Decabrominated Diphenyl Ether Research Articles

DNA methylation changes induced by BDE-209 are related to DNA damage response and germ cell development in GC-2spd

Decabrominated diphenyl ether (BDE-209) is generally utilized in multiple polymer materials as common brominated flame retardant. BDE-209 has been listed as persistent organic pollutants (POPs), which was considered to be reproductive toxin in the environment. But it still remains unclear about the effects of BDE-209 on DNA methylation and the induced-male reproductive toxicity. Due to the extensive epigenetic regulation in germ line development, we hypothesize that BDE-209 exposure impacts the statue of DNA methylation in spermatocytes in vitro. Therefore, the mouse GC-2spd (GC-2) cells were used for the genome wide DNA methylation analysis after treated with 32 μg/mL BDE-209 for 24 hr. The results showed that BDE-209 caused genomic methylation changes with 32,083 differentially methylated CpGs in GC-2 cells, including 16,164 (50.38%) hypermethylated and 15,919 (49.62%) hypomethylated sites. With integrated analysis of DNA methylation data and functional enrichment, we found that BDE-209 might affect the functional transcription in cell growth and sperm development by differential gene methylation. qRT-PCR validation demonstrated the involvement of p53-dependent DNA damage response in the GC-2 cells after BDE-209 exposure. In general, our findings indicated that BDE-209-induced genome wide methylation changes could be interrelated with reproductive dysfunction. This study might provide new insights into the mechanisms of male reproductive toxicity under the environmental exposure to BDE-209.

Aerobic degradation of decabrominated diphenyl ether through a novel bacterium isolated from municipal waste dumping site: Identification, degradation and metabolic pathway

In the present study, a novel bacterium capable of degrading BDE-209 aerobically was isolated from a municipal waste dumping site and identified as Bacillus tequilensis strain BDE-S1 through 16S rRNA gene sequencing. A correlation between BDE-209 and bromide concentration, COD, TOC, and cell biomass was established. 65% of 50 mg/L initial concentration of BDE-209 was degraded within eight days of incubation by BDE-S1 strain. Two hexa, two penta, one tetra-BDE congener, and benzamide were detected as metabolites. The bromide release, COD, TOC and cell biomass were found to be significantly correlated parameters with BDE-209 degradation. Based on the metabolite analysis, ortho and meta debromination, cleavage of diphenyl ether bond and ring-opening were suggested as possible degradation pathways. This is the first study demonstrating the use of indigenously isolated Bacillus tequilensis strain BDE-S1 for aerobic degradation of BDE-209, which could provide new comprehension for bioremediation of PBDEs from contaminated environments.

Effects of Decabrominated Diphenyl Ether Exposure on Growth, Meat Characteristics and Blood Profiles in Broilers.

Simple SummaryDecabrominated diphenyl ether (BDE-209) has been widely used in recent decades, and its cumulative toxicity to crops, animals and human beings is attracting increasing attention. In this study, we established broiler models to investigate the effects of BDE-209 exposure at doses of 0, 0.02, 0.4 and 4 mg/kg on growth, carcass traits, meat quality, blood profiles and antioxidant capacity. Our results suggested that BDE-209 exposure altered the blood profiles and carcass traits and exhibited toxicity in liver and kidney functions. Furthermore, BDE-209 increased plasma malondialdehyde (MDA) concentrations and decreased the activities of glutathione peroxidase (GSH-px) and superoxide dismutase (SOD), which implied aggravating oxidant stress and decline of antioxidant capacity in broilers.Decabrominated diphenyl ether (BDE-209) is widely used as a flame retardant and is detected at high levels in the environment. Its toxicities have been reported and have attracted attention. In the present study, broilers were used to determine the response in growth performance, carcass traits, meat quality, blood profiles and antioxidant system to BDE-209 exposure at doses of 0, 0.02, 0.4 and 4 mg/kg. The results showed that BDE-209 exposure at levels of 0.02 or 0.4 mg/kg increased feed intake and decreased feed efficiency. BDE-209 altered the blood profiles, such as reducing the numbers of white blood cells, lymphocytes and neutrophilic granulocytes. As compared with the control, BDE-209 exposure significantly increased abdominal fat percentages of broilers at 64.9–159.5% and adversely affected the selected biochemical indicators, including alkaline phosphatase (ALP), aspartate aminotransferase (AST), alanine aminotransferase (ALT), creatine (CRE), which indicated its toxicity to liver and kidney functions. Moreover, BDE-209 exposure significantly increased plasma malondialdehyde (MDA) concentrations and decreased the activities of glutathione peroxidase (GSH-px) and superoxide dismutase (SOD), which implied aggravating oxidant stress and decline of antioxidant capacity in broilers. In conclusion, our data demonstrated that the environmental pollutant BDE-209 adversely influenced growth performance, increased the deposition of abdominal fat, impaired antioxidant capacity and the immune system and had potential toxicity to the liver and kidney of broilers.

Toxicokinetics and Edible Tissues-Specific Bioaccumulation of Decabrominated Diphenyl Ethers (Bde-209) after Exposure to the Broilers

Toxicokinetics and Edible Tissues-Specific Bioaccumulation of Decabrominated Diphenyl Ethers (Bde-209) after Exposure to the Broilers

BDE-209 and DBDPE induce male reproductive toxicity through telomere-related cell senescence and apoptosis in SD rat

Decabrominated diphenyl ether (BDE-209) and decabromodiphenyl ethane (DBDPE) are common flame retardants utilized in many kinds of electronic and textile products. Due to their persistence and bioaccumulation, BDE-209 and DBDPE extensively exist in the surrounding environment and wild animals. Previous studies have indicated that BDE-209 could induce male reproductive toxicity, whereas those of DBDPE remains relatively rare. In this study, we investigated the effects of both BDE-209 and DBDPE on reproductive system in male SD rats, and explored the potential mechanisms under the reproductive toxicity of BDE-209 and DBDPE. Male rats were orally administered with BDE-209 and DBDPE (0, 5, 50 and 500 mg/kg/day) for a 28-day exposure experiment. The current results showed that BDE-209 and DBDPE led to testicular damage in physiological structure, decreased the sperm number and motility, and increased the sperm malformation rates in rat. Moreover, BDE-209 and DBDPE could damage the telomeric function by shortening telomere length and reducing telomerase activity, which consequently caused cell senescence and apoptosis in testis of rat. This could contribute to the decline of sperm quality and quantity. In conclusion, BDE-209 and DBDPE led to reproductive toxicity by inducing telomere dysfunction and the related cell senescence and apoptosis in testis of SD rat. Comparatively, BDE-209 had more severe effects on male reproduction. Our findings may provide new insight into the potential deleterious effects of BFRs on male reproductive health.

Nephrotoxicity and possible mechanisms of decabrominated diphenyl ethers (BDE-209) exposure to kidney in broilers

The flame retardant decabrominated diphenyl ether (BDE-209) is a widely used chemical in a variety of products and exists extensively in the environment. BDE-209 has been reported to induce kidney injury and dysfunction. However, the causes and mechanisms of its nephrotoxicity are still under investigation. In this study, 150 male broilers were exposed to BDE-209 concentrations of 0, 0.004, 0.04, 0.4, 4.0 g/kg for 42 days. The relative kidney weight, histopathology, markers of renal injury, oxidative stress, inflammation, apoptosis and the expression of MAPK signaling pathways-related proteins were assessed. The results showed that the concentrations of blood urea nitrogen (BUN), creatinine (CRE) and the neutrophil gelatinase-associated lipocalin (NGAL), significantly increased after exposure to BDE-209 with the doses more than 0.04 g/kg. Similarly, severe damage of renal morphology was observed, including atrophy and necrosis of glomeruli, and swelling and granular degeneration of the renal tubular epithelium. In the renal homogenates, the oxidative stress was evidenced by the elevated concentrations of MDA and NO, and decreased levels of GSH-Px, GSH and SOD. Due to the inflammatory response, the level of NF-κB and the pro-inflammatory cytokines TNF-α, IL-1β, IL-18 were remarkably upregulated, while the content of the anti-inflammatory cytokine IL-10 decreased. Additionally, the apoptotic analysis showed notable upregulations of Bax/Bcl-2 ratio, the relative expression of p-ERK1/2 and p-JNK1/2, and the expression of Bax, cytochrome c and caspase 3. The present study indicates that BDE-209 exposure can cause nephrotoxicity in broilers through oxidative stress and inflammation, which activate the phosphorylation of key proteins of the MAPK signaling pathways, and subsequently induce mitochondria-mediated kidney apoptosis.

Serum levels of novel brominated flame retardants (NBFRs) in residents of a major BFR-producing region: Occurrence, impact factors and the relationship to thyroid and liver function

Five currently used novel brominated flame retardants (NBFRs) were determined in 172 serum samples collected from nonoccupational residents of a major BFR-producing region. All the 5 NBFRs presented high detection frequencies (DFs, >90%), and decabromodiphenyl ethane (DBDPE), a substitute of decabrominated diphenyl ethers (deca-BDE), was the most abundant NBFR. The levels of DBDPE were from <LOD to 1590 ng/g lw, with a median level of 32.5 ng/g lw. The median levels of other NBFRs were from 0.134 to 2.87 ng/g lw, which were at least 10 times lower than that of DBDPE. Moreover, a comparison to other studies showed that our results were significantly higher than studies conducted in background population. The levels of some NBFRs adjusted by serum lipid showed negative and significant correlation with BMI, whereas the difference disappeared when NBFRs levels were calculated based on serum volume. Certain NBFRs in female showed significantly higher concentrations than those in male. No significant effect of age, smoking habit, education level and children birth (in female) on serum NBFR levels was observed. The relationship between the serum levels of NBFRs and a series of thyroid/liver injury biomarkers was further analyzed to evaluate the health effects of these NBFRs to human being. Results showed that a 10-fold increment in the serum DBDPE level was associated with decreased total triiodothyronine (TT3) level (−0.037 nmol/L) [95% CI: −0.070, −0.003]， whereas serum pentabromoethylbenzene (PBEB) level was associated with increased total triiodothyronine (TT3) level (0.031 nmol/L) [95% CI: 0.001, 0.060]. For liver indicators, a 10-fold increment in the serum level of PBT was associated with decreased Ln aspartate aminotransferase/alanine aminotransferase (AST/ALT) level (−0.068) [95% CI: −0.129, −0.007]. A 10-fold increment in the serum level of BTBPE was associated with increased TBIL level (0.869 μmol/L) [95% CI: 0.175, 1.564], direct bilirubin (DBIL) level (0.231 μmol/L) [95% CI: 0.075, 0.388] and IDBIL level (0.638 μmol/L) [95% CI: 0.091, 1.185]. Our findings indicate that BFR production is posing heavy BFR contamination to surrounding environment and human being, and which might relate to thyroid disruption and liver injury.

A coupled UV photolysis-biodegradation process for the treatment of decabrominated diphenyl ethers in an aerobic novel bioslurry reactor.

The commercial flame retardant is an emerging contaminant (EC) commonly found in soils and sediments. A coupled UV-photolysis-biodegradation process was used to decompose decabromodiphenyl ether (BDE-209) in clay slurries. A novel bioslurry bioreactor (NBB) was employed in which BDE-209 degradation was maximized by the simultaneous application of LED UVA irradiation and biodegradation by a mixed bacterial culture. The rate of BDE-209 degradation decreased in the order: coupled UV photolysis-biodegradation (1.31 × 10-2 day-1) > UV photolysis alone (1.10 × 10-2 day-1) > biodegradation alone (1.00 × 10-2 day-1). Degradation intermediates detected included hydroxylated polybrominated diphenylethers, partially debrominated PBDE congeners and polybrominated dibenzofuran. The UV-resistant bacterial strains isolated that could utilize BDE-209 as a sole carbon source included Stenotrophomonas sp., Pseudomonas sp., and Microbacterium sp. These strains encoded important functional genes such as dioxygenase and reductive dehalogenases. Continuous UV irradiation during the NBB process affected various biochemical oxidative reactions during PBDEs biodegradation. Simultaneous photolysis and biodegradation in the NBB system described reduces operational time, energy, expense, and maintenance-demands required for the remediation of BDE-209 when compared to sequential UV-biodegradation process or to biodegradation alone.Electronic supplementary materialThe online version of this article (10.1007/s11356-020-10753-9) contains supplementary material, which is available to authorized users.

Enhancement of microbial redox cycling of iron in zero-valent iron oxidation coupling with deca-brominated diphenyl ether removal

Iron-redox cycling microorganisms are important for understanding the biogeochemical iron and play key roles in zero-valent iron (ZVI) mediated environmental bioremediation. Their influence on ZVI oxidation coupling with organic contaminant removal is of particular interest but is still poorly understood. The objective of this research was to study microbial redox cycles of iron in ZVI oxidation and deca-brominated diphenyl ether (deca-BDE) removal. It was found that iron-oxidizing bacteria (IOB) enhanced ZVI oxidation by using iron as the sole electron donor. Iron-reducing bacteria (IRB) with high activity of Fe (III) reduction, also significantly accelerated rather than inhibited ZVI oxidation. ZVI oxidation activity was increased from 3.42% to 24.28% by IOB and 19.49% by IRB. When deca-BDE was present in the medium, ZVI oxidation activity by IOB and IRB was increased from 2.67% to 48.33% and 64.33%, respectively. However, no co-accelerating effect of IOB and IRB occurred but rather a neutralizing influence on ZVI oxidation was detected with iron-redox cycling bacteria (IORB). ZVI oxidation activity by IORB only increased to 13.14% and 37.0% in the absence and presence of deca-BDE, respectively. Meanwhile, IRB also exhibited the highest removal activity of deca-BDE. Approximately 71.67% of deca-BDE was removed by IRB, compared to 18.91% by IOB and 43.24% by IORB. Deca-BDE significantly influenced the effects of iron-metabolizing microorganisms on ZVI oxidation by altering the composition of microbial communities. Pseudomonas, Paenibacillus, and Sporolactobacillus were the key genera influencing ZVI oxidation and deca-BDE removal. Sporolactobacillus was firstly reported to be able to stimulate both ZVI oxidation and deca-BDE removal. Pseudomonas accelerated ZVI oxidation but had no significant contribution to deca-BDE removal. However, Paenibacillus inhibited both Fe(III) reduction and deca-BDE removal. It is expected that continuous integration of ZVI oxidation and organic contaminant removal can be achieved by regulating the key genera in iron-metabolizing microbial communities.

Transportation and degradation of decabrominated diphenyl ether in sequential anoxic and oxic crop rotation

This work evaluated the debromination and uptake of 14C-labeled BDE-209 in rice cultivars grown in anoxic soil for 120 days (d) followed by cultivation of vegetables (peanut, eggplant and pepper) in oxic soil (120 d). Degradation of BDE-209 to lower polybrominated diphenyl ethers (PBDEs) occurred in cultivated soils, and more metabolites were released in oxic soil than in anoxic soil. The crop rotation from anoxic to oxic greatly enhanced the dissipation of BDE-209 in the soil (P < 0.05), in which the dissipation in anoxic soil planted with Huanghuazhan (HHZ, indica) and Yudao 1 (YD1, indica) were 6.8% and 2.4%, respectively, while in oxic soil with peanut and pepper were increased to 25.8% and 21.7%, respectively. The crop rotation also enhanced the degradation of BDE-209 in the soil, the recovered BDE-209 in soil after 120 d anoxic incubation with YD1 was 81.1%, but it decreased to 47.8% and 45.8% after another 120 d oxic incubation. Bioconcentration factors were between 0.23 and 0.36 for rice, eggplant and pepper but reached to 0.5 in peanut, which contains more lipids in the edible portion than the other test crops. The estimated daily intake for vegetables was 0.01–0.07 μg BDE-209-equivalent kg−1 bw day−1, which is at least two orders of magnitude below the maximum acceptable oral dose (7 μg kg−1 bw day−1). Our work confirms that crop rotation from rice to vegetable enhanced the dissipation and debromination of BDE-209 in the soil, and indicate that sequential anoxic-oxic rotation practice is considered to be effective in remediation of environmental pollutants.

Polybrominated diphenyl ethers and decabromodiphenyl ethane in paired hair/serum and nail/serum from corresponding chemical manufacturing workers and their correlations to thyroid hormones, liver and kidney injury markers

We detected the polybrominated diphenyl ethers (PBDEs) or decabromodiphenyl ethane (DBDPE) in paired hair-serum and nail-serum samples collected from the corresponding chemical manufacturing workers. The levels of decabrominated diphenyl ether (BDE-209) or DBDPE in the serum, hair and nail samples were all significantly higher than those reported in other studies, and the “work place” (pretreatment or posttreatment workshop) was the primary influencing factor that affected the levels of specific BFRs in vivo. For BDE-209 workers, the BDE-209 in both the hair and nail samples were significantly and positively related to the BDE-209 in the serum, indicating that both hair and nails can be used as noninvasive biomatrices to reflect internal exposure to BDE-209. In DBDPE workers, hair rather than nails was more suitable for use as a noninvasive biomatrix to infer the DBDPE exposure level. A series of serum biomarkers reflecting thyroid hormones and liver and kidney injuries were tested to calculate the correlations between hair or nail BFR levels and the levels of the biomatrices. The BDE-209 in the hair samples was significantly and positively correlated with the total protein (TP), and the nail BDE-209 level was significantly and positively related to the total bilirubin (TBIL), indirect bilirubin (IDBIL) and uric acid (UA). The DBDPE in hair was significantly and positively correlated with the thyroid hormones free triiodothyronine (fT3) and total triiodothyronine (tT3) and kidney injury markers, including blood urea nitrogen (BUN), creatinine (CRE) and cystatin C (Cys-C). In addition, the nail DBDPE levels were significantly and positively correlated with the albumin/globulin (A/G), BUN, CRE and Cys-C but negatively correlated with the TP and globulin (GLO). Our findings provide preliminary evidence that hair and nails can be used as noninvasive biomatrices for assessing internal BFR exposure and health damage in occupational workers.

Characterization of a Sequential UV Photolysis-Biodegradation Process for Treatment of Decabrominated Diphenyl Ethers in Sorbent/Water Systems.

Decabrominated diphenyl ether (BDE-209) is a primary component of the brominated flame retardants used in a variety of industrial and domestic applications. BDE-209 bioaccumulates in aquatic organisms and has been identified as an emerging contaminant that threatens human and ecosystem health. Sequential photolysis-microbial biodegradation processes were utilized here to treat BDE-209 in clay- or soil-water slurries. The removal efficiency of BDE-209 in the clay-water slurries was high; i.e., 96.5%, while that in the soil-water slurries was minimal. In the clay-water slurries the first order rate constants for the UV photolysis and biodegradation of BDE-209 were 0.017 1/day and 0.026 1/day, respectively. UV wavelength and intensity strongly influenced the BDE-209 photolysis and the subsequent biodegradation of photolytic products. Facultative chemotrophic bacteria, including Acidovorax spp., Pseudomonas spp., Novosphingobium spp. and Sphingomonas spp., were the dominant members of the bacterial community (about 71%) at the beginning of the biodegradation; many of these organisms have previously been shown to biodegrade BDE-209 and other polybrominated diphenyl ether (PBDE) congeners. The Achromobacter sp. that were isolated (NH-2; NH-4; NH-6) were especially effective during the BDE-209 degradation. These results indicated the effectiveness of the sequential UV photolysis and biodegradation for treating certain BDE-209-contaminated solids; e.g., clays; in bioreactors containing such solids as aqueous slurries. Achieving a similar treatment effectiveness for more heterogeneous solids containing natural organic matter, e.g., surface solids, appears to be significantly more difficult. Further investigations are needed in order to understand the great difference between the clay-water or soil-water slurries.

Dietary exposure assessment of a nursing mother-infant cohort to legacy and novel brominated flame retardants: Results of a 3-day duplicate diet study in Beijing, China

In a 3-day duplicate diet study of a nursing mother-infant cohort (n = 20), the levels of polybrominated diphenyl ethers (PBDEs), hexabromocyclododecane (HBCDD), and 5 novel brominated flame retardants (BFRs) were measured in 60 24-h duplicate diet samples and 20 breast milk samples provided by the mothers. The dietary BFR intake and related health risks of the mothers and their babies due to food consumption or human milk ingestion were subsequently assessed. At median concentrations of 284, 264 and 177 pg/g wet weight (ww) in the diet, decabrominated diphenyl ethers (BDE-209), decabromodiphenyl ethane (DBDPE) and HBCDD were predominant among the total BFRs. In human milk, HBCDD was the most abundant BFR, followed by BDE-209 and DBDPE, which indicates that BDE-209 and HBCDD have remained ubiquitous in the environment because of their continuous production and use in China. Meanwhile, concentrations of DBDPE were comparative to those of PBDEs and HBCDD in both diet and human milk, and DBDPE also had much higher concentrations than any other NBFRs, which indicates that the BFR consumption pattern in China is shifting from legacy BFRs to NBFRs. The median estimated dietary intakes (EDIs) of BDE-209, HBCDD and DBDPE for the mothers were 6.83, 3.73 and 5.44 ng/kg bw/day, respectively, and EDIs for their nursing babies were 24.7, 41.9 and 7.83 ng/kg bw/day, respectively. The nursing infants showed higher BFR body burden than the mothers. However, the EDIs obtained for both mothers and their babies discloses a low health risk to this mother-infant cohort.

Compound-specific carbon isotope analysis for mechanistic characterization of debromination of decabrominated diphenyl ether.

Decabrominated diphenyl ether (BDE-209) is a notorious persistent organic pollutant widely found in the environment. Developing a compound-specific isotope analysis (CSIA) method is much needed in order to trace its transport and degradation processes and to evaluate the effectiveness of the remediation of BDE-209 in the environment. However, the conventional CSIA method, i.e. gas chromatography (GC) combustion isotope ratio mass spectrometry, is not appropriate for BDE-209 because of its high thermal instability and incomplete combustion. We developed a high-performance liquid chromatography (HPLC) method for the separation and purification of BDE-209 that prevents its thermal reactivity as occurred in prior GC-based methods. The δ13 C value of the purified BDE-209 was determined using offline elemental analyzer isotope ratio mass spectrometry (EA/IRMS). This two-step method was applied to determine the δ13 C values of BDE-209 in two commercial samples and to characterize carbon isotope fractionation associated with the debromination of BDE-209 via nanoscale zero-valent iron. The mean values of daily δ13 C analyses of six replicates of a BDE-209 standard varied from -27.66‰ to -27.92‰, with a standard deviation ranging from 0.07‰ to 0.16‰, indicating a good reproducibility of EA/IRMS. The EA/IRMS analysis of the purified BDE-209 standard indicated no obvious isotope fractionation during the sample purification. The impurity content in commercial BDE-209 samples may contribute additional variation of the δ13 C values of BDE-209. The δ13 C values of BDE-209 gradually changed from -27.47 ± 0.37‰ to -24.59 ± 0.19‰ when 74% of the BDE-209 standard was degraded within 36 h. The estimated carbon isotope enrichment factor was -1.72 ± 0.18‰. The two-step method based on HPLC and EA/IRMS avoids the thermal instability of BDE-209 in the traditional CSIA method. It offers a novel approach for elucidating the degradation mechanisms of BDE-209 in the environment and for source identification in contaminated sites.

New insights into the anaerobic microbial degradation of decabrominated diphenyl ether (BDE-209) in coastal marine sediments

Severe contamination of decabrominated diphenyl ether (BDE-209, an emerging persistent organic pollutant) in coastal marine sediments has posed a serious threat to the marine ecosystems. Anaerobic microbial degradation can affect the toxicity and environmental fate of BDE-209 in anoxic marine sediments. However, little is known about the anaerobic microbial degradation of BDE-209 in anoxic marine/coastal sediments. In this study, the anaerobic degradation of BDE-209 in microcosms containing coastal marine sediments from a contaminated bay located in Southern China was investigated. It was observed that over 70% of the BDE-209 (5 μmol) added to the anaerobic sediment microcosms disappeared after 90-day of incubation. Thirty-five debrominated products (tetra- to nonaBDEs) were identified by GC-MS. Remarkably, a majority of these products (i.e. 20 products, including BDE-52, -92, −101, −102, −103, −133, −144, −146, −150, −161, −171, −172, −175, −177, −178, −180, −182, −188, −193, −199) have not been previously reported in the literature on the anaerobic microbial degradation of BDE-209 in sediments. There was no preferential debromination among ortho-, meta-, and para-bromines on BDE-209 and higher-brominated diphenyl ethers were the predominant debromination products. High-throughput sequencing revealed that the relative abundances of 9 microbial genera in the sediment microcosms increased as the anaerobic degradation of BDE-209 progressed, indicating their involvements in the degradation process. Taken together, our findings provided new insights into the anaerobic microbial degradation of BDE-209 in anoxic marine sediments.

Determination of Polybrominated Diphenyl Ethers (PBDEs) in Freshwater Fish Around a Deca-brominated Diphenyl Ether (deca-BDE) Production Facility by Gas Chromatography-Mass Spectrometry (GC-MS)

Polybrominated diphenyl ethers (PBDEs) were determined in 36 fish samples near a deca- brominated diphenyl ether (deca-BDE) production facility in Weifang City, Shandong Province, China. The concentrations of PBDEs in fish muscle and liver samples were from 45.8 to 560 ng g−1 lipid weight (lw) and 327 to 2120 ng g−1, lw, respectively. The highest concentration of PBDEs was found in carp with a mean value of 1370 ng g−1, lw, followed by crucian with a mean of 1030 ng g−1, lw, and grass carp with a mean of 681 ng g−1, lw. Relatively constant congener patterns were observed among the fish species. BDE-47 was the most predominant congener with contributions of 50.1% to 89.7% to the total PBDE concentrations. Different BDE-99/BDE-100 ratios were observed, indicating that the PBDE pattern is species-dependent.

Temporal and spatial surveys of polybromodiphenyl ethers (PBDEs) contamination of soil near a factory using PBDEs in northern Taiwan

Polybrominated diphenyl ethers (PBDEs), previously commonly used as flame retardants, should be monitored in the environment since some are listed as persistent organic pollutants. A contaminated site near a northern Taiwan factory using decabrominated diphenyl ether (deca-BDE) was identified based on a vegetable PBDEs monitoring project in 2013. The subsequent spatial and temporal survey of that contaminated site shows the contamination ingredients in soils were close to ones used by the factory, indicating that contamination was from the factory, possibly through an exhaust vent. The average concentration of deca-BDE in the main contaminated soil was 615 μg/kg d. w. (dry weight) soil in 2015, slightly decreasing to 604 μg/kg d. w. soil in 2016, increasing to 844 μg/kg d. w. soil in 2017, and then slightly decreasing to 670 μg/kg d. w. soil in 2018. The slight change of deca-BDE and the minor change in low brominated congener level indicate a low degradation rate. The contamination of peripheral sites was around 5000 μg/kg d. w. soil for one PBDEs sampling site that was higher than those around or within the main contaminated farm, indicating serious pollution. Concentrations of PBDEs in different soil depths show that depth 2–15 cm accounted for the greatest PBDEs accumulation, indicating that deca-BDE pollution had been present over time and transported into deeper soil. There can be PBDEs uptake by crops consumed by humans, as shown in our previous studies, so continuous monitoring of PBDEs in this site is important and treatments should be established urgently.

Bioconcentration, Biotransformation, and Thyroid Endocrine Disruption of Decabromodiphenyl Ethane (Dbdpe), A Novel Brominated Flame Retardant, in Zebrafish Larvae.

The brominated flame retardant decabromodiphenyl ethane (DBDPE), an alternative to decabrominated diphenyl ether (BDE209), has become a widespread environmental contaminant, but its possible toxic effects to wildlife remain unknown. Using zebrafish as a model, we investigated the bioconcentration and impact of DBDPE on thyroid endocrine function after water-borne exposure, compared to BDE209. Zebrafish embryos were exposed to DBDPE or BDE209 (0, 3, 10, 30, 100, 300 nM) for 6 or 14 days. Chemical analysis revealed that DBDPE and BDE209 were bioconcentrated in zebrafish larvae, with similar magnitudes of accumulated concentrations. Based on screened by chromatograms, at least seven unknown compounds were observed in DBDPE-treated larvae, indicating biotransformation of the chemical. Significant increases in whole body content of triiodothyronine (T3) and thyroxine (T4) were detected in DBDPE-treated larvae, but decreased in BDE209-treated groups. Alterations in gene transcription along the related hypothalamic-pituitary-thyroid (HPT) axis were observed. Furthermore, the binding and transport protein transthyretin (TTR) was significantly increased in DBDPE exposure groups. Histological examination and stereological analysis showed no obvious pathological changes in the thyroid gland. The present study demonstrates for the first time the bioavailability, biotransformation and thyroid endocrine disruption associated with DBDPE exposure in fish. Further studies are warranted to identify the metabolites of DBDPE and to define its environmental risks to aquatic organisms.

Dynamic stock, flow, and emissions of brominated flame retardants for vehicles in Japan

The transportation industry, including the automobile industry, is one of the primary manufacturing sectors that have used polybrominated diphenyl ethers (PBDEs) and hexabromocyclododecane (HBCD) within interior components. In this study, a dynamic substance flow model and scenario analysis were used to analyze the trends of PBDE and HBCD stocks, and the flow and emissions from vehicles were estimated. Under the strict elimination scenario, the average amount of commercially decabrominated diphenyl ether (c-DecaBDE) was 62 ± 21 g per conventional passenger vehicle (CPV) and 38 ± 13 g per miniature passenger vehicle (Mini PV). The average amount of HBCD was 3 ± 2 g per CPV and 2 ± 1 g per Mini PV. Seat fabric contributed ∼87% of the c-DecaBDE, while floor covering contributed ∼73% of the HBCD for passenger vehicles (PVs). For vehicles in use, the amount of c-DecaBDE was ∼2800–3100 tonnes from fiscal year 1998 (FY1998), while that of HBCD was ∼120–130 tonnes from FY2002; this remained stable until elimination began. Scenario analysis revealed that at least 5000–6700 tonnes of c-DecaBDE and 260–280 tonnes of HBCD entered in domestic PVs use could be avoided through elimination measures taken by Japanese vehicle manufacturers. The flows of c-DecaBDE and HBCD in FY2003 and FY2013 also demonstrated the effectiveness of elimination measures. However, approximately 300 ± 100 tonnes of c-DecaBDE and 1.8 ± 1.1 tonnes of HBCD will still be stocked in vehicles in FY2030. The atmosphere is the main environmental media for c-DecaBDE and HBCD emissions, and the usage phase is the most important stage. The fate of brominated flame retardants (BFRs) during and after material recycling should be carefully monitored and managed because the end-of-life vehicle treatment stage may become the dominant phase of BFR emissions with the decline of c-DecaBDE and HBCD stocked in vehicles.

Inhibition of progesterone biosynthesis induced by deca-brominated diphenyl ether (BDE-209) in mouse Leydig tumor cell (MLTC-1)

Polybrominated Diphenyl Ethers (PBDEs) have been extensively applied as flame retardants in different polymeric materials since the 1970s, which have become a group of long-lasting environmental pollutants. They have been reported from previous studies to accumulate and then disrupt the endocrine system in humans. However, the mechanisms are still little known. In the present study, mouse Leydig tumor cells were utilized to investigate steroidogenic activity influenced by deca-brominated diphenyl ether (BDE-209). Our data showed that BDE-209 did not change intracellular cAMP level in the presence of human Chorionic Gonadotropin (hCG), cholera toxin (CT), and forskolin, which indicated that reduction of progesterone may not be related to the hCG-cAMP signal pathway in MLTC-1 cells. Furthermore, the reduction of progesterone generation was not shifted by 8-Br-cAMP, an analog of cAMP, indicating that BDE-209 may inhibit post-cAMP sites. In addition, mRNA expression levels of P450 side-chain cleavage enzyme (P450scc) and 3β-hydroxysteroid dehydrogenase (3β-HSD) presented a concentration-dependent decrease. In conclusion, this study suggested that BDE-209 may attenuate the progesterone secretion mainly through lowering the expression of these two enzymes.