Daily Oscillations Research Articles

Estimating oxygen consumption of rainbow trout (Oncorhynchus mykiss) in a raceway: A Precision Fish Farming approach

The Precision Fish Farming (PFF) approach was applied to the estimation of fish oxygen consumption of rainbow trout in a raceway farm. A dynamic model, simulating the evolution of Dissolved Oxygen concentration, was identified: the daily oscillation of fish oxygen consumption rate was simulated by means of a sinusoidal function. The model was applied to the data set collected during a four-week field study, which was carried out in July 2019. Water temperature and Dissolved Oxygen concentration were measured with an hourly frequency in farm influent and effluent. Fish biomass was monitored on a daily basis by combining the data provided by a state-of-the art system for non-invasive estimation of fish weight distribution with mortality counting. The monitoring period was partitioned into two time-windows, as fish was not fed during the first two weeks. These windows were further partitioned into a calibration and validation set. Three model parameters, i.e. the average daily respiration rate, the amplitude of its daily oscillation, and its phase were estimated by fitting the model output to the time series of DO concentration in the effluent. The results of the calibration show that: 1) the daily average oxygen consumption rate is consistent with the literature; 2) the amplitude of the daily oscillation when fish is regularly fed is more than twice that estimated for fasting fish. The results of the validation suggest that the model could be used to implement a cost-effective automatic control of oxygen supply, based on the short-term prediction of oxygen demand.

Struktura bimodal'nosti napravleniya pribrezhnogo techeniya u Yuzhnogo berega Kryma v 2002–2008 godakh

This paper aims at improvement of knowledge about the structure of bimodal distribution of coastal current direction repeatability at Cape Kikineiz of the Southern coast of Crimea as per monitoring data obtained at the stationary oceanographic platform of the Black Sea hydrophysical sub-satellite testing area of the MHI RAS. Used for monitoring, a vertical measuring antenna with a fixed aperture of Euler testers of the current horizontal component provided verifiable and synchronic isolation of the current's structural characteristics with a vertical velocity shift and multiscale coherent oscillations of the current. New results of analysis of in situ data are presented regarding characteristics of the coastal current and its variability. The data were obtained within the period from November 2001 to 2008. Change patterns of the long-term coastal current regime are noted. The current's characteristics are matched to the results obtained at Cape Kikineiz of the Southern coast of Crimea for the period from September 2008 to 2019. Regular intense oscillations of the coastal current at the local inertial (17 hrs) and daily component as well as current oscillations with a period of 11.6 day are defined. Evolution is analyzed of probability density distribution of the velocity module and current vector direction after calculations, during which a corresponding vector distribution of intensive inertial-gravitational, mesoscale and synoptic oscillations was excluded in the original vector series of dynamics using a digital filtration method. When excluding the contribution of daily and inertial oscillations, the backward and primary mode ratio in the histograms of the coastal current direction repeatability decreases by more than three times. When excluding the contribution of mesoscale and synoptical oscillations, the backward mode of current is almost absent and there exists only a large-scale coastal current coinciding with a cyclonic direction of the coastal edge of the Black Sea Rim Current. Bimodal distribution of the coastal current direction repeatability is a result of superposition of velocity vectors of a large-scale current and variable contribution of existing orbital velocities of multiscale wave and vortex formations present in the coastal-shelf area.

SynGAP is expressed in the murine suprachiasmatic nucleus and regulates circadian-gated locomotor activity and light-entrainment capacity.

The suprachiasmatic nucleus (SCN) of the hypothalamus functions as the master circadian clock. The phasing of the SCN oscillator is locked to the daily solar cycle, and an intracellular signaling cassette from the small GTPase Ras to the p44/42 mitogen-activated protein kinase (ERK/MAPK) pathway is central to thisentrainmentprocess. Here, we analyzed the expression and function of SynGAP-a GTPase-activating protein that serves as a negative regulator of Ras signaling-within the murine SCN. Using a combination of immunohistochemical and Western blotting approaches, we show that SynGAP is broadly expressed throughout the SCN. In addition, temporal profiling assays revealed that SynGAP expression is regulated over the circadian cycle, with peak expression occurring during the circadian night. Further, time-of-day-gated expression of SynGAP was not observed in clock arrhythmicBMAL1null mice, indicating that the daily oscillation in SynGAP is driven by the inherent circadian timing mechanism. We also show that SynGAP phosphorylation at serine 1138-an event that has been found to modulate its functional efficacy-is regulated by clock time and is responsive to photic input. Finally, circadian phenotypic analysis ofSyngap1heterozygous mice revealed enhanced locomotor activity, increased sensitivity to light-evoked clock entrainment, and elevated levels of light-evoked MAPK activity, which is consistent with the role of SynGAP as a negative regulator of MAPK signaling. These findings reveal that SynGAP functions as a modulator of SCN clock entrainment, an effect that may contribute to sleep and circadian abnormalities observed in patients withSYNGAP1gene mutations.

Serum factor(s) from lung adenocarcinoma patients regulates the molecular clock expression.

Lung cancer is a leading cause of cancer-associated deaths worldwide. Lung cancer may lead to circadian disruption, which could contribute to the development of lung cancer. Recently, several studies using animal models indicated that tumors influence systemic circadian homeostasis in remote tissues. However, it is unclear whether carcinoma of the lungs influences remote circadian rhythm, whether this effect exists in humans, and whether signals from the tumor travel through the blood. In this study, we used a cell-based assay to determine whether serum from patients with lung adenocarcinoma could modulate the molecular clock. We found that the daily oscillation period of Bmal1 was significantly lengthened following treatment with serum from untreated lung adenocarcinoma patients. In addition, heat inactivation of this serum abolished the effect, suggesting that a heat-sensitive circulating factor(s) is present in the serum of untreated lung adenocarcinoma patients. Using real-time PCR, we also examined the mRNA abundance of Bmal1, Cry1, and Per1 in human osteosarcoma u2os cell line, HUVECs and A549 cell lines. The expression of Bmal1 was changed in A549 cells in the presence of sera from lung adenocarcinoma patients. Our study revealed a direct effect of serum from lung adenocarcinoma patients on the molecular clock.

Statistical associations of teleconnection indices and space weather with spring weather pattern in the Eastern Baltic region

AbstractThe studies of associations between solar inputs and climate are mostly designed for winter or cold period; whereas the knowledge about these associations during spring on a day‐to‐day time scale are very scarce. Therefore, the aim of this study is to detect the response of spring air temperature (T), relative humidity (RH), and atmospheric pressure (ATP) to variation in teleconnection indices and space weather variables on the day‐to‐day timescale during the period of 1998–2017 in six cities of Eastern part of the Baltic region. We created a multivariate linear regression model for weather variables including month, the linear and seasonal trend, different teleconnection patterns, El Niño–Southern Oscillation, the Quasi‐biennial Oscillation phase, the presence of Sudden Stratospheric Warming (SSW) and space weather variables. The multivariate models for the mean daily weather variables showed a positive association between T and the daily Arctic oscillation, monthly Scandinavian pattern (SCA) indices, solar proton events (SPEs) with a lag of 1–9 days, and solar wind dynamic pressure (P) with a lag of 1–2 days and negative association between T and East Atlantic/West Russia (EA/WR) index. The mean RH positively correlated with a long‐term and short‐term variation in galactic cosmic rays (GCR) and solar wind speed (SWS) with a lag of 0–6 days and negatively correlated with EAWR and NINO3.4 indices. The mean ATP was negatively associated with both long‐term and short‐term changes in GCR and positively associated with the North Atlantic oscillation, EA/WR and SCA indices, By component of interplanetary magnetic field with a lag of 2 days, P, days of Stream Interaction Regions (SIRs) and SWS with a lag of 4–6 days. These results show the significant short‐term effects of SSW, SPEs, SIRs and solar wind variables on spring weather pattern in the Eastern part of the Baltic region.

The Circadian Clock and Viral Infections.

The circadian clock controls several aspects of mammalian physiology and orchestrates the daily oscillations of biological processes and behavior. Our circadian rhythms are driven by an endogenous central clock in the brain that synchronizes with clocks in peripheral tissues, thereby regulating our immune system and the severity of infections. These rhythms affect the pharmacokinetics and efficacy of therapeutic agents and vaccines. The core circadian regulatory circuits and clock-regulated host pathways provide fertile ground to identify novel antiviral therapies. An increased understanding of the role circadian systems play in regulating virus infection and the host response to the virus will inform our clinical management of these diseases. This review provides an overview of the experimental and clinical evidence reporting on the interplay between the circadian clock and viral infections, highlighting the importance of virus-clock research.

Dissolved oxygen deficits in a shallow eutrophic aquatic ecosystem (fishpond) – Sediment oxygen demand and water column respiration alternately drive the oxygen regime

Biological processes tend to dominate the oxygen regime of productive waters. However, in shallow aquatic ecosystems, it is unclear whether the oxygen regime is driven by oxygen production and consumption in the water column or by sediment oxygen demand (SOD). In managed eutrophic ecosystems, this question is especially important in the context of extreme daily oscillations of dissolved oxygen (DO) that could breach physiological limits of heterotrophic aerobic organisms. High-frequency measurement of DO, temperature, global radiation (Gl.Rad.), and pH in a 0.6 m deep, 22 ha eutrophic fishpond Rod (Czech Republic) shows that the oxygen regime depended on the ecosystem state. Over the clearwater period in the early season, the DO level reflected ecosystem heterotrophy with relatively low daily DO oscillations. However, during the summer phytoplankton bloom, the fishpond was primarily autotrophic with extreme DO fluctuation. During late summer, a collapse of the phytoplankton bloom and an associated shift towards heterotrophy and DO deficit frequently occur. In-situ mesocosm experiments in Rod fishpond were conducted throughout 2018 and 2019 growing seasons, to address the importance of SOD to the oxygen regime. We enclosed the water column in transparent and opaque/dark plastic cylinders open or closed to the sediment. The results show that the proportional contribution of SOD to total respiration decreased from 70 to 90% at low phytoplankton biomass (expressed as Chlorophyll-a (Chl-a) concentration) to approximately 10% at phytoplankton bloom. At night, the difference between the oxygen consumption in the cylinders with or without sediment was statistically significant, when the concentration of Chl-a was <100 μg·L−1. On the contrary, the difference was not significant when the concentration of Chl-a was >100 μg·L−1. This revealed that the impact of SOD is negligible at high phytoplankton biomass.

Diel oscillations in the feeding activity of heterotrophic and mixotrophic nanoplankton in the North Pacific Subtropical Gyre

Daily oscillations in photosynthetically active radiation strongly influence the timing of metabolic processes in picocyanobacteria, but it is less clear how the light-dark cycle affects the activities of their consumers. We investigated the relationship between marine picocyanobacteria and nanoplanktonic consumers throughout the diel cycle to determine whether heterotrophic and mixotrophic protists (algae with phagotrophic ability) display significant periodicity in grazing pressure. Carbon biomass of Prochlorococcus and Synechococcus was estimated continuously from abundances and cell size measurements made by flow cytometry. Picocyanobacterial dynamics were then compared to nanoplankton abundances and ingestion of fluorescently labeled bacteria measured every 4 h during a 4 d survey in the North Pacific Subtropical Gyre. Grazing of the labeled bacteria by heterotrophic nanoplankton was significantly greater at night than during the day. The grazing activity of mixotrophic nanoplankton showed no diel periodicity, suggesting that they may feed continuously, albeit at lower rates than heterotrophic nanoplankton, to alleviate nutrient limitation in this oligotrophic environment. Diel changes in Prochlorococcus biomass indicated that they could support substantial growth of nanoplankton if those grazers are the main source of picocyanobacterial mortality, and that grazers may contribute to temporally stable abundances of picocyanobacteria.

Circadian Genes as Therapeutic Targets in Pancreatic Cancer.

Pancreatic cancer is one of the most lethal cancers worldwide due to its symptoms, early metastasis, and chemoresistance. Thus, the mechanisms contributing to pancreatic cancer progression require further exploration. Circadian rhythms are the daily oscillations of multiple biological processes regulated by an endogenous clock. Several evidences suggest that the circadian clock may play an important role in the cell cycle, cell proliferation and apoptosis. In addition, timing of chemotherapy or radiation treatment can influence the efficacy and toxicity treatment. Here, we revisit the studies on circadian clock as an emerging target for therapy in pancreatic cancer. We highlight those potential circadian genes regulators that are commonly affected in pancreatic cancer according to most recent reports.

The Influence of Melatonin on the Daily 24-h Rhythm of Putative Reference Gene Expression in White Adipose Tissues.

In adipose tissue, the expression of hundreds of genes exhibits circadian oscillation, which may or may not be affected by circulating melatonin levels. Using control and pinealectomized rats, we investigated the daily expression profile of Actb, Hprt-1, B2m, and Rpl37a, genes that are commonly used as reference genes for reverse transcription quantitative polymerase chain reaction (RT-qPCR), in epididymal (EP), retroperitoneal (RP), and subcutaneous (SC) adipose tissues. In control rats, Actb expression presented a daily oscillation in all adipose tissues investigated, Hprt-1 showed 24-h fluctuations in only RP and SC depots, B2m was stable over 24 h for EP and RP but oscillated over 24 h in SC adipose tissue, and Rpl37a presented a daily oscillation in only RP fat. In the absence of melatonin, the rhythmicity of Actb in all adipose depots was abolished, the daily rhythmicity of Hprt-1 and B2m was disrupted in SC fat, the peak expression of Rpl37a and Hprt-1 was delayed, and the amplitude of Rpl37a was reduced in RP adipose tissue. Collectively, our results demonstrate that the expression of putative reference genes displays a daily rhythm influenced by melatonin levels in a manner specific to the adipose depot. Thus, the proper standardization and daily profile expression of reference genes should be performed carefully in temporal studies using RT-qPCR analysis.

Nyctinastic thallus movement in the liverwort Marchantia polymorpha is regulated by a circadian clock

The circadian clock coordinates an organism’s growth, development and physiology with environmental factors. One illuminating example is the rhythmic growth of hypocotyls and cotyledons in Arabidopsis thaliana. Such daily oscillations in leaf position are often referred to as sleep movements or nyctinasty. Here, we report that plantlets of the liverwort Marchantia polymorpha show analogous rhythmic movements of thallus lobes, and that the circadian clock controls this rhythm, with auxin a likely output pathway affecting these movements. The mechanisms of this circadian clock are partly conserved as compared to angiosperms, with homologs to the core clock genes PRR, RVE and TOC1 forming a core transcriptional feedback loop also in M. polymorpha.

Decoupling behavioral and transcriptional responses to color in an eyeless cnidarian

BackgroundAnimals have specific molecular, physiological, and behavioral responses to light that are influenced by wavelength and intensity. Predictable environmental changes – predominantly solar and lunar cycles – drive endogenous daily oscillations by setting internal pacemakers, otherwise known as the circadian clock. Cnidarians have been a focal group to discern the evolution of light responsiveness due to their phylogenetic position as a sister phylum to bilaterians and broad range of light-responsive behaviors and physiology. Marine species that occupy a range of depths will experience different ranges of wavelengths and light intensities, which may result in variable phenotypic responses. Here, we utilize the eyeless sea anemone Nematostella vectensis, an estuarine anemone that typically resides in shallow water habitats, to compare behavioral and molecular responses when exposed to different light conditions.ResultsQuantitative measures of locomotion clearly showed that this species responds to light in the blue and green spectral range with a circadian activity profile, in contrast to a circatidal activity profile in the red spectral range and in constant darkness. Differences in average day/night locomotion was significant in each condition, with overall peak activity during the dark period. Comparative analyses of 96 transcriptomes from individuals sampled every 4 h in each lighting treatment revealed complex differences in gene expression between colors, including in many of the genes likely involved in the cnidarian circadian clock. Transcriptional profiling showed the majority of genes are differentially expressed when comparing mid-day with mid-night, and mostly in red light. Gene expression profiles were largely unique in each color, although animals in blue and green were overall more similar to each other than to red light.ConclusionsTogether, these analyses support the hypothesis that cnidarians are sensitive to red light, and this perception results in a rich transcriptional and divergent behavioral response. Future work determining the specific molecular mechanisms driving the circadian and potential circatidal rhythms measured here would be impactful to connect gene expression variation with behavioral variation in this eyeless species.

Rhythmic expression of the melatonergic biosynthetic pathway and its differential modulation in vitro by LPS and IL10 in bone marrow and spleen

Daily oscillation of the immune system follows the central biological clock outputs control such as melatonin produced by the pineal gland. Despite the literature showing that melatonin is also synthesized by macrophages and T lymphocytes, no information is available regarding the temporal profile of the melatonergic system of immune cells and organs in steady-state. Here, the expression of the enzymes arylalkylamine-N-acetyltransferase (AA-NAT), its phosphorylated form (P-AA-NAT) and acetylserotonin-O-methyltransferase (ASMT) were evaluated in phagocytes and T cells of the bone marrow (BM) and spleen. We also determined how the melatonergic system of these cells is modulated by LPS and the cytokine IL-10. The expression of the melatonergic enzymes showed daily rhythms in BM and spleen cells. Melatonin rhythm in the BM, but not in the spleen, follows P-AA-NAT daily variation. In BM cells, LPS and IL10 induced an increase in melatonin levels associated with the increased expressions of P-AA-NAT and ASMT. In spleen cells, LPS induced an increase in the expression of P-AA-NAT but not of melatonin. Conversely, IL10 induced a significant increase in melatonin production associated with increased AA-NAT/P-AA-NAT expressions. In conclusion, BM and spleen cells present different profiles of circadian production of local melatonin and responses to immune signals.

HnRNP K Supports High-Amplitude D Site-Binding Protein mRNA (Dbp mRNA) Oscillation To Sustain Circadian Rhythms

Circadian gene expression is defined by the gene-specific phase and amplitude of daily oscillations in mRNA and protein levels. D site-binding protein mRNA (Dbp mRNA) shows high-amplitude oscillation; however, the underlying mechanism remains elusive. Here, we demonstrate that heterogeneous nuclear ribonucleoprotein K (hnRNP K) is a key regulator that activates Dbp transcription via the poly(C) motif within its proximal promoter. Biochemical analyses identified hnRNP K as a specific protein that directly associates with the poly(C) motif in vitro Interestingly, we further confirmed the rhythmic binding of endogenous hnRNP K within the Dbp promoter through chromatin immunoprecipitation as well as the cycling expression of hnRNP K. Finally, knockdown of hnRNP K decreased mRNA oscillation in both Dbp and Dbp-dependent clock genes. Taken together, our results show rhythmic protein expression of hnRNP K and provide new insights into its function as a transcriptional amplifier of Dbp.

Evolution of reversal of the lowest low waters in a tidal river network

The lowest low waters usually occur during spring tides in tide-dominated coastal environments. However, they could also occur during neaps, rather than springs, at more landward stations, and this phenomenon isknownas the reversal of the lowest low waters (RLLW). The RLLW greatly depends on subtidal and daily oscillations in the water level, the evolutions of which along a channel are of considerable importance in navigation safety and freshwater intake. Observations collected at an inland hydrological station (Shilong) in the networks of the East River, China, show that the lowest low water level previously occurred during neaps, but now occurs during springs. To elucidate the causes of this evolution, T_TIDE was used to extract constituents from water level records collected over the past fifty years at five stations in this river network system, including astronomical constituents, overtides, and fortnightly tides. The results show that the astronomical constituents were significantly amplified in the upstream stations, while the fortnightly tide Msf decreased slightly in the long term. Physically, channel deepening caused by excessive sand excavation has increased the water depth and reduced the effective drag in the friction term of the momentum balance. The reduced frictional effect, reflected by the residual water level slope, is responsible for the significant increase in the astronomical tidal amplitudes. However, they were not strongly influenced by long-term variations in river runoff and sea-level rise. The amplitude ratio between Msf and S2 was used to analyze the spatiotemporal evolution of RLLW. This ratio at Shilong continuously decreased over time, and the location where the ratio was 1 moved upstream, indicating that the RLLW has traveled farther inland in recent decades. The evolution of the RLLW in the East River network implies that the tidal-dominant zone has enlarged in recent decades.

Experimental and Computational Study of the Implementation of mPCM-Modified Gypsum Boards in a Test Enclosure

The application of phase change materials (PCM) in the thermal envelope of buildings has proven to be an alternative to reduce energy consumption and to improve thermal comfort conditions. The present work evaluates the thermal behavior of gypsum boards modified with a microencapsulated PCM (mPCM) in a cubic test enclosure, considering the climatic conditions of Santiago de Chile in the September–November period of 2017. The design of the test enclosure was performed considering the minimization of parameters that affect the variation of its inner temperature and favoring the heat flow through the gypsum boards. Experimentally, the results reflect the main effects of the implementation of a mPCM, among which the displacement of the maximum heat load and the decrease in the daily oscillation of the internal temperature of the test enclosure (up to 2 °C) stand out. In addition, the mPCM modified gypsum board was thermally characterized to carry out a thermal simulation of the enclosures using EnergyPlus™. The obtained numerical results agree with those obtained experimentally, including the behavior of the transient heat flux through the gypsum boards. Moreover, it was found that considering a null infiltration rate gives place to unrealistic results, suggesting that this parameter should be controlled.

Analysis of water vapor adsorption in soils by means of a lysimeter and numerical modeling

AbstractDaily temperature oscillations can cause adsorption (and desorption) of atmospheric water vapor by soils. The resulting daily fluctuations in the amount of liquid water in the soil can be measured by high‐precision weighing lysimeters. We analyzed the data of a lysimeter in a sandy dune sediment in southern Spain using Codebright, a thermohydraulic numerical model for unsaturated flow that takes into account water, vapor, and heat transport in the soil, as well as soil–atmosphere interactions such as precipitation, evaporation, and solar radiation. The analysis shows that daily temperature oscillations, psychrometrics, and soil water retention can explain the fluctuations of the amount of liquid water in the soil. The retention curve, especially its driest part, is essential for the existence of these fluctuations. The fluctuations could not be reproduced by a model using the van Genuchten retention curve with a constant residual saturation. On the other hand, satisfactory results could be obtained by models using retention curves that at their driest part still show a change of saturation with suction. Moreover, the models suggest within the top few decimeters of the soil a pattern of alternating bands of condensation and evaporation, which follows the daily temperature oscillations that fade out deeper in the soil.

Multidimensional analysis of temporal and layered microclimatic behavior of subtropical climber green walls in summer

Existing studies emphasizing the magnitude of green-wall thermal benefits paid little attention to temporal microclimatic behavior. To bridge the knowledge gap, we employed high-precision sensors to monitor the microclimate of two wirerope-type climber green walls and adjacent control bare walls in humid-subtropical Hong Kong. Sample days were systematically selected to represent the results on sunny, cloudy and rainy weather in summer. This study aimed at: (1) evaluating the magnitude and daily temporal occurrence of maximum temperature, cooling and insolation at different positions of the layered green walls, and (2) analyzing temporal occurrence patterns of maximum values of microclimatic variables. The results demonstrated the paramount importance of insolation on green-wall microclimate. Varied solar inputs under different weather brought a sequence in maximum insolation, temperature and cooling: sunny > cloudy > rainy. Achieving an average cooling of 8.5 °C against bare wall surface exterior on sunny days, climber foliage acted as a heat sink for the street canyon and the building shell. The posterior air gap (PAG) generated an effective insulation layer with average cooling of 2.5 °C against ambient air in sunny conditions. However, ambient air anterior to the green wall experienced minor warming up to 0.05 °C. Cooling was stronger on more solar-exposed aspect and under stronger insolation. Green walls steered humidification effect by 0.17 kPa on average on sunny days. Daily temperature oscillation was moderated most obviously at the building exterior with average 4.3 °C reduction in sunny conditions. Implications were drawn on climber-species selection and PAG provision to enhance the multi-dimensional thermal benefits of climber green wall.

Biomarker Profiling of Microbial Mats in the Geothermal Band of Cerro Caliente, Deception Island (Antarctica): Life at the Edge of Heat and Cold.

Substrate–atmosphere interfaces in Antarctic geothermal environments are hot–cold regions that constitute thin habitable niches for microorganisms with possible counterparts in ancient Mars. Cerro Caliente hill in Deception Island (active volcano in the South Shetland Islands) is affected by ascending hydrothermal fluids that form a band of warm substrates buffered by low air temperatures. We investigated the influence of temperature on the community structure and metabolism of three microbial mats collected along the geothermal band of Cerro Caliente registering 88°C, 8°C, and 2°C at the time of collection. High-throughput sequencing of small subunit ribosomal ribonucleic acid (SSU rRNA) genes and Life Detector Chip (LDChip) microarray immunoassays revealed different bacterial, archaeal, and eukaryotic composition in the three mats. The mat at 88°C showed the less diverse microbial community and a higher proportion of thermophiles (e.g., Thermales). In contrast, microbial communities in the mats at 2°C and 8°C showed relatively higher diversity and higher proportion of psychrophiles (e.g., Flavobacteriales). Despite this overall association, similar microbial structures at the phylum level (particularly the presence of Cyanobacteria) and certain hot- and cold-tolerant microorganisms were identified in the three mats. Daily thermal oscillations recorded in the substrate over the year (4.5–76°C) may explain the coexistence of microbial fingerprints with different thermal tolerances. Stable isotope composition also revealed metabolic differences among the microbial mats. Carbon isotopic ratios suggested the Calvin–Benson–Bassham cycle as the major pathway for carbon dioxide fixation in the mats at 2°C and 8°C, and the reductive tricarboxylic acid cycle and/or the 3-hydroxypropionate bicycle for the mat at 88°C, indicating different metabolisms as a function of the prevailing temperature of each mat. The comprehensive biomarker profile on the three microbial mats from Cerro Caliente contributes to unravel the diversity, composition, and metabolism in geothermal polar sites and highlights the relevance of geothermal-cold environments to create habitable niches with interest in other planetary environments.

Daily thermal preference variation of the sand recluse spider Sicarius thomisoides (Araneae: Sicariidae).

Preferential temperature as a physiological feature is crucial for spiders, since it determines the selection of key habitats for their survival and reproduction. In this work, we study the daily and geographical variation of the preferential temperature of the spider Sicarius thomisoides subjected to different degrees of daily thermal oscillation in their habitats. Preferred temperatures differ between coastal and inland populations, but in both cases, there is a marked bimodality in the daily pattern of temperature preference, with two peaks per day that would be given by the changes in the hours of activity. These nocturnal spiders select higher temperatures in the evening (active period) and select lower temperatures during late morning (resting period). In laboratory, spiders have preferred temperatures that differ from those found in their habitats, so they must tolerate or compensate non-preferred temperatures by active thermoregulation in natural conditions.