Bamboo is a naturally occurring composite material, which exhibits a decomposable structure with varying composition. The distinct structural features of bamboo contribute to its exceptional strength and flexibility, making it an excellent choice for construction purposes. However, only a limited portion of bamboo species has been studied for its mechanical properties, and research on Bambusa arundinaceae has primarily focused on its pharmaceutical values. Therefore, we investigated the relationship between the structural characteristics of B. arundinaceae and its mechanical properties using axial compression experiments and tangential bending experiments. The results showed that the distribution density of vascular bundles (VBs) of B. arundinaceae ranged from 1.98 to 4.34 pcs/mm2,while the volume fraction of fiber sheaths (FSs) ranged from 35.82 to 42.58%. The average compressive strength, flexural strength, and flexural elasticity modulus were 113.99 MPa, 239.07 MPa, and 17.39 GPa, which were 97.56%, 64.07%, and 66.09% higher than those of moso bamboo (Phyllostachys edulis), respectively. The compressive strength, flexural strengths, and elasticity modulus of B. arundinaceae were positively correlated with both the distribution density of VBs and the volume fraction of FSs. These insights are crucial for the advancement of durable and efficient materials in diverse sectors including construction and manufacturing.