Pigs cannulated at the terminal ileum were used to investigate the effect of weaning age on specific intestinal microflora and fermentation acids. In two replicate trials performed 50 days apart, a total of twenty-four 14-day-old nursing pigs from 6 sows were cannulated in the terminal ileum and randomly assigned to either a 21-day (W3) or 28-day (W4) weaning interval. Ileal samples were collected at 20, 23, 27, 30, 34, 37, and 41 days of age. Samples were assayed for total lactobacilli, total and hemolytic coliforms, streptococci, pH, and dry matter. Concentrations of coliforms increased (P < .05) postweaning in both W3 and W4 pigs. Lactobacilli concentrations decreased (P < .05) nearly 1000-fold postweaning in W3 pigs while concentrations decreased (P < .05) 30-fold in W4 pigs postweaning. Percentage of hemolytic coliforms increased (P < .05) with age of pigs on both treatments and continued to increase in W3 pigs through the end of the study. Streptococci concentrations were not affected by treatment. Ileal pH increased (P < .05) postweaning in W3 pigs but remained constant in W4 pigs. Dry matter decreased (P < .05) in both groups postweaning, but lower values were more persistent in W3 pigs. Total volatile fatty acid concentrations decreased (P < .05) in both groups following their respective weaning dates, while total lactic acid concentrations increased (P < .05) in both groups following weaning. Temporary shifts in ileal microflora populations, pH, and dry matter were of greater magnitude and persisted for longer periods in pigs weaned at 21 days compared with pigs weaned at 28 days.
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