PurposeFor 13C echo‐planar imaging (EPI) with spectral‐spatial excitation, main field inhomogeneity can result in reduced flip angle and spatial artifacts. A hybrid time‐resolved pulse sequence, multi‐echo spectral‐spatial EPI, is proposed combining broader spectral‐spatial passbands for greater off‐resonance tolerance with a multi‐echo acquisition to separate signals from potentially co‐excited resonances.MethodsThe performance of the imaging sequence and the reconstruction pipeline were evaluated for 1H imaging using a series of increasingly dilute 1,4‐dioxane solutions and for 13C imaging using an ethylene glycol phantom. Hyperpolarized [1‐13C]pyruvate was administered to two healthy rats. Multi‐echo data of the rat kidneys were acquired to test realistic cases of off‐resonance.ResultsAnalysis of separated images of water and 1,4‐dioxane following multi‐echo signal decomposition showed water‐to‐dioxane 1H signal ratios that were in agreement with the independent measurements by 1H spectroscopy for all four concentrations of 1,4‐dioxane. The 13C signal ratio of two co‐excited resonances of ethylene glycol was accurately recovered after correction for the spectral profile of the redesigned spectral‐spatial pulse. In vivo, successful separation of lactate and pyruvate‐hydrate signals was achieved for all except the early time points during which signal variations exceeded the temporal resolution of the multi‐echo acquisition.ConclusionImproved tolerance to off‐resonance in the new 13C data acquisition pipeline was demonstrated in vitro and in vivo. Magn Reson Med 80:925–934, 2018. © 2018 International Society for Magnetic Resonance in Medicine.
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