Matrix Degradation Research Articles

3D printing of thick film NTC thermistor from preceramic polymer composites for ultra-high temperature measurement

Integrating thick/thin film sensors into component systems has emerged as a prevalent approach for monitoring in extreme environments. However, traditional vapor deposition methods face obstacles, including complex fabrication processes and the degradation of sensitive materials at extremely high temperatures. This work delineates the development of a polysilazane composite dual-layer thick-film Negative Temperature Coefficient (NTC) thermistor characterized by its suitability for extreme temperatures and robust bond strength achieved through an advanced near-net-shape printing methodology. High-temperature resistant La(Ca)CrO3/polysilazane films were printed as the sensitive layer, while a dense layer formed by Cr2O3/polysilazane was used as the protective layer. The bilayer structure resulted in a 2.5-fold increase in adhesion strength compared to the single-layer La(Ca)CrO3/polysilazane films. Experimental results indicate that the dual-layer thick-film NTC thermistor can be operated long-term at 1300 °C with a resistance drift rate of 0.9 %/h and survive short-term exposure to temperatures up to 1550 °C. As a proof of concept, this work applied 3D printing technology to fabricate a polysilazane composite dual-layer thick-film NTC thermistor on the surface of turbine blades and demonstrated its functionality under flame impingement at nearly 1300 °C. Such flexible 3D printing techniques pave the way for a new paradigm in manufacturing sensors capable of withstanding ultra-high temperatures.

Remarkable improvement in photocatalytic activity of NiO nanoparticles through Ag doping: A kinetics-mechanism & recyclability

Metal oxides are recognized as exemplary materials for photocatalytic dye degradation attributed to their unique characteristics such as tunable electronic structure, prolonged stability, and cost-effectiveness. However, the wide band gap inherent to most of these materials often limits their light absorption to the ultraviolet (UV) region. Consequently, the doping of noble metal nanoparticles into the metal oxide nanostructures has been proposed to significantly improve their light-harvesting ability. This enhancement is primarily ascribed to the localized surface plasmon resonance (LSPR) effect associated with noble metal nanoparticles, which facilitates more effective utilization of light. Thus, in this study, we synthesized silver-doped nickel oxide (Ag:NiO) with varying Ag concentrations (0, 1, 2, 4, 6 wt%) to evaluate their photocatalytic performance. The X-ray diffraction (XRD) spectra revealed that NiO possesses a cubic crystalline structure, and a peak shift to a higher angle was noted for Ag doping, indicative of compressive strain. Further analyses of morphological, vibrational, and absorption properties were conducted through the acquisition of scanning electron microscopy (SEM) images, Raman spectra and UV–Vis absorption spectra, respectively. The photocatalytic degradation of methylene blue (MB) and rhodamine B (RhB) dyes was assessed, revealing that NiO with 4.wt.% Ag doping exhibited enhanced photocatalytic activity, achieving 99% degradation of MB and 90% degradation of RhB. Notably, Ag doping significantly enhances the light absorption capabilities and modifies the electronic structure of the NiO, thereby improving its performance in photocatalytic dye degradation.

Ion migration mechanisms in the early stages of drying and degradation of oil paint films

The study of film-formation processes of oil paints has been extensively addressed over the last decade and the influence of metal ions in the drying and degradation stages of oil paints has been demonstrated. This research aimed to determine a suitable methodology for monitoring the early drying stages of selected commercial oil paint films and to gain an insight into the migration mechanisms of material degradation taking place between adjacent paint films, with special attention to the influence of the lead white. For this purpose, a hybrid approach was adopted to characterize the composition of the paint and highlight failure mechanisms in the paint films through a wide range of time. The methods included scribe tests, percentage weight variation (ΔW%), attenuated reflectance Fourier transform infrared spectrophotometry (ATR-FTIR), gas chromatography-mass spectrometry (GC-MS), and thermal analysis with differential scanning calorimetry (TG-DSC). The results show how metal ions interact with the oil binder and the pigment in the adjacent paint film: the transverse migration of lead white is shown to affect the reactivity of polyunsaturated triglycerides, increasing the rate of oxygen uptake and promoting the formation of radicals and bonds between polymer chains, depending on the pigment with which it interacts.

Encapsulation of Active Substances in Natural Polymer Coatings.

Already used in the food, pharmaceutical, cosmetic, and agrochemical industries, encapsulation is a strategy used to protect active ingredients from external degradation factors and to control their release kinetics. Various encapsulation techniques have been studied, both to optimise the level of protection with respect to the nature of the aggressor and to favour a release mechanism between diffusion of the active compounds and degradation of the barrier material. Biopolymers are of particular interest as wall materials because of their biocompatibility, biodegradability, and non-toxicity. By forming a stable hydrogel around the drug, they provide a 'smart' barrier whose behaviour can change in response to environmental conditions. After a comprehensive description of the concept of encapsulation and the main technologies used to achieve encapsulation, including micro- and nano-gels, the mechanisms of controlled release of active compounds are presented. A panorama of natural polymers as wall materials is then presented, highlighting the main results associated with each polymer and attempting to identify the most cost-effective and suitable methods in terms of the encapsulated drug.

New Insight into Bulk Structural Degradation of High-Voltage LiCoO2 at 4.55 V.

The aggravated mechanical and structural degradation of layered oxide cathode materials upon high-voltage charging invariably causes fast capacity fading, but the underlying degradation mechanisms remain elusive. Here we report a new type of mechanical degradation through the formation of a kink band in a Mg and Ti co-doped LiCoO2 cathode charged to 4.55 V (vs Li/Li+). The local stress accommodated by the kink band can impede crack propagation, improving the structural integrity in a highly delithiated state. Additionally, machine-learning-aided atomic-resolution imaging reveals that the formation of kink bands is often accompanied by the transformation from the O3 to O1 phase, which is energetically favorable as demonstrated by first-principles calculations. Our results provide new insights into the mechanical degradation mechanism of high-voltage LiCoO2 and the coupling between electrochemically triggered mechanical failures and structural transition, which may provide valuable guidance for enhancing the electrochemical performance of high-voltage layered oxide cathode materials for lithium-ion batteries.

Myricetin Restores Autophagy to Attenuate Lumbar Intervertebral Disk Degeneration Via Negative Regulation of the JAK2/STAT3 Pathway.

Autophagy is a critical player in lumbar intervertebral disk degeneration (IDD), and autophagy activation has been suggested to prevent the apoptosis of nucleus pulposus cells (NPCs). Myricetin has anti-cancer, anti-inflammatory, and antioxidant potentials and can activate autophagy. Thus, this study focused on the roles and mechanisms of myricetin in IDD. A puncture-induced rat IDD model was established and intraperitoneally injected with 20-mg/kg/day myricetin. Histopathological changes of intervertebral disks (IVDs) were assessed by hematoxylin and eosin staining and Safranin O/Fast Green staining. The isolated NPCs from IVDs of healthy rats were stimulated with IL-1β to mimic IDD-like conditions. The roles of myricetin in cell apoptosis, extracellular matrix (ECM) degradation, autophagy repression, and the JAK2/STAT3 pathway activation were examined by cell counting kit-8, flow cytometry, western blotting, real-time quantitative polymerase chain reaction, and immunofluorescence staining. Myricetin treatment attenuated the apoptosis and ECM degradation, and enhanced autophagy in the IL-1β-treated NPCs, whereas the myricetin-mediated protection was limited by autophagy inhibition. Mechanistically, myricetin activated autophagy through blocking the JAK2/STAT3 signaling. In vivo experiments revealed that intraperitoneal injection of myricetin activated NPC autophagy to relieve puncture injury in rats. Myricetin prevents IDD by attenuating NPC apoptosis and ECM degradation through blocking the JAK2/STAT3 pathway to enhance autophagy.

Peridynamics thermomechanical coupling simulation of damage in engineered cementitious composite-concrete bonding specimens under high temperature

The current application of Peridynamics (PD) models in addressing thermally induced failures within Engineered Cementitious Composites (ECC) and concrete bonding specimens is relatively limited. This study introduces a novel semi-discrete PD model for ECC and a random heterogeneous PD model for concrete. These models are grounded in the microscopic constitutive relationships between ECC and concrete materials and incorporate the strain softening behavior of cement-based materials. The effectiveness of the interface model is evaluated through splitting tensile tests on ECC-concrete bonding specimens, employing a series model alongside PD surface effects for material interface correction. Considering the performance degradation of materials at elevated temperatures, a PD thermo-mechanical coupling model, which integrates PD thermal diffusion and motion equations, is proposed to analyze the failure of ECC and concrete under high thermal loads. The validity of both the thermo-mechanical coupling and concrete heterogeneity models is confirmed via thermal cracking tests on heated concrete columns subjected to drilling. Lastly, considering the dynamic response of fire, the Navier-Stokes equation are reformulated into a non-local form utilizing the Peridynamic differential operator (PDDO) to simulate fire environment. A thermo-fluid-mechanical coupling model is developed, and applied to analyze ECC and concrete bonding specimens’ failure behaviors under fire scenarios.

Features of behavior and morphological stability of the N-doped PtCu/C catalyst

A high-performance electrocatalyst based on bimetallic platinum-containing nanoparticles for PEMFCs has been presented. The combination of bimetallic nanoparticles and an N-doped carbon support ensures high ORR activity and stability of the catalyst. The single-reactor method to obtain the material and its higher functional characteristics allow considering the resulting PtCu/C-N electrocatalyst as a promising commercial product. An extended study of morphological characteristics has been conducted to understand the processes of evolution for this material. The in-situ IL-TEM research has allowed studying in detail the degradation process for the material during high-temperature treatment by analyzing changes in the type of histograms of the nanoparticles size distribution and testing the method for calculating the number of their intersections to assess the dynamics of the material degradation.

A computational approach to integrate three-dimensional peridynamics and two-dimensional higher-order classical elasticity theory for fracture analysis

AbstractAs a nonlocal alternative of classical continuum theory, peridynamics (PD) is mathematically compatible to discontinuities, making it particularly attractive for failure prediction. The PD theory on the other side can be computationally demanding due to its nonlocal interactions. A coupling between PD and refined higher-order finite element method (FEM) integrates their salient features. The present study proposes a computational approach to couple three-dimensional peridynamics with two-dimensional higher-order finite elements based on classical elasticity. The bond-based PD modeling is considered in a region where damage might appear while refined finite element modeling is used for the remaining region. The refined finite elements employed in this study are based on the 2D Carrera Unified Formulation (CUF), which provides 3D-like accuracy with optimized computational efficiency. The coupling between PD and FEM is achieved through the Lagrange multiplier method which permits physical consistency and compatibility at the interface domain. An adaptive convergence check algorithm is also proposed to achieve predetermined accuracy in the solution with minimum computational effort. Simulations of quasi-static tension tests, wedge splitting tests and L-plate cracking tests are carried out for verification. In-depth analysis shows that the present approach can reproduce the linear deformation, material degradation and crack propagation in an effective way.

Potential of MMP-2 and MMP-9 Gelatinase Blockade as a Therapeutic Strategy in Fibrosarcoma Treatment: A Decadal Review

Fibrosarcoma represents a significant challenge in oncology, characterized by high invasiveness and a poor prognosis. Gelatinases, particularly matrix metalloproteinases MMP-2 and MMP-9, play a pivotal role in the degradation of the extracellular matrix, facilitating tumor invasion and metastasis. Inhibiting these enzymes has emerged as a promising therapeutic strategy. This review evaluates the progress in the development and therapeutic potential of gelatinase inhibitors as treatments for fibrosarcoma over the last decade, highlighting molecular mechanisms and future directions. A comprehensive literature review was conducted, focusing on studies published from 2013 to 2023. Research articles and review papers relevant to gelatinase inhibition and fibrosarcoma were examined to assess the efficacy and mechanisms of gelatinase inhibitors. Gelatinase inhibitors have shown the potential to reduce tumor progression, invasion, and metastasis in fibrosarcoma. Clinical trials, although limited, have indicated that these inhibitors can be effectively integrated into existing therapeutic regimens, offering a reduction in metastatic spread and potentially improving patient survival rates. Mechanistic studies suggest that the inhibition of MMP-2 and MMP-9 disrupts critical pathways involved in tumor growth and cell invasion. Gelatinase inhibition represents a viable and promising approach to fibrosarcoma treatment. Future research should focus on developing more specific inhibitors, understanding long-term outcomes, and integrating gelatinase inhibition into multimodal treatment strategies to enhance efficacy.

Using Data Science Tools to Reveal and Understand Subtle Relationships of Inhibitor Structure in Frontal Ring-Opening Metathesis Polymerization.

The rate of frontal ring-opening metathesis polymerization (FROMP) using the Grubbs generation II catalyst is impacted by both the concentration and choice of monomers and inhibitors, usually organophosphorus derivatives. Herein we report a data-science-driven workflow to evaluate how these factors impact both the rate of FROMP and how long the formulation of the mixture is stable (pot life). Using this workflow, we built a classification model using a single-node decision tree to determine how a simple phosphine structural descriptor (Vbur-near) can bin long versus short pot life. Additionally, we applied a nonlinear kernel ridge regression model to predict how the inhibitor and selection/concentration of comonomers impact the FROMP rate. The analysis provides selection criteria for material network structures that span from highly cross-linked thermosets to non-cross-linked thermoplastics as well as degradable and nondegradable materials.

Challenges and Pitfalls of Research Designs Involving Magnesium-Based Biomaterials: An Overview.

Magnesium-based biomaterials hold remarkable promise for various clinical applications, offering advantages such as reduced stress-shielding and enhanced bone strengthening and vascular remodeling compared to traditional materials. However, ensuring the quality of preclinical research is crucial for the development of these implants. To achieve implant success, an understanding of the cellular responses post-implantation, proper model selection, and good study design are crucial. There are several challenges to reaching a safe and effective translation of laboratory findings into clinical practice. The utilization of Mg-based biomedical devices eliminates the need for biomaterial removal surgery post-healing and mitigates adverse effects associated with permanent biomaterial implantation. However, the high corrosion rate of Mg-based implants poses challenges such as unexpected degradation, structural failure, hydrogen evolution, alkalization, and cytotoxicity. The biocompatibility and degradability of materials based on magnesium have been studied by many researchers in vitro; however, evaluations addressing the impact of the material in vivo still need to be improved. Several animal models, including rats, rabbits, dogs, and pigs, have been explored to assess the potential of magnesium-based materials. Moreover, strategies such as alloying and coating have been identified to enhance the degradation rate of magnesium-based materials in vivo to transform these challenges into opportunities. This review aims to explore the utilization of Mg implants across various biomedical applications within cellular (in vitro) and animal (in vivo) models.

Melatonin Delays Arthritis Inflammation and Reduces Cartilage Matrix Degradation through the SIRT1-Mediated NF-κB/Nrf2/TGF-β/BMPs Pathway.

Cartilage, a flexible and smooth connective tissue that envelops the surfaces of synovial joints, relies on chondrocytes for extracellular matrix (ECM) production and the maintenance of its structural and functional integrity. Melatonin (MT), renowned for its anti-inflammatory and antioxidant properties, holds the potential to modulate cartilage regeneration and degradation. Therefore, the present study was devoted to elucidating the mechanism of MT on chondrocytes. The in vivo experiment consisted of three groups: Sham (only the skin tissue was incised), Model (using the anterior cruciate ligament transection (ACLT) method), and MT (30 mg/kg), with sample extraction following 12 weeks of administration. Pathological alterations in articular cartilage, synovium, and subchondral bone were evaluated using Safranin O-fast green staining. Immunohistochemistry (ICH) analysis was employed to assess the expression of matrix degradation-related markers. The levels of serum cytokines were quantified via Enzyme-linked immunosorbent assay (ELISA) assays. In in vitro experiments, primary chondrocytes were divided into Control, Model, MT, negative control, and inhibitor groups. Western blotting (WB) and Quantitative RT-PCR (q-PCR) were used to detect Silent information regulator transcript-1 (SIRT1)/Nuclear factor kappa-B (NF-κB)/Nuclear factor erythroid-2-related factor 2 (Nrf2)/Transforming growth factor-beta (TGF-β)/Bone morphogenetic proteins (BMPs)-related indicators. Immunofluorescence (IF) analysis was employed to examine the status of type II collagen (COL2A1), SIRT1, phosphorylated NF-κB p65 (p-p65), and phosphorylated mothers against decapentaplegic homolog 2 (p-Smad2). In vivo results revealed that the MT group exhibited a relatively smooth cartilage surface, modest chondrocyte loss, mild synovial hyperplasia, and increased subchondral bone thickness. ICH results showed that MT downregulated the expression of components related to matrix degradation. ELISA results showed that MT reduced serum inflammatory cytokine levels. In vitro experiments confirmed that MT upregulated the expression of SIRT1/Nrf2/TGF-β/BMPs while inhibiting the NF-κB pathway and matrix degradation-related components. The introduction of the SIRT1 inhibitor Selisistat (EX527) reversed the effects of MT. Together, these findings suggest that MT has the potential to ameliorate inflammation, inhibit the release of matrix-degrading enzymes, and improve the cartilage condition. This study provides a new theoretical basis for understanding the role of MT in decelerating cartilage degradation and promoting chondrocyte repair in in vivo and in vitro cultured chondrocytes.

Synthesis of novel bio-based and degradable polyurethanes using lignin oligomers

Bio-based and degradable materials were proposed to challenge the major problem of plastic disposal in the environment. In this context, polyurethane production was re-evaluated, encouraging the search for replacing both petroleum components and highly toxic species. A novel synthesis route is explored in this work, aimed to produce degradable lignin-based polyurethanes. Oligomers from steam-exploded lignin were extracted and used with ε-caprolactone (ε-CL) to generate a fully bio-based pre-polymer (oligo-grafted-poly(ε-CL)), exploiting ring-opening polymerization. We have demonstrated that tuning the main reaction parameters, such as ε-CL:oligomer and catalyst:ε-CL mass ratios, and reaction time, it is possible to obtain different pre-polymers enabling the synthesis of bio-based polyurethanes with variable physicochemical properties. In particular, the oligomeric content modulates the thermal and mechanical properties of the polymer (melting point range: 54–62 °C; Young modulus range: 3–7 kPa) and enhances the degradability (up to 13 % wt, in acid environment), highlighting the potential of the material for possible applications.

Sintered and unsintered pressed fly ash geopolymer: A comprehensive study on structural transformation in nitric and sulfuric acid

Acidic attacks contribute to the degradation of cementitious materials, diminishing the structural service life and increasing the requirement for maintenance of the structure. To address the limited understanding of the impact of the sintering process on the acid resistance of pressed geopolymer, an investigation and comparison of the acid resistance of room-cured (RPG) and sintered (SPG) pressed geopolymer was performed. Specimens were immersed in 3 % and 8 % nitric (HNO3) and sulfuric (H2SO4) acids for 7 and 28 days. Despite the higher sorptivity, SPG demonstrated better mechanical strength retention than that of RPG. Specifically, the compressive strength of SPG after 3 % of HNO3 immersion for 28 days increased (+14.2 %), surpassing the control specimen, while RPG experienced a 14.5 % strength drop. The strength increment in SPG was due to the further geopolymerization during acid immersion. In RPG, a new crystalline phase of NaNO3 was observed after immersion in 8 % HNO3 for 28 days. In contrast, SPG showed no evidence of NaNO3 formation, indicating lower reactivity with HNO3 compared to RPG. Additionally, both RPG and SPG exhibited gypsum formation after immersion in H2SO4. The presence of gypsum induced crack formation in RPG, whereas SPG, with its intensive cross-linked structure, effectively compensated for gypsum expansion, preventing crack formation. This finding is crucial for practical applications where exposure to aggressive acids is a concern, as it provides a method to enhance the acid resistance of geopolymer structures.

Mechanism of self-supporting montmorillonite composite material for bio-enhanced degradation of chlorotetracycline: Electron transfer and microbial response

The efficient degradation of antibiotics holds significant implications for mitigating environmental pollution. This study synthesized a montmorillonite chitosan composite material (MMT-CS) using the gel template method. Subsequently, a bio-enhanced reactor was constructed to facilitate the degradation of chlorotetracycline (CTC). The addition of MMT-CS composite material enables the degradation of different concentrations of CTC. MMT-CS, a conductive carrier, effectively promotes microbial adhesion and boosts the metabolic activity of functional microorganisms. Additionally, it facilitates the maintenance of microbial activity under CTC pressure by promoting the secretion of extracellular polymeric substances, increasing critical enzyme activity, and enhancing the electron transfer capacity within the system. In this MMT-CS bio-enhanced process, Paracoccus (11.4%) and Bacillus (3.9%) are utilized as essential bacteria genes. The results of metabolic pathways prediction indicated significant enhancements in membrane-transport, nucleotide-metabolism, replication-repair, and lipid-metabolism. Thus, the developed self-supporting MMT-CS bio-enhanced process ensured the stability of the system during the removal of antibiotics.

Ultra-processing should be understood as a holistic issue, from food matrix, to dietary patterns, food scoring, and food systems.

The ultra-processed food (UPF) concept first emerged 15 years ago, and is now studied worldwide in different contexts, for example, human health, food behavior, socio-economic, food consumption, food scoring, and food system sustainability. Briefly, UPFs are defined as containing at least one marker of ultra-processing (MUP). MUPs are (1) cosmetic additives, (2) aromas, (3) some highly processed carbohydrates, proteins, fats, and/or fiber, and (4) drastic processes directly applied to food such as extrusion cooking or puffing. The first three categories of MUPs are on the food packaging in the list of ingredients, and are extracted, then purified, from raw foods or coming from artificial syntheses, leading to a-matrix/a-cellular compounds. Therefore, the core paradigm to define MUP is extreme food matrix degradation, and for UPF, matrix artificialization. Besides, UPFs are more than just junk food, encompassing numerous industrialized foods, falsely presented as healthy, for example, animal-based food analogs, but also organic, vegan, gluten-free, micronutrient-enriched, and/or light foods. In this way, UPFs are "high-quality junk foods." Otherwise, UPF being a holistic and indivisible concept by essence, we propose in this review to analyze ultra-processing at four holistic levels corresponding to four important scientific issues: the food matrix, the dietary pattern, food system, and food scoring. We reached the main conclusion that UPFs should be first studied with a holistic and scientifically based approach, not a reductionist one. Otherwise, we take the risk of performing greenwashing and create still more new health threats at a global level.

Reflections on the Decay Mechanisms of Half-Timbered Walls in Traditional Spanish Architecture: Statistical Analysis of Material and Structural Damage

Knowledge on the state of conservation and vulnerability of traditional techniques when faced with the most common degradation phenomena is vital in order to propose the most suitable conservation and maintenance actions. This article presents the systematic review of 1218 half-timbered walls found throughout Spain, enabling the identification of a total of 27 material lesions, classified by atmospheric, biological or anthropic origin, and 9 structural lesions due to stress or excessive deformation. Their qualitative and quantitative analysis has focused on the frequency of the individual lesions and the possible correlation with different constructive characteristics, such as the materials used, the geometry of the framework and the presence of plinths, eaves and protective rendering. Almost the entire sample presents some degree of material degradation, mostly atmospheric lesions of limited severity, such as superficial atmospheric erosion and chromatic alteration and dehydration of the timber. In terms of structural lesions, half-timbered walls are seen to be more vulnerable to this type of deformation. Considering the risk of loss affecting all traditional architecture, it becomes particularly important to promote the continued maintenance of half-timbered walls in order to reduce the influence of material lesions caused by atmospheric agents. Subsequently, suitable criteria for intervention are established in order to reduce the effect of anthropic lesions and structural degradation phenomena, particularly linked to a lack of maintenance and modifications of anthropic origin.

The new kids on the block: RNA-binding proteins regulate autophagy in disease.

Mammalian autophagy is a highly regulated and conserved cellular homeostatic process. Its existence allows the degradation of self-components to mediate cell survival in different stress conditions. Autophagy is involved in the regulation of cellular metabolic needs, protecting the cell or tissue from starvation through the degradation and recycling of cytoplasmic materials and organelles to basic molecular building blocks. It also plays a critical role in eliminating damaged or harmful proteins, organelles, and intracellular pathogens. Thus, a deterioration of the process may result in pathological conditions, such as aging-associated disorders and cancer. Understanding the crucial role of autophagy in maintaining the normal physiological function of cells, tissue, or organs has led to copious and expansive research regarding the regulation of this process. So far, most of the research has revolved around transcriptional and post-translational regulation. Here, we discuss the regulation of autophagy-related (ATG) mRNA transcripts by RNA-binding proteins (RBPs). This analysis focuses on how RBPs modulate autophagy in disease. A deeper understanding of the involvement of RBPs in autophagy can facilitate further research and treatment of a variety of human diseases.

HIF-1α activation impairs dendrites formation and elongation in osteocytogenesis

Osteocytes are terminally differentiated cells derived from osteoblasts and are deeply embedded within the bone matrix. They play a critical role in bone remodeling by generating a lacuno-canalicular network (LCN) and controlling the transport of nutrients. Due to the absence of blood vessels within the bone matrix, it is widely believed that osteocytes develop in a hypoxic environment. However, the mechanisms of osteocytogenesis and the role of oxygen sensing in this process remain unclear. Hypoxia-inducible factors (HIFs) are major transcriptional factors involved in oxygen sensing. Previous studies have shown that accumulation of HIFs in osteoblasts leads to abnormal bone remodeling, potentially linked with the alterations of the LCN network. Specifically, HIF-1α is hypothesized to play a more significant role in regulating bone remodeling compared to HIF-2α. Therefore, we investigated the functions of HIF-1α in dendrite formation and the establishment of the LCN network during osteocytogenesis. Immunostaining and scanning electron microscopy revealed that the E11 protein aggregates to form a ring structure that defines the site for dendrite initiation. This process is followed by activation of the ERM/RhoA pathway and recruitment of matrix metalloproteinase 14 (MMP14) to facilitate extracellular matrix degradation, enabling dendrite elongation. However, both hypoxic treatment and overexpression of HIF-1α impair ring formation, resulting in reduced ERM/RhoA activity and decreased matrix degradation capability. These findings suggest that abnormal HIF-1α activity in local areas could contribute to impaired LCN network formation and abnormal bone remodeling observed in bone diseases such as osteopenia and aging.