Tricholoma matsutake (S. Ito & Imai) Singer is most expensive edible mushroom, naturally grown dispersed in temperate oak pine forested areas in Bhutan. It is ectomycorrhizal fungi with high ecological and economic value requiring an important ecological niche and symbiotic tree associates. The present study is an attempt to give an account on floristic composition and vegetation structure of Tricholoma matsutake habitat in Genekha, Thimphu. A total of 10 plots were enumerated with plot size of 20m X 20m, 5m X 5m and 2m X 2m for trees, shrubs and herbs respectively. PAST (Paleontological Statistics) 4.10 software was used for diversity analysis. A total of 58 species under 44 genera belonging to 26 families were recorded in the natural habitat of matsutake. The study revealed that T. matsutake is associated with Quercus semecarpifolia Sm., Pinus wallichiana A.B. Jack., Rhododendron spp., and Pieris formosa (Wall.) D. Don. J-shaped distribution curve of DBH and height class with fair regeneration status is obtained for associated species in the habitat. The Menhinick’s species richness of (2.26, 2) for both North facing habitat (NFH) and South facing habitats (SFH) indicated high species richness. Moderate and low diversity is indicated with Simpsons index 0.94 (NFH) and 0.76 (SFH), Shannon index 3.06 (NFH) and 2.49 (SFH) with moderately even distribution 0.74(NFH) and 0.73 (SFH) of species in the habitats. Sorensens similarity index of 0.9 indicated highly similar species composition between two habitats. This research provides current floristic and vegetation structure of Tricholoma matsutake habitat, that will have high significance in habitat management and conservation.
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