Daily Variation Research Articles

Investigating home-based opioid use among youth with sickle cell disease using ecological momentary assessment.

Opioids are a common and essential treatment for acute sickle cell disease (SCD) pain. However, opioids carry well-known adverse side effects, including potential development of hyperalgesia and nociplastic pain. We characterized opioid use in youth with SCD using ecological momentary assessment (EMA) data, and investigated the relationships between home-based opioid use, pain, and a range of biopsychosocial factors. Eighty-eight youth with SCD (aged 8-17years) completed EMAs assessing home-based opioid use, pain, and related factors. Analyses consisted of descriptive and multilevel logistic regression to predict daily home opioid use. Youth averaged 3.64weeks of EMAs. Approximately 35% of the sample (n=31) took an opioid during the EMA period, and used them on only 24% of reported pain days. Youth who took opioids reported a higher percentage of pain days (t=-2.67, p<.05) and mean pain severity scores (t=-2.30, p<.05) than youth who did not take opioids. Multilevel logistic regression analyses indicated that high daily pain severity (odds ratio [OR]=1.02, p<.01), older age (OR=1.324, p<.01), and low positive affect (OR=0.91, p<.01) were each related to an increased likelihood of opioid use. Youth with SCD take opioids appropriately in response to their pain, based on daily self-report. Beyond daily pain severity, age, and daily variation in positive affect were related to home-based opioid use. This suggests that behavioral interventions that enhance positive affect may promote reduced opioid use among youth with SCD.

Resistance of Indonesian tilapia strains, Oreochromis niloticus to Tilapia Lake Virus Disease (TiLVD)

This study aims to evaluate the resistance of six Indonesian tilapia strains to TiLV disease and assess the impact of mimicked daily water temperature fluctuations on the disease severity. Six widely cultured Indonesian tilapia (Oreochromis niloticus) strains were evaluated for resistance to tilapia lake virus disease (TiLVD). Resistance to TiLVD was assessed using two artificial infection methods, cohabitation and intraperitoneal (IP) injection. Fish infected with TiLVD exhibited behavioural, clinical and mortality patterns associated with TiLV disease. Virus presence was also confirmed molecularly. All six tilapia strains were susceptible to TiLV infection, causing disease and mortality. The range of mortality for cohabitation was 1.67% - 28.33% and for IP injection was 16.67% - 61.67%. The most resistant tilapia strain to TiLV disease was Gesit, followed by Best, Red/Albino, Nirwana, Non-specific and Srikandi. Daily temperature variation results in higher significant virus-induced mortality pattern among tilapia strains. These findings can be used to guide the selection of appropriate tilapia strain and temperature control to mitigate the adverse effects of TiLV associated disease.

Investigating Stagnant Air Conditions in Almaty: A WRF Modeling Approach

This study investigates stagnant atmospheric conditions in Almaty, Kazakhstan, a city nestled within a complex terrain. These conditions, characterized by weak local winds and inversion layers, trap pollutants within the city, particularly during winter. The Weather Research &amp; Forecasting (WRF) model was employed to simulate atmospheric conditions using Local Climate Zone data. Verification of the model’s accuracy was achieved through comparisons with data from weather stations and the Landsat-9 satellite. The model successfully reproduced the observed daily temperature variations and weak winds during the testing period (13–23 January 2023). Comparisons with radiosonde data revealed good agreement for morning temperature profiles, while underestimating the complexity of the evening atmospheric structure. The analysis focused on key air quality factors, revealing cyclical patterns of ground-level and elevated inversions linked to mountain-valley circulation. The model effectively captured anabatic and katabatic flows. The study further examined the urban heat island (UHI) using a virtual rural method. The UHI exhibited daily variations in size and temperature, with heat transported by prevailing winds and katabatic flows. Statistical analysis of temperature and wind patterns under unfavorable synoptic situations revealed poor ventilation in Almaty. Data from three Januaries (2022/2023/2024) were used to create maps showing average daytime and nighttime air temperatures, wind speed, and frequency of calm winds.

Analysis of the spectrum of 27-day GCR variations in 2015–2016

This work presents the results of the analysis of 27-day variations in the flux of galactic protons registered by STEREO, SOHO, and AMS-02 in 2015–2016. We calculate variations’ amplitude using several different approaches and propose a description of its energy dependence in the range of magnetic rigidity from approximately 0.3 to 100 GV. We also analyse variations of the solar modulation potential, which is the only parameter of force-field model, and consider the possibility of describing the 27-day variations of galactic cosmic rays within the framework of this model. It is shown that rigidity dependence of the 27-day variations amplitude cannot be properly described using this simple approximation.

Dynamics of Water Use Efficiency of Coniferous and Broad-Leaved Mixed Forest in East China

The aim of our study is to understand the patterns of variation in water use efficiency (WUE) in coniferous and broad-leaved mixed forest ecosystems across multiple scales and to identify its main controlling factors. We employ the eddy covariance method to gather data from 2017, 2018, and 2020, which we use to calculate the gross primary productivity and evapotranspiration of these forests in East China and to determine WUE at the ecosystem level. The mean daily variation in WUE ranges from 4.84 to 7.88 gC kg−1 H2O, with a mean value of 6.12 gC kg−1 H2O. We use ridge regression analysis to ascertain the independent effect of environmental factors on WUE variation. We find that WUE responds differently to environmental factors at different time scales. In mixed conifer ecosystems, temperature and relative humidity emerge as the most significant environmental factors influencing WUE variability. Especially at the seasonal scale, temperature and relative humidity can explain more than 51% of the WUE variation. Our results underscore the varied effects of environmental factors on WUE variation across different time scales and aid in predicting the response of WUE to climate change in coniferous and broad-leaved mixed forest ecosystems.

Reciprocal Coupling of Circadian Clocks in the Compound Eye and Optic Lobe in the Cricket Gryllus bimaculatus.

The circadian system comprises multiple clocks, including central and peripheral clocks. The central clock generally governs peripheral clocks to synchronize circadian rhythms throughout the animal body. However, whether the peripheral clock influences the central clock is unclear. This issue can be addressed through a system comprising a peripheral clock (compound eye clock [CE clock]) and central clock (the optic lobe [OL] clock) in the cricket Gryllus bimaculatus. We previously found that the compound eye regulates the free-running period (τ) and the stability of locomotor rhythms driven by the OL clock, as measured by the daily deviation of τ at 30°C. However, the role of the CE clock in this regulation remains unexplored. In this study, we investigated the importance of the CE clock in this regulation using RNA interference (RNAi) of the period (per) gene localized to the compound eye (perCE-RNAi). The perCE-RNAi abolished the compound eye rhythms of the electroretinogram (ERG) amplitude and clock gene expression but the locomotor rhythm driven by the OL clock was maintained. The locomotor rhythm of the tested crickets showed a significantly longer τ and greater daily variation of τ than those of control crickets treated with dsDsRed2. The variation of τ was comparable with that of crickets with the optic nerve severed. The τ was considerably longer but was comparable with that of crickets with the optic nerve severed. These results suggest that the CE clock regulates the OL clock to maintain and stabilize τ.

Daily geomagnetic variations under variable IMF/solar conditions and their connection with underground conductivity changes in Japan

Fluctuations in Solar Wind (SW) and Interplanetary Magnetic Field (IMF) significantly affect the Geomagnetic Field (GF) measurements particularly during extreme space weather events. The current study investigates the variations of horizontal (H), vertical (Z) geomagnetic components under quiet and disturbed IMF and solar conditions as well as the effect of underground conductivity on the stationary geomagnetic measurements in Japan. Results of data analysis show that the response of the GF to IMF and solar parameters fluctuations is variable. The H- and Z-components were in good harmony with high visual correlation along a latitudinal profile across Japan during quiet times. On the other hand, during the disturbed times related to a Coronal Mass Ejection (CME) launched on 13 May 2005, the GF components varied with the disturbance of IMF and solar parameters. The H-components showed highly correlated variations with a significant reduction along the examined profile due to intensified ionospheric currents, while the Z-components recorded in the northern part of Japan showed abnormal daily variations pattern (positive daily variations) with an enhanced amplitude which is opposite to the normal behavior of daily variations recorded in the central and southern parts of Japan (negative daily variations). The observed enhanced and abnormal daily variation of Z-components in north Japan, which we consider a remarkable observation here, is possibly linked with underground conductivity anomaly in this region.

Feeding behavior modifies the circadian variation in RR and QT intervals by distinct mechanisms in mice.

Rhythmic feeding behavior is critical for regulating phase and amplitude in the ≈24-h variation of heart rate (RR intervals), ventricular repolarization (QT intervals), and core body temperature in mice. We hypothesized changes in cardiac electrophysiology associated with feeding behavior were secondary to changes in core body temperature. Telemetry was used to record electrocardiograms and core body temperature in mice during ad libitum-fed conditions and after inverting normal feeding behavior by restricting food access to the light cycle. Light cycle-restricted feeding modified the phase and amplitude of 24-h rhythms in RR and QT intervals, and core body temperature to realign with the new feeding time. Changes in core body temperature alone could not account for changes in phase and amplitude in the ≈24-h variation of the RR intervals. Heart rate variability analysis and inhibiting β-adrenergic and muscarinic receptors suggested that changes in the phase and amplitude of 24-h rhythms in RR intervals were secondary to changes in autonomic signaling. In contrast, changes in QT intervals closely mirrored changes in core body temperature. Studies at thermoneutrality confirmed that the daily variation in QT interval, but not RR interval, primarily reflected daily changes in core body temperature (even in ad libitum-fed conditions). Correcting the QT interval for differences in core body temperature helped unmask QT interval prolongation after starting light cycle-restricted feeding and in a mouse model of long QT syndrome. We conclude feeding behavior alters autonomic signaling and core body temperature to regulate phase and amplitude in RR and QT intervals, respectively.NEW & NOTEWORTHY We used time-restricted feeding and thermoneutrality to demonstrate that different mechanisms regulate the 24-h rhythms in heart rate and ventricular repolarization. The daily rhythm in heart rate reflects changes in autonomic input, whereas daily rhythms in ventricular repolarization reflect changes in core body temperature. This novel finding has major implications for understanding 24-h rhythms in mouse cardiac electrophysiology, arrhythmia susceptibility in transgenic mouse models, and interpretability of cardiac electrophysiological data acquired in thermoneutrality.

HiPSC-CM electrophysiology: impact of temporal changes and study parameters on experimental reproducibility.

Human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) are frequently used for preclinical cardiotoxicity testing and remain an important tool for confirming model-based predictions of drug effects in accordance with the comprehensive in vitro proarrhythmia assay (CiPA). Despite the considerable benefits hiPSC-CMs provide, concerns surrounding experimental reproducibility have emerged. We investigated the effects of temporal changes and experimental parameters on hiPSC-CM electrophysiology. iCell cardiomyocytes2 were cultured and biosignals were acquired using a microelectrode array (MEA) system (2-14 days). Continuous recordings revealed a 22.6% increase in the beating rate and 7.7% decrease in the field potential duration (FPD) during a 20-min equilibration period. Location-specific differences across a multiwell plate were also observed, with iCell cardiomyocytes2 in the outer rows beating 8.8 beats/min faster than the inner rows. Cardiac endpoints were also impacted by cell culture duration; from 2 to 14 days, the beating rate decreased (-12.7 beats/min), FPD lengthened (+257 ms), and spike amplitude increased (+3.3 mV). Cell culture duration (4-10 days) also impacted cardiomyocyte drug responsiveness (E-4031, nifedipine, isoproterenol). qRT-PCR results suggest that daily variations in cardiac metrics may be linked to the continued maturation of hiPSC-CMs in culture (2-30 days). Daily experiments were also repeated using a second cell line (Cor.4U). Collectively, our study highlights multiple sources of variability to consider and address when performing hiPSC-CM MEA studies. To improve reproducibility and data interpretation, MEA-based studies should establish a standardized protocol and report key experimental conditions (e.g., cell line, culture time, equilibration time, electrical stimulation settings, and raw data values).NEW & NOTEWORTHY We demonstrate that iCell cardiomyocytes2 electrophysiology measurements are impacted by deviations in experimental techniques including electrical stimulation protocols, equilibration time, well-to-well variability, and length of hiPSC-CM culture. Furthermore, our results indicate that hiPSC-CM drug responsiveness changes within the first 2 wk following defrost.

What accounts for the difference in the emergence times of Drosophila melanogaster between the first and second eclosion days?

AbstractOne of the characteristics of the adult eclosion rhythm in Drosophila melanogaster is that adult emergence time differs greatly between the first and second eclosion days. The emergence time is in the middle of the light period on the first eclosion day, but immediately after light‐on on the second day. We hypothesized that incomplete entrainment of the endogenous pacemaker to the light–dark (LD) cycle is responsible for the daily variation. Due to the very short pupal period of this species, adult emergence may occur before complete synchronization of the pacemaker with the external cycle on the first eclosion day. Therefore, the peak time on the first eclosion day may differ significantly from that on the second day. To verify this hypothesis, using pupae that had pupariated within 24 h, the time difference between the first and second peaks was compared in LD 12:12 or constant darkness at five different temperatures from 30 to 15°C. In both light regimes, the time difference decreased with decreasing temperature and extended pupal duration. The interval between the eclosion peaks approached 24 h, supporting this hypothesis. These results can be interpreted by using a two‐oscillator model.

Racial and ethnic minorities disproportionately exposed to extreme daily temperature variation in the United States.

In the history of Homo sapiens, well-populated habitats have featured relatively stable temperatures with generally small daily variations. As the global population is increasingly residing in highly disparate climates, a burgeoning literature has documented the adverse health effects of single-day and day-to-day variation in temperature, raising questions of inequality in exposure to this environmental health risk. Yet, we continue to lack understanding of inequality in exposure to daily temperature variation (DTV) in the highly unequal United States. Using nighttime and daytime land surface temperature data between 2000 and 2017, this study analyzes population exposure to long-term DTV by race and ethnicity, income, and age for the 50 states and the District of Columbia. The analysis is based on population-weighted exposure at the census-tract level. We find that, on average, non-White (especially Black and Hispanic) and low-income Americans are exposed disproportionately to larger DTV. Race-based inequalities in exposure to DTV are larger than income-based disparities, with inequalities heightened in the summer months. In May, for example, the DTV difference by race and ethnicity of 51 states is between 0.20 and 3.01 °C (up to 21.0%). We find that younger populations are, on average, exposed to larger DTV, though the difference is marginal.

The moderating role of conscientiousness in the temporal association of stress on sleep.

Personality traits have been associated with sleep problems and stress experience. However, their impact on objective sleep and the temporal relationship of stress on sleep has remained elusive. This study examined whether daytime stress predicts sleep the following night, and the moderating role of neuroticism and conscientiousness in this relationship. To introduce stress variability in natural daily stressors, we measured college students (N = 92) during exams (e.g. high academic stress) and at the start of new course period (e.g. low academic stress). Both objective (actigraphy) and subjective sleep, and daily self-reported stress, were measured for 14 days and personality traits once. Reported daily stress was significantly higher in the exam period compared with baseline, suggesting that our natural manipulation did indeed result in variation in stress levels. Intra-individual daily variations in stress were not associated with the following night's sleep timing, duration or fragmentation, implying that more stress during the day did not affect sleep the following night. Higher levels of neuroticism were associated with poorer daily subjective sleep quality and higher stress levels over the complete period. Neuroticism did not moderate the temporal association of stress on sleep. Conscientiousness moderated the association between intra-individual stress and sleep fragmentation, and intra-individual stress and wake-up time. This implied that highly conscientious participants experienced less sleep fragmentation and woke-up earlier after more stressful days. These results suggest an interconnected relationship among stress, sleep and personality. Focusing on one aspect, like handling stress or enhancing sleep quality, might yield positive effects on the rest.

Intersatellite Comparisons of GOES Magnetic Field Measurements

AbstractGOES‐16 and GOES‐17 are the first of NOAA's Geostationary Operational Environmental Satellite (GOES)‐R series of satellites. Each GOES‐R satellite has a magnetometer mounted on the end (outboard) and one part‐way down a long boom (inboard). This paper demonstrates the relative accuracy and stability of the measurements on a daily and long‐term basis. The GOES‐16 and GOES‐17 magnetic field observations from 2017 to 2020 have been compared to simultaneous magnetic field observations from each other and from the previous GOES‐NOP series satellites (GOES‐13, GOES‐14 and GOES‐15). These comparisons provide assessments of relative accuracy and stability. We use a field model to facilitate the inter‐satellite comparisons at different longitudes. GOES‐16 inboard and outboard magnetometers data suffer daily variations which cannot be explained by natural phenomena. Long‐term‐averaged GOES‐16 outboard (OB) data has daily variations of ±3 nT from average values with one‐sigma uncertainty of ±1.5 nT. Long‐term averaged GOES‐17OB magnetometer data have minimal daily variations. Daily average of the difference between the GOES‐16 outboard or GOES‐17 outboard measurements and the measurements made by another GOES satellite are computed. The long‐term averaged results show the GOES‐16OB and GOES‐17OB measurements have long‐term stability (±2 nT or less) and match measurements from magnetometers on other GOES within limits stated herein. The GOES‐17OB operational offset (zero field value) was refined using the GOES‐17 satellite rotated 180° about the Earth pointing axis (known as a yaw flip).

Construction alignment and closure control of CFST truss arch bridges based on temperature effect

To reveal the temperature deformation laws and achieve the accurate alignment prediction during the installation process of long-span concrete-filled steel tubular arch bridge ribs in complex environments, this paper establishes a three-dimensional refined heat transfer model for temperature field and effect calculation of truss arch ribs. The model uses the Ray Tracing Technology to recognize the member-caused-shading area on arch ribs. And by comparing with the actual measurement results and shadow effects to achieve the accurate calculation of temperature field. Finally, the variation of ribs deformation during construction and the optimal closure time are detailed analyzed. Results show that the computational method used in this paper can achieve accurate recognition of shadows and precise calculation of the complex three-dimensional temperature field of truss arch ribs. After considering the shadows, the maximum temperature difference between the upper chord sunshine area and lower chord shadow areas can reach 25 °C, which is significantly greater than 8 ℃ in the "Specifications for design of highway concrete-filled steel tubular arch bridge" (JTG/T D65–06-2015). Due to the solar incidence variation along the longitudinal direction of the arch ribs, extreme temperature fluctuation of the chord tubes occurs, with a maximum temperature difference of 21 °C. When arch ribs reach the maximum cantilever, the daily vertical displacement variation can reach approximately 87 mm, and the maximum displacement difference between arch ribs of two canyon sides can reach 15.6 mm at the same time. The solar-caused maximum compressive stress is 34 MPa, which can exceed 100 MPa after superimposing the gravity and cable effects. Based on the analysis of the displacement of both sides of the closure joint under the influence of sunlight, this paper identifies the window period for the closure control of the long-span steel truss arch ribs, laying the foundation for the accurate control of the rib construction alignment and the closure internal force state.

Immune-pineal-ocular axis in amphibians: unveiling a novel connection?

Melatonin is a hormone known as an endogenous temporal marker signaling the dark phase of the day. Although the eyes seem to be the main site of melatonin production in amphibians, little information is available about the natural variation in the ocular melatonin levels and its modulation following immune stimulation. We investigated the daily variation of plasma and ocular melatonin levels in bullfrogs (Lithobates catesbeianus) and their modulation following an immune stimulation with lipopolysaccharide (LPS) in yellow cururu toads (Rhinella icterica). For the daily variation, bullfrogs were bled and then euthanized for eye collection every 3h over 24h to determine plasma and ocular melatonin levels. We found a positive correlation between ocular and plasma melatonin levels, with maximum values at night (22h) for both plasma and the eyes. For immune stimulation, yellow cururu toads received an intraperitoneal injection of LPS or saline solution during the day (10h) or at night (22h). Two hours after injection, toads were bled and euthanized for eye collection to obtain plasma and ocular melatonin levels. In addition, the liver and bone marrow were collected to investigate local melatonin modulation. Our results demonstrate that retina light-controlled rhythmic melatonin production is suppressed while liver and bone marrow melatonin levels increase during the inflammatory assemblage in anurans. Interestingly, the LPS injection decreased only ocular melatonin levels, reinforcing the central role of the eyes (i.e., retina) as an essential organ of melatonin production, and a similar role to the pineal gland during the inflammatory response in amphibians. Together, these results point to a possible immune-pineal-ocular axis in amphibians, yet to be fully described in this group.

Higher accuracy estimation of the weighted mean temperature (Tm) using GPT3 model with new grid coefficients over China

The weighted mean temperature (Tm) is an essential parameter in the field of Global Navigation Satellite System (GNSS) meteorology, as it enables the conversion of zenith wet delay to atmospheric precipitable water vapor (PWV). The existing Tm models, such as the GPT3 model, utilize the empirical annual and semi-annual amplitudes of Tm at grid points along with corresponding trigonometric functions to estimate Tm, making it difficult to provide a more detailed description of the daily variation of Tm. Therefore, the measured surface temperature and water vapor pressure were introduced to calculate the new series of grid coefficients using the least-square method, which enables the GPT3 model to estimate Tm with higher accuracy. In this process, the ERA5 reanalysis data derived from ECMWF from 2001 to 2010 was utilized to compute Tm values, and the new grid coefficient obtained by introducing only surface temperature was considered as scheme #1 and that achieved by introducing both surface temperature and surface water vapor pressure was regarded as scheme #2. The Tm data of 2023 calculated both by the radiosonde and ERA5 reanalysis data were utilized to assess the new grid coefficients for Tm estimation, and the results show that the grid coefficients based on these two surface parameters outperformed those only using the temperature. In the compassion using the ERA5 data, the bias/MAE/RMSE with the two types of new grid coefficients are −0.09/1.89/2.41 K and − 0.07/1.82/2.31 K, respectively, and the improvements reach to 50%/32.5%/34.2% and 61.1%/35%/36.9% compared with GPT3 model. In the comparison with radiosonde data, the scheme #1 and #2 performed a 0.55 K(13.8%) and 0.68 K(17.1%) improvement in the mean RMSE of stations compared with the GPT3 model. Note that the proposed two schemes can effectively improve the accuracy of estimating Tm in the comparison of regions, epochs and seasons with the Bevis and GPT3 model. Furthermore, the impact of Tm on PWV can be improved by the proposed two schemes, which was demonstrated using the theoretical RMSE and relative error of PWV.

Weather‐Induced Satellite Orbit Perturbations

AbstractSatellites in Earth's orbit are exposed to Earth radiation, consisting of reflected solar and emitted thermal radiation, thereby exerting a non‐conservative force that causes acceleration and affects the orbits. Gravity Recovery and Climate Experiment Follow‐On (GRACE‐FO) mission aiming to retrieve the Earth's gravity potential is critically dependent on accounting for this force and all other non‐gravitational forces. There are both diurnal and seasonal variations in the Earth's radiation pressure, of which the seasonal variability can be represented by climatology. Nevertheless, the daily variations in the Earth's radiation pressure, due to the transient changes in the weather; for example, clouds and their properties, are not accounted for in the orbit perturbations studies. We show here that the top‐of‐atmosphere radiation fluxes computed with a numerical weather prediction (NWP) model explain most of the measured short‐term variations in the radial acceleration of the GRACE‐FO satellite. Our physics‐based modeling corrects a hitherto unexplained lack of power spectral density in the measured accelerations. For example, we can accurately model the accelerations associated with a tropical storm in the Indian Ocean in December 2020, which would not be possible when using climatological data. Our results demonstrate that using a global numerical weather prediction model significantly improves the simulation of non‐gravitational effects in the satellites' orbits. In the 7‐day data set, OpenIFS‐simulated acceleration exhibited higher accuracy than climatological‐data‐simulated acceleration (2.5 compared to 2.6 nms−2) and an improved precision (2.6 compared to 3.0 nms−2). This advancement contributes to a more precise orbit determination across various applications in Earth sciences.

Surface Solar Radiation Resource Evaluation of Xizang Region Based on Station Observation and High-Resolution Satellite Dataset

Xizang boasts a vast and geographically complex landscape with an average elevation surpassing 4000 m. Understanding the spatiotemporal distribution of surface solar radiation is indispensable for simulating surface processes, studying climate change, and designing photovoltaic power generation and solar heating systems. A multi-dimensional, long-term, spatial, and temporal investigation of solar radiation in Xizang was conducted using three unique datasets, including the difference in surface solar radiation (SSR) between high-resolution satellite and ground station data, the annual and monthly distribution of SSR, and the interannual–monthly–daily variation and the coefficient of hourly variability. Combined with high-resolution elevation data, a strong linear correlation was shown between the radiation and the elevation below 4000 m. Furthermore, analysis reveals greater differences in data between east and west compared to the center, as well as between summer and winter seasons. SSR levels vary in steps, reaching the highest from Ngari to Shigatse and the lowest in a U-shaped area formed by southeastern Shannan and southern Nyingchi. In June, high monthly SSR coverage was the highest of the year. Since 1960, the annual mean SSR has generally exhibited a declining trend, displaying distinctive trends across various seasons and datasets. Owing to intricate meteorological factors, some regions exhibited double peaks in monthly SSR. Finally, we have introduced a solar resource assessment standard, along with a multidimensional evaluation of the resources, and categorized all townships. We offer a thorough analysis of Xizang’s solar radiation to provide a comprehensive understanding, which will help to prioritize recommendations for PV construction in Xizang.

Effects of Water and Nitrogen Control on the Growth Physiology, Yields, and Economic Benefits of Lycium barbarum Plants in a Lycium barbarum + Alfalfa System.

In the production of economic forests, there are common issues such as excessive application of water and fertilizer, redundant plant growth, and low economic benefits. Reasonable water and fertilizer management can not only help address these problems but also improve the absorption and use efficiency of water and fertilizer resources by plants, promoting the green and efficient development of the fruit and forestry industry. In order to explore a suitable water and nitrogen management mode for Lycium barbarum, field experiments were conducted in this study from 2021 to 2022. Specifically, four irrigation modes (according to the proportion ratio of soil moisture content to field moisture capacity θf, 45-55% θf (W1, severe water deficiency), 55-65% θf (W2, moderate water deficiency), 65-75% θf (W3, mild water deficiency), and 75-85% θf (W4, sufficient irrigation)) and four nitrogen application levels (0 kg·ha-1 (N0, no nitrogen application), 150 kg·ha-1 (N1, low nitrogen application level), 300 kg·ha-1 (N2, medium nitrogen application level), and 450 kg·ha-1 (N3, high nitrogen application level)) were set up to analyze the influences of water and nitrogen control on the plant height, stem diameter, chlorophyll content, photosynthetic characteristics and yield, and economic benefits of Lycium barbarum in the Lycium barbarum + Alfalfa system. The study results show that the plant height and stem diameter increment of Lycium barbarum increase with the irrigation amount, increasing first and then decreasing with the increase in the nitrogen application level. Meanwhile, the chlorophyll contents in Lycium barbarum continuously increase throughout their growth periods, with Lycium barbarum treated with W4N2 during all growth periods presenting the highest contents of chlorophyll. In a Lycium barbarum + Alfalfa system, the daily variation curve of the Lycium barbarum net photosynthetic rate presents a unimodal pattern, with maximum values of the daily average net photosynthetic rate and daily carboxylation rate appearing among W4N2-treated plants (19.56 μmol·m-2·s-1 and 157.06 mmol·m-2·s-1). Meanwhile, the transpiration rates of Lycium barbarum plants continuously decrease with the increased degree of water deficiency and decreased nitrogen application level. W1N2-treated plants exhibit the highest leaf daily average water use efficiency (3.31 μmol·s-1), presenting an increase of 0.50-10.47% in efficiency compared with plants under other treatments. The coupling of water and nitrogen has significantly improved the yields and economic benefits of Lycium barbarum plants, with W4N2-treated and W3N2-treated plants presenting the highest dried fruit yield (2623.07 kg·ha-1) and net income (50,700 CNY·ha-1), respectively. Furthermore, compared with other treatment methods, these two treatment methods (W4N2 and W3N2) exhibit increases of 4.04-84.08% and 3.89-123.35% in dried fruit yield and net income indexes, respectively. Regression analysis shows that, in a Lycium barbarum + Alfalfa system, both high yields and economic benefits of Lycium barbarum plants can be achieved using an irrigation amount of 4367.33-4415.07 m3·ha-1 and a nitrogen application level of 339.80-367.35 kg·ha-1. This study can provide a reference for improving the productivity of Lycium barbarum plants and achieving a rational supply of water and nitrogen in Lyciun barbarum + Alfalfa systems in the Yellow River Irrigation Area of Gansu, China, and other similar ecological areas.

Xylem Hydraulic Conductance Role in Kiwifruit Decline Syndrome Occurrence

Kiwifruit decline syndrome (KiDS) has affected kiwifruit orchards for more than ten years in the Mediterranean area, severely compromising productivity and causing extensive uprooting. The affected plants go through an irreversible and fast wilting process. The problem has not been solved yet, and a single cause has not been identified. In this work, we carried out a survey on ten five-year-old healthy kiwifruit cv. Hayward plants cultivated in an area strongly affected by KiDS and characterised by a rising temperature and vapor pressure deficit (VPD). Five plants were located in a KiDS-affected orchard. Our goal was to assess the hydraulic conductance of asymptomatic plants in a KiDS-affected area where rising climate change stress is underway. Our hypothesis was that a rising temperature and VPD could impair xylem functionality, leading the plants to develop strategies of tolerance, such as vessel narrowing, or stress symptoms, such as cavitation or implosion, inducing a higher risk of KiDS onset. Hydraulic conductance was investigated using a physiological and morphological approach to detect trunk sap flow, trunk growth and daily diameter variations, leaf gas exchanges and temperature, stem water potential, and the root xylem vessel diameter and vulnerability to cavitation. A strong xylem vessel narrowing was observed in all plants, with the highest frequency in the 30–45 µm diameter class, which is an indicator of long-term adaptation to a rising VPD. In some plants, cavitation and implosion were also observed, which are indicative of a short-term stress response; this behaviour was detected in the plants in the KiDS-affected orchard, where a high leaf temperature (&gt;39 °C), low stomatal conductance (&lt;0.20 mol H2O m−2 s−1) and transpiration (&lt;3 mmol H2O m−2 s−1), low stem water potential (&lt;−1 MPa), high vulnerability to cavitation (3.7 μm mm−2), low trunk sap flow and high daily stem diameter variation confirmed the water stress status. The concurrence of climate stress and agronomic management in predisposing conditions favourable to KiDS onset are discussed, evidencing the role of soil preparation, propagation material and previous crop.