Daily Water Research Articles

418 Evaluation of an automated system for measuring individual animal water consumption

Abstract SmartWater is an automatic watering station that measures the volume of water consumed by individual animals when visiting the system. The self-contained, portable system is constructed of a steel frame, two load cells, and a stainless-steel water trough that measures 0.1 m3 with a water capacity of 113 L. SmartWater refills automatically when the water mass is below 55 L, refilling for 120 s, or until the water mass reaches 70 L. The flow rate of water entering the system and the weight of the bin are measured every second in L/s and L, respectively. When the bin is being refilled, animals are still able to drink from the system. Animal water intake during a visit is measured by summing the amount of water dispensed into the bin and the difference in bin weight at the start and end of the visit. Water intake days are considered valid if the unknown percentage is less than 5% of the total daily water intake, where unknown intake is defined as feed mass disappearance that cannot be assigned to an EID. Invalid days are excluded from final datasets. System performance was evaluated using 17 Angus heifers (body weight = 248 ± 37 kg) in a 55-d trial period. Animals were given ad libitum access to two SmartWater systems, and all animals successfully adapted to the system. Mean daily visitation was 2.7 ± 0.4 and average visit length was 87 ± 175 s. Mean water consumption over the trial period was 13.7 ± 1.8 L. The coefficient of variance of water consumption between animals was 12.8%. The average standard deviation water consumption within animals between days was 5.9%. SmartWater provides an accurate and efficient way to measure water intake of animals in extensive and intensive systems, eliminating the need to manually monitor water levels and refill watering stations.

Combining price and non-price interventions for water conservation

Abstract Marginal pricing has long been the instrument of choice to address water conservation challenges. More recently, non-price behavioral interventions have emerged as an alternative. However, there is limited data on the relative efficacies of price and non-price interventions. We report results from long-term field experiments studying unit-level water conservation responses to both price and non-price interventions in the same group of households (n = 64 186 household-days). Conservation habits, attitude-action gaps, principal-agent incongruities, and billing cycles help account for the heterogeneity in response between households, and across time. A non-price behavior modification intervention before the introduction of marginal pricing resulted in a large and significant effect on treated households (33%). The subsequent introduction of marginal volumetric pricing also reduced water use (8%, for previously untreated households). However, this average price effect masks how a large share (21%) of households increased water use, or how a mere 12% of the households accounted for all the aggregate reduction in water use. We investigated such heterogeneous responses as a systematic conservation maximization design question beyond statistical variance in individual responses. We used daily water consumption measurements across three years alongside a household survey to delineate structural and agentic barriers to conservation behavior. Our analysis reveals how combining price and non-price behavioral interventions could hold the key to achieving conservation effects that are both large and persistent.

Local neighborhood affects stem rehydration under drought: evidence from mixtures of European beech with two different conifers.

Mixed-species forests are, for multiple reasons, promising options for forest management in Central Europe. However, the extent to which interspecific competition affects tree hydrological processes is not clear. High-resolution dendrometers capture sub-daily variations in stem diameter; they can simultaneously monitor stem growth (irreversible changes in diameter) and water status (reversible changes) of individual trees. Using the information on water status, we aimed to assess potential effects of tree species mixture, expressed as local neighborhood identity, on night-time rehydration and water stress. We deployed 112 sensors in pure and mixed forest stands of European beech, Norway spruce, and Douglas fir on four sites in north-western Germany, measuring stem diameter in 10-minute intervals for a period of four years (2019-2022). In a mixture distribution model, we used environmental variables, namely soil matric potential, atmospheric vapor pressure deficit, temperature, precipitation, and neighborhood identity to explain night-time rehydration, measured as the daily minimum tree water deficit (TWDmin). TWDmin was used as a daily indicator of water stress and the daily occurrence of sufficient water supply, allowing for stem growth (potential growth). We found that species and neighborhood identity affected night-time rehydration, but the impacts varied depending on soil water availability. While there was no effect at high water availability, increasing drought revealed species-specific patterns. Beech improved night-time rehydration in mixture with Douglas fir, but not in mixture with spruce. Douglas fir however, only improved rehydration at a smaller share of beech in the neighborhood, while beech dominance tended to reverse this effect. Spruce was adversely affected when mixed with beech. At species level and under dry conditions, we found that night-time rehydration was reduced in all species, but beech had a greater capacity to rehydrate under high to moderate soil water availability than the conifers, even under high atmospheric water demand. Our study gives new insights into neighborhood effects on tree water status and highlights the importance of species-specific characteristics for tree-water relations in mixed-species forests. It shows that drought stress of European beech can be reduced by admixing Douglas fir, which may point towards a strategy to adapt beech stands to climate change.

Techno-economic assessment of vertical axis wind turbine driven RO desalination with compressed air energy storage for remote communities

Renewable energy desalination is gaining much attention in remote off-grid communities facing challenges in accessing clean water. Typically, batteries ensure the continuous operation of small-scale renewable reverse osmosis (RO) desalination systems; however, they are expensive and have relatively shorter lifespans. This study investigates the implementation of a compressed air energy storage (CAES) system coupled with a vertical axis wind turbine (VAWT) to directly drive small-scale RO desalination, potentially replacing batteries and reducing energy conversions. A Simulink model was developed to simulate the performance of a VAWT-driven CAES operating RO units, adaptable for both technical and economic assessments. Parametric studies have identified the optimal configuration. The most cost-effective configuration, utilising eleven VAWTs and a pressure exchanger (PX), achieves a levelised cost of water (LCOW) of 1.63 US$/m3 and an annual water production of 9400 m3. The normalised daily water production per square metre of turbine swept area at the study site is 0.19 m3/m2/day at an average wind speed of 5 m/s. While this configuration has a higher initial capital cost, it yields the lowest LCOW. The CAES system effectively addresses the intermittency challenges of wind energy. This study presents a novel, battery-free VAWT-CAES-RO system as a sustainable desalination solution for remote communities, offering a promising approach to address water scarcity in an environmentally friendly manner.

Synergistic ionic liquid encapsulated MIL-101 (Cr) metal-organic frameworks for an innovative adsorption desalination system

The transformations of brackish water into freshwater employing porous materials such as metal-organic frameworks (MOFs) show a great potential for a green environment. But the adsorption-based desalination slows down due to slow water adsorption/desorption rates and inadequate water storage capacity in conventional adsorbents. Therefore, the restructuring of porous MOFs increases water storage and transfer rates. This paper presents a comprehensive investigation on the fabrication of ionic liquid encapsulated MIL-101 (Cr) MOFs (metal-organic frameworks), and its interactions with water for desalination applications. Firstly, the pristine MIL-101 (Cr) is encapsulated with various types/amounts of ionic liquids. Next, these MOFs are characterized via SEM (Scanning electron microscope), FTIR (Fourier transform infrared), XRD (X-ray diffraction), TGA (Thermal gravimetric analysis), N2 adsorption techniques. The water adsorption on these MOFs is performed experimentally for a wide temperature (30–80 °C) and pressure (0 < P/Ps < 0.95) ranges. The effects of the size (or type) as well as the encapsulation ratio of ionic liquids on water adsorption behaviors are conducted. Based on the experimentally proven water adsorption (isotherms and kinetics) data, an AD (adsorption desalination) system is modelled and simulated. Finally, the AD performances in terms of the specific daily water production (SDWP) and performance ratio (PR) are parametrically studied with respect to various regeneration temperature (50–80 °C) and cycle times (100 s–1000 s). It is found that by ionic liquid encapsulation, the water uptakes/offtake rates are expedited up to 48% (for adsorption)/55% (for desorption), respectively. In addition, the water transfer (Δq) improves up to 45% more than the parent MIL-101(Cr) MOFs. Furthermore, the SDWP increases from 38 to 56 m3 of water per tonne of MOFs per day at the regeneration temperature of 70 °C. The simulation results also show that the ionic-liquid-encapsulated MIL-101 (Cr) MOFs generates water (>25 m3 water per tonne of IL-MOFs) at the regeneration temperature of 50 °C and is potential for the next generation desalination applications.

Behavior of LSTM and Transformer deep learning models in flood simulation considering South Asian tropical climate

ABSTRACT The imperative for a reliable and accurate flood forecasting procedure stems from the hazardous nature of the disaster. In response, researchers are increasingly turning to innovative approaches, particularly machine learning models, which offer enhanced accuracy compared to traditional methods. However, a notable gap exists in the literature concerning studies focused on the South Asian tropical region, which possesses distinct climate characteristics. This study investigates the applicability and behavior of long short-term memory (LSTM) and transformer models in flood simulation considering the Mahaweli catchment in Sri Lanka, which is mostly affected by the Northeast Monsoon. The importance of different input variables in the prediction was also a key focus of this study. Input features for the models included observed rainfall data collected from three nearby rain gauges, as well as historical discharge data from the target river gauge. Results showed that the use of past water level data denotes a higher impact on the output compared to the other input features such as rainfall, for both architectures. All models denoted satisfactory performances in simulating daily water levels, with Nash–Sutcliffe Efficiency (NSE) values greater than 0.77 while the transformer encoder model showed a superior performance compared to encoder–decoder models.

Evaluation of a solar-driven adsorption desalination system for Brazilian semiarid region

In this study, the effect of using a hybrid solar thermal-activated adsorption desalination system for brackish water is evaluated under the climatic conditions of the Brazilian semiarid region. The proposed theoretical model utilizes climatic data from the meteorological station in Campina Grande, PB, and adsorptive kinetics data of Fuji Davison RD 260 silica gel to predict the performance indices of the specific daily water production (SDWP), specific cooling power (SCP), and coefficient of performance (COP) performance coefficients over a characteristic day. The SDWP value of 6.26 m3/ton, SCP ranging from 50 to 300 W/kg, and an average COP of 0.5 were obtained, considering variations in global horizontal irradiance in the ACDS system and transient ambient temperature. It was observed that both the production of desalinated water and the refrigeration effect increase with the rise in daily solar irradiance. The variation in the number of solar collectors used in the system and their optimality, as well as the variation in the salinity index of the feed source, impacted the evaluated performance coefficients.

Association between predicted level of water turnover deficit and all-cause and cause-specific mortalities among Japanese adults: The Japan Collaborative Cohort Study

BackgroundAlthough water is essential for maintaining health and life, the association between water turnover as an indicator of daily water requirement and water deficit and mortality is unclear. ObjectivesWe aimed to investigate this association in Japanese adults. MethodsA total of 63,488 (36,739 females and 26,749 males) Japanese aged 40–79 y in the Japan Collaborative Cohort Study in 1988–1990 were followed up their mortality through 2009. Water turnover was calculated considering lifestyle and environmental factors using an equation previously developed by the International Doubly Labeled Water Database Group. Participants were classified into sex-specific quintiles based on their water turnover. Water deficit levels were calculated from water turnover and dietary water consumption, which were estimated using a validated questionnaire. ResultsThe average daily water turnovers were 2950 and 3466 mL in females and males, respectively. During 19.4 years of median follow-up (1,039,914 person-years), 12,551 deaths were recorded. After adjusting for lifestyle and medical history, the bottom quintile was associated with higher hazard ratios (HRs) of mortality from all causes (females—HR: 1.26; 95% CI: 1.12, 1.41; males—HR: 1.18; 95% CI: 1.07, 1.29) and cardiovascular disease (CVD). In spline analyses, the water turnover range with the lowest HR for all-cause mortality was 3000–3300 mL/d in females and the water turnover at which the HR for all-cause mortality plateaued was ∼3500–3700 mL/d in males. Water deficiency levels were associated with all-cause and CVD-related mortality in both sexes. ConclusionsThe association between water turnover and deficit and all-cause and CVD-related mortalities (especially coronary artery disease and ischemic stroke) in adults is L-shaped. These findings may be useful for setting the target values of water requirement, and differences between dietary requirements and actual intake may bridge the knowledge gap in water–mortality associations.

Variable frequency constant pressure water supply system based on Siemens S-1200

Abstract This paper presents the design of an efficient, cost-effective, and intelligent variable frequency water supply control system. The system consists of a PLC, frequency converters, water pumps, pressure sensors, and other components to form a closed-loop control system. It enables closed-loop control of water pressure, ensuring a dynamic balance between water consumption and water supply. A user-friendly HMI is designed based on a software platform for easy operation by management personnel. It allows for seamless switching of the system’s operating modes and the running status of water pumps. Real-time display of pump operation and monitoring of system performance are also provided. Compared to traditional water supply methods, this system exhibits superior reliability, speed, and performance. It is capable of meeting the daily water consumption needs of residents in high-rise buildings.

Evaluation of the Effect of Soil Water Conditions on the Development and Water Requirements of Adult Oil Palm (Elaeis guineensis Jacq.) in the Northern Region of Colombia

Sustainable water management is a key approach for enhancing the productivity of oil palm trees while addressing the impacts of climate change and variability. Determining the water needs of a crop is crucial for the appropriate application of water. This research was carried out in two plantations in Agustín Codazzi, Colombia, using a completely randomized design. This study involved examining the impacts of five different water conditions (50, 150, 300, 450, and 600 L per day) on the growth and yields of mature oil palm trees (aged 10–17 years), and their water consumption was calculated using the water balance method. The results indicated that the crop was negatively affected by daily water applications of 300, 150, and 50 L per day, showing statistically significant differences (p &lt; 0.05) when compared to the 450 and 600 L per day treatments, particularly in terms of leaf emergence, leaf area index, and yield (tons per hectare). The 50 L per day treatment resulted in the most substantial decrease in yield (around 26%), primarily attributed to a reduction in the number of bunches. The most favorable crop responses were observed with water applications of 450 and 600 L per day, aligning with the crop’s potential evapotranspiration values (ranging between 5.4 and 5.7 mm per day) and yielding crop coefficients of 0.88 and 0.9, respectively. Notably, these values varied between dry and rainy seasons, peaking between December and March.

Spatial and temporal analysis of daily terrestrial water storage anomalies in China

Spatial and temporal analysis of daily terrestrial water storage anomalies in China

Factors affecting the oral health of patients on maintenance hemodialysis and recommendations for standardized nursing care: a multicenter study.

To determine the oral health status of patients on maintenance hemodialysis and to identify the factors influencing their oral health. This observational study included 1,186 patients with chronic kidney disease who received maintenance hemodialysis across 33 hospitals in China. The patients were recruited for a questionnaire survey between April and August 2023 at Beijing Chaoyang Hospital using stratified sampling. Data collection tools included the General Information Questionnaire for Maintenance Hemodialysis Patients, the Oral Health Assessment Tool, the Pittsburgh Sleep Quality Index, and the Hospital Anxiety and Depression Scale. Spearman rank correlation coefficients were used to assess the relationships between the oral health of patients on maintenance hemodialysis and continuous variables such as sleep quality and emotional status. Multiple linear regression analysis was employed to explore the relationship between oral health and various variables. The oral health scores of the patients ranged from 8 to 22, with a mean score of 12.54 ± 2.63. The final model of the multiple linear regression analysis indicated a goodness of fit of 22.19%. Independent factors affecting the oral health of patients included smoking, the proportion of medical expenses, water consumption, sleep quality, and anxiety scores (all P .05). High levels of smoking, substantial medical expenses, poor sleep quality, and elevated anxiety scores were risk factors for poor oral health (all P .05). Adequate daily water intake served as a protective factor for oral health (P .05). This study proposes targeted interventions to enhance the management and improvement of oral health in patients on hemodialysis, aiming to provide highly personalized and effective oral health care. These interventions are expected to improve oral health outcomes in future clinical practice.

Growth of Tilapia (Oreochromis niloticus) Juvenile strain Nirwana with Different Natural Feeding

The high cost of commercial feed in Nirwana tilapia aquaculture leads to elevated production costs and reduced profits. As a solution, the provision of natural feed is proposed as an alternative to reduce production expenses. This study aims to compare the growth of Nirwana tilapia fed with three different natural feeds: Tubifex sp., Chironomus sp. larvae, and maggots. The experiment was conducted using a completely randomized design with three replicates for each treatment. The feeding rate was 3% of the fish’s body weight, administered three times a day. The parameters measured included absolute biomass, absolute length gain, daily growth rate (Specific Growth Rate, SGR), survival rate (SR), and water quality during the 30-day rearing period. The results showed that feeding Nirwana tilapia with Tubifex significantly increased growth compared to the control group (commercial pellets), Chironomus larvae, and maggots. The absolute biomass from Tubifex feeding was 5.23 ± 0.89 g, absolute length was 4.2 ± 7.8 cm, SGR was 1.52 ± 0.33%, and SR was 90%. The next highest growth was seen in Chironomus larvae, followed by maggots, with the control yielding the lowest results.

Potential Amplifiers of Hydrological Drought: A Deep Dive into the Seasonal Variations of New, Young, and Old Water Fractions in the Stream Discharge of a Pre-Alpine Catchment

This study examines runoff generation processes by focusing on new, young, and old water fractions in the stream discharge within the Swiss pre-Alpine Alp catchment and its smaller tributaries, Erlenbach and Vogelbach. Young water represents a contribution from the three most recent rainfall-runoff events. This research utilises a four-year time series of daily stable water isotope data from stream water and precipitation to investigate seasonal variations. An extended two-component hydrograph separation method is applied to analyse the catchment water and tracer mass balance across both the event and pre-event time axes. The calculated new, young and old water components in stream discharge help estimate time-varying backward travel time distributions. Factors such as air temperature, snow depth, and evapotranspiration data are considered to understand isotope fractionation because of phase changes. The results suggest that discharge generated in autumn and winter may have longer backward travel times compared with spring or summer discharge. Additionally, forest cover influences water fractions older than 40 days. The study identifies a nivo-pluvial runoff regime, with new water fractions peaking in August (Erlenbach, Vogelbach) and January (Alp). Young water fractions reach their highest levels at the start of winter and during snowmelt, while old water fractions peak towards the end of snowmelt and in mid-autumn. The Alp catchment and its tributaries exhibit higher new and young water components than old water following a month of low precipitation and high evapotranspiration in July. These findings are significant in light of the projected drier summers in Central Europe.

Longitudinal propagation of aquatic disturbances following the largest wildfire recorded in New Mexico, USA

Wildfire disturbance propagation along fluvial networks remains poorly understood. We use incident, atmospheric, and water-quality data from the largest wildfire in New Mexico’s history to quantify how this gigafire affected surface runoff processes and mobilized wildfire disturbances into fluvial networks after burning 1382 km2. Surface runoff post-fire increased compared to pre-fire conditions, and precipitation events that are frequently observed in the affected watershed (<2-year recurrence) and fell during the post-fire first rainy season resulted in uncorrelated, less frequently observed runoff events (10-year recurrence). Besides these shifts in runoff generation, the magnitude and fluctuation of daily water quality parameters and relevant ecosystem processes also shifted over multiple months, even at sites located >160 km downstream of the burn perimeter. Our findings emphasize the need to incorporate spatially resolved longitudinal sampling designs into wildfire water quality research and highlight the spatiotemporal co-dependency among atmospheric, terrestrial, and aquatic processes in defining the net outcome of wildfire disturbance propagation along impacted fluvial networks.

Development of a new test design to investigate the degradation of pesticides in soil under sunlight conditions

Pesticides applied to soil surface are subject to photodegradation if the parent molecule is sensitive to UV-light absorption. Photodegradation studies are therefore mandatory for the registration of plant protection products to provide data on the degradation rate and on the nature of photoproducts formed. In general, sunlight is simulated in these studies with xenon lamps, e.g., a Suntest® device. Surface application on very thin soil layers followed by direct irradiation is common practice, but the control of the boundary conditions, i.e. soil temperature and moisture, to maintain the structure and viability of the soil is challenging. A homogeneous and stable soil microclimate is crucial to compare the degradation data of the test item from the irradiated soil samples to the dark controls as well as to the results from the aerobic soil metabolism study. After trying different scale-up test systems with the UV-sensitive herbicide imazamox as comparative test item, a new soil photolysis test system was developed which is manageable in the laboratory and enables a more favorable management of the boundary conditions, especially with regard to the soil moisture and temperature. For this, the solar simulator SolarConstant® 1200, equipped with metal halide lamps Radium HRI-TS 1000W/D/S/PRO, was installed by Atlas Ametek (Germany) in a temperature controllable walk-in incubation chamber with aluminum racks and reflectors to minimize diffuse light and to maintain a homogenous temperature of 22(± 1)°C within the irradiated soil. Borosilicate glass vessels with an inner diameter of 10 cm and a maximum height of 9 cm, covered by quartz glass, were used for the incubation of the applied soil under light. Contrary to the imazamox degradation half-lives obtained with the Suntest® test system, where an unusual slower degradation was observed under light compared to the dark controls, the results from the new SolarConstant® study design showed the expected faster degradation under light. Hence, it can be concluded that the experimental boundary conditions of the new test system are more suitable to maintain the viablity of the irradiated soil. Since no adjustments of the soil water content were needed, compared to daily water adjustments for thin soil layers incubated under a Suntest®, drying–wetting cycles are eliminated and microbial-induced soil processes are maintained.

Perancangan Rangka Tandon Air Berkapasitas 1200 Liter Untuk Kebutuhan Pelaku Usaha Rumah Kost

The increasing public interest in the need for adequate housing, especially the boarding house business, is one of the facilities that has great opportunities every year. The need for clean water in a boarding house environment is a factor that needs to be considered because clean water is an important need in human life. The water reservoir frame design with a capacity of 1200 liters is an optimized solution to meet the daily water needs of boarding house residents. The aim of this research is to design a water reservoir frame for water loads and carry out structural calculations of the water reservoir frame in accordance with applicable safety and strength standards. The research methodology explains in detail the research method which includes time and place of research, tools and materials, research procedures and research flow diagrams. Weight of load to be supported by the structure = weight of tank + weight of water Specify tank Type = Penguin TB, 120 uk Capacity = 1200 Liters = 1.2 m3 Tank mass (mT) = 29 kg Total mass of external load (mtot) = tank mass + water mass Mtot= 29 kg + 1200 kg = 1229 kg Total weight (Wtot) Wtot = (mtot) × g, g= acceleration due to gravity (9.8 m/s2) Kata kunci: Water discharge, Frame Strength, Structural Strength, Steel Selection, Water Reservoir

Growth performance, biochemical outcomes, and testicular histological features in male Japanese quails supplemented with milk thistle seeds

BackgroundDue to its rich content of active phytochemicals, milk thistle is regarded as a promising nutritional supplement for quails, particularly in regions with limited financial resources. Thus, our study aimed to evaluate the possible beneficial impact of aqueous extract of milk thistle seeds (MTS) at two graded concentrations (10 and 20 ml/L of drinking water) on male Japanese quails during their reproductive period.ResultsThe phytochemical analysis revealed the presence of 29 active compounds, including nine flavonoids and ten phenolic compounds. The supplemented groups showed significant improvements in body weight gain. MTS1 group exhibited a notable decrease in daily feed consumption, while MTS2 group showed a significant increase in daily water consumption. There was a dose-dependent increase in cecum length. The total count of intestinal bacteria decreased in a dose-dependent manner. Incorporating aqueous extract of MTS at concentration of 10 ml /L resulted in a significant increase in total protein and packed cell volume. Similar increases in globulin and decreases in the albumin/globulin ratio and aspartate aminotransferase (AST) were observed with both doses of supplementation. A significant decrease in total cholesterol and AST was observed in the high-dose group. Significantly higher plasma testosterone and triiodothyronine levels were observed only in the high-dose group, while plasma thyroxine levels were similarly increased in both supplemented groups. Intervention with MTS resulted in dose-dependent increases in cloacal gland index and cloacal foam production. Both supplemented groups showed significant increases in the diameter of seminiferous tubules and the number of Sertoli cells.ConclusionMarked growth-promoting, antibacterial, and reproductive-enhancing effects were observed when incorporating aqueous extract of MTS into the quails’ drinking water, particularly at a dosage of 20 ml/L.

MOF-801 enabled rapid-cycling atmospheric water harvester with vertically aligned photothermal channel

Sorption-based atmospheric water harvesting (SAWH) with metal–organic frameworks (MOFs) offer a promising solution to freshwater scarcity in arid regions. However, given the powder nature of MOFs, scaling-up such a concept with industrially favorable monolithic MOFs in bulky sizes remains challenging. In this study, a pore vertically aligned nanocomposite sorbent where MOF-801 is anchored at the polymeric structure surface was prepared via Fe3+-induced crosslinking and directional freezing methods. The continuous vertically aligned hierarchical pores not only offer sufficient sites for MOF-801 loading but also provide rapid moisture transport channels, as well as high solar-thermal conversion efficiency, resulting in a rapid ab/desorption duration of 2 h (including an absorption process for 1 h and a desorption process for 1 h under 1 sun irradiation) and multicycle daily water production of 1.40 L kg−1 at low RH of 20 % and 5.57 L kg−1 at higher RH of 80 %, respectively, which is outperformed most of the state-of-the-art MOFs based SAWH materials. This work provides a simple and effective way to shorten MOF-801 ab/desorption duration, which has great means for designing of high-performance solar-driven water harvesting materials.

An integrative pharmacology-based study on the efficacy and mechanism of essential oil of Chaihu Guizhi Decoction on influenza A virus induced pneumonia in mice

Ethnopharmacological relevanceChaihu Guizhi Decoction (CGD) has a long history of use in China for the treatment of influenza, which involves the use of a variety of aromatic herbs. Our previous studies have found that the contents of aromatic constituents in CGD affected the efficacy of treatment of influenza-infected mice, suggesting a clue that essential oil from CGD may play a relatively important role in ameliorating influenza induced pneumonia. Aim of the studyTo evaluate the anti-influenza potential of essential oil derived from Chaihu Guizhi Decoction (CGD-EO), to characterize and predict the key active components in CGD-EO, and to explore the mechanism of action of CGD-EO. Materials and methodsCGD-EO was obtained by steam distillation, and the components of the essential oil were characterized by gas chromatography-mass spectrometry (GC-MS) in conjunction with the retention index. The constituents absorbed into the blood of mice treated with CGD-EO were analyzed by headspace solid phase microextraction gas chromatography/mass spectrometry (HS-SPME-GC/MS). The potential anti-influenza active constituents and their possible action pathway were predicted by simulation using a network pharmacology approach. The protective effect of CGD-EO and its major components on H1N1/PR8-infected cells was determined using the CCK8 assay kit. Mice infected with influenza A virus H1N1/PR8 were administered different doses of CGD-EO orally and the body weights and lung weights were recorded. Mice with varying degrees of H1N1/PR8 infection were administered CGD-EO orally, and their daily weight, water consumption, and clinical indicators were recorded. Necropsies were conducted on days 3 and 5, during which lung weights were measured and lung tissues were preserved. Furthermore, the mRNA expression of the H1N1/PR8 virus and inflammatory factors in lung tissue was analyzed using RT-qPCR. Results(E)-cinnamaldehyde was the most abundant compound in the CGD-EO. The results of serum medicinal chemistry combined with network pharmacological analysis indicated that (E)-cinnamaldehyde and 3-phenyl-2-propenal may be potential active components of the CGD-EO anti-influenza, and may be involved in the NF-κB signalling pathway. In vitro studies have demonstrated that both CGD-EO and cinnamaldehyde exert a protective effect on MDCK cells infected with H1N1/PR8. In a 0.5 TCID50 H1N1/PR8-induced influenza model, mice treated with CGD-EO at a dose of 63.50 μg/kg exhibited a reduction in lung index, pathological lung lesions, and H1N1/PR8 viral gene levels. In addition, CGD-EO treatment was found to regulate the levels of inflammatory cytokines, including IL-6, TNF-α, and IFN-γ. Moreover, following three days of administration, an upregulation of NF-κB mRNA levels in mouse lung tissue was observed in response to CGD-EO treatment. ConclusionsThe findings of our study indicate CGD-EO exerts a protective effect against H1N1-induced cytopathic lesions in vitro and is capable of alleviating H1N1-induced pneumonitis in mice. Moreover, it appears to be more efficacious in the treatment of mild symptoms of H1N1 infection. Studies have demonstrated that CGD-EO has antiviral potential to attenuate influenza-induced lung injury by modulating inflammatory cytokines and NF-κB signalling pathways during the early stages of influenza infection. It is possible that (E)-cinnamaldehyde is a potential active ingredient in the anti-influenza efficacy of CGD-EO.