This study explores the sugarcane bagasse (SCB) pretreatment with two deep eutectic solvents (DESs) prepared from choline chloride (ChCl) as a hydrogen bond acceptor (HBA) to two hydrogen bond donors (HBD): oxalic acid (OA) and trifluoroacetic acid (TFA) for lignin recovery and enhancing enzymatic saccharification. The pretreatment conditions, reaction time, temperature, and DES concentration were optimized using response surface methodology. The two DESs showed a lignin yield of 74.67 ± 2.75 and 83.50 ± 2.65 mg per gm of SCB, respectively. Enzymatic hydrolysis of the cellulose-enriched material (CEM) obtained after pretreatment showed an enhanced RS yield of around 4-fold compared to the untreated SCB. The CEM was characterized using FTIR, Powder X-ray diffraction, and Scanning Electron Microscopy. The recovered lignin characteristics were studied using FTIR, 1H NMR, and 2D-HSQC NMR analysis. The FTIR spectra of lignin show the presence of aromatic skeletal vibrations, -OH, -CH, C-O stretching vibration, and aromatic C-H deformation. Furthermore, the pretreatment solvent recyclability studies were conducted to evaluate the effectiveness of DES for a sustainable biorefinery process.