The Hotel Tree of Life is situated in a 26 ha land area at Barigama (7°31‟84”N 80°57‟74”E)in Kandy district, central Province of Sri Lanka. This hotel was established in an old teaestate premises that was present about 100 years ago. During the last 100 years, uponabandoned, the tea estate had overgrown into a private owned secondary forest. The area nowconsists of a 25 ha forest that is owned and managed by the hotel.The study was conducted from August 2014 to July 2015 in the hotel and the adjacent forestarea. Visual encounter (assisted by the calls) sampling method was used during day and nightsampling. Four species from the Critically Endangered (CR) Red Data category were againpatch sampled for micro-habitat, by using quadrat (1×1 m2). Minimal disturbance to habitatwas considered a priority at all sampling locations. Different micro-habitats were sampled toobtain an overall diversity reading to find out the observable diversity of amphibians in thehotel. All sampled species were handled, using capture and released at the same location.After having the quadrate sampling, it was found that one location had a diverse populationof Pseudophilautus zorro known to be CR. One of the rare findings was the Pseudophilautuscf semiruber that was found in the hotel executive staff quarters. The species wasrediscovered after 99 years in 2012 and known to be Data Deficient (DD).19 species of amphibians from six families were found during the study (one speciesLankanectus corrugatus is an endemic genus to Sri Lanka). From those identified, 14 species(74%) are endemic and five are non-endemic (26%). Family Bufonidae (Duttaphrynusmelanostictus), Ranidae (Hylarana gracilis), Dicroglossidae (Ramanella obscura),Microhylidae (Zakerana keertisinghei, Z. syhadrensis, Hoplobatrachus crassus, Euphlyctiscyanophlyctis, E. hexadactylus), Nyctibatrachidae (Lankanectes corrugatus), Rhacophoridae(Polypedates cruciger, Pseudophilautus pleurotaenia, P. fergusonianus, P. cavirostris, P.zorro, P. semiruber, P. rus, P. popularis, P. hallidayi, P. schneideri) were found during thestudy. By considering these findings, the hotel can be considered as a potential amphibianhotspot. A general habitat enrichment program and awareness notices (biodiversity protectedarea) were placed in the area as a conservation measurement. Numerous waterholes werecreated in the location with up-cycled material, to enhance habitat diversity.Keywords: Diversity, Amphibia, Anura, Conservation, Critically Endangered, Biodiversityprotected area, Pseudophilautus