials, mechanical stability is one of the prerequisites for the long-term clinical success of restorations and fillings, and accordingly, temporary protective structures made of sealants.&#x0D; Aim: to compare the flexural strength and modulus of elasticity of three modern photocomposite enamel sealants.&#x0D; Materials and methods. In laboratory conditions for three modern photocomposite dental materials for sealing fissures and pits of enamel "Fissurit FX" (VOCO), "Clinpro™ Sealant" (3M™ ESPE™) and "Jen-Fissufil" (Jendental-Ukraine LLC) a flexural strength (three-point test) and elastic modulus were determined in accordance with the requirements of the international standard ISO 4049:2019. 6 samples of each material, polymerized and kept for a day in humid conditions, were examined.&#x0D; Results. The highest elastic modulus and flexural strength were in "Fissurit FX" – 5.17±0.80 GPa (M=5.00 GPa) and 130.07±7.75 MPa (M=127.81 MPa), respectively. In "Clinpro™ Sealant" the elastic modulus was 2.97±0.12 GPa (М=3.00 GPa), the flexural strength was 100.01±14.33 (М=96.73) MPa. In "Jen-Fissufil" the elastic modulus was 3.47±0.52 GPa (M=3.17 GPa), the flexural strength was 90.91±6.66 MPa (M=93.02 MPa). The studied indicators of all three materials exceeded the requirements of the international standard ISO 4049:2019.&#x0D; Conclusion. The performed studies showed high mechanical properties of all three dental sealants for enamel and that they have the potential to withstand long-term periodic masticatory load when functioning on the surface of teeth.

FORMULASI SEDIAAN OBAT KUMUR KOMBINASI EKSTRAK DAUN GAMBIR (Uncraina gambir (Hunter) Roxb) dan BIJI PINANG (Areca catechu L.) SEBAGAI ANTIBAKTERI

Bad breath (Halitosis) is one of the oral health problems that many people complain about after caries and periodontal disease. Plants that have the potential to be used as mouthwash preparations are gambier leaves and areca nut seeds. The content of phenol and catechin compounds has the ability as a bactericidal. Meanwhile, areca nut has the ability to inhibit the release of calcium ions in the tooth demineralization process stimulated by Streptococcus mutans. This study aims to determine the evaluation results of a combination of gambier leaf and areca nut mouthwash preparations with concentrations of 0.5%, 1%, and 1.5% with several physical parameters, namely organoleptic tests, pH tests, clarity tests, and viscosity tests. Physical stability testing was carried out on Day 0 and Day 7. The results of the organoleptic test are liquid, odorless, white and yellowish brown, sweet and bitter taste. ptest day 0 on controls, F1, F2, and F3, namely 6.2, 4.8, 4.8, 4.6, and day 7 on controls, F1, F2, and F3, namely 6.2, 4 ,7, 4,6, 4,4. Clarity test on day 0 and day 7 controls, F1, F2, and F3 were clear. Viscosity test day 0 on controls, F1, F2, and F3, namely 11.40 cPoises, 9.88 cPoises, 7,271 cPoises, 10.13 cPoises and day 7 on controls, F1, F2, and F3, namely 7,725 cPoises, 9,380 cPoises, 10,218 cPoises, 11 cPoises.

Effect of Remineralizing Agents on Shear Bond Strength of Orthodontic Brackets-In Vitro Study.

Orthodontic treatment can be effective only with the proper adhesion strength of the bonded elements on the teeth. The aim of the study was to analyze the influence of different remineralization products on the brackets (Evolve Low Profile Brackets 0.022 Roth prescription (DB Orthodontics Ltd., Silsden, England) shear bond strength (SBS)). In all, 40 teeth were investigated for this study; n = 30 demineralized (immersed in 0.1% citric acid for 30 min, twice a day, for 20 consecutive days) and n = 10 immersed only in artificial saliva. After the demineralization process, remineralization agents were applied to each group (n = 10): Group I: Elmex Sensitive professional® toothpaste (CP, Gaba GmbH, Witten, Germany) and GC MI Paste Plus® (GC, Leuven, Belgium), Group II: Elmex Sensitive professional® toothpaste (CP, Gaba GmbH, Germany) and GC Tooth Mousse® (Leuven, Belgium), Group III: Elmex Sensitive professional® toothpaste (CP, Gaba GmbH, Germany). For the teeth in control group C, Elmex Sensitive professional® toothpaste was used. SBS tests were performed by means of an advanced materials-testing machine that generated maximum load and tensile strength values. The data obtained underwent statistical analysis (ANOVA and Tuckey test) with a statistical threshold of p < 0.05. The SBS values were higher for group II (14.20 MPa) and I (10.36 MPa) and lower for group III (4.25 MPa) and C (4.11 MPa), with statistically significant differences between groups I and II when compared with groups III and C (p < 0.05). In conclusion, GC Tooth Mousse® and MI-Paste Plus® have no adverse effect on brackets SBS and are recommended to be used for enamel remineralization during orthodontic treatment.

The Effects of Demineralization on Reducing Ash Content in Corn and Soy Biomass with the Goal of Increasing Biofuel Quality

The increasing amount of residual waste presents several opportunities to use biomass as a renewable energy source. Agricultural biomass is a raw material with a high ash content, which can be a problem in any form of energy conversion. To obtain better quality biofuel, excess mineral matter must be removed. Demineralization is a simple form of mixing and washing biomass with various liquids to reduce ash content. Water, acetic acid, hydrochloric acid and nitric acid are common solvents used for this purpose. Ash is composed of different micro (Zn, Cu, Fe) and macro elements (Mg, Ca, K), which can have different consequences for the use of biomass for thermal energy. Different solvents have different effects on the individual elements, with inorganic acids having the greatest effect in demineralization processes, with a reduction in ash content of up to 80% for corn and about 99% for soybeans.

Effect of Demineralization on Gelatin Production from Grasshopper (Valanga nigricornis) as an Alternative of Halal Gelatin

Gelatin is a protein derivative product resulting from the collagen breakdown in a material. The protein content in dried grasshoppers, which are halal insects, reaches 40%. Hence, they have the potential to be produced as a substitute for non-halal gelatin. This study aims to determine the effect of various acid and base solvents and soaking time in the demineralization process on the characteristics of the gelatin. This study used a completely randomized design. The treatment of solvent types in the demineralization process used 6% HCl, 1% citric acid, 3% acetic acid, pineapple extract, and NaOH; while soaking times for treatment were 24 hours, 36 hours, and 48 hours. Each soaking time and solvent type in the demineralization process gave a significant effect on the yield value (P &lt; 0.05). Demineralization treatment using 6% HCl for 48 hours showed gelatin with the best yield than other treatments, i.e., gelatin with a moisture content of 9.93%, ash content of 1.11%, and pH 5. The characteristics of gelatin in this study are under SNI 06-3735 of 1995.

EFFECTS OF PROFESSIONAL ORAL HYGIENE AND TEETH WHITENING ON THE MICROELEMENT COMPOSITION OF ENAMEL

Aim: The objective of this study is to investigate the impact of professional teeth cleaning and the substances used in modern dentistry for whitening on the microelement composition of tooth enamel. Materials and Methods: To study the morphology and microelement composition of the enamel, scanning electron microscopy was performed using the MiraLM microscope equipped with a Schottky field emission electron gun from Tescan. Results: A comparative analysis between the areas subjected to mechanical cleaning and those where it was not applied revealed a significant difference in the research results, particularly in carbon, which changed from 25.16±1.04 to 32.02±1.8. An analysis of the enamel’s chemical composition before and after whitening revealed a decrease in carbon from 45.91±1.20 to 42.46±1.74. The change in phosphorus content was determined to be from 9.77±0.39 to 9.56±0.75. A decrease in calcium from 15.96±0.64 to 15.21±1.22 and magnesium from 0.07±0.01 to 0.01±0.01 was also observed. Conclusions: Professional dental hygiene does not have a direct impact on the microelement composition of enamel, such as the levels of calcium, phosphorus, fluoride, and other microelements. However, it can have an indirect and temporary influence due to the use of abrasive materials that affect dental deposits, pellicle, and the surface layer of enamel. Teeth whitening can affect the microelement composition of enamel, but these changes are mostly temporary and associated with processes of demineralization/ remineralization and oxygenation.

Comparative In Vitro Study Regarding the Effect of 2% and 6% Titanium Tetrafluoride on Demineralized Human Enamel

Background: Dental caries is the most common disease of the childhood and adulthood where the mineral contents of the enamel of the teeth begin to lose due to acids attacks by demineralization process. It can be prevented by application of fluoride that increased the mineral content of the enamel and made it highly resistant to acid demineralization. Objectives: This study was conducted to investigate the effects of titanium tetrafluoride (TiF4) in different concentrations and frequencies on surface microstructure and chemical structure of artificially demineralized enamel. Materials and Methods: The sample consisted of 35 premolar teeth: seven teeth randomly selected remained sound (five for energy dispersive spectroscopy analysis and two for scan electronic microscopy, whereas the remaining 28 teeth were divided into four treatment groups (n = 7): (1) control negative (deionized water), (2) control positive (acidulated phosphate fluoride gel), (3) 6% TiF4 solution once daily, (4) 2% TiF4 solution three times daily. Chemical structure and surface microstructure analyses were made for sound, after demineralization then after treatments. Results: The fluoride content was greater in 6% TiF4, whereas the calcium content was greater in control positive followed by 6% TiF4 then 2% TiF4 groups. Surface microstructure revealed uniform, smooth, glaze-like surface with no irregularities in 6% TiF4 while localized areas of glaze-like depositions were noted in 2% TiF4. Conclusions: The TiF4 showed better minerals contents and uniform surface in its higher concentration and the frequency of applications was not important as concentration.

A Physical Barrier Model for Preventing the Enamel Demineralization

The incidence and prevalence of dental caries in Indonesia has increased from year to year. The main cause is often associated with bad tooth brushing habits. Caries occurs because there is a process of demineralization of the surface of hard tooth tissues such as email due to the presence of acids produced by bacteria that metabolize the substrate. Coating the tooth surface with an acid-resistant material is an emerging idea for caries prevention in the future, Preliminary studies with extracted tooth models demonstrated that enamel surface coating was able to prevent demineralization through physical barrier. This method is possible as novel strategy of caries prevention on clinical level.

Treatment of brackish water inland desalination brine via antiscalant removal using persulfate photolysis

The combined persulfate photolysis and chemical demineralization process effectively treated brine for additional water recovery and mineral resource recovery. This technology has the potential to enhance the sustainability of desalination plants.

Shear bond strength fissure sealant based on glass ionomer after topical fluor application: a comparison between sodium fluoride and acidulated phosphate fluoride

Background: Application of fissure sealants to young permanent teeth is one of the preventive measures for caries in the field of dentistry. Fissure sealants based on glass ionomer cement (GIC) have the advantage of releasing fluoride. Sodium fluoride and acidulated phosphate fluoride (APF) are remineralization agents because they can increase remineralization by altering hydroxyapatite to fluorapatite and this in turn makes fluorine able to increase pH. Fluoride can prevent caries by increasing remineralization of the enamel that has just been demineralized by acids produced by plaque bacteria. The aim of this study is to compare the shear bond strength fissure sealant based on glass ionomer between sodium fluoride and APF topical fluor application Method: This research applied true experimental design with post test only control group design. The 21 maxillary permanent premolars were distributed into 3 groups, namely the control group (K1), the treatment group added 5% sodium fluoride (K2), and the treatment group added 1.23% APF (K3). Result: One Way ANOVA and post hoc LSD analysis test displayed no significant difference in each group. This could be due to the material used as a conditioner prior to the GIC application. The addition of fluoride ions will inhibit the demineralization process of the enamel produced by the application of conditioner. So it does not rule out that it will interfere with the attachment of the GIC material used as a fissure sealant in this research. Conclusion: There was no significant difference in shear bond strength of fissure sealant based on glass ionomer between sodium fluoride and acidulated phosphate fluoride topical application.

Formulation of Hand Sanitizer Gel from Chitosan Shell of Fresh Water Lobster (Cherax Quadricarinatus)

Certain aberrant disorders impair the operation of a portion or the entire body. Infectious diseases can be spread by touching a person's hands or after contact with inanimate objects contaminated with bacteria, which can cause a variety of ailments, including diarrhoea. Using hand sanitizer as the primary deterrent is one of the methods to avoid this transmission. This time, the primary element is a natural substance, such as chitosan. Chitosan can be extracted from the shells of freshwater crayfish and utilized as an antibacterial agent in hand sanitizer formulations. This project intends to produce chitosan from crab shells. Crustacean chitosan can be utilized as an active ingredient in hand sanitizer gel. The production of chitosan begins with the demineralization, deproteination, and deacetylation processes. The resulting chitosan was then used to make hand sanitizer gels with 1, 2, and 3% concentrations. Organoleptic, homogeneity, pH, dispersion, and viscosity tests were then conducted on the gel preparation. The results revealed that the hand sanitizer preparations subjected to organoleptic tests on F1, F2, and F3 did not exhibit any changes in odor, shape, or color; the homogeneity test revealed the absence of coarse grains; the pH obtained on average was within the skin pH range; and the spreadability test revealed that the average was in the range of 5-7 cm, indicating that the formula satisfies the specifications.

A Comparison of Yogurt and Probiotic Supplements Effects on the Hardness of Tooth Enamel: An In vitro Study

The demineralization of tooth enamel, which can be triggered by acidic foods and drinks at pH &lt; 5.5, can initiate dental caries. Yogurt and probiotic supplements are drinks that are often consumed to help digestive function and improve the immune system. However, both drinks have a low pH, thereby, a long time of consumption can trigger the demineralization process. This study aims to evaluate the enamel hardness after applying yogurt and probiotic supplements, which was carried out experimentally in a laboratory with a one-group post-test design. The sample used is 9 human maxillary premolars in each treatment group. The treatment group comprises the test material, such as yogurt and probiotic supplements, as well as one control group with artificial saliva. Dental samples were immersed in the test material for 96 hours, and the enamel hardness level was measured with a Micro Vickers Hardness tester. The T-paired test showed that soaking with yogurt does not significantly affect the hardness of the enamel, as indicated by a p-value of 0.016. However, the effect was significant in probiotic supplements, with p=0.004. The results showed that soaking with yogurt was not significantly different from probiotic supplements in reducing enamel hardness, as evidenced by the Post Hoc (LSD) test (p=0.464). Conclusively, yogurt decreased enamel hardness less than probiotic supplements.

Bio-phenolic compounds production through fast pyrolysis: Demineralizing olive pomace pretreatments

An original pathway to renewable production of phenol-rich bio-oil by fast pyrolysis of pretreated olive pomace (OP) was researched. However, inherent inorganic elements (especially alkali and alkaline earth metals) in biomass feedstocks largely modified the final bio-oil composition. To alter their catalytic activity in fast pyrolysis, different demineralisation processes (water, acid and alkali pretreatments) were employed. There was also an in-depth study of bio-oil phenolic compounds, analysing the oxygen-containing substituted groups linked to the benzene ring. From water leached samples, potassium was largely removed, enhancing the selective production of guaiacol (at room temperature) and syringol (at T = 90 ºC). Important differences were observed with HCl and HNO3 pretreatments regarding carboxylic acid and phenolic distribution. For pretreatment with NaOH, the sodium content in the sample was observed to increase to 83%. Thus, optimal phenolic selectivity (36.9%) was achieved for the alkali pretreated OP sample, where guaiacol (5.9%) and vinyl guaiacol (6.2%) yields increased and methyl guaiacol decreased. This study marks an efficient and clean pathway for converting agricultural waste into valuable phenolic enriched bio-oil. This could constitute a new way of obtaining these compounds by using renewable agriculture biomass.