Decenoic Acid Research Articles

Characterization of the Cytotoxic Compounds of Lactarius salmonicolor R. Heim and Leclair by Gas Chromatography-Mass Spectrometry and Chemometrics

The cytotoxic activities of apolar extract of Lactarius salmonicolor and its fractions and subfractions were screened against colon (CaCo-2), prostate (LNCaP), lung (H1299), and breast (MCF-7) cancerous cell lines. The main extract of Lactarius salmonicolor that was cytotoxic to CaCo-2 (EC50: 137.1 ± 2.7 µg/mL) and LNCap (EC50: 131.2 ± 2.6 µg/mL) mainly contains fatty acids (99.9%). To characterize the cytotoxic activity, it was fractionated by a silica gel column. Two cytotoxic fractions were re-fractionated to obtain subfractions. The extract, four cytotoxic fractions, and all subfractions were analyzed by gas chromatography-mass spectrometry (GC-MS). The GC-MS data and the cytotoxic activity (EC50) results were classified using principal component analyses (PCA). According to PCA, the cytotoxic fractions containing abundant benzoic acid (0.01%–0.35%), cinnamic acid (0.01%–0.36%), azelaic acid (0.01%–0.92%), 2,4-decadienal (0.01%–0.12%), (Z)-9-palmitoleic acid (0.01%–1.09%), 18-hydroxy 2,4-diene oleic acid (0.01%–26.71%), arachidic acid (0.01%–6.70%), desmosterol (0.01%–12.27%), and steroids (0.01%–9.51%) were clustered. LSP.9-10, which causes another cluster, is caused by 8,11,14- eicosatrienoic acid, 11,14,17-eicosatrienoic acid, 9(Z)-decenoic acid compounds, and saturated fatty acids with 10 and 16 carbons. Hence, these compounds may be responsible for the cytotoxic activity. This study demonstrates that GC-MS and chemometric analysis can identify the components responsible for biological activity in combination with biological activity data.

Физико-химические показатели гомогената трутневого расплода, адсорбированного на безлактозных адсорбентах в процессе хранения

Abstract. Drone brood is a product that exhibits high biological activity, so its use can be recommended to a wide range of people as an adaptogenic, actoprotective and antioxidant agent. The high concentration of biologically active substances, such as decenoic acids, unsaturated fatty acids, sulfhydryl groups, sugars, hormones and hormone-like compounds makes drone brood a product very sensitive to environmental factors, which necessitates its stabilization. To stabilize drone brood homogenate, in a number of countries, a lactose-glucose adsorbent is usually used. The relevance of the work is due to the widespread prevalence of lactase deficiency among the population, the prevalence of which among the Eastern Slavs reaches 16–18 %, and among some peoples of Asia, Australia and America exceeds 80 %, which limits the use of adsorbed drone brood using a classical adsorbent containing lactose. The purpose of the work is to develop an innovative product based on drone brood that does not contain lactose as an adsorbent, and to trace the dynamics of the preservation of its biologically active components during storage. Potato starch was used as a lactose substitute in the adsorbent. This product can be recommended to a wider range of consumers. The work used methods for determining the physicochemical parameters of products, such as humidity, oxidation index, hydrogen index, mass fraction of decenoic acids, mass fraction of crude protein, free acidity, acid number, iodine number. The scientific novelty lies in the development of formulations for new products based on drone brood that do not contain lactose and the determination of the optimal product among them in terms of technological and physicochemical parameters. As a result of the studies, it was found that an adsorbent consisting of 80 parts of starch and 20 parts of glucose provides optimal product performance at a ratio of adsorbent to drone brood homogenate of 3 : 1.

A Hitchhiker’s Ride: The Honey Bee Louse Braula Coeca (Diptera: Braulidae) Selects its Host by Eavesdropping

The bee louse Braula spp. had until recently a distribution coincident with its host the honey bee. The adult fly usually attaches to a worker honey bee and steals food from its mouth. However, not all worker bees carry Braula spp. and the mechanism used by Braula spp. to select hosts is not well understood. Using choice remounting bioassays and chemical analyses, we determined host selection and the cues used by B. coeca, a species associated with the African honey bee Apis mellifera scutellata. Braula coeca successfully remounted bees from which they were initially removed and preferred their mandibular gland pheromones (MDG) over those of bees not carrying them. The bee lice did not show any preference for the cuticular hydrocarbons of both types of workers. Chemical analyses of the MDG extracts, revealed quantitative differences between the two categories of workers, with workers carrying B. coeca having more of the queen substance (9-oxo-2(E)-decenoic acid) and worker substance (10-hydroxy-2(E)-decenoic). Braula coeca showed a dose response to the queen substance, indicating its ability to use host derived kairomones as cues that allowed it to benefit from trophallactic dominance by individuals that have a higher probability of being fed by other workers.

Optimization of ultrasound-assisted extraction of fatty acids from royal jelly and its effect on the structural and antioxidant property

Fatty acids are the key active components in royal jelly (RJ) with various biological activities. In this study, a novel ultrasound-assisted extraction (UAE) method was established to extract fatty acids from RJ and their structural and antioxidant property were further evaluated. The optimum extraction conditions were as follows: liquid-to-solid ratio of 10:1, ultrasonic power of 450 W and ultrasonic duration of 20 min, resulting in a better extraction yield of 16.48 % and 10-hydroxy-2(E)-decenoic acid (10-HDA) content of 4.12 %. Furthermore, compared with the solvent extraction method, the antioxidant activity of extract by ultrasound was enhanced significantly by at least 448 %. GC–MS showed that ultrasound didn’t change the chemical composition of fatty acids, while it significantly increased the content of fatty acids. SEM image illustrated that extracts by UAE showed a rougher, looser microstructure compared to the solvent method. Overall, UAE is a promising method to obtain fatty acids in RJ with high efficiency.

Biologically-Relevant Staphylococcus Aureus Biofilm Phenotype Characterisation And Liability To Novel Antibiofilm Drugs.

To characterise the biofilm matrix composition of a newly described Staphylococcus aureus biofilm phenotype. This experimental study was conducted at the Faculty of Pharmacy, Helwan University, Cairo, Egypt, from January 2021 to March 2022, and comprised methicillin-resistant Staphylococcus aureus and methicillin-susceptible Staphylococcus aureus biofilm-forming clinical isolates which were allowed to construct biofilms under two distinct culture conditions; one a commonly used condition, and the other one a novel, more biologically-relevant condition. The formed biofilms were analysed for matrix composition through treatment with proteinase,sodium meta-periodate, and streptokinase. The efficacy of Cis-2-Decenoic acid and hamamelitannin on the biologically-relevant biofilms was evaluated using biofilm viability assay based on a colorimetric assay for measuring cell metabolic activity and scanning electron microscope imaging. Data was analysed using GraphPad Prism 5.01. Of the 58 isolates, 45(77.6%) were methicillin-resistant Staphylococcus aureus and 13(22.4%) were methicillin susceptible Staphylococcus aureus. There was significant difference in responses to streptokinase, proteinase and sodium meta-periodate (p<0.05) among the differentially-developed biofilms in methicillin-resistant Staphylococcus aureus isolates. Regarding the methicillin-susceptible Staphylococcus aureus isolates, the differentially-developed biofilms showed significantly different liabilities to streptokinase only (p<0.05). Mean biofilm inhibition for Cis-2- Decenoic acid was 54.27±27.93% and mean biofilm dispersion was 71.92±11.59% while the corresponding valuesfor hamamelitannin were 83.03±13.95% and 70.48±7.116% against the newly described methicillin-resistant Staphylococcus aureus biofilm phenotype. Applying biologically-relevant culture conditions on staphylococci biofilms and antibiofilm drugs is recommended.

Changes in the chemical composition of royal jelly produced through artificial bee-feeding in response to seasonal variations during non-migratory beekeeping

A reliable database was established from the analysis of 60 royal jellies (RJs) produced by bee colonies selectively provided with artificial feed according to seasonal changes at an apiary in Gyeongsangnam-do, Korea. The moisture content of RJs ranged from 60.1 % to 67.1 % (average 65.2 %), and the trans-10-hydroxy‐2–decenoic acid (10-HDA) content ranged from 1.4% to 2.5% (average 1.9 %), indicating a wide range during the harvest period. The δ13C values of the RJs varied from − 24.0‰ to − 17.2‰ (average −19.3‰). Sugar contents were 1.9–6.1 % for fructose, 2.8–5.5 % for glucose, and 0.7–7.7 % for sucrose. Pearson correlations were determined between harvest time and RJ components. The 10-HDA content showed a weak negative correlation with harvest time (p < 0.01). The δ13C values and sucrose contents showed a weak positive correlation with harvest time when artificial feed was selectively supplied to bees according to seasonal changes (p < 0.01). This study highlights the characteristics and composition ranges of Korean RJ and compares the differences between the tested samples and RJ samples produced outside Korea. The results provide information necessary for determining the authenticity and quality grade of RJ by investigating the effect of bee colonies selectively provided with artificial feed according to seasonal changes on the compositional changes of RJ.

Synergistic anti-proliferative activities of 10-hydroxy-2- decenoic acid in adjunct to doxorubicin in MCF-7 breast cancer cells

Synergistic anti-proliferative activities of 10-hydroxy-2- decenoic acid in adjunct to doxorubicin in MCF-7 breast cancer cells

Production of 10-Hydroxy-2-decenoic Acid from Decanoic Acid via Whole-Cell Catalysis in Engineered Escherichia coli.

10-Hydroxy-2-decenoic acid (10-HDA) is a terminal hydroxylated medium-chain α,β-unsaturated carboxylic acid that performs various unique physiological activities and has a wide market value. Therefore, development of an environmentally friendly, safe, and high-efficiency route to synthesize 10-HDA is required. Here, the β-oxidation pathway of Escherichia coli was modified and a P450 terminal hydroxylase (CYP153A33-CPRBM3 ) was rationally designed to synthesize 10-HDA using decanoic acid as a substrate via two-step whole-cell catalysis. Different homologues of FadDs, FadEs, and YdiIs were analyzed in the first step of the conversion of decanoic acid to trans- -2- decenoic acid. In the second step, CYP153A33 (M228L)-CPRBM3 efficiently catalyzed the conversion of trans- -2- decenoic acid to 10-HDA. Finally, 217 mg L-1 10-HDA was obtained with 500 mg L-1 decanoic acid. This study provides a strategy for biosynthesis of 10-HDA and other α, β-unsaturated carboxylic acid derivatives from specific fatty acids.

Influence of technology on the quality indicators of the composition of drone brood and royal jelly

Drone brood homogenate and compositions based on it are gaining popularity as functional food products and biologically active food additives. It has antioxidant, androgenic, anabolic, sedative, antidepressant, immunotropic, cosmetic and other properties. Royal jelly and drone brood have a pronounced biological activity, as they contain a large amount of decenoic acids, sulfhydryl groups, proteins, peptides and other biologically active substances. The possibility of expanding the spectrum of biological activity determines the relevance of developing a product containing a composition of drone brood and royal jelly. A significant superiority of the safety of biologically active substances in adsorbed drone brood and royal jelly in comparison with lyophilized ones was shown, which indicates the preferred method of stabilizing the combined product. The purpose of the work was to compare two technological methods for preparing a combined product. The first method consisted in the joint adsorption of royal jelly and drone brood, and the second - in separate adsorption and drying, followed by joint dry granulation of the components. It is shown that the differences in the physicochemical characteristics of products obtained by different methods are insignificant. The composition has intermediate values of physicochemical indicators between drone brood and royal jelly.

ЖИРНОКИСЛОТНЫЙ СОСТАВ МОЛОЧНЫХ ПРОДУКТОВ ПРИ ИХ МОДИФИКАЦИИ (ФАЛЬСИФИКАЦИИ)

The article presents research on the fat of chicken, goose, lamb, beef, sour cream and a milk-containing product with a milk fat substitute, produced using sour cream technology. There is a tendency towards the absence in the composition of a milk-containing product with a milk fat substitute, butyric, nylon, capric, caprylic, decenoic acids. Fatty acids are also absent in chicken, lamb, beef and goose fats, which makes it possible to exclude the use of these fatty acids in the production of a milk-containing product.

Application of drone brood in rational nutrition and apitherapy

Drone brood is a collection of developing individuals (larvae, prepupae and pupae) of drones - males of a bee colony. Drone brood is used to produce a homogenate, which is stabilized in one way or another to achieve optimal preservation of biologically active substances. Interest in drone brood as a rich source of macro- and micronutrients is steadily increasing. The purpose of this review is to summarize the collected data on the use of drone brood in rational nutrition and apitherapy. The indicators of the quality and safety of drone brood are reviewed, the requirements of regulatory documents for products based on it are given. The necessity of strict adherence to the storage conditions of the drone brood homogenate is shown, in case of violation of which there is a rapid deterioration of the homogenate. This necessitates stabilization of the drone brood, which makes it possible to preserve its properties with lower requirements for storage conditions. The groups of nutrients that make up the drone brood and determine its biological activity and directions of application are given. Among them are lipids, including phospholipids and unique decenoic acids, proteins, peptides and amino acids, substances containing sulfhydryl groups, flavonoid and other phenolic compounds, carbohydrates, steroid substances, including hormones and hormone-like compounds, mineral elements. Products based on drone brood and biological effects of their use are described: actoprotective, anabolic, immunomodulatory, endocrinotropic, neurotropic, nutritional and cosmetic. The analyzed data indicate the high relevance of the further introduction of drone brood preparations into nutritional and apitherapeutic practice, which will serve as a tool for correcting the diet of a modern person suffering from a disease of civilization, largely associated with a violation of the ratio and amount of nutrients in the diet.

Dual Antibiotic and Diffusible Signal Factor Combination Nanoliposomes for Combating Staphylococcus epidermidis Biofilm.

Purpose: Microbial biofilms are one of the main causes of persistent human infections. Encapsulation of an antibiotic and a biofilm dispersal agent within a nano-carrier has been recognized as a novel approach to combat the problem of biofilm-related infections. Here, we develop the nanoliposomal formulation for delivery of vancomycin in combination with cis-2- decenoic acid (C2DA), to Staphylococcus epidermidis biofilm. The effects of the formulations were studied at two stages: biofilm growth inhabitation and biofilm eradication. Methods: Liposomal formulations were prepared by the solvent evaporation dehydration-rehydration method and were evaluated for size, zeta potential, and encapsulation efficacy. The ability of different agents in free and encapsulated forms were assessed to evaluate the anti-biofilm activities. Results: Vancomycin and C2DA were successfully co-encapsulated in the same nanoliposome (liposomal combination). The zeta potential values of the liposomal formulations of vancomycin, C2DA, and the liposomal combination were 37.2, 40.2, 51.5 mV, and the mean sizes of these liposomal formulations were 167.8±1.5, 215.5±8.8, 235.5±0.01, respectively. Encapsulation efficacy of C2DA was 65% and about 40% for vancomycin. The results indicated that liposomal combination exerted strong anti-biofilm activities, slightly exceeding those observed by the free form of a combination of vancomycin and C2DA, but higher than either agent used alone in their free forms. The anti-biofilm activity of formulations followed concentration and time-dependent manner. Conclusion: The combination of vancomycin and C2DA could inhibit biofilm formation. Employing the liposomal combination is a considerable method to remove bacterial biofilm.

Chemical Constituents of the Vietnamese Marine Sponge Gelliodes sp. and Their Cytotoxic Activities.

A new decenoic acid derivative, gelliodesinic acid, and a naturally new alkaloid, together with three known furanoterpenoids and two known indole alkaloids, were isolated from the MeOH extract of the marine sponge Gelliodes sp. collected in Vietnam. The chemical structures of the isolated compounds were determined by analyses of 1D- and 2D-NMR and MS data and by comparisons of the data with those reported in the literature. The cytotoxicity assay against HeLa, MCF-7, and A549 cancer cell lines revealed that the three known furanoterpenes exhibited cytotoxic activities with IC50 values ranging from 23.6 to 75.5 μM against the three cell lines, and that 1H-indole-3-carboxylic acid showed cytotoxicity with an IC50 value of 89.2 μM against A549 cancer cell lines.

Identification of characteristic compounds of moderate volatility in breast cancer cell lines.

In this study, we were challenging to identify characteristic compounds in breast cancer cell lines. GC analysis of extracts from the culture media of breast cancer cell lines (MCF-7, SK-BR-3, and YMB-1) using a solid-phase Porapak Q extraction revealed that two compounds of moderate volatility, 1-hexadecanol and 5-(Z)-dodecenoic acid, were detected with markedly higher amount than those in the medium of fibroblast cell line (KMST-6). Furthermore, LC-TOF/MS analysis of the extracts clarified that in addition to the above two fatty acids, the amounts of five unsaturated fatty acids [decenoic acid (C10:1), decadienoic acid (C10:2), 5-(Z)-dodecenoic acid (C12:1), 5-(Z)-tetradecenoic acid (C14:1), and tetradecadienoic acid (C14:2)] in MCF-7 medium were higher than those in medium of KMST-6. Interestingly, H2O2-oxidation of 5-(Z)-dodecenoic acid and 5-(Z)-tetradecenoic acid produced volatile aldehydes that were reported as specific volatiles in breath from various cancer patients, such as heptanal, octanal, nonanal, decanal, 2-(E)-nonenal, and 2-(E)-octenal. Thus, we concluded that these identified compounds over-produced in breast cancer cells in this study could serve as potential precursors producing reported cancer-specific volatiles.

Содержание деценовых кислот в препаратах трутневого расплода и комбинированных препаратах на его основе

The drone brood contains a large number of substances with antioxidant activity. These substances require stabilization and strict adherence to storage conditions. Among these substances are unique decenoic acids, the content of which is an indicator of the quality of drone brood and products based on it. The ability of drone brood to reduce the manifestations of oxidative stress is shown. There are dietary supplements for food and drugs based on drone brood, which are used for a wide range of diseases. Together with drone brood, chitosan-containing products, propolis, royal jelly can be used. They enrich the composition with their own biologically active substances and affect the preservation of the biologically active substances of the drone brood. Promising are the products containing, in addition to the drone brood, a chitin-chitosan-melanin complex from bees, propolis, royal jelly. The chitin-chitosan-melanin complex in the amount of 5% in the composition of the adsorbent practically does not affect the preservation of decenic acids, while in the amount of 2% and 10% it somewhat worsens. The acid-soluble and water-soluble chitosan of marine crustaceans significantly worsens the preservation of decenoic acids in the product. Drone brood with royal jelly demonstrates a rather high content of decenoic acids. When propolis is introduced into the composition of the product, the content of decenoic acids increases according to the content of propolis.

Unravelling the wound healing ability and mode of action of pyridine carboxamide oxime using Caenorhabditis elegans as potential prescreen wound model

AimIn the current scenario of ethical issues related to animal usage in research, the present study was intended to explore the proficient utility of nematode, Caenorhabditis elegans as wound model in preliminary screening of wound healing therapeutics. Main methodsIn this study, a new wounding protocol and quantitative assessment strategies for various healing parameters [survival, Reactive Oxygen Species (ROS), calcium signals, F-actin dynamics, new collagen synthesis and wound induced anti-microbial peptides] were developed and used for preliminary screening of wound healing actives from natural sources. Wound healing ability of positive lead Tridax procumbens (TP) and its major phytocompounds [Octa decenoic acid (ODA), Pyridine carboxamide oxime, known as Nicotinamide (NA) and Dimethyl Benz[c]acridine (DMB)] were assessed using C. elegans wound model and cell lines scratch wound healing assay. Mode of action of active lead was elucidated using metabolome analysis coupled with MALDI-MS followed by molecular docking. Key findingsFrom the four tested methanolic extracts, TP was chosen as positive lead compared to control, Benzalkonium chloride (BKC) based on survival and new collagen synthesis analyses. Results indicated that the wound healing ability of TP was majorly contributed by NA. Further, it was found that NA acts in chloromethyl nicotinamide derivative form by interacting with the known wound healing biomarker, glycogen synthase kinase 3 (GSK-3) to exert wound healing ability. SignificanceThe study evidenced that C. elegans, could be a reliable wound model for high-throughput screening of wound healing actives and to identify their possible mode of action.

Synergistic Anti-Borreliae Efficacy of a Composition of Naturally-occurring Compounds: an In vitro Study

Background: Borrelia sp, which is a pathogenic agent of Lyme diseases in mammals, has become an increasing problem worldwide due to the emergence of persistence. In this study we investigated whether a defined composition of naturally occurring substances could display a broad and synergistic action in vitro against both active and persistent forms of Borrelia spp.Methods: A formulation of six plant-derived compounds combined at their 1/32-1/2 MIC values was tested in vitro against two species of Borrelia recognized as causative agents of Lyme disease in North America and Europe.Results: The results showed that a composition of baicalein, luteolin, rosmarinic acid, monolaurin, cis-2 decenoic acid, and iodine at their 1/8 MIC values has significant synergistic effect against the active and persisting latent forms. This composition revealed anti-oxidative properties affecting Borrelia’s membrane but not DNA. Finally, we observed its inhibitory effect on the release of IL-1α, IL-1β, and IL-6 by human CD14+ monocytes stimulated with live Borrelia sp.Conclusion: These results suggest that such a formulation of compounds might be considered and further explored for its significant pleotropic anti-Borreliae efficacy. Additional in vivo and human studies are warranted to validate this possibility.

Creating functional foodstuffs from high-technological larval raw materials

The possibility of using the apiproducts of the larval origin from the drone larvae in the production of functional foodstuff, such as bakery, confectionery and honey products enriched with high-grade protein, biologically active high-functional substances and complexes that gave them therapeutic and prophylactic, and immune-modulating properties is substantiated in the paper. The recipes for the functional foodstuffs, such as the “Bilar-api” bread, “Pchelka” api-dragee, “Bilar” api-honney, “Bilar” api-honney with bee glue (propolis), enriched with apiproduct from the drone larvae have been developed. To impart an immune-modulating and functional effect to the products, the “Bilar” api-product with a high content of SH-groups, decenoic acids, vitamins, as well as flower pollen, honey, and bee glue in the form of a water-alcohol extract have been used. Preference for these substances has been given because phenolic compounds with P-vitamin activity, unsaturated compounds of isoprene nature, have antioxidant, and fungicidal impacts. They are manifested in their ability to protect the body from the destructive effect of various damaging factors (ionizing radiation, free oxidation radicals, reactive oxygen species, etc.). The “Bilar” powder has a major content of high-grade protein (50-51%), which contains all essential amino acids, and on lysine, tryptophan and histidine exceeds the level of ideal protein on the FAO/WHO scale by almost two times, on the fatty acid composition practically corresponds to the formula of balanced nutrition, vitamins, and decenoic acids (7.9%), as well as sulfhydryl compounds (1160.1 mg/%). Decenoic acid has pronounced antibiotic properties and antitumor effect, exerting an inhibitory effect on the malignant tumors. New functional food products were manufactured on the basis of traditional production technologies using sparing technological regimes and with utilization of apiproducts (the “Bilar” powder, pollen, etc.). Based on the results of the comprehensive study, the increase in the quality and quantity indicators of functional foodstuffs in comparison with control, the increase in biological and nutritional value, as well as improved organoleptic and physics and chemical parameters have been established.

Analysis of the Drosophila melanogaster anti-ovarian response to honey bee queen mandibular pheromone.

Queen mandibular pheromone (QMP) is a potent reproductive signal to which honey bee workers respond by suppressing their ovaries and adopting alloparental roles within the colony. This anti-ovarian effect of QMP on workers can, surprisingly, be induced in other insects, including fruit flies, in which females exposed to synthetic QMP develop smaller ovaries with fewer eggs. In this study, we use the Drosophila melanogaster model to identify the components of synthetic QMP required for the anti-ovarian effect. We found that virgin females respond strongly to 9-oxo-2-decenoic acid and 10-hydroxy-2-decenoic acid (10HDA), suggesting that the decenoic acid components of QMP are essential for the anti-ovarian response. Further, a nuclear factor of activated T-cells reporter system revealed neurones expressing the olfactory receptors Or-56a, Or-49b and Or-98a are activated by QMP in the antenna. In addition, we used olfactory receptor GAL4 drivers and a neuronal activator (a neuronal activating bacterial sodium channel) to test whether the candidate neurones are potential labelled lines for a decenoic acid response. We identified Or-49b as a potential candidate receiver of the 10HDA signal. Finally, the anti-ovarian response to synthetic QMP is not mediated by decreasing the titre of the reproductive hormones ecdysone and juvenile hormone.

Abstract 182: A new anticancer agent derived from decenoic acid for the treatment of colon cancer

Abstract The final goal of the current study is to create a promising lead compound that targets human colon carcinoma. After discovering in vitro antiproliferative activity of a naturally occurring compound, 10-hydroxy-2-decenoic acid, we designed and synthesized over 150 derivatives. The initial screening resulted in the discovery of three primary lead compounds, we then proceeded with modifications in side chain and eventually obtained a second-generation lead compound, named PPI (patented in 2014), whose IC50 was 0.3 μM. We focused on the transcription factor STAT3 that is able to drive carcinogenesis, apoptosis, and angiogenesis-related genes. In silico docking simulation exhibited that PPI can bind to SH2 domain of STAT3, suggesting that this drug blocks dimerization of STAT3, thereby PPI inactivates function of STAT3. PPI inhibited transcriptional activity of STAT3 when SW480, SW837, and HT29 human colon carcinoma cells were treated with 3.7 to 5 μM PPI for 24h. Flowcytometry analysis indicated that when HT29 cells were treated with 1 μM PPI, the percentage of cells in G1 increased by 5% and this was associated with a concomitant decrease of cells in the S and G2-M phases of the cell cycle. The results indicate that PPI causes carcinoma cells to arrest in the G1 phase. When HT29 cells were treated with 10 μM PPI, subG1 fraction and DNA fragmentation were seen, indicating induction of apoptosis. We then performed western blot analysis to determine whether treatment of carcinoma cells with PPI alters cellular levels of the G1 cell cycle control protein cyclin D1 and apoptosis/angiogenesis-related molecules. When HT29 cells were treated with PPI for 24 to 48h, there was a marked decrease in the levels of expression of the Bcl-2, Bcl-xL, and VEGF proteins and a marked increase in the levels of expression of the cleaved caspase 3, 7, 8, 9, and PARP proteins. PPI also inhibited expression levels and nuclear tanslocation of pSTAT3. To see if PPI exerts preventive effect on tumor promotion, F344 rats received sc injections of a carcinogen azoxymethane and then treated with soybean oil or PPI. There was a dose dependent decrease in multiplicity of aberrant crypt foci (ACF) in the colon. PPI inhibited the occurrence of larger ACF consisting of more than 4 aberrant crypts, indicating inhibitory effect of PPI on tumor promotion. In a mouse xenograft model, PPI decreased the size and number of blood vessels in the tumor. All mice survived without causing significant body weight loss during experiment. No histopathological abnormality was found in any of organ site. To confirm the effects of PPI on angiogenesis, we performed chorioallantoic membrane assays and found a dose-dependent decrease in average number of blood vessels. Inhibition of STAT3 by PPI may affect the function of molecules that are related to apoptosis, angiogenesis, and cell cycle progression and eventually contributes to PPI-induced growth inhibition. Citation Format: Masumi Suzui, Saeko Ando, Harutoshi Matsumoto, Katsumi Fukamachi, Mitsuru Futakuchi, Naoki Yoshimi. A new anticancer agent derived from decenoic acid for the treatment of colon cancer [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2017; 2017 Apr 1-5; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2017;77(13 Suppl):Abstract nr 182. doi:10.1158/1538-7445.AM2017-182