Abstract The Serayu River is a national strategic river that provides essential water to local water companies, industrial operations, agriculture, and household irrigation in the surrounding community. The Serayu River’s condition is currently deteriorating due to erosion and pollution. The primary goal of this investigation is to quantify the Serayu River’s water pollution. Data from continuous online monitoring stations at Wanganaji Dam (upstream) and Gerak Serayu Dam (downstream) will be used to assess temperature, DO, pH, TDS, DHL), turbidity, and ammonia levels. The research methodology for this study included descriptive statistical analysis and the STORET method, which is based on the regulatory framework established in the Republic of Indonesia by Ministerial Decree Number 115 of 2003 and Government Regulation Number 22 of 2021. The results show that the water quality upstream of the Serayu River (Wanganaji Dam Station) partially meets most of the measured parameters while still meeting the required quality standards. Meanwhile, the water quality in the downstream part of the Serayu River (Gerak Serayu Dam Station) has DO values (3.68-4.98 mg/L), turbidity (36.78-95.48 mg/L) that exceed the required quality standards. The water quality at the Serayu River’s two dams ranges from moderate to heavily polluted.
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