To examine the differences in muscle fatigability after resistance exercise performed with fast tempo (FT) compared with slow tempo (ST). A total of 8 resistance-trained males completed FT and ST hexagonal-barbell deadlifts, consisting of 8 sets of 6 repetitions at 60% 3-repetition maximum, using a randomized crossover design. Each FT repetition was performed with maximal velocity, while each repetition during ST was performed with a 3-1-3 (eccentric/isometric/concentric) tempo (measured in seconds). Isometric maximal voluntary contraction, voluntary muscle activation, and evoked potentiated twitch torque of the knee extensors were determined using twitch interpolation before, during (set 4), and after exercise. Displacement-time data were measured during the protocols. The mean bar velocity and total concentric work were higher for FT compared with ST (995 [166] W vs 233 [52]W; 0.87 [0.05]m/s vs 0.19 [0.05]m/s; 4.8 [0.8]kJ vs 3.7 [1.1]kJ). Maximal voluntary contraction torque, potentiated twitch, and voluntary muscle activation were significantly reduced after FT (-7.8% [9.2%]; -5.2% [9.2%], -8.7% [12.2%]) and ST (-11.2% [8.4%], -13.3% [8.1%], -1.8% [3.6%]). The decline in maximal voluntary force after both the FT and ST hexagonal-barbell deadlifts exercise was accompanied by a similar decline in contractile force and voluntary muscle activation.