Daily Samples Research Articles

Diagnostic performance and longitudinal analysis of fungal biomarkers in COVID-19 associated pulmonary aspergillosis

ObjectivesGalactomannan lateral flow assay (GM-LFA) is a reliable test for COVID-19 associated pulmonary aspergillosis (CAPA) diagnosis. We aimed to assess the diagnostic performance of GM-LFA with different case definitions, the association between the longitudinal measurements of serum GM-ELISA, GM-LFA, and the risk of death. MethodsSerum and nondirected bronchial lavage (NBL) samples were periodically collected. The sensitivity and specificity analysis for GM-LFA was done in different time periods. Longitudinal analysis was done with the joint model framework. ResultsA total of 207 patients were evaluated. On the day of CAPA diagnosis, serum GM-LFA had a sensitivity of 42 % (95 % CI: 23–63) and specificity of 82 % (95 % CI: 78–84), while NBL GM-LFA had a sensitivity of 73 % (95 % CI: 45–92), specificity of 85 % (95 % CI: 76–91) for CAPA. Sensitivity decreased through the following days in both samples. Univariate joint model analysis showed that increasing GM-LFA and GM-ELISA levels were associated with increased mortality, and that effect remained same with serum GM-ELISA in multivariate joint model analysis. ConclusionGM-LFA, particularly in NBL samples, seems to be a reliable method for CAPA diagnosis. For detecting patients with higher risk of mortality, longitudinal measurement of serum GM-ELISA can be useful.

Prevalence of red panda amdoparvovirus infection in European zoos.

Red panda amdoparvovirus (RPAV) was first described in captive red pandas (Ailurus fulgens) at a zoo in the United States in 2018. Subsequently, the prevalence of infection in zoos in the United States was reported to be 50%; however, RPAV prevalence outside the United States remains unstudied. This study was conducted to investigate the prevalence of RPAV in 134 red pandas from zoos in Europe. Overall, RPAV was detected with PCR in 21 of 62 zoos (33.9%), and the virus prevalence among individuals was estimated to be 24.2% (95% confidence interval, 17.4%-32.0%). Remarkably, adult females tested positive for RPAV more frequently than adult males. Zoos where RPAV was detected reported a significantly higher occurrence of alopecia (and clinical signs in general), whereas other commonly reported problems (fecal disorders and dental disease) showed no difference. A repeated pooled sampling of two positive individuals further showed that RPAV excretion in feces is intermittent, with the viral DNA being only detected on 8 out of 14 sampling days. The intermittent nature of excretion implies that RPAV prevalence may be higher than the estimated value.

Solid lipid nanoparticle formulation and antihyperglycemic activity test of sea cucumber (Stichopus hermanii)

This study aims to develop a formulation with the active ingredient Stichopus hermanii for antidiabetic treatment or function to lower blood glucose levels. The formulation approach taken is to design a formula in the form of solid lipid nanoparticles (SLN), where this system is expected to optimize absorption and improve the performance of the active substance. The SLN characterization includes particle size measurement, zeta potential, polydispersity index analysis, and morphology analysis using TEM. The antihyperglycemic activity test will be tested in vivo using male white rats using the glucose tolerance test method and induced by streptozotocin. The results of the TEM analysis showed that the sea cucumber spherical in shape. The particle size of the formula was found to be 0.25 nm. The polydispersion index (PDI) of the formulation was 67.5%, 2%, and 0.5%, respectively. In the same test, male rats were given treatment according to the group division, namely 50% glucose at a dose of 3 g/kg bw 30 minutes later and the blood sugar levels were measured at 30, 60, 90, and 120 minutes using a glucometer. In a follow-up test, the administration of test samples (days -3, 6, 9, 12, and 15) of CMC Na, metformin, and 25 mg/kg body weight of the test group showed that blood sugar level decreased with the highest decrease in positive control group. In conclusion, the results showed that sea cucumbers could be developed as a functional food product to help battle the onset of diabetes and diabetic complications.

How Organizational Cynicism Can Promote Customer-Directed Deviance Via Employee Resource Depletion And How Experiencing Supervisory Support May Help Overcome This Effect

While research suggests that organizational cynicism prompts employees to engage in organizationally directed deviant behaviors, questions remain regarding whether, how and when organizational cynicism can also prompt employees to engage in deviance toward customers, who are third parties outside the organization-employee exchange relationship. Drawing on conservation of resources theory, we develop and test a model that addresses these questions. We theorize that organizational cynicism is an adverse and stressful experience that drains employees’ psychological resources over time, which in turn leads to customer-directed deviance. We further propose that perceptions of supervisor support help employees cope functionally with their lack of resources and reduce customer-directed deviant behavior. We tested our predictions in a two-week daily experience sampling study of call-center representatives and found that organizational cynicism indirectly predicts customer-directed deviant behavior via resource depletion when PSS is lower than when it is higher. Two post hoc studies (including a three-wave panel survey conducted over 6 months and a three-wave, time-separated survey conducted over one month) addressed the methodological limitations of this investigation and ruled out alternative explanations for our results. Implications for organizational cynicism and customer service literature are discussed.

Identification of Lipids and Cytokines in Plasma and Follicular Fluid before and after Follicle-Stimulating Hormone Stimulation as Potential Markers for Follicular Maturation in Cattle.

The process of follicle maturation leading to ovulation is a key milestone in female fertility. It is known that circulating lipids and cytokines play a role in the follicle's ability to go through follicular maturation and the ovulatory processes. However, the specific mechanisms are not well understood. We posit that dysregulation of granulosa cells influences the ovarian environment, which tries to adapt by changing released lipids and cytokines to achieve follicular maturation. Eleven non-lactating adult females underwent estrus synchronization with two injections of PGF2α 14 days apart. Daily blood samples were collected for 28 days to monitor steroid hormone production after the second injection. To understand the potential impacts of lipids and cytokines during ovulation, a low-dose FSH stimulation (FSHLow) was performed after resynchronization of cows, and daily blood samples were collected for 14 days to monitor steroid hormone production until ovariectomies. The lipidomic analysis demonstrated increased circulating diacylglycerides and triacylglycerides during the mid-luteal phase and after FSHLow treatment. Cholesteryl esters decreased in circulation but increased in follicular fluid (FF) after FSHLow. Increased circulating concentrations of TNFα and reduced CXCL9 were observed in response to FSHLow. Therefore, specific circulating lipids and cytokines may serve as markers of normal follicle maturation.

In-Line Registered Milk Fat-to-Protein Ratio for the Assessment of Metabolic Status in Dairy Cows.

This study endeavors to ascertain alterations in the in-line registered milk fat-to-protein ratio as a potential indicator for evaluating the metabolic status of dairy cows. Over the study period, farm visits occurred biweekly on consistent days, during which milk composition (specifically fat and protein) was measured using a BROLIS HerdLine in-line milk analyzer (Brolis Sensor Technology, Vilnius, Lithuania). Clinical examinations were performed at the same time as the farm visits. Blood was drawn into anticoagulant-free evacuated tubes to measure the activities of GGT and AST and albumin concentrations. NEFA levels were assessed using a wet chemistry analyzer. Using the MediSense and FreeStyle Optium H systems, blood samples from the ear were used to measure the levels of BHBA and glucose in plasma. Daily blood samples were collected for BHBA concentration assessment. All samples were procured during the clinical evaluations. The cows were categorized into distinct groups: subclinical ketosis (SCK; n = 62), exhibiting elevated milk F/P ratios without concurrent clinical signs of other post-calving diseases; subclinical acidosis (SCA; n = 14), characterized by low F/P ratios (<1.2), severe diarrhea, and nondigestive food remnants in feces, while being free of other post-calving ailments; and a healthy group (H; n = 20), comprising cows with no clinical indications of illness and an average milk F/P ratio of 1.2. The milk fat-to-protein ratios were notably higher in SCK cows, averaging 1.66 (±0.29; p < 0.01), compared to SCA cows (0.93 ± 0.1; p < 0.01) and healthy cows (1.22). A 36% increase in milk fat-to-protein ratio was observed in SCK cows, while SCA cows displayed a 23.77% decrease. Significant differences emerged in AST activity, with SCA cows presenting a 26.66% elevation (p < 0.05) compared to healthy cows. Moreover, SCK cows exhibited a 40.38% higher NEFA concentration (p < 0.001). A positive correlation was identified between blood BHBA and NEFA levels (r = 0.321, p < 0.01), as well as a negative association between BHBA and glucose concentrations (r = -0.330, p < 0.01). Notably, AST displayed a robust positive correlation with GGT (r = 0.623, p < 0.01). In light of these findings, this study posits that milk fat-to-protein ratio comparisons could serve as a non-invasive indicator of metabolic health in cows. The connections between milk characteristics and blood biochemical markers of lipolysis and ketogenesis suggest that these markers can be used to check the metabolic status of dairy cows on a regular basis.

A new pharmacokinetic model of urinary methotrexate to assess adherence in rheumatoid arthritis

Background and objectivesMethotrexate (MTX) is the first-line therapy for rheumatoid arthritis (RA). While therapeutic adherence is essential to successful management, no objective MTX assay is currently available. Using population pharmacokinetic modelling (PopPK), our objective was to describe the urinary MTX (MTXu) kinetics in treated patients and to evaluate its abilities to assess the MTX-adherence. MethodsThe association between urinary methotrexate level and methotrexate administration was assessed using a generalized linear model. Then, a population pharmacokinetic model was developed based on data from 59 patients using with Monolix 2021. R2. Simulations were run to establish a reference kinetic profile and evaluate the proportion of samples predicted as true positives. ResultsCompared to the control group, multivariate analysis showed that MTXu was independently associated with methotrexate administration (p < 0.0001) with a sensitivity and specificity greater than 99%. The final PopPK model selected was a two-compartment model with first-order absorption and elimination. Internal and external validation of the model met all predefined criteria. When using an analytical assay with a LOQ equal to 1 nM, the proportion of samples predicted as true positives is over 90%, as a function of MTX dose (7.5–25 mg/week) and post-administration sampling days (1–7 days). ConclusionWe developed a pharmacokinetic model able to describe expected patterns of urinary methotrexate. This allowed us to propose a new objective test of MTX adherence, which could help in routine practice to differentiate patients who are truly unresponsive to methotrexate from those who are unresponsive because of non-adherence.

Baseline soil-transmitted helminth and schistosome infection in the Geshiyaro project, Ethiopia: A unique transmission interruption project using biometric fingerprinting for longitudinal individual analysis.

The Geshiyaro project aims to assess the feasibility of interrupting transmission of soil-transmitted helminths (STH) and schistosome (SCH) infection in the Wolaita zone of southern Ethiopia through high coverage community-wide mass drug administration (MDA), in combination with improved water, sanitation, and hygiene services and behaviour change communication delivered through the existing health care infrastructure. To accurately measure treatment coverage a population census was conducted enrolling individuals with biometric fingerprinting and barcoded ID cards. This paper details the baseline census and parasitology surveys conducted before the start of any interventions. The census was conducted in five of the 15 Wolaita districts between October 2018 and December 2019, enrolling all consenting participants from every household. Simultaneously, a cross-sectional parasitology survey was conducted in 130 out of 361 randomly selected communities from all 15 districts, with 100 individuals across all age groups (infant to adult) per community providing stool and urine for analysis by duplicate Kato-Katz and a point-of-care circulating cathodic antigen (POC-CCA) to test for Schistosoma mansoni and STH, and microhaematuria and urine filtration for Schistosoma haematobium. Of the 130 communities, 30 were randomly selected for annual, longitudinal parasitological monitoring, with 150 randomly selected individuals from infant to adult providing two days of stool and urine samples for analysis by the same diagnostic tests per community. In total 97,919 households participated in the baseline census enrolling 466,071 individuals, with parasitological data obtained from 10,785 people. At baseline, 15.5% were infected with at least one STH species, with Ascaris lumbricoides (9.5%), followed by hookworm (7.2%) and Trichuris trichiura (1.8%). Substantial heterogeneity in STH prevalence was observed between communities ranging from 0% to 61% where most infections were low intensity. Schistosoma mansoni infection was the dominant schistosome infection (0.85% by Kato-Katz and 13.3% by POC-CCA trace negative and 21.5% trace positive), with few Schistosoma haematobium infections identified (2.77% haematuria positive and 0.13% positive by urine filtration). While the national control program in Ethiopia has made good progress in reducing prevalence of STH and SCH in Wolaita since it was launched in 2015, there remain areas of persistent infection suggesting the existence of environmental or behavioural risk factors that contribute to ongoing transmission. This project aims to identify the most efficient intervention strategies to reduce community burden and reach interruption of transmission.

Noninvasive Glucose Sensing in Dielectrically Equivalent Multilayer Skin Phantoms.

The interstitial fluid of the skin contains glucose levels comparable to those of blood. Noninvasive glucose sensing by microwaves has great potential to relieve diabetics from the burden of daily blood sampling, but improving the selectivity of this method remains a challenge. This study reports a dielectrically equivalent multilayer skin phantom and provides insight into the criteria for noninvasive glucose sensing by conducting dielectric analysis. The skin phantom was a hydrogel composed of gelatin, glucose, sodium chloride, and water covered by paraffin-impregnated paper. Investigations conducted on a wide range of component concentrations revealed characteristic relative permittivity and dielectric loss determined by the amount of electrolyte and solution that was independent of the amount of glucose. Since the microwave response due to glucose tends to be buried in noise, we developed a flowchart that first identifies the amounts of electrolytes and proteins, which are the major components other than glucose, and then quantifies the remaining glucose content. This noninvasive glucose sensing method would not be limited to the medical healthcare field; it could potentially be used in food manufacturing processes, livestock farming, and plant cultivation management.

A large-scale experiment finds no consistent evidence of change in mortality or commercial productivity in silverlip pearl oysters (Pinctada maxima) exposed to a seismic source survey

High-intensity, impulsive sounds are used to locate oil and gas reserves during seismic exploration of the seafloor. The impacts of this noise pollution on the health and mortality of marine invertebrates are not well known, including the silverlip pearl oyster (Pinctada maxima), which comprises one of the world's last remaining significant wildstock pearl oyster fisheries, in northwestern Australia. We exposed ≈11,000 P. maxima to a four-day experimental seismic survey, plus one vessel-control day. After exposure, survival rates were monitored throughout a full two-year production cycle, and the number and quality of pearls produced at harvest were assessed. Oysters from two groups, on one sampling day, exhibited reduced survival and pearl productivity compared to controls, but 14 other groups receiving similar or higher exposure levels did not. We therefore found no conclusive evidence of an impact of the seismic source survey on oyster mortality or pearl production.

Effect of a Chitosan Coating Enriched with an Olive Leaf Extract on the Characteristics of Pork Burgers.

Chitosan coatings have been investigated for improving food shelf-life. The addition of an olive leaf extract could enhance its beneficial effect. The aim of this study was to evaluate the effectiveness of an olive leaf extract added to a chitosan coating in delaying deterioration in refrigerated pork burgers without additives packaged under a 40% oxygen and 60% carbon dioxide modified atmosphere. Some general parameters (microbial counts, instrumental color and texture, and lipid and protein oxidation) were measured over the storage of pork burgers without coating (Control), with a chitosan-based coating (Chitosan) and with a chitosan-based coating enriched with an olive leaf extract (Chitoex). The coating impacted the effect of the storage time on most parameters. Both coatings were especially effective at limiting the changes that occur over time in the headspace gases, some texture parameters (hardness, gumminess, and chewiness) and lipid oxidation, although the effect on the microbial counts was weak. Chitoex was more effective than Chitosan at preventing changes in the headspace gases on day 11 and in lipid oxidation on all the sampling days. In conclusion, the Chitoex coating could be useful for prolonging the storage of pork burgers by preventing changes in texture and reducing lipid oxidation.

Vertical distribution of microplastics in a river water column using an innovative sampling method.

Due to limitations of sampling methods, subsurface water is usually a less well-investigated compartment of the water column when scientists assess microplastic contamination. In this study, microplastic (MP) contamination was assessed in a freshwater river both in surface and subsurface using an innovative sampling method. Microplastic contamination in the lower part of the water column, i.e., near-bottom water and in sediments, was also studied. Three sampling campaigns were carried out during different weather conditions: stormy, rainy, and dry in order to observe their influence on the microplastics vertical distribution. No significant difference was observed between the abundance and types of MPs in surface and subsurface water. The proportion of polymer with theoretical density < 1 (polypropylene d = 0.9, polyethylene d = 0.91-0.95) and polystyrene (d = 0.1-1.06) in the surface and subsurface samples was 73.5%, and this proportion drops to 40.8% for the samples located in the near-bottom water and the sediments. Our results indicate that the MP concentration of the different compartments analyzed can be significantly influenced by rainfall during and prior to the sampling day. This study highlights that in shallow rivers, surface water sampling is representative of the water column MP contamination, but that sampling without taking environmental conditions into account may lead to erroneous estimation of MPs concentration and flux entering the marine environment.

Underestimated climate change impacts on hydro-wind-photovoltaic complementary systems due to neglect of short-term features

Hydro-wind-photovoltaic (PV) complementary power systems (HWPCSs) offer a promising solution for integrating intermittent wind and PV power. It is crucial to consider short-term features (e.g., variations in the hydropower efficiency and intraday curtailment characteristics) in the long-term HWPCS operation for achieving more accurate simulations. However, current long-term evaluations of HWPCSs under climate change normally neglect variations in short-term features that are difficult to be extracted, leading to potential misestimations of climate change impacts. This study proposes a generic framework to quantify this misestimation by extracting short-term features of HWPCSs from simulated daily operating samples. The short-term features are then incorporated into a long-term model to simulate operating results under climate change. Results of a case study in the Yalong River Basin validate that considering short-term features effectively increases the accuracy of calculating long-term HWPCS power generation. With the misestimation of both hydropower output and power curtailment, neglecting short-term features causes over-optimistic evaluations of future HWPCS performances. Specifically, the future HWPCS power generation and guaranteed rate are overestimated by an average of 1.02% and 1.09%, respectively, whereas the future power curtailment rate is underestimated by an average of 4.01%. The proposed framework contributes valuable insights for the long-term operation and evaluation of HWPCSs under climate change.

Artisanal fisheries catch highlights hotspot for threatened sharks and rays in the Republic of the Congo

AbstractGlobal catch rates of sharks and rays from artisanal fisheries are underreported, leading to a lack of data on population status. This forms a major barrier to developing effective management plans, such is the case in Central and West Africa. Over 3 years, we undertook the first systematic quantitative assessment of sharks and rays landed by an artisanal fishery in the Republic of the Congo. During 507 sampling days (mean 14 surveys per month), we recorded 73,268 individuals. These comprised 42 species, of which 81% are considered at an elevated risk of extinction. Landings were dominated by immature individuals, especially for species of conservation concern. Presence of species thought to have largely disappeared from the region such as the African wedgefish (Rhynchobatus luebberti) and smoothback angelshark (Squatina oculata) suggest Congolese waters are a potential stronghold for these species—warranting increased protection. We identified seasonality of catch within years, but not across years. Both inter‐ and intra‐annual trends varied by species, signifying annual fluctuations in catch of each species but consistent catch of all species year‐on‐year. Analysis showed increased catch between the short‐wet and the long‐wet, and the long‐dry seasons (January–February and August–September). Lowest catch was shown to occur during the short‐wet and the short‐dry seasons (October–December), which may provide an opportunity for seasonal closures or gear restrictions.

Haematological alterations under the anti-helminthic application in Pavo cristatus

ABSTRACT Anti-helminthic drugs (Albendazole and Levamisole) trials were conducted on Indian Peafowls (n = 20) kept at Jallo Wildlife Park, Lahore, Pakistan for 15. Sampling was conducted on days 7 and 15 of treatments. The results showed that the WBC count was significantly (P < 0.05) higher in both samples in response to Levamisole treatment. Haemoglobin (HGB) and Mean Corpuscular Haemoglobin Concentration (MCHC) concentration increased significantly (P < 0.05) in response to both treatments; however, Mean Corpuscular Haemoglobin (MCH) decreased significantly (P < 0.05) in Albendazole-treated birds on both the sampling days compared to the control. Red Blood Cell Distribution Width-Standard Deviation (RDW-SD) decreased significantly (P < 0.05) in all treatment groups. On the other hand, the prevalence of eggs per gram (EPG) was reduced by 20% after the application of Levamisole on days 7 and 15. It was concluded that both drugs had significant effects on WBCs, HGB, MCH, MCHC and RDW-SD.

Associations between light exposure and sleep timing and sleepiness while awake in a sample of UK adults in everyday life

Experimental and interventional studies show that light can regulate sleep timing and sleepiness while awake by setting the phase of circadian rhythms and supporting alertness. The extent to which differences in light exposure explain variations in sleep and sleepiness within and between individuals in everyday life remains less clear. Here, we establish a method to address this deficit, incorporating an open-source wearable wrist-worn light logger (SpectraWear) and smartphone-based online data collection. We use it to simultaneously record longitudinal light exposure (in melanopic equivalent daylight illuminance), sleep timing, and subjective alertness over seven days in a convenience sample of 59 UK adults without externally imposed circadian challenge (e.g., shift work or jetlag). Participants reliably had strong daily rhythms in light exposure but frequently were exposed to less light during the daytime and more light in pre-bedtime and sleep episodes than recommended [T. M. Brown et al., PLoS Biol. 20, e3001571 (2022)]. Prior light exposure over several hours was associated with lower subjective sleepiness with, in particular, brighter light in the late sleep episode and after wake linked to reduced early morning sleepiness (sleep inertia). Higher pre-bedtime light exposure was associated with longer sleep onset latency. Early sleep timing was correlated with more reproducible and robust daily patterns of light exposure and higher daytime/lower night-time light exposure. Our study establishes a method for collecting longitudinal sleep and health/performance data in everyday life and provides evidence of associations between light exposure and important determinants of sleep health and performance.

Analisis Sifat Fisis dan Mekanik Biodegradable Foam Berbahan Dasar Selulosa Jerami Padi dan Polivinyl Alcohol

Research has been carried out on analyzing the physical and mechanical properties of biodegradable foam made from rice straw cellulose with a polyvinyl alcohol binding matrix. This study aims to produce a substitute for styrofoam, which is considered not environmentally friendly and harmful to health. The composition of straw fiber and polyvinyl alcohol was varied by the mass ratio of the mixture of rice straw fiber and PVA (%) 40:20%, 30:30%, 20:40%, 50:10%, with the size of the fiber passing through the 80 mesh sieve. The results of the characterization using FT-IR showed that the highest content of functional groups was carbon (C), which corresponds to the content of conventional biofoam, which consists of the elements carbon (C) and hydrogen (H). The highest biofoam tensile strength value is in the comparison fraction (20:40%) of 18.11 MPa. The highest percentage of biofoam water absorption was obtained in the fraction ratio (50:10%) of 0.8928% in the treated sample and 1.1928% in the untreated sample. The percentage of biofoam water content closest to conventional biofoam water content is obtained at a fraction ratio (40:20%) of 0.78%. The highest degradation rate was obtained in samples with a comparative fraction (50:10%) of 0.04237%/day in treated and 0.06403%/day in untreated samples. The results of this study stated that the resulting biofoam did not contain harmful substances. After identifying the functional groups using the FT-IR test, the tensile strength of biofoam still did not meet the SNI 7188.7: 2016 bioplastic standard.

Characteristics and Health Risk Assessment of Volatile Organic Compounds in Different Functional Zones in Baoji in Summer

The situation of air pollution in Guanzhong Plain has been increasing in recent years; hence, it is very important to study the characteristics of volatile organic compounds (VOCs) and their health risks in urban functional zones. We analyzed 115 VOCs using gas chromatography-mass spectrometry/hydrogen ion flame detector (GC-MS/FID) and high performance liquid chromatography (HPLC) at four sampling sites in the traffic, comprehensive, industrial, and scenic zones of Baoji. We analyzed the main components and key species in the different functional zones. Ozone formation potential (OFP),·OH consumption rate (L·OH), and secondary organic aerosol formation potential (SOAFP) were used to evaluate the environmental impact, and the hazard index (HI) and lifetime cancer risk (LCR) methods were employed. The results revealed that the mean values of φ(TVOCs) in the traffic, comprehensive, industrial, and scenic zones were (59.63±23.85)×10-9, (42.92±11.88)×10-9, (60.27±24.09)×10-9, and (55.54±7.44)×10-9, respectively. The dominant contributors at the traffic zone were alkanes, and those at the other functional zones were OVOCs. Acetaldehyde, acetone, n-butane, and isopentane were abundant at different functional zones. According to the characteristic ratios of VOCs, the average ratio of toluene to benzene (T/B) at the traffic, comprehensive, industrial, and scenic zones were 1.84, 2.39, 1.28, and 1.64, respectively, and the ratio of iso-pentane to n-pentane (i/n) was mainly between 1 and 4. The results indicated that VOCs in Baoji were significantly affected by vehicle emissions and gasoline evaporation, biomass and coal combustion, and industrial coatings and foundry. The ratio of m/p-xylene to ethylbenzene (X/E) was lower than 2 at the four functional zones, and the minimum was 1.79 at the scenic zones; the results revealed that X/E was small, and the aging degree of air masses was high, indicating the influence of regional transport. According to the ratio of formaldehyde to acetaldehyde (C1/C2) and the ratio of acetaldehyde to propanal (C2/C3), it was suggested that there may have been evident anthropogenic emission sources, and the photochemical reaction had an important effect on aldehydes and ketones. Environmental impact assessment results revealed that OVOCs and alkenes contributed significantly to OFP and OFP from large to small was as follows:industrial zone>scenic zone>traffic zone>comprehensive zone. The range of L·OH in each functional zone was 8.77-15.82 s-1, with isoprene contributing the most in the industrial zone and acetaldehyde contributing the most at other functional zones. The SOAFP of each functional zone was as follows:scenic zone>comprehensive zone>traffic zone>industrial zone. Toluene, m/p-xylene, and isoprene were the notable species. According to the health risk assessment of EPA, the HI of toxic VOCs in all functional zones was lower than 1, which was at an acceptable level. However, the number of days with HI>1 in industrial zones accounted for 42.86% of the total sampling days, indicating a high risk. The lifetime carcinogenic risk (LCR) of the traffic, comprehensive, industrial, and scenic zones were 1.83×10-5, 1.21×10-5, 1.85×10-5, and 1.63×10-5, respectively, which were all in grade Ⅲ of the rating system, indicating a high probability of cancer risk. Species with LCR greater than 10-6 were formaldehyde; acetaldehyde; 1,2-dibromoethane; 1,2-dichloroethane; 1,2-dichloropropane; and chloroform.

Time from feeding impacts farmed Atlantic salmon (Salmo salar) gut microbiota and faecal score

In intensive Atlantic salmon aquaculture, faecal score potentially serves as a generalised indicator of gut microbiome diversity and composition and as a proxy for gut transit time. There is also the possible application to detect dysbiosis. The utility of using faecal scoring for assessing commercially farmed fish populations may, however, be impacted by rapid temporal changes in digestive status following feeding that, as yet, has not been considered. To help inform sampling strategies and better understand the utility of faecal scoring for farmed fish, this study documented Atlantic salmon faecal scores and gut bacterial 16S rRNA amplicon sequence profiles over 24 h following a feeding event. Samples were collected from two marine cohorts of salmon in different seasons (summer and winter), and comparisons were also made between non-destructive stripped hindgut faeces (HS) and destructive sampling of hindgut digesta (HD) and hind gut mucosa (HM). Average hindgut faecal scores and microbiome diversity were largely consistent over a normal (6 to 8 h) sampling day in winter, but were much more variable in summer, potentially associated with faster faecal passage and dynamic gut microbial changes in the hours after feeding. HS samples were determined to be a valid non-destructive method to obtain material for assessing the diversity and composition of transient bacterial communities in the distal intestine. For an accurate representation of both the transient and resident bacterial communities, both sample types should be considered. It is recommended that studies comparing treatment cohorts, or between individuals in large sample sets, should be designed to capture temporal and seasonal effects that are associated with changes in digestive status and gut microbiome.

Risk assessment for biopharmaceutical single-use manufacturing: A case study of upstream continuous processing

In the current transition to intensified upstream processing, the risks of adopting traditional single-use systems for high-titer, long-duration perfusion cultures, have thus far not been considered. This case study uses the Failure Modes and Effects Analysis (FMEA) method to evaluate the risks associated with implementing upstream single-use technology. The simulated model process was used to compare the risk level of single-use technology for a traditional fed-batch cell culture with that for perfusion culture, under the same annual protein production conditions. To provide a reasonable source of potential risk for FMEA, all single-use upstream operations for both fed-batch and perfusion processes were investigated using an analytical method developed to quantify the impact of process parameters and operating conditions on single-use system specifications and to ensure objectivity. Many of the risks and their levels, were similar in long-duration perfusion cultures and fed-batch cultures. However, differences were observed for high-risk components such as daily sampling and installation. The result of this analysis indicates that the reasons for risk are different for fed-batch cultures and perfusion cultures such as larger bioreactors in fed-batch and longer runs in perfusion, respectively. This risk assessment method could identify additional control measures and be part of a holistic contamination control strategy and help visualize their effectiveness.