Due to high strength, toughness and durability, ultra-high-performance concrete (UHPC) can be applied in replacing the cracked concrete slab in the negative moment region of newly built steel-ordinary concrete (OC) continuous composite beams, as well as in repairing old composite beams. To investigate the failure mode, crack propagation, stiffness degradation and bearing capacity of the partial steel-UHPC continuous composite beam, a test model with an UHPC slab over the negative moment region were designed and fabricated to compare with a steel-OC model with the same conditions. Similar failure mode was observed in the loading test of two models. Nevertheless, the maximum crack width in the UHPC slab is only 0.17mm, much smaller than that in the OC slab. Compared with the steel-OC composite beam, vertical deflection of the partial steel-UHPC composite beam decreases by 32.7%, and its cracking load and ultimate bearing capacity increase by 50% and 6.4%, respectively. The results show that the substitution of the OC with the UHPC in the negative bending moment area significantly improves the crack resistance, delays the crack propagation, increases the bending stiffness, and improves the working performance of composite beams with cracks, displaying good advantages in economy and in reality. Parametric analysis demonstrates that the height of steel beam is the most important factor, and cross section of the steel beam is suggested to be given priority in design so as to take advantages of partial steel-UHPC composite beams. The research also provides an option for the repair of old concrete slabs in steel-OC continuous composite beams.