Objective: To find out how well Saudi Arabian healthcare workers followed infection control guidelines and determine whether there is a any relationship between the biographic profile of healthcare staff and their degree of compliance with infection control. Methodology: This descriptive-correlation study was conducted on healthcare workers from different Saudi Arabian hospitals. The study used a three-point Likert scale, and the rating scales used were “3” to indicate always, “2” to indicate sometimes, while “1” was used to indicate never. Results: Of 180 respondents, 62.8% were young adults, 73.3% were males, 68.3% were doctoral degree holders, 66.1% were licensed medical doctors, and 39.4% were advanced beginners. Saudi healthcare workers had an overall level of “high compliance” (mean=2.29). Chi-square test of independence (r2) revealed a statistically significant relationship between compliance to infection control practices and the following variables: age (p=0.0100), gender (p=0.0177), educational attainment (p=0.0001), and profession (p<0.0001). Conclusion: In Saudi Arabia, healthcare staff generally follow infection control procedures. Moreover, biographic profiles were significantly associated with the degree of infection prevention practice compliance by healthcare staff.