This article presents an experimental study of a recently developed process, namely, ultrasonic assisted jet electrochemical micro-drilling (UAJet-ECMD) using pulsed DC voltage power supply. The goal of the work was to examine the effect of pulsed DC voltage on the performance of UAJet-ECMD process. In the previous work carried out by the authors, the process has been studied using the continuous DC voltage. The pulse “on” time (pulsed DC voltage), electrolyte pressure and pulse “on” time (ultrasonic vibrations) were selected as the process parameters, whereas material removal rate (MRR) and hole taper were chosen as process responses. It was found that the pulse “on” time (pulsed DC voltage) had crucial effect on the MRR as well as on the hole taper. MRR and hole taper were both found to increase with rise in pulse “on” time (pulsed DC voltage). Optimization of process responses of UAJet-ECMD process was done. The responses obtained for optimized set of process parameters were verified and found in good conformity with the experimental results.