Defense System Research Articles

A widespread phage-encoded kinase enables evasion of multiple host antiphage defence systems.

DNA degradation (Dnd) is a widespread bacterial antiphage defence system that relies on DNA phosphorothioate (PT) modification for self/non-self discrimination and subsequent degradation of unmodified DNA. Phages employ counterstrategies to evade host immunity, but anti-Dnd immunity has not been characterized. Here we report an immune evasion protein encoded by the Salmonella phage JSS1 that contributes to subverting Dnd and other defence systems. Using quantitative proteomic and phosphoproteomic analyses, we show that the protein JSS1_004 employs N-terminal Ser/Thr/Tyr protein kinase activity to catalyse the multisite phosphorylation of host DndFGH. Notably, JSS1_004 also phosphorylates other bacterial immune systems to varying degrees, including CRISPR‒Cas, QatABCD, SIR2+HerA and DUF4297+HerA. Given that JSS1_004 and its homologues are widespread in phylogenetically diverse phages, we suggest that this strategy constitutes a family of immune evasion proteins that increases the chances of phage proliferation even when a host deploys multiple defence systems.

Türkiye’s Relations with/within NATO: The Ontological Security Dilemmas of Türkiye’s Transatlantic Identity

Since 1952, Türkiye’s Transatlantic identity has been largely shaped by its NATO membership and interactions with the Allies. During the Cold War, Türkiye aligned its national interests and security concerns with NATO, making its membership a central element of its identity. However, starting in the 2000s, Türkiye shifted toward a more independent foreign policy, influenced by its new activism and regional ambitions, particularly in the Middle East. This shift has caused tensions with NATO, especially considering Türkiye’s military interventions in Syria, its purchase of the S-400 missile defense system from Russia, and its reactions to the latest enlargement of NATO. This paper examines Türkiye’s evolving Transatlantic identity through the lens of ontological security, according to which states act in order to provide continuity in their identities (Türkiye’s Transatlantic identity) and to prevent any instabilities to feel ontologically secure, which then might create security dilemmas between the protective security cocoons (Türkiye’s NATO membership) and the crises with other actors (Türkiye’s clashes with/within NATO). In other words, this paper aims to understand how Türkiye’s Transatlantic identity is still valued, desired, and reconstructed, despite the security dilemmas of Türkiye’s contentions with/within the NATO Alliance, from the ontological security perspective.

Revealing the metabolic potential and environmental adaptation of nematophagous fungus, Purpureocillium lilacinum, derived from hadal sediment

The extreme environment shapes fungi in deep-sea sediments with novel metabolic capabilities. The ubiquity of fungi in deep-sea habitats supports their significant roles in these ecosystems. However, there is limited research on the metabolic activities and adaptive mechanisms of filamentous fungi in deep-sea ecosystems. In this study, we investigated the biological activities, including antibacterial, antitumor and nematicidal activity of Purpureocillium lilacinum FDZ8Y1, isolated from sediments of the Mariana Trench. A key feature of P. lilacinum FDZ8Y1 was its tolerance to high hydrostatic pressure (HHP), up to 110 MPa. We showed that HHP affected its vegetative growth, development, and production of secondary metabolites, indicating the potential for discovering novel natural products from hadal fungi. Whole-genome sequencing of P. lilacinum FDZ8Y1 revealed the metabolic potential of this piezotolerant fungus in carbon (carbohydrate metabolism), nitrogen (assimilatory nitrate reduction and protein degradation) and sulfur cycling processes (assimilatory sulfate reduction). Transcriptomic analysis under elevated HHP showed that P. lilacinum FDZ8Y1 may activate several metabolic pathways and stress proteins to cope with HHP, including fatty acid metabolism, the antioxidant defense system, the biosynthetic pathway for secondary metabolites, extracellular enzymes and membrane transporters. This study provides valuable insights into the metabolic potential and adaptation mechanisms of hadal fungi to the challenging conditions of the hadal environment.

Cold Tolerance and Physiological Response of Natural Overwintering Pomacea canaliculata in South China.

Pomacea canaliculata (Lamarck 1822), a freshwater gastropod indigenous to lower Del Plata Basin of Argentina, has become the most destructive and invasive rice pests in south China since its introduction in the 1980s. In Guangdong, the main production areas for double rice, most of P. canaliculata overwinter in paddy field ditches after late-rice harvesting in mid-November and diapause to temporarily to avoid the damaging effects of extreme low temperatures. This pest aroused from diapause and migrated to the paddy field after early-rice reviving in next late March. Overwintering and cold tolerance of natural P. canaliculata have a non-negligible impact on population dynamics and distribution in the following year. We tested the supercooling capability, levels of cryoprotectant synthesis, activity of antioxidant defense system (antioxidant enzymes and reduced glutathione), and degree of oxidative damage (concentration of malondialdehyde as an index of lipid peroxidation) monthly, using natural P. canaliculata samples with a size-gender structure (i.e., juveniles, female, and male adults) from experimental ponds during the period of mid-November to the following April. P. canaliculata survived the winter with a monthly death rate of 7%-16.5% in coldest January. The supercooling point (SCP) of overwintering P. canaliculata decreased initially before increasing subsequently with monthly changes in water temperature. P. canaliculata accumulated a high glycogen content before December, which depleted towards the end of January, while lipid content reached peak in January and depleted since February. Activity of antioxidant defense system of P. canaliculata exhibited significant monthly differences and showed relatively higher size heterogeneity than monthly variations. The results contribute to the knowledge of adaptability in overwintering P. canaliculata and help to understand the mechanism of the invasive success of this species.

A Network Defense System for Detecting and Preventing Hacking

Many computing systems have recently suffered significantly from hacking and preventing hacking is important in protecting business, sensitive information and every day network communications. Many efforts have been made to provide security assurance by proposing security solutions. However, the gap between hacking incidents and current security solutions is significant. Fortunately, hacking attempts can be addressed if the pre-hacking step, called scanning, is properly investigated and good counter measures are in place. The importance of scanning appears is in providing sophisticated hackers with the necessary information about nominated victims systems which eventually forms their hacking strategies. Therefore, this article proposes a security solution that aims to make scanning difficult by addressing its properties, which makes developing hacking strategies against protected computer networks unpractical. The proposed security solution protects computer networks by dynamically generating a unique protocol to replace the expected standard protocols and changing network paths periodically in order to confuse scanning attempts, as well as to prevent unauthorized scanning and hacking traffic. Key Words: hacking,sensitive information, unauthorized scanning, hacking traffic, network communications

Vanillic Acid Modulates Antioxidant Defense and Methylglyoxal Detoxification Systems to Combat Drought Stress in Tomato Seedlings

Vanillic acid (VA) regulates various plant physiological and biochemical processes upon different environmental stresses to enhance their tolerance. This study aimed to evaluate the protective effect of VA on growth and physiology, including osmoprotection, and antioxidant defense systems for enhancing higher tolerance by lowering oxidative damage against water deficit stress in tomatoes (Solanum lycopersicum L. cv. BARI Tomato-16). Hydroponically grown tomato seedlings (8 d old) were pretreated with 50 µM VA for 2 days followed by water deficit stress (imposed by water withdrawal and 12% polyethylene glycol; PEG-6000) for 4 d. Drought stress inhibited the seedlings’ growth by reducing water content and photosynthetic pigments contents, alleviating oxidative stress induced by a reactive oxygen species and methylglyoxal. A significant enhancement in growth, biomass accumulation, and photosynthetic pigment content was observed in VA-pretreated stress conditions. In addition, there was an improvement in the water status and proline content, along with modulated activities of the antioxidant responses, including both non-enzymatic and enzymatic components in leaves of VA-pretreated seedlings upon the water deficit. Vanillic acid significantly reduced the reactive oxygen species generation and decreased cellular membrane damage in drought-affected tomato seedlings. Methylglyoxal detoxification was ensured to a great extent in VA-pretreated stressed tomato seedlings by strengthening the glyoxalase enzymes’ activities. Therefore, VA can be effective for protecting tomato seedlings by inducing a plant antioxidant defense and the methylglyoxal detoxification system and osmoregulation under drought stress.

New Horizons in Micro/Nanoplastic-Induced Oxidative Stress: Overlooked Free Radical Contributions and Microbial Metabolic Dysregulations in Anaerobic Digestion.

Excessive production of reactive oxygen species (ROS) induced by micro/nanoplastics (MPs/NPs) is highly toxic to microbes. However, the mechanisms underlying ROS generation and metabolic regulation within anaerobic guilds remain poorly understood. In this study, we investigated the effects of environmentally relevant levels of polypropylene (PP)-MPs/NPs on oxidative stress and microbial ecology during anaerobic digestion (AD). Electron paramagnetic resonance spectroscopy revealed that PP-MPs/NPs elevated the concentrations of environmentally persistent free radicals (EPFRs) and derived hydroxyl radicals (•OH). EPFRs were identified as the primary contributors to •OH generation, as evidenced by a high Spearman correlation coefficient (r = 0.884, p < 0.001) and free radical-quenching studies. The formation of •OH enhanced ROS production by 86.2-100.9%, resulting in decreased cellular viability and methane production (by 37.5-50.5%) at 100 mg/g TS PP-MPs/NPs. Genome-centric metagenomic and metatranscriptomic analyses suggested that PP-MPs/NPs induced the reassembly of community structures, re-evolution of functional traits, and remodeling of interspecies interactions. Specifically, PP-MPs/NPs induced a shift in methanogen consortia from hydrogenotrophic Methanofollis sp. to acetoclastic and hydrogenotrophic Methanothrix soehngenii, primarily because of the latter's diverse ingestion patterns, electron bifurcation complexes, and ROS-scavenging abilities. Downregulation of genes associated with antioxidative defense systems (i.e., sodN, katA, and osmC) and ROS-driven redox signal transduction pathways (c-di-AMP and phosphorylation signaling pathways) provided insights into the mechanisms underlying ROS-induced microbial metabolic dysregulation. Our findings enhance the understanding of microbial ecological and metabolic traits under MPs/NPs stressors, facilitating the control of MPs/NPs toxicity and the stabilization of AD processes.

Japan Joins Aukus: Motivations, Progress, And Impact

Since its establishment, the AUKUS defense system has encountered significant developmental challenges, primarily due to insufficient internal driving forces, which has created a strategic opportunity for Japan to join AUKUS. Japan seeks to enhance its own defense capabilities, deepen the Japan-U.S. alliance, and define its role in the Indo-Pacific strategy, while also aiming to form close ties with allies in high-tech fields. The U.S. and UK support Japan’s entry into AUKUS and expect to expand the alliance through strategic interaction and technological alliances. However, Australia, considering factors such as geopolitical strategy, institutional construction, and domestic politics, has shown a cautious attitude. Japan’s joining of AUKUS will impact the security structure of the Indo-Pacific region, potentially intensify the regional arms race, and jeopardize the surrounding environment for China’s development.

The effects of SOD Melon Gliadin as an Antioxidant on VEGF and TGF β blood serum and vitreoretina in hyperglycemia rat model a literature review

Diabetes Mellitus is a chronic metabolic disorder characterized by hyperglycemia resulting from impaired insulin secretion, action, or both. Persistent hyperglycemia can lead to organ damage, including ocular complications such as diabetic retinopathy. Diabetic retinopathy is a primary cause of preventable blindness and can become vision-threatening if complications are persistent. Over one-third of individuals with diabetes exhibit signs of diabetic retinopathy, and among them, approximately one-third experience vision-threatening diabetic retinopathy (VTDR), defined as severe non-proliferative diabetic retinopathy (NPDR), proliferative diabetic retinopathy (PDR), or the presence of diabetic macular edema (DME). Diabetic retinopathy triggers the production of reactive oxygen species (ROS). Excessive accumulation of ROS can damage tissues surrounding retinal blood vessels, leading to oxidative stress that compromises the blood-retina barrier (BRB). Vascular endothelial growth factor (VEGF) and transforming growth factor-beta (TGF-β) are two markers that indicate retinal dysfunction. Cells possess an intrinsic defense system against ROS, comprising antioxidants such as superoxide dismutase (SOD), glutathione reductase, glutathione peroxidase, and catalase. SOD melon gliadin (Glisodin®) is a potent antioxidant that elevates endogenous SOD levels, reduces ROS, and protects retinal tissues from damage and cell death. Research on SOD melon gliadin (Glisodin®) shows its potential as an antioxidant for preventing diabetic complications in the retina, specifically diabetic retinopathy.

Carbon dots based cascading nanozymes mitigate phytotoxicity in lettuces under imidacloprid stress

Improper pesticide use induces oxidative stress and disrupts detoxification systems in plants. We synthesized CDs with cascading nanozyme activities to mitigate phytotoxicity in lettuces under imidacloprid (IMI) stress. CDs exhibit superoxide dismutase-like (SOD-like) and peroxidase-like (POD-like) activities. Surface modifications and analysis of CDs, the SOD-like activity relies on the -NH2, -COOH, and -OH groups for binding superoxide anions (O2•-), while POD-like activity depends on -COOH and CO groups, also, CO group provides π-system and the electron-deficient structure for electron transfer. Practically, under IMI stress, CDs strengthen multiple defense systems in lettuces, reducing levels of reactive oxygen toxicity (O2•-, H2O2, and MDA, by 26.77 %, 48.52 %, and 13.10 %, respectively). Meanwhile, CDs upregulate detoxification gene expression, resulting in a 42.74 % reduction in IMI residue in lettuces. Moreover, the acceptable daily intake of IMI in lettuces treated with CDs was less than 18.0 % of the reference dose, even at high-concentration IMI.

Phylogenetic evaluation and genotypic identification of burn-related Pseudomonas aeruginosa strains isolated from post-burn human infections during hospitalization.

Cutaneous burn trauma, compromise of dermal layers and immune defense system is a physical and fiscal burden on healthcare systems. Burn-wound infections are a serious complication of thermal-injury and contribute significantly to care burden. After burn-induced trauma, sepsis by Pseudomonas aeruginosa impairs patient recovery and contributes to mortality and morbidity. Past studies show positive correlation between detection of Pseudomonas species and healing-impaired traumatic wounds. P. aeruginosa is a resilient opportunistic human pathogen and a nosocomial agent involved in pathology of healing-impaired wounds, especially in burn-patients. Expansive array of virulence determinants have resulted in gentamicin- and silver-resistant P. aeruginosa outbreaks. Knowledge of molecular dynamics and phylogeny of P. aeruginosa associated with burn-wounds is limited. Therefore, we conducted whole-genome sequencing for genotyping and phylogenetic analysis of P. aeruginosa burn-associated strains (n=19) isolated from 7 burn cases during hospitalization. Comparison of genetic features in P. aeruginosa strains in the core genome and mobilome detected genetic variations within some clonal infections overtime. Genetic variations were observed among different burn cases, with some features identified in severe lung infections. Polyclonal infections were also observed, with differing genotypes and virulence potentials, highlighting the importance of reasoned sampling of isolates for clinical testing.

Incretin-Based Therapies: A Promising Approach for Modulating Oxidative Stress and Insulin Resistance in Sarcopenia.

Recent studies have linked sarcopenia development to the hallmarks of diabetes, oxidative stress, and insulin resistance. The anti-oxidant and insulin sensitivityenhancing effects of incretin-based therapies may provide a promising option for the treatment of sarcopenia. This review aimed to unveil the role of oxidative stress and insulin resistance in the pathogenesis of sarcopenia and explore the potential benefits of incretin-based therapies in individuals with sarcopenia. PubMed, the Cochrane Library, and Google Scholar databases were searched by applying keywords relevant to the main topic, to identify articles that met our selection criteria. Incretin-based therapies manifested anti-oxidant effects by increasing the anti-oxidant defense system and decreasing free radical generation or by indirectly minimizing glucotoxicity, which was mainly achieved by improving insulin signaling and glucose homeostasis. Likewise, these drugs exhibit insulin-sensitizing activities by increasing insulin secretion, transduction, and β-cell function or by reducing inflammation and lipotoxicity. Incretin-based therapies, as modulators of oxidation and insulin resistance, may target the main pathophysiological factors of sarcopenia, thus providing a promising strategy for the treatment of this disease.

Optimalisasi Proses Bisnis Menggunakan Metode Business Process Analysis (BPA) pada PT. XYZ

PT XYZ is one of the strategic industries owned by BUMN that produces the main defense equipment for Indonesia's defense system, especially in the maritime sector. In practice, the process of utilizing and managing assets at PT XYZ is in the Asset &amp; Infrastructure Optimization Department which has various operational steps for asset management starting from the asset mapping process to utilizing these assets. This research aims to analyze and optimize business processes in asset management in the Asset &amp; Infrastructure Optimization Department. The research method used is observation and interviews with related parties as well as Business Process Modeling and Notation analysis. The purpose and objective is to provide recommendations in optimizing business processes and increasing efficiency in managing company assets. One of the problems identified was the slow signing and return of agreement letters which caused delays in issuing invoices and payments. Recommendations included creating standard operating procedures, implementing electronic-based contracts and invoices, and utilizing technology for document delivery and storage. Overall, this research aims to improve operational performance and maximize the contribution of PT XYZ's assets to revenue.

Gambaran Jumlah Leukosit pada Penderita Demam Berdarah Dengue di Klinik DR.Trisna Garut

Dengue hemorrhagic fever (DHF) is a public health problem in Indonesia whose number of cases continues to increase and its spread is increasingly widespread.Tests used to diagnose dengue fever include a white blood cell count test.White blood cells are cells that play a role in the body's defense system. The reason is, white blood cells help fight infections in the body, such as viruses, bacteria, parasites and fungi. In patients infected with dengue fever, the white blood cell count is usually normal but then decreases (leukopenia) and neutropenia becomes dominant. Thisresearch was conducted from March to April 2023.The aim of this study was to measure the number of white blood cells in dengue fever patients.This type of research is descriptive. The research was carried out using an automatic analysis method (blood analysis) in the laboratory ofKlinik Dr.Trisna Garut, using samples from 30 dengue fever patients who were tested. In this study, the number of white blood cells in dengue fever patients at Klinik Dr.Trisna Garut was 14 (46.7%).Wich is Normal And the white blood cell count results of dengue fever patients at Klinik Dr.Tisna Garut General Hospital decreased by 16 (54.3%).

FOREWORD AND FAREWELL

As I write my final foreword as Editor-in-Chief of the Journal of Naval Sciences and Engineering (JNSE), it is with deep gratitude and pride that I look back on the transformative progress we have achieved together. I first took on this role with the responsibility of publishing the second issue of 2022, just as the journal transitioned from the Naval Sciences and Engineering Institute to the Turkish Naval Academy (TNA). Now, following my promotion to Rear Admiral (Lower Half) and my appointment as the Deputy General Manager of Naval Shipyards, I prepare to step down, filled with confidence in the journal's future impact. During my tenure, JNSE has not only expanded its reach but also evolved in structure. As the first Editor-in-Chief after this significant transition, I established a structured workflow to ensure the smooth handling of the journal’s workload. This progress would not have been possible without the unwavering support of our esteemed contributors, editors, reviewers, and readers, who have collectively strengthened the journal’s place as a respected publication in naval sciences and engineering. Naval sciences and engineering have always been at the forefront of the Turkish Navy’s priorities, with an increasing emphasis on innovation and self-reliance in defense capabilities. As researchers and engineers, our collective work—through publications like this journal—contributes, even if indirectly, to these larger goals. It is essential to recognize that advancements in naval platforms, weaponry, and defense systems depend heavily on foundational research in science and technology. Every study, every paper, and every breakthrough—however modest it may seem—plays a role in building the knowledge base that supports these ambitious naval goals. In this foreword, I highlight recent and ongoing projects of the Turkish Navy, from initial concepts to construction, to offer future contributors an idea of impactful areas of study. Research in fields like autonomous systems, AI-driven operations, and advanced propulsion technologies can shape the Navy’s future capabilities. As the quote often attributed to Faraday or Franklin reminds us, "What is the use of a newborn baby?" Theoretical research, like this newborn, may not reveal its full utility immediately, yet it holds the potential to make profound impacts over time. (Full foreword available in the PDF version.) As I transition from this role, I am proud of the journal’s achievements. One of the most notable milestones has been the introduction of Digital Object Identifiers (DOIs) for all published papers since the second issue of 2022. This change has allowed for greater visibility, wider dissemination, and immediate online publication, which are crucial in the fast-paced world of research. Additionally, reorganizing the editorial team, including the appointment of key roles such as the Technical Editor, Layout Editor, and Secretariat from the capable staff of the TNA has ensured a streamlined workflow and elevated the journal's standards. The December 2024 issue is a testament to the journal’s interdisciplinary reach. This issue includes four valuable studies: one presenting a robust planning model for irrigation, a topic of relevance as climate change increasingly impacts maritime and agricultural resources; a second, developing a cybersecurity perception scale for maritime industry employees, reflecting the growing importance of cybersecurity in naval operations; a third, a performance analysis of adaptive MIMO techniques for visible light communication, which holds promise for improving naval communication systems; and finally, a paper on thyroid nodule segmentation using ultrasound, which highlights how advances in medical imaging can also improve healthcare aboard naval vessels. These papers, though diverse, reflect the journal's goal of fostering interdisciplinary innovation. As I conclude my tenure as Editor-in-Chief, I want to express my gratitude one last time to the readers, the dedicated editors who served alongside me, and the referees who ensured the quality of the research we published. It has been a privilege to oversee the journal through significant milestones, and I deeply appreciate the effort and commitment of everyone involved. Reflecting on my tenure, I am particularly proud of the journal's growth, both in scope and influence. Over these years, we have not only expanded our reach but have also contributed to shaping the dialogue around the future of naval technology. Though this marks the end of my tenure, I depart with the satisfaction of knowing that we have upheld the highest standards in naval science and engineering research. As I bid farewell, I leave with the hope that the journal continues to inspire and contribute to the advancement of naval sciences. May your journey be swift and your destination secure. Fatih ERDEN, Ph.D. Editor-in-Chief

Space cybersecurity challenges, mitigation techniques, anticipated readiness, and future directions

Space Cybersecurity (SC) is becoming critical due to the essential role of space in global critical infrastructure – enabling communication, safe air travel, maritime trade, weather monitoring, environmental surveillance, financial services, and defence systems. Simultaneously, involving diverse stakeholders in space operations further amplifies this criticality. Similarly, previous research has identified isolated vulnerabilities in SC and proposed individual solutions to mitigate them. While such studies have provided useful insights, they do not offer a comprehensive analysis of space cyber-attack vectors and a critical evaluation of the effectiveness of mitigation strategies. This study addresses this problem by holistically examining the scope of potential space cyber-attack vectors, encompassing the ground, space, user, cloud, communication channels, and supply chain segments. Furthermore, the study evaluates the effectiveness of legacy security controls and frameworks and outlines SC-vector-aligned counterstrategies and mitigation techniques to tackle the unique SC threats. Based on the analysis, the study proposes future research directions to develop and test advanced technological solutions and regulatory and operational frameworks to establish international standards policies and foster stakeholder collaboration. The study contributes a multi-disciplinary foundation and roadmap that researchers, technology developers, and decision-makers can draw on in shaping a robust and sustainable SC framework.

Do Different Wheat Ploidy Levels Respond Differently Against Stripe Rust Infection: Interplay between Reactive Oxygen Species (ROS) and the Antioxidant Defense System?

Wheat stripe rust (Puccinia striiformis f. sp. tritici, Pst) is the most damaging wheat disease, causing substantial losses in global wheat production and productivity. Our study aimed to unravel the complex reciprocity between reactive oxygen species and the antioxidant defense system as a source of resistance against stripe rust in diploid, tetraploid and hexaploid wheat genotypes. The significant genetic variability for stripe rust in the materials under study was evident as the genotypes showed contrasting responses during both the adult and seedling stages. Our thorough perspective on the biochemical responses of wheat genotypes to stripe rust infection revealed distinct patterns in oxidative damage, antioxidant enzymes and photosynthetic pigments. Principal component analysis revealed inverse correlations between antioxidants and ROS, underscoring their key function in maintaining the cellular redox balance and protecting plants against oxidative damage. Diploid (Ae. tauschii) wild wheat exhibited a better biochemical defense system and greater resistance to stripe rust than the tetraploid (T. durum) and hexaploid (Triticum aestivum) wheat genotypes. The antioxidant enzyme activity of durum wheat was moderate compared to diploid and hexaploid wheat genotypes. The hexaploid wheat genotypes exhibited increased ROS production, reduced antioxidant enzyme activity and decreased photosynthetic pigment levels. This study enhances understanding of the antioxidant defense system across different wheat ploidies facing stripe rust, serving as a valuable strategy for improving crop disease resistance. This study validated the biochemical response of stripe rust-resistant and susceptible candidate genotypes, which will be used to develop genetic resources for discovering stripe rust resistance genes in wheat.

Plasma processed Zn fortification: the next generation sustainable technology for the improvement of agronomic traits of paddy

Zinc performs different metabolic functions in human body and its deficiency is a major concern mainly in the third world countries. Fortification of Zn in grain crops may have the possibility to meet the challenge. The present study was designed utilizing plasma technology for paddy crop in two ways: (i) seed treatment with gliding arc argon-oxygen-ZnSO4-H2O discharge plasma jet in combination with nebulizing system for the fortification of Zn in the seeds, and (ii) plasma treated water (PTW) was sprayed to the plants cultivated from treated seeds and thereby investigated the growth and development of paddy plants defence system, nutritional composition and yield of paddy. Gliding arc argon-oxygen-ZnSO4-H2O discharge plasma jet was employed to treat paddy seeds with nebulizing system for the duration of 2-, 4-, 6-, and 8 min and the 4 min treatment condition provided the maximum 98% germination among the conditions considered. Seedlings germinated from the 4 min treated seeds were resettled in the research area and PTW were applied two, four, and six times at the time of their growing period. The results reveal that the growth parameters, and the plants defence mechanisms were enhanced. Further, Zn concentration in the grains and the yield of paddy were increased by 59.07%, 24.06% and 38.94%, 22.27% respectively, compared to control and the plants grown from the treated seeds where no PTW foliar spray was applied. This investigation exposed that the fortification of Zn in seed by plasma processing and PTW spray to the plants which in turn improved seed germination, enzymatic activities, Zn accumulation in rice, and yield. It is fascinating that the paddy harvesting time was reduced more than two weeks compared to control.

Hepatic enzyme induction and its potential effect on thyroid hormone metabolism in the metamorphosing tadpole of Xenopus laevis (African clawed frog).

Hepatic enzyme induction, an inherent defense system against xenobiotics, is known to simultaneously affect endocrine system functions in mammals under specific conditions, particularly thyroid hormone (TH) regulation. While this phenomenon has been studied extensively, the pathway leading to this indirect thyroid effect in mammals has unclear applicability to amphibians, despite the importance of amphibian species in assessing thyroid-disruptive chemicals. Here, we investigated the effects of three well-known mammalian enzyme inducers-β-naphthoflavone (BNF), pregnenolone carbonitrile (PCN), and sodium phenobarbital (NaPB)-on the gene expression of phase-I and phase-II metabolizing enzymes in Xenopus laevis tadpoles. Waterborne exposure to BNF and PCN significantly induced the expression of both phase-I (cytochrome P450, CYP) and phase-II enzymes (UDP-glucuronosyltransferase, UGT and sulfotransferase, SULT), but in different patterns, while NaPB exposure induced CYP2B expression without affecting phase-II enzymes in tadpoles, in contrast to mammals. Furthermore, an ex vivo hepatic enzyme activity assay confirmed that BNF treatment significantly increased phase-II metabolic activity (glucuronidation and sulfation) toward TH. These results suggest the potential for certain mammalian enzyme inducers to influence TH clearance in X. laevis tadpoles. Our findings provide insights into the profiles of xenosensing activity and enzyme induction in amphibians, which can facilitate a better understanding of the mechanisms of indirect effects on the thyroid system via hepatic enzyme induction in nonmammalian species.

Investigating the hepatoprotective and antidiabetic properties of cryogenically pulverized Turkish propolis water extracts in streptozotocin-induced diabetic rats

Diabetes is a major global health crisis, affecting millions of people worldwide and necessitating the search for effective and safe treatments, leading to various complications, including liver damage. This study investigated the hepatoprotective and antidiabetic properties of cryogenically pulverized Turkish propolis water extracts (WBTP) in streptozotocin-induced diabetic rats.Propolis samples were collected from the West Black Sea Region of Turkey and prepared as -80 °C (PP-80) and -196 °C (PP-196) water extracts. Thirty male Wistar albino rats were divided into five groups: normal control, diabetic control, diabetic + PP-80 (250 mg/kg), diabetic + PP-196 (250 mg/kg), and diabetic + metformin (250 mg/kg). After 35 days of treatment, various parameters were evaluated, including body weight, blood glucose, glycated hemoglobin (HbA1c), serum biochemistry, antioxidant enzyme activities, and histopathology of the pancreas.The results showed that WBTP extracts, especially PP-80, significantly improved body weight and reduced blood glucose and HbA1c levels. Analysis of lipid profiles revealed improvements in HDL, LDL, triglycerides, and total cholesterol levels with WBTP treatment. The antioxidant defense systems were also enhanced in the WBTP-treated groups. Histopathological examination revealed that WBTP extracts mitigated pancreatic tissue damage in the diabetic rats.In conclusion, the WBTP extracts possess hepatoprotective and antidiabetic properties, suggesting a promising avenue for further research. It emphasizes the importance of addressing both diabetes and hepatopathologies concurrently and highlights WBTP extracts as natural and potentially safer alternatives to synthetic antidiabetic drugs. Specifically, PP-80 extracts demonstrate robust effects in managing diabetes and associated liver complications in streptozotocin-induced diabetic rats, indicating their therapeutic potential.