Dairy Sector Research Articles

On-farm investments into dairy cow health: evidence from 15 case study countries.

Managing investments in dairy cow health at a national and global scale, requires an improved understanding of current on-farm expenses for cow health (e.g., expenditure for medicine and veterinary consultations). The aim of this study was to assess on-farm health investments for typical dairy farms in 15 case study countries, including Argentina, Australia, Bangladesh, Brazil, Canada, India, China, Colombia, Indonesia, Kenya, New Zealand, Uganda, UK, Uruguay, and USA. The study was conducted using a descriptive analysis of a secondary data set that was obtained from the International Farm Comparison Network (IFCN). The results suggest that health expenditures take up a relatively small proportion (<10%) of the annual total production costs per cow across all countries in the sample. The means of production costs (e.g., feed, machinery) can take up to 90% of the total production costs for highly intensive systems, while these costs can be as low as 9% for extensive systems. This study highlights the importance of understanding on-farm animal health investments as a contribution to improved national and global decision making about animal health in the dairy sector.

Prediction, Synthesis and Evaluation of a Synthetic Peptide as an Enzyme-Linked Immunosorbent Assay (ELISA) Candidate for Screening of Bovine Antibodies against Theileria annulata.

Tick-borne diseases (TBDs) of livestock are endemic across various parts of tropical countries. Theileriosis is one such economically important TBD, caused by the Theileriidae family of organisms, which is transmitted by ticks. Theileria annulata, the causative agent of tropical theileriosis, contributes a significant loss to the dairy sector by causing anorexia, high fever, anemia, inflammatory changes in vital organs and icterus, thus, a loss in milk yield. Though vaccines are available, their protective efficacy is not absolute, and treatment is limited to early diagnosis of the causative agent. Routinely, microscopic identification of piroplasms in the erythrocytes (Giemsa-stained) of infected animals or schizonts in lymph node biopsies are practiced for diagnosis. PCR-based techniques (multiplex, uniplex, nested and real-time) have been reported to perform well in diagnosing active infection. Several attempts have been made using serological assays like Dot blot, ELISA and ICT, but the results were of variable sensitivity and specificity. Recombinant proteins like the Theileria annulata merozoite surface antigen (Tams1) and Theileria annulata surface protein (TaSP) have been explored as antigenic candidates for these assays. In the present study, we predicted an immunogenic peptide, i.e., TaSP-34, from the TaSP using various computational tools. The predicted peptide was custom synthesized. The diagnostic potential of the peptide was assessed by indirect plate ELISA to detect the bovine-IgM against Theileria annulata. Alongside, a recombinant truncated TaSP (rTaSP(tr)) was expressed and purified, which was used to compare the performance of the peptide as a diagnostic candidate. The IgM-based peptide ELISA was 100% sensitive and 92.77% specific as compared to PCR (Tams1 targeting), while 98.04% sensitivity and 97.44% specificity were observed in comparison with rTaSP(tr) ELISA. Almost perfect agreement between peptide ELISA and Tams1 PCR was observed with a Cohen's kappa coefficient (κ-value) of 0.901 and agreement of 95.31%. Further, the κ-value between the peptide ELISA and rTaSP(tr) ELISA was found to be 0.95, and the agreement was 97.65%, which shows a good correlation between the two tests. The findings suggest that the TaSP-34 peptide can be an efficient and new-generation diagnostic candidate for the diagnosis of T. annulata. Furthermore, the peptide can be synthesized commercially at a larger scale and can be a cost-effective alternative for the protein-based diagnostic candidates for T. annulata.

92 Methane Mitigation in Dairy Animals Begins Before Birth with Opportunities to Impact Throughout Life

Abstract Research efforts are underway to identify methane mitigators in the dairy sector to reach net zero carbon emissions, and ideally, net negative global emissions. Methane mitigation is multifactorial, including strategies in genetics, management, diet composition, diet nutrient composition, and implementation of feed additives. Research efforts thus far have primarily focused on adult ruminants. Research on how calf management and its impact on phenotypic traits including milk production and methane production is currently lacking. Supplying different levels of nutrients in late lactation results in demonstrated splits in feed intake, average daily gain, metabolism, and greenhouse gas emissions from their offspring. Post-calving, nutrient-restricted heifers entering the lactating herd are hypothesized to be less productive and have greater environmental impact due to reduced productive efficiency relative to their control herd mates. Identification of feed additives that are locally sourced, alter the rumen microbiome, and have lifetime reductions on emissions are under investigation. Further research efforts include identifying metabolic and genetic profiles to identify animals in early life as either high or low methane emitters. Being able to classify animals early in life can reduce the total economic inputs used to raise animals and reduce the total environmental impact of these animals through early culling or intervention of high methane emitting animals. The goal of this presentation is to discuss current knowledge and future potential of calf management, feeding regimes, and feed additive implementation to reduce methane emissions and total environmental impact in the dairy industry.

Assessment of antibiotic use patterns in bovine mastitis treatment in the dairy sector in Serbia.

The dairy sector is essential for the world's food systems, playing a vital role in agricultural production. Yet, mastitis, an inflammation of the mammary gland, affects a considerable portion of the dairy cow population annually, leading to reduced productivity and sustainability. Insight into therapeutic protocols is essential for a better understanding of the situation on farms with the aim of developing new or harmonizing existing protocols in the mastitis treatment. This study conducted on dairy farm in Serbia aimed to explore the most commonly used antibiotics during bovine mastitis treatment and their association with daily milk yield. Data from 100 lactating cows, including therapeutic protocols, lactation numbers, mastitis episodes, milk yield, and antibiotic usage, were obtained from a computerized database and statistically analysed. The results demonstrated a high prevalence of narrow-spectrum antibiotics usage, with the majority of cows treated with combination therapy. The route of antibiotic application did not significantly impact daily milk yield, while treatment duration had no discernible effect. Nonetheless, specific antibiotics were associated with milk yield variations, suggesting the need for careful antibiotic selection and management in mastitis therapy. These findings emphasize the importance of prudent antibiotic use to safeguard animal health and long-term milk production sustainability.

Short-milking-tube thermograms: An alternative to udder thermograms for mastitis detection in Sahiwal cows

Mastitis is a multi-etiological production disease that causes substantial financial loss to dairy farmers. In this context, early detection of mastitis using thermograms can aid the dairy sector in managing mastitis efficiently, and this technology could be a supportive tool in precision dairy farming. Infrared cameras can detect minor temperature changes on the udder surface by taking multiple images of the udder and teat. In the current study, a thermogram of the short milking tube (SMT) of the milking machine, as well as the udder and teat of lactating Sahiwal cow (n = 100 quarters of 25 Sahiwal cows), was captured using a hand-held digital infrared thermal camera (DarviDTL007) during morning milking to assess the mastitis status. CMT and SCC of milk samples were carried out for further confirmatory diagnosis of healthy, sub-clinical (SCM), and clinical mastitis (CM). Cut-offs for short milking tube temperature were developed using the receiver operating characteristics analysis. Results of thermal image analysis revealed that the pre-milking, milking, and post-milking parameters of the udder and the teat skin surface temperatures showed a significant difference in the healthy, SCM, and CM-affected quarters. The thermogram analysis showed a significant increase (p < 0.05) of 1.11 and 2.04°C in the mean values of SMT surface temperature among SCM and CM quarters compared to healthy quarters, respectively. In addition, the values of CMT and SCC revealed a significant increase (p < 0.05) in SCM and CM samples and a positive correlation to SMT surface temperatures. Short milking tube thermograms can be a useful assessment tool for detecting sub-clinical mastitis in dairy animals.

What can we learn from the past? Tracking sustainability indicators for the Swedish dairy sector over 30 years

CONTEXTThe dairy sector has undergone profound transformation over recent decades, resulting in considerably fewer but larger and more specialised farms, with unclear implications across sustainability dimensions. OBJECTIVEThe objective was to develop and employ a framework for assessing sustainability in the Swedish dairy sector to shed light on how recent historical developments (1990–2020) have influenced sustainability outcomes. METHODSUsing a data-driven, multidisciplinary approach, main areas of concern for sustainability in the primary production stages of the dairy sector were identified. These were then populated with indicators to track developments over time and highlight synergies and trade-offs. RESULTS AND CONCLUSIONSFour areas of concern were identified and populated with eight indicators (listed in brackets): ‘supporting ecosystems’ (semi-natural grassland area, ley area, mean field size), ‘climate impact’ (methane from enteric fermentation), ‘animal welfare’ (veterinary treatments, percentage of culled cows due to diseases) and ‘farm viability’ (competitive wages, farmer age structure). The results showed that area of semi-natural grassland per dairy cow decreased by 27% from 2003 to 2020. Area of ley per cow decreased slightly but the proportion of arable land on dairy farms devoted to ley cultivation increased, due to improved roughage quality enabling an increase in proportion of roughage in feed rations. In terms of climate impact, enteric methane emissions per kg milk decreased by 21%. Regarding animal welfare, veterinary treatments of diseases decreased from 45% to 21% over the 30 years, with declining trends for most recorded diseases except hoof disease. The indicators for farm viability showed that the average dairy farm was unable to pay a wage comparable to the national average throughout most of the period 2004–2020, but a slightly positive trend was observed, although with large year-on-year variability. A rapid change in age structure was seen between 2003 and 2020, with the proportion of land managed by older farmers (+60 years) increasing from 12% to 22%, indicating challenges with demographic viability. SIGNIFICANCETracking changes over time across sustainability dimensions gives important insights into improvements made and challenges that remain to be solved. Overall, developments in the Swedish dairy sector have diminished its capacity to support ecosystems, particularly related to semi-natural grasslands, while reducing its climate impacts and improving animal welfare. An increased specialisation has also resulted in spillover effects where services and impacts have shifted from dairy herds to specialised beef herds. These findings are important in navigating policy processes targeting developments in the dairy sector.

In Silico Exploration and Experimental Validation of Camellia sinensis Extract against Rhipicephalus microplus and Sarcoptes scabiei: An Integrated Approach.

Sarcoptes scabiei is an ectoparasite of humans and animals that causes scabies. The Rhipicephalus (Boophilus) microplus is a blood-sucking ectoparasite that transmits various pathogens. These two parasites have caused great losses to a country's dairy and agriculture sectors. The aim of this study was to determine the in vitro and in silico efficacy of Camellia sinensis plant extracts. Different concentrations of C. sinensis ethanolic plant extracts were prepared using the maceration method and were used against mites and ticks (in adult immersion test AIT and larval packet test LPT) to evaluate their in vitro acaricidal activity. Additionally, in silico molecular docking was performed to investigate the inhibitory interactions between the phytochemicals of the plant and S. scabiei and R. microplus glutathione transferase proteins (SsGST and RmGST). This study observed that the plant extract showed high efficacy in vitro against mites and different tick stages in adult immersion and larval packet tests. Additionally, the in silico study revealed a strong binding interaction between ellagic acid and SsGST protein, with a binding energy of -7.3 kcal/mol, with respect to permethrin (-6.7 kcal/mol), whereas quercetin and RmGST resulted in a docking score of -8.6 kcal/mol compared to deltamethrin (-8.2 kcal/mol). Overall, this study explored the potential of C. sinensis as a natural alternative for controlling tick and mite infestations and provided insights into the inhibitory mechanisms of its phytochemicals.

Prevalence and Risk Factors of Bovine Mastitis on Conventional Dairy Farms in Northwestern Algeria

Background: Algeria’s dairy sector is experiencing various obstacles, such as a high frequency of diseases in dairy farms, particularly mastitis. Nonetheless, there has been a scarcity of research on mastitis occurrence, notably in Algeria’s Western area. As a result, the purpose of this study was to investigate the prevalence of mastitis and its associated risk factors in Algeria’s northwest area. Methods: The study included 130 dairy cows from 17 farms. The California mastitis test (CMT) and visual inspection were used to determine the prevalence of subclinical and clinical mastitis. Result: The total cow-level prevalence was 68.50% (89/130), with clinical cases accounting for 3.10% (4/130) and subclinical cases accounting for 65.40% (85/130). The quarter-level prevalence of mastitis was 35.19% (183/520), with 3.08% (16/520) clinical cases and 32.11% (167/520) subclinical cases. The study found that parity, lactation stage and age were all significant intrinsic risk factors for mastitis (P less than 0.05). Additionally, cow cleanliness level, cleaning solution, wiping udder after washing and dry cow treatment were significant extrinsic risk factors linked with mastitis (P less than 0.05). Given the high prevalence of mastitis in this region, regular screening and treatment are crucial to minimize the risk of mastitis and enhance cow health.

Unravelling transmission of Mycobacterium avium subspecies paratuberculosis to dairy calves: results of a lifelong longitudinal study.

Johne's disease (JD) is a chronic disease of ruminants endemic in the UK and other countries and responsible for large economic losses for the dairy sector. JD is caused by Mycobacterium avium subspecies paratuberculosis (MAP), which typically infects calves that remain latently infected during a long period, making early detection of infection challenging. Cow to calf transmission can occur in-utero, via milk/colostrum or faecal-orally. Understanding of the different transmission routes to calves is important in informing control recommendations. Our aim in this longitudinal study was to measure the association between the transmission routes via the dam and the environment on a calf subsequently testing serologically positive for MAP. The study population comprised of 439 UK dairy calves from 6 herds enrolled between 2012 and 2013. These calves were followed up from birth until 2023. At birth individual calf data was captured. During follow-up, individuals entering the milking herd were quarterly tested for the presence of MAP antibodies using milk ELISA. Cox regression models were used to measure the association between exposure from the dam (in-utero and/or colostrum) or from the environment (long time in dirty yard) and time to first detection of MAP infection. An association between calves born to positive dams and probability of having a MAP positivetestresultremainedafter excluding potential MAP transmission via colostrum(Hazardratio:2.24;95% CI:1.14-4.41). Calves unlikely to be infected with MAP via the in-utero or colostrum route, had 3.68 (95% CI: 3.68 1.45-9.33) higher hazard of a positive test result when they stayed longer in a dirty calving area. The effect of the dam infection status on transmission to calves precedes the dam's seroconversion and persists after excluding the potential role of transmission via colostrum. The association betweentime spent in a dirty calving area and probability of a MAP positive test resulthighlights the role of environmental contamination as a source of infection in addition to the dam.

Sustainable Technological Advancements for Dairy Waste Water Treatment-A Review

The dairy industry has global importance due to its essential contribution towards human nutrition as well as high economic opportunities. There is a consistent and steep growth in India's dairy sector in the last few decades and now India is one of the major milk-producing countries in the world. Highstrength dairy wastewaters, if discharged without proper treatment, severely pollute the receiving water bodies. In this scenario 'Waste as a resource' concept has been gaining the interest of researchers. The present review delineates different technological advancements for treating dairy wastewater and compares which is the better option in terms of sustainability. In the aerobic process, advanced bioaugmented systems which use bacterial and fungal consortia were found to be effective and sustainable with biological oxygen demand removal of 78.7% and 74.7%. Physio-chemical methods using natural coagulants like Moringa oleifera (MO) and pomegranate peel powder were also found to be effective with 81.8 % nitrogen removal from dairy wastewater treatment. Phytoremediation using water fern (Azolla pinnata R. Br.) was found to be efficient with dissolved solids reduction efficiency of 71.56 %. The fluidized bed anaerobic reactor unit was found to generate a biohydrogen content of 35.72±9.43% when the OLR was 53.25± 7.81 kg. COD m–3d–1. Microbial fuel cells treatment of real dairy wastewater (RDW) using Shewanella algae (MTCC-10608) has generated maximum current density and power density of 141 mA/m2 and 50 mW/m2 respectively. These two technologies are better sustainable options for dairy wastewater treatment since waste to energy aspect is satisfied.

Economic study of the milk value chain through the Kiganda-Bujumbura Mairie circuit in Burundi

The value chain (VC) analysis of commodity chains is a strategic tool made for measuring, developing and maintaining sustainable a commodity. This paper analyzes the economic dimension of the milk VC through the Kiganda-Bujumbura Mairie circuit in Burundi. Using primary data, a distribution of profit margins between actors was assessed. This survey involved a sample of 67 actors. The sampling technique used was the "snowball" method due to the lack of a sampling frame of actors. Secondary data were also collected in order to complete the primary data. The analysis was carried out using general and quantitative tools compatible with the subject matter. The results show that retailers benefit an average monthly profit margin of 201 Burundian franc (BIF) [US$0.097] per liter with an average monthly turnover of 1,818,733BIF [US$879.48]. Dairy farmers have a monthly profit margin of 187BIF [US$0.090] per liter. Their average monthly turnover is 134,894BIF [US$65.23]. The collectors have a profit margin of 78BIF [US$0.037] per liter with an average monthly turnover of 1,815,000BIF [US$877.67] and the wholesalers have a profit margin of 47BIF [US$0.022] per liter with an average monthly turnover of 7,257,480BIF [US$3,509.52]. Low margins are mainly due to the small quantities of milk produced and high production costs. Dairy farmers breed local breeds, with the constraints of balanced feed. The problem of suitable transport and processing equipment also affects the quality of the milk. Small dairy farm would benefit from forming associations and/or cooperatives. Being in associations and/or in cooperatives, they should be able to breed the improved breed, benefit from other advantages such as training, easy access to veterinary services, agricultural financing credits, etc. Public authorities must at all times monitor compliance with standards governing the dairy sector, and introduce regulatory measures if necessary.

A Study on Gross Profit Ratios of Leading Dairies of Gujarat

In general economy of any of the nation is tends to the three sectors – agriculture, industries and territory services. Food industry is one of the leading part an agriculture sector. Moreover, Gujarat is the leading player in milk production worldwide. This research is targeted to examine profitability in terms of Gross profit of the dairy sector of the Gujarat state. In this research the secondary data of all leading dairies of Gujarat have been collected to evaluate the gross profit of the industry. In sense of accounting technique an application of gross profit is examined to know the financial growth of the sector. An application of descriptive statistics and inferential statistics are used to derive the results. The results are compared bases on mean, standard deviation and ANOVA. It is observed that Amul is the leading player amide all the dairy. Thus, Amul model is approached to all other dairy.

Challenges in Evaluating Quality of the Serbian Traditional Dairy Product Kajmak

Kajmak, a traditional Serbian dairy product, is a top, crust layer of milk containing a high amount of aggregated milk fat and proteins, formed during long cooling of boiled milk. Due to variations in production technologies, environmental conditions, and the composition of raw milk which varies from region to region, the quality of kajmak produced traditionally in households is very inconsistent. Attempts to produce kajmak in the industry environment (dairy plants) turned out to be highly challenging in terms of achieving a uniform quality and safety of the product. In order to have a deeper insight into the quality of kajmak produced by the traditional manufacture and the industry, the main objective of the study was to investigate sensorial, physicochemical, and textural properties of kajmak produced in households and dairy plants and distributed through open markets and retail stores, respectively. A total of 36 samples of kajmak were analyzed for thirteen quality characteristics. The results obtained showed a high variability in kajmak quality. The study applied a mathematical calculation of a single total quality index (TQI) that makes all quality attributes comparable. Based on the obtained results, it was determined that kajmak produced in households had a better TQI, which leads to the conclusion that in terms of kajmak quality, the traditional production method is still superior to the industrial one. The correlation between the examined quality characteristics was investigated using Pearson’s bivariate correlation analysis and principal component analysis (PCA). By applying PCA, four principal components highlighting the most important quality characteristics were extracted. The results of the study suggest that further research on the quality of this valuable dairy product, as well as government support and investment in the small scale dairy sector, could be beneficial.

Life cycle assessment of greenhouse gas emission from the dairy production system - review.

Climate change is altering ecological systems and poses a serious threat to human life. Climate change also seriously influences on livestock production by interfering with growth, reproduction, and production. Livestock, on the other hand, is blamed for being a significant contributor to climate change, emitting 8.1 gigatonnes of CO2-eq per year and accounting for two-thirds of global ammonia emissions. Methane (CH4), nitrous oxide (N2O), and carbon dioxide (CO2) are three major greenhouse gases (GHG) that are primarily produced by enteric fermentation, feed production, diet management, and total product output. Ruminants account for three-quarters of total CO2-equivalent (CO2-eq) emissions from the livestock sector. The global dairy sector alone emits 4.0% of global anthropogenic GHG emissions. Hence, dairy farming needs to engage in environmental impact assessment. Public concern for a sustainable and environmentally friendly farming system is growing, resulting in the significant importance of food-based life cycle assessment (LCA). Over the last decade, LCA has been used in agriculture to assess total GHG emissions associated with products such as milk and manure. It includes the production of farm inputs, farm emissions, milk processing, transportation, consumer use, and waste. LCA studies on milk production would assist us in identifying the specific production processes/areas that contribute to excessive greenhouse gas emissions when producing milk and recommending appropriate mitigation strategies to be implemented for a clean, green, and resilient environment.

Enhancing individual animal resilience to environmental disturbances to address low productivity in dairy cattle performing in sub-Saharan Africa

The current review examines potential solutions to enhance the sustainability and productivity of the dairy sector in sub-Saharan Africa (SSA) with an emphasis on breeding for resilience. Additionally, the paper explores various indicators for measuring resilience and provides insights into the data that can be utilized to quantify resilience in SSA’s dairy production systems. Dairy production contributes significantly to food and nutritional security and employment in SSA. However, besides the general lack of enabling policy and institutional environments, production is negatively affected by environmental challenges such as high temperatures and heat stress, diseases and parasites, unreliable rainfall patterns, shortages of feeds and forages and undue preference for taurine cattle breeds regardless of their poor adaptability to prevailing local conditions. Fostering the resilience capacity of dairy animals is imperative to combat climate-related adversities and maintain productivity. This can only be achieved if reliable and practical methods for quantifying and analyzing resilience in SSA are described and undertaken. This study has reviewed variance of deviations, root mean square of deviations, autocorrelation of deviations, skewness of deviations, slope of the reaction norm and its absolute value as possible indicators of resilience in SSA. While previous research has reported genetic variation and favorable correlations of these indicators with health, fitness, and fertility traits, their potential in SSA environments requires further investigation. Besides, labor- and cost-effective phenotypic data collection is essential for characterization of resilience using these indicators. Through this study, we propose frequently collected data on milk production traits, body fat-related traits, and activity patterns as suitable in the sub-Saharan Africa context. The African Asian Dairy Genetic Gains Project by the International Livestock Research Institute (ILRI) offers a valuable opportunity to collate data from diverse dairy systems in SSA for testing the potential of these indicators. Insights from this study are helpful in improving resilience of dairy animals in SSA, which would contribute to poverty alleviation, animal welfare improvement, and better preparedness in lieu of climate change in SSA.

Inulin Functionalized "Giuncata" Cheese as a Source of Prebiotic Fibers.

The development of functional foods in the dairy sector represents a flourishing field of technological research. In this study, an Italian fresh cheese as "giuncata" was enriched with inulin, a dietary fiber, with the aim of developing a product with improved nutritional properties in terms of prebiotic action on intestinal microbiota. An inulin concentration of ~4% w/w was determined in the fresh cheese after the fortification process, enabling the claim of being a "source of dietary fiber" (inulin > 3 g/100 g) according to the European regulation. The addition of inulin has no effect on the pH of cheese and does not relevantly influence its color as well as the total fat content (fat reduction ~0.61%) in comparison to the control. Mechanical properties of the cheese were also not markedly affected as evidenced from rheological and tensile testing analyses. Indeed, the incorporation of inulin in "giuncata" only exerts a slight "softening effect" resulting in a slightly lower consistency and mechanical resistance in comparison to the control. Overall, this study demonstrates the feasibility of producing a fiber-enriched dairy functional food from a large consumed fresh and soft cheese as "giuncata".

State support of the dairy industry and prospects for its development in the post-war period

Purpose. The article aims – to identify current trends, determine the level of state support and prospects for the development of the dairy industry in Ukraine in the post-war period, taking into account cooperation with the EU.&#x0D; Methodology / approach. The research was conducted using the comparison method with the nearest EU country – Poland. The analysis of indicators of the development of the dairy industry was carried out on the basis of the data of the State Statistics Service of Ukraine, Poland, Eurostat, and Statista company using methods of relative values, a series of dynamics.&#x0D; Results. Analysis of the dynamics of the main dairy industry indicators in Ukraine revealed a long-term trend towards a decrease in milk production volumes. During 1990–2021, the volume of milk production decreased by 3 times, and the number of cows decreased by 5 times. Since 2020, the dairy industry has shifted from being export-oriented to becoming dependent on imports. On the other hand, Poland, which is geographically and climatically close to Ukraine, shows the opposite dynamics of the dairy industry development. Studying the functioning of the dairy sector in Poland revealed a high level of state financial support and fiscal stimulation. The study shows that the financial support of the dairy industry of Ukraine is noticeably insufficient, and the fiscal stimulation provided is inadequate. Moreover, the efficiency of managing budget funds is low. The situation is further complicated by the ongoing military aggression from russia. Considering the constraints of state funding, it is suggested that the array of instruments for supporting animal husbandry should be tailored based on the scale of entrepreneurial entities involved; differentiation in the provision of state support should also apply to agricultural producers in the de-occupied territories. In order to fully and promptly manage the amount of financing, it is necessary to speed up the procedure for distributing funds between areas and approving financial documentation; it is expedient to consider the possibility of payments for the keeping dairy cattle for small business entities without the need for coordination with the main manager of funds.&#x0D; Originality / scientific novelty. The obtained results allow a deeper investigation of the main development issues of the Ukrainian dairy industry in the pre-war period and under martial law in the context of further European integration.&#x0D; Practical value / implications. The study’s findings can be the basis for further scientific research in the direction of developing a comprehensive set of measures to stimulate Ukraine’s dairy industry. This is of particular importance in the post-war period promoting the restoration of food security.

Comparative study of depotash vinasse and molasses as binder and production performance in lactating Murrah buffaloes.

Dairy sector has recently focused a lot of attention on the addition of agricultural by-products as functional feed additives as an environmentally friendly and sustainable technology. Depotash vinasse (DPV) serves as a cheap source of nutrients and a binder for animal feed in dairy sector. However, there is little information available on the usage of depotash vinasse on animals. Therefore, the aim of the present study was to assess the role of depotash vinasse as pellet binder on nutrient digestibility, blood parameters and milk production in early lactating Murrah buffaloes. Fifteen Murrah buffaloes (daily milk yield 8.5 to 9.0kg/day) were randomly assigned to three groups, viz., control, group 1 (G1) and group 2 (G2) on the basis of milk yield and days in milk. The control group animals received a basal diet of concentrate mix, oat greens and wheat straw, G1 animals received molasses as a binder (8%), while G2 received DPV as binder (8%). Results revealed that there was no significant effect on nutrient digestibility. Blood parameters and hepatic enzymes were statistically similar (P > 0.05). Supplementation of depotash vinasse as binder had no effect on plasma minerals and was comparable to control group. There were no changes in milk production and 6% fat-corrected milk yield in treated groups as compared to control. It was concluded that depotash vinasse (8%) may be used for pellet production with no negative impact on milk yield and composition, nutrient digestibility and blood biochemical parameters in early lactating buffaloes.

Comparison and interobserver reliability between a visual analog scale and the Wisconsin Calf Health Scoring Chart for detection of respiratory disease in dairy calves

Respiratory disease is an ongoing challenge for calves in the dairy sector with a relatively high prevalence and impact on welfare and economics. Applying scoring protocols for detecting respiratory disease requires that they are easily implemented, consistent between observers and fast to use in daily management. This study was conducted in one Danish dairy farm from September 2020 through January 2021. The study included 126 heifer calves enrolled in the age of 17 to 24 d. All calves were observed every second day for a period of 46 d. At each visit all calves were scored with a new Visual Analog Scale (VAS) and the Wisconsin Calf Health Scoring Chart (WCHSC). We calculated agreement between the 2 scoring systems based on conditional probability to score higher or lower than a cut-off in the VAS compared with a specified cut-off in WCHSC used as reference test. A generalized mixed effects regression model was performed to develop a model that could estimate the prevalence of respiratory disease and the overall agreement between the 2 scoring systems. The overall agreement between the VAS and WCHSC was 89.6%. The second part of the study assessed inter-observer reliability between 2 experienced observers and between an experienced observer and veterinary students. The inter-observer reliability was calculated by intra-class correlation coefficient and was 0.58 between experienced observers and was 0.34 between an experienced observer and veterinary students indicating a moderate to poor reliability between the observers. It was possible to use VAS as an alternative clinical scoring method, which primarily focuses on the general condition of the individual calf rather than specific categories of clinical signs. Our study set up lacked a comparison to other diagnostic tools i.e., thoracic ultrasound to confirm the findings which should be considered in future studies when exploring VAS as a screening tool for detection of respiratory disease in dairy calves.

Exploring the sources of variation of electrical conductivity and total and differential somatic cell count in Italian Mediterranean buffaloes

In the buffalo dairy sector, a huge effort is still needed to improve mastitis prevention, detection, and management. Electrical conductivity (EC) and total somatic cell count (SCC) are well-known indirect indicators of mastitis. Differential somatic cell count (DSCC), which represents the proportion of neutrophils and lymphocytes on the total SCC, is instead a novel phenotype collected in the dairy cattle sector in the last lustrum. As little is known about this novel trait in dairy buffalo, in the present study we explored the nongenetic factors affecting DSCC, as well as EC and total somatic cell score (SCS), in the Italian Mediterranean buffalo. The data set used for the analysis included 14,571 test-day (TD) records of 1,501 animals from 6 herds, and climatic information of the sampling locations. The original data were filtered to exclude animals with less than 3 TD per lactation and, for the investigated traits, outliers beyond 4 standard deviations. In the statistical model we included the fixed effects of herd (6 classes), days in milk (DIM; 10 classes of 30 d, with the last being an open class until 360 d), parity (6 classes, from 1 to 6+), year-season of calving (11 classes, from summer 2019 to winter 2021/2022), year-season of sampling (9 classes, from spring 2020 to spring 2022), production level (4 classes based on quartiles of average milk yield by herd), and temperature-humidity index (THI; 4 classes based on quartiles, calculated using the average temperature and relative humidity of the 5 d before sampling). Average EC, SCS, and DSCC vary across herds. Considering DIM, greater EC values were observed at the beginning and the end of lactation; SCS was slightly lower, but DSCC was greater around the lactation peak. Increased EC, SCS, and DSCC levels with increasing parity were reported. Year-season calving and year-season sampling only slightly affected the variation of the investigated traits. Milk of high-producing buffaloes was characterized by lower EC and SCS mean values, nevertheless it had slightly greater DSCC percentages. Buffaloes grouped in the highest THI classes (classes 3 and 4) showed, on average, greater EC, SCS, and DSCC in comparison to the lower classes, especially to class 2. Results of the present study represent a preliminary as well as necessary step for the possible future inclusion of EC, SCS, or DSCC in breeding programs aimed to improve mastitis resistance in dairy buffaloes.