Separation flow in a curved duct is a common phenomenon in engineering applications, and it highly contributes to the performance of fluid machinery. Accurate prediction of curved duct flows using the computational fluid dynamics method remains a challenge due to the limitations of turbulence modeling. Hence, the high-fidelity method of the delayed detached eddy simulation (DDES) approach is employed to simulate the U-duct flow with a Reynolds number of 105. The DDES results are compared with experimental data from the study by Monson et al. (1990) and analyzed in detail. The Q-criterion is defined to analyze the vortex structures and study the mechanism in the flow separation region. Discussions are made on turbulence characteristics, including turbulence energy spectra, helicity density, and turbulence anisotropy in the U-duct flow. Results indicate that the regions near the wall and within flow separation are highly anisotropic. The turbulence near the wall region is in a two-dimensional state, and the turbulence within the flow separation region is in a “rod-like” state.