Sedentary Death Syndrome (SeDS) is the 2nd greatest, yet preventable threat to public health, resulting in multiple chronic diseases and millions of premature deaths annually. Work-related environmental conditions have been implicated as factors related to declines in physical activity (PA) in the United States and abroad. Staff and faculty in a university setting may experience greater quantity of sitting time at work, which would impair the ability to achieve recommended daily levels of PA. Few studies exist that have evaluated sitting levels among university personnel. PURPOSE: The purpose of this investigation was to explore sitting levels among working members of Biola University. METHODS: Eligible survey respondents (N = 393) were men (n = 154, 44.9 ± 12.8 years of age, 178.7 ± 8.3cm in height, 85.3 ± 15.0kg in weight, and an average body mass index (BMI) of 26.7 ± 4.5kg/m2) and women (n = 239, 40.9 ± 13.1 years of age, 164.9 ± 8.1cm in height, 69.2 ± 15.5kg in weight, and an average BMI of 25.56 ± 6.1kg. kg/m2) who are employees of Biola University. Participants completed the International Physical Activity Questionnaire (IPAQ), using the Survey Monkey® platform. Distinction between faculty and staff positions were made along with job type and educational status. Total sitting min/day on a weekday and on a weekend were gathered and used to calculate total weekly sitting minutes and average sitting min/day. Kruskal-Wallis Independent Samples testing was employed to assess group differences. RESULTS: Weekday, weekly and daily average sitting minutes between staff and faculty were significantly (p < 0.05) different. Women employees’(187.84 min/day) weekday sitting was significantly (p < .014) greater than men’s (137.84 min/day). Average daily sitting was significantly (p < .002) different for level of education attained, with masters (157.07 min/day) and doctorate (165.27 min/day) employees sitting less than those with a 2-yr college degree (247.44 min/day). There were no differences in total minutes of sitting on the weekend between faculty and staff, gender, job type or educational status. CONCLUSIONS: Clear discrepancy in PA exists between staff and faculty, men and women and educational attainment. Thus necessitating targeted interventions for the increase of PA on University campuses.