Daily Food Intake Research Articles

Organotins in fish, shrimp, and cephalopods from the Pearl River Estuary, China: Dietary exposure risk to Indo-Pacific humpback dolphin and human

Food has regularly been proven to be a key source of exposure to environmental pollutants, drawing attention to the dietary exposure risks of contaminants to mammals with significant daily food intake. Here, the levels of six organotin compounds (OTs) in 18 fish (n = 310), three cephalopods (n = 50), and one shrimp (n = 34) from the Lingdingyang (LDY) and west four region (WFR) of the Pearl River Estuary (PRE) and their dietary exposure risks to Indo-Pacific humpback dolphins and humans were first investigated. Total OT levels ranged from 3.84 to 901. 48 ng/g wet weight (ww) in 22 prey species from the LDY, and from 14.37 to 1364.64 ng/g ww in 19 species from the WFR. The LDY marine species generally accumulated higher butyltin levels but lower phentyltin levels than those in the WFR. All species have a phenyltin degradation index <1 and over 60 % of the sampled species have a butyltin degradation index <1, suggesting the PRE marine species might be exposed to the fresh discharge of OTs. A total of nine marine species exceeded the threshold levels of OT intake for adverse health effects on human juveniles by consumption, all 22 marine species posed high dietary risks to the PRE humpback dolphins. Moreover, probabilistic risk assessment using Monte Carlo simulation revealed that the probabilities of RQ values associated with WFR OT exposure higher than 1 were 18.87 % for human adults, 40.55 % for human juveniles, 100 % for both humpback dolphin adults and humpback dolphin juveniles. Our results highlighted the potentially high dietary exposure risks of OTs to marine mammals and residents in the PRE.

Impact of active lifestyle on the primary school children saliva microbiota composition.

Male (114) and female children (8-10 years) belonging to five primary schools in the neighborhoods of Turin were classified as active (A) or sedentary (S) based on PAQ-C-It questionnaire. PCR amplification of salivary DNA targeted the hypervariable V3-V4 regions of the 16S rRNA bacterial genes. DADA2 workflow was used to infer the Amplicon Sequence Variants and the taxonomic assignments; the beta-diversity was obtained by PCoA with the UniFrac method; LEfSe algorithm, threshold at 5%, and Log LDA cutoff at ±0.5 were used to identify differently abundant species in A compared to S saliva sample. Daily food intake was assessed by 3-Days food record. The metabolic potential of microbial communities was assessed by PICRUSt. No significant differences were found in individual's gender distribution (p = 0.411), anthropometry, BMI (p > 0.05), and all diet composition between A and S groups (p > 0.05). Eight species were differently abundant: Prevotella nigrescens (LDA score = -3.76; FDR = 1.5×10-03), Collinsella aerofaciens (LDA score = -3.17; FDR = 7.45×10-03), Simonsiella muelleri (LDA score = -2.96; FDR = 2.76×10-05), Parabacteroides merdae (LDA score = -2.43; FDR = 1.3×10-02) are enriched in the A group; Gemella parahaemolysans, Prevotella aurantiaca (LDA score = -3.9; FDR = 5.27×10-04), Prevotella pallens (LDA score = 4.23; FDR = 1.93×10-02), Neisseria mucosa (LDA score = 4.43; FDR = 1.31×10-02; LDA score = 2.94; FDR = 7.45×10-03) are enriched in the S group. A prevalence of superpathway of fatty acid biosynthesis initiation (E. coli) and catechol degradation II (meta-cleavage pathway) was found in saliva from A compared to S children. Our results showed that active children had an enrichment of species and genera mainly associated with a healthier profile. By contrast, the genera and the species enriched in the sedentary group could be linked to human diseases.

Fasting - a potent modern therapy

Therapeutic fasting has found its way into modern medicine in the last decade through a multitude of experimental work and animal studies as well as increasing clinical research. It is a procedure with a tradition dating back thousands of years and thus comes with a variety of different practices. What they all have in common, is the reduction of daily food intake for a limited period of time. This has a variety of effects on metabolism, cells and organ systems, which can make it a potent tool in medical practice.

Hubungan riwayat sarapan pagi, tingkat kecukupan gizi makro dan status gizi dengan prestasi belajar anak di SD Inpres Lili Kabupaten Kupang

Breakfast is a very important mealtime, representing 15-25% of the total daily energy intake or 15-30% of the total daily nutrient intake to achieve a healthy, active, and intelligent life. Nutritional status has a significant influence on the level of intelligence and understanding of lessons in the classroom; if a child's nutritional status is insufficient or even poor, it affects the incompleteness of subject understanding and weakens learning outcomes. The purpose of this research is to analyze the history of breakfast, adequacy of macronutrients, and nutritional status with the academic achievement of children at Inpres Lili Elementary School, Kupang Regency. This type of research is an analytical observational study using a cross-sectional approach. The research sample was determined using purposive sampling method, which consisted of 137 students from Inpres Lili Elementary School, Kupang Regency. Statistical tests used were Chi-Square test and multiple logistic regression. Bivariate analysis results show a significant relationship between breakfast (p=0.000), level of macronutrient adequacy (p=0.000), and nutritional status (p=0.000) with academic achievement. Multivariate analysis results show that the history of breakfast, adequacy of macronutrients, and nutritional status are significantly related to academic achievement. Therefore, schools should implement routine nutritional and health monitoring programs and provide counseling or training on balanced nutritious food consumption.

Population food intake clusters and cardiovascular disease incidence: a Bayesian quantifying of a prospective population-based cohort study in a low and middle-income country.

This study was designed to explore the relationship between cardiovascular disease incidence and population clusters, which were established based on daily food intake. The current study examined 5,396 Iranian adults (2,627 males and 2,769 females) aged 35 years and older, who participated in a 10-year longitudinal population-based study that began in 2001. The frequency of food group consumption over the preceding year (daily, weekly, or monthly) was assessed using a 49-item qualitative food frequency questionnaire (FFQ) administered via a face-to-face interview conducted by an expert dietitian. Participants were clustered based on their dietary intake by applying the semi-parametric Bayesian approach of the Dirichlet Process. In this approach, individuals with the same multivariate distribution based on dietary intake were assigned to the same cluster. The association between the extracted population clusters and the incidence of cardiovascular diseases was examined using Cox proportional hazard models. In the 10-year follow-up, 741 participants (401 men and 340 women) were diagnosed with cardiovascular diseases. Individuals were categorized into three primary dietary clusters: healthy, unhealthy, and mixed. After adjusting for potential confounders, subjects in the unhealthy cluster exhibited a higher risk for cardiovascular diseases [Hazard Ratio (HR): 2.059; 95% CI: 1.013, 4.184] compared to those in the healthy cluster. In the unadjusted model, individuals in the mixed cluster demonstrated a higher risk for cardiovascular disease than those in the healthy cluster (HR: 1.515; 95% CI: 1.097, 2.092). However, this association was attenuated after adjusting for potential confounders (HR: 1.145; 95% CI: 0.769, 1.706). The results have shown that individuals within an unhealthy cluster have a risk that is twice as high for the incidence of cardiovascular diseases. However, these associations need to be confirmed through further prospective investigations.

Climate Change and Water Scarcity at the Focus of Environmental Impacts Associated with the COVID-19 Crisis in Spain

The conditions of social distancing and home confinement imposed to contain the outbreak of the severe acute respiratory syndrome coronavirus 2 (COVID-19) pandemic have changed the dietary patterns and lifestyle of society. These changes have had significant consequences not only for our personal well-being but also for the health of the planet. In this context, the aim of this study was to describe the effect of household containment during the COVID-19 pandemic on greenhouse gas (GHG) emissions related to food and water consumption. In addition, the dietary pattern of the Spanish population during home confinement was compared with the usual dietary pattern of consumption for the immediately preceding reference year (2019) to elucidate in depth the environmental consequences derived from food consumption due to COVID-19 restrictions. Life Cycle Assessment (LCA) and Water Footprint Assessment (WFA) methodologies were used to assess carbon footprint and water footprint. The functional unit of reference was the average daily food intake per capita. The results showed that COVID-19 home confinement had a negative impact on the carbon footprint (CF) and water footprint (WF) of the Spanish dietary pattern mainly due to the increase in the amount of food consumed during 2020. The monthly analysis revealed two peaks in April and October for both environmental indicators in 2020 compared to the same period in 2019, which was mainly due to the increase in food consumption in Spanish households during the weeks of confinement as well as to the new COVID-19 wave in October. On the other hand, an upward trend in GHG emissions and water consumption could be observed in the last months of both years, which is attributed to increased consumption during the Christmas holidays. These findings indicate that people should be more aware than ever of the importance of maintaining a healthy lifestyle and dietary pattern, in line with health guidelines and the Sustainable Development Goals.

The association between different types of dietary fat intake and blood lipids in Type 2 diabetes patients: sex differences

Purpose Blood lipid and lipoprotein abnormalities are common among patients with diabetes. The study aimed to assess dietary fat intake and its association with blood lipids among patients with Type 2 diabetes mellitus (T2DM) considering sex differences.Design/methodology/approach A cross-sectional observational study was conducted with patients (207 males and 197 females) with T2DM. The daily food intake and its contents of fat and fat types were assessed through face-to-face interview. Anthropometric measurements, glycated hemoglobin (HbA1c), triglyceride, total cholesterol (TC), high-density lipoprotein (HDL) cholesterol and low-density lipoprotein (LDL) cholesterol were initially recorded.Findings The results revealed that TC, LDL and HDL cholesterol levels were significantly higher in females than in males. However, the TC: HDL ratio was significantly higher in males than in females. The results also showed that the daily intake of saturated fatty acid (SFA) slightly exceeded the daily recommended allowance. However, the monounsaturated fatty acid + polyunsaturated fatty acid/SFA (MUFA + PUFA/SFA) ratio was within the recommended ratio. In addition, this study found that the main sources of SFA and cholesterol intake were milk and milk products. A significant association between high fat intake and HbA1c levels was observed (r = 0.234, p &lt; 0.001).Research limitations/implications As it is a cross-sectional observational study, this study has the natural limitation where it can only demonstrate an association.Originality/value The types of dietary fat intake may contribute to blood lipid abnormalities and differences effects may exist among male and female. Studies on the effect of daily fat intake and its types on blood lipids in patients with diabetes, in particular Saudi patients with diabetes are limited. This study focused on the amount and type of the consumed fat among male and female Saudi patients with T2DM and studied the relationship between the type of consumed fat and blood lipid profiles.

Xiao-Ban-Xia decoction mitigates cisplatin-induced emesis via restoring PINK1/Parkin mediated mitophagy deficiency in a rat pica model

Ethnopharmacological relevanceAs a traditional Chinese anti-emetic formula, Xiao-Ban-Xia decoction (XBXD) was recorded in Golden Chamber, and has promising anti-emetic effect on chemotherapy-induced nausea and vomiting (CINV). Aim of the studyThis study aimed to determine whether the underlying mechanism of XBXD against CINV is correlated to the restoration of cisplatin-induced PINK1/Parkin mediated mitophagy deficiency and mitigation of gastrointestinal inflammation. Materials and methodsThe rat pica model was established by intraperitoneal injection of cisplatin 6 mg/kg. The daily kaolin consumption, food intake and body weight were recorded every 24 h. The pathological damage of gastric antrum and ileum were observed by hematoxylin-eosin staining. The levels of serum reactive oxygen species (ROS), interleukin-1β (IL-1β) and interleukin-1β (IL-18) were detected by ELISA. The expression of microtubule-associated protein 1 light chain 3 (LC3) in gastric antrum and ileum was detected by Immunofluorescence staining. The levels of LC3II, P62/SQSTM1, PTEN-induced putative protein kinases (PINK1), E3 ubiquitin ligase (Parkin), AMP-dependent protein kinases (AMPK), phosphorylated AMPK (p-AMPK), nuclear factor erythroid 2-related factor (Nrf2) and kelch like ECH Associated Protein 1 (Keap1) in gastric antrum and ileum were assayed by western blotting. ResultsAt 24 h and 72 h following cisplatin challenge, XBXD inhibited cisplatin-induced elevation of kaolin consumption, and improved the daily food intake and body weight loss in rats. Cisplatin-induced gastrointestinal histopathological damages were alleviated, and serum levels of ROS, IL-1β and IL-18 increases were mitigated following XBXD treatments. In gastric antrum and ileum, XBXD activated AMPK-Nrf2 signaling pathway and restored cisplatin-induced PINK1/Parkin mediated mitophagy deficiency. ConclusionsXBXD significantly ameliorated CINV in a cisplatin-induced rat pica model. The underlying anti-emetic mechanism of XBXD might be related to the activation of AMPK-Nrf2 signaling pathway and the restoration of cisplatin-induced PINK1/Parkin-mediated mitophagy deficiency in the gastrointestinal tract.

ANACARDIUM OCCIDENTALE METHANOLIC NUT EXTRACT ATTENUATED TESTICULAR DYSFUNCTIONS IN HIGH-FAT DIET AND STREPTOZOTOCIN-INDUCED DIABETIC MALE WISTAR RATS

Background: Diabetes induces reproductive ailment and modern therapy is still unsatisfactory to combat diabetes-related reproductive impairment. This study investigated effect of Anacardium occidentale nut methanolic extract on testicular dysfunctions in high-fat diet (HFD)/streptozotocin-induced diabetic rats.&#x0D; Materials and Methods: Forty adult male Wistar rats weighing (180 ± 20 g) were used for the experiment. Diabetes was induced with a repeated single dose of freshly prepared streptozotocin (35 mg/kgb.wt) injected intraperitoneally after fed with HFD for six weeks. The animals were randomly allotted into five groups, 8rats/group. Group I: control; Group II: diabetic control; Group III &amp; IV: diabetic rats + low dose (100 mg/kgb.wt) and high dose (200 mg/kgb.wt) Anacardium occidentale nut methanolic extract; Group V: diabetic rats + 200 mg/kgbwp.o metformin for 28 days. Daily food and water intake with weekly body weight and fasting blood glucose were recorded throughout the experimental phase. On the last day of the experiment, the animals were sacrificed, blood sample were collected and testes was harvested for biochemical assay.&#x0D; Results: Reproductive hormones, sperm parameters, testicular antioxidants, testicular B-cell lymphoma-2, testicular enzymes, body and weight of reproductive organs were significant (p&lt;0.05) reduced in diabetic rats with significant (p&lt;0.05) increase in blood glucose, insulin, sperm abnormalities, testicular caspase-3, testicular malondialdehyde (MDA), and food intake. Low dose (100 mg/kgb.wt) and high dose (200 mg/kgb.wt) Anacardium occidentale nut methanolic extract administration significantly (p&lt;0.05) improved the testicular biochemical changes.&#x0D; Conclusion: Anacardium occidentale nut attenuated testicular dysfunctions and could be a novel therapy for diabetes-induced reproductive dysfunctions.&#x0D;

Lactobacillus salivarius and Lactobacillus gasseri supplementation reduces stress-induced sugar craving in mice.

Increased intake of sweets or sugar craving may occur in response to chronic stress representing a risk factor for development of eating disorders and obesity. However, no safe treatment of stress-induced sugar craving is available. In this study we analysed effects of two Lactobacillus strains on food and sucrose intake in mice before and during their exposure to a chronic mild stress (CMS). C57Bl6 mice were gavaged daily for 27days with a mix of L. salivarius (LS) LS7892 and L. gasseri (LG) LG6410 strains or with 0.9% NaCl as a control. Following 10days of gavage, mice were individually placed into the Modular Phenotypic cages, and after 7days of acclimation were exposed to a CMS model for 10days. Food, water and 2% sucrose intakes as well as meal pattern were monitored. Anxiety and depressive-like behaviour were analysed by standard tests. Exposure of mice to CMS was accompanied by increased size of sucrose intake in the control group likely reflecting the stress-induced sugar craving. A consistent, about 20% lower total sucrose intake, was observed in the Lactobacilli-treated group during stress which was mainly due to a reduced number of intakes. Lactobacilli treatment also modified the meal pattern before and during the CMS, showing a decrease of meal number and an increase of meal size with a tendency of reduced total daily food intake. Mild anti-depressive behavioural effects of the Lactobacilli mix were also present. Supplementation of mice with LS LS7892 and LG LG6410 decreases sugar consumption suggesting a potential utility of these strains against stress-induced sugar craving.

The Breakfast Habits Related with Nutritional Status of Students in State Madrasah Ibtidaiyah 1 Kuantan Singingi

Fulfillment of nutritional needs in the morning can be obtained from breakfast, there was by fulfilling 15-30% of daily nutrient intake. Currently, there are still many Indonesian children who still need to consume breakfast under balanced nutrition recommendations. Children who rarely had breakfast are at risk of nutrient deficiencies, affecting their nutritional status. The study aimed to determine the correlation of breakfast habits to the nutritional status of students in State Madrasah Ibtidaiyah (MIN) 1 Kuantan Singingi. This research was conducted in MIN 1 Kuantan Singingi on February 2023. This research method used a cross-sectional design and a total sampling technique. Variabels in this research included breakfast obtained by breakfast recall questionnaire interviews and nutritional status determined based on measurements of the z-socre of BMI/Age. The results showed that 47,5% respondents were normal category and about 57.5% respondents had good breakfast habits. Based on the Chi-Square test results obtained a value of p=0.7460.05, it can be concluded that there was no significant correlation between breakfast to the nutritional status of students in MIN 1 Kuantan Singingi.

Greenhouse Gas Emission and Water Footprint of the National Diet in Turkey: Results from Turkey Nutrition and Health Survey 2017

The study aimed to assess and characterize the sustainability of the national diet in Turkey and its association with diet quality, dietary requirements, and sociodemographic factors. Dietary intake was assessed using 24 h recalls from the Turkey Nutrition and Health Survey 2017 (TNHS-2017) (n = 12,527). The environmental footprints were assessed with two environmental indicators: greenhouse gas emissions (GHGEs) and water footprint (WF). Diet quality was assessed using the diet quality index (DQI) and dietary diversity score (DDS). The dietary GHGEs was 3.21 ± 2.07 kg CO2-eq/person/day and the dietary total WF was 2832 ± 1378 L/person/day. The DQI and DDS were 62.26 ± 8.28 and 6.66 ± 1.31, respectively. Total energy intake was significantly higher in the highest GHGEs and total WF tertiles (2238 ± 722 and 2383 ± 701 kcal, respectively) compared to lower GHGEs and total WF tertiles. Individuals with higher diet-related GHGEs and total WF had a higher daily intake of nutrients with the exception of the percentages of energy supplied from carbohydrates, percentages of meeting nutrients according to the recommended dietary allowance (RDA), DQI (excluding DQI total, moderation, and overall balance score), and DDS scores (p &lt; 0.001). GHGEs and total WF value of the national diet in Turkey are lower than the world average. The results would help develop dietary guidelines to encourage sustainable dietary choices.

Nutrient Composition, Physicobiochemical Analyses, Oxidative Stability and Antinutritional Assessment of Abundant Tropical Seaweeds from the Arabian Sea.

Foods enriched with nutritional compounds and biological activities, especially antioxidants, are considered healthier for human and/or animal consumption. Seaweeds are rich sources of biologically active metabolites and are used as functional foods. In this study, proximate compositions, physicobiochemical characteristics and oil oxidative stability were analyzed for 15 abundant tropical seaweeds (four green-Acrosiphonia orientalis, Caulerpa scalpelliformis, Ulva fasciata, Ulva lactuca; six brown-Iyengaria stellata, Lobophora variegate, Padina boergesenii, Sargassum linearifolium, Spatoglossum asperum, Stoechospermum marginatum; and five red-Amphiroa anceps, Grateloupia indica, Halymenia porphyriformis, Scinaia carnosa, Solieria chordalis). All seaweeds were analyzed for the proximate composition, including moisture content, ash content, total sugar content, total proteins, total lipids, crude fiber, carotenoid content, total chlorophyll content, proline, iodine content, nitrogen-free extract, total phenolic content and total flavonoid content. Green seaweeds showed higher nutritional proximate composition, followed by brown and red seaweeds. Among the different seaweeds, Ulva, Caulerpa, Sargassum, Spatoglossum and Amphiroa showed high nutritional proximate composition compared to other seaweeds. High cation scavenging, free radical scavenging and total reducing activities were observed for Acrosiphonia, Caulerpa, Ulva, Sargassum, Spatoglossum and Iyengaria. It was also observed that 15 tropical seaweeds contained negligible amounts of antinutritional compounds, including tannic acid, phytic acid, saponins, alkaloids and terpenoids. Nutritionally, green and brown seaweeds provided higher sources of energy (150-300 calories per 100 g) compared to red seaweeds (80-165 calories per 100 g). Additionally, this study also confirmed that tropical seaweeds improved the oxidative stability of food oils and, therefore, might be recommended as natural antioxidant additives. The overall results confirm that tropical seaweeds are potential sources of nutrition and antioxidants and may be explored as functional food, dietary supplementation or animal feed. Additionally, they may also be explored as food supplements for fortifying food products, as food toppings or for garnishing and seasoning foods. However, a human or animal toxicity analysis is required before any conclusive recommendation for daily food or feed intake can be made.

THE ASSOCIATION BETWEEN COLORECTAL CANCER AND INDEX OF NUTRITIONAL QUALITY (INQ); A CASE-CONTROL STUDY.

•Is the Index of Nutritional Quality (INQ) associated with colon cancer? •This study compared the INQ of various dietary components between colorectal cancer patients and healthy controls. A total of 480 participants were enrolled in the study (160 patients with colorectal cancer as a case group and 320 healthy control). The results showed that CRC is significantly associated with INQ for some micronutrients. INQ can be considered as an indicator to assess clinical nutritional problems. Background - The nutritional quality of diet may influence the risk of colorectal cancer (CRC). This study compared the Index of Nutritional Quality (INQ) of various dietary components between colorectal cancer patients and healthy controls. Methods - A total of 480 participants were enrolled in the study (160 patients with colorectal cancer as a case group and 320 healthy control). An analysis was conducted on the general characteristics of the participants, their medical histories, anthropometric indicators, physical activity, alcohol consumption, reproductive history, smoking and food intake. A valid food frequency questionnaire was used to assess nutrient intake and INQ was calculated from daily nutrient intake. Results - A Significant inverse association was found between CRC and INQ for vitamins A (OR=0.01, CI: 0.01-0.01), K (OR=0.04, CI: 0.01-0.15), and B12 (OR=0.71, CI: 0.51-0.98), B5 (OR=0.43, CI: 0.00-0.01), zinc (OR=0.35, CI: 0.13-0.95), and phosphorus (OR=0.17, 0.19-0.94). The association between the INQ of vitamin B12 and zinc with colorectal cancer was disappeared after age adjustment. There was a significant negative association between CRC with the INQ of vitamins A, K, B5, phosphorus, and calcium after further adjustments for gender, BMI, menopausal status, and total energy intake. Conclusion -CRC is significantly associated with INQ for some micronutrients. INQ can be considered as an indicator to assess clinical nutritional problems.

Risk of MGUS, a multiple myeloma precursor, in relation to food and beverage intake in the National Health and Nutrition Examination Survey (NHANES).

e20062 Background: Monoclonal gammopathy of undetermined significance (MGUS) is a plasma cell disorder (PCD) with a prevalence of 3% in adults over age 50. The annual risk of progression to multiple myeloma (MM) is 1%. Although one cohort study in Caucasians reported that diets low in certain plant-based foods were associated with MGUS (Thordardottir et al. 2018), research on diet and MGUS in a more diverse population is scarce. Given that diet can vary greatly across ethnic/racial groups, we conducted a population-based study with multiple races and ethnicities. Methods: Using the National Health and Nutrition Examination Survey (NHANES), we conducted a case-control study in 373 individuals with MGUS and 1,406 controls frequency matched on age, body mass index, NHANES cycle, sex, and race. Diet was characterized by one 24-hour dietary recall, with gram intake of individual foods and beverages aggregated into groups. MGUS status was determined by blood screening. Unconditional multivariable logistic regressions were used to model associations between MGUS and intake, with odds ratios and 95% confidence intervals reported for the highest quantile of intake. Results: Daily intake of several food and beverage groups were significantly associated with MGUS, low-risk MGUS (LRMGUS) or intermediate/high-risk MGUS (IHRMGUS). Whole-grain bread, oats, and rice; fruits and vegetables; tomatoes; and cruciferous vegetables were associated with significantly lower risk (28-78%). Sugar- and artificially sweetened beverages were associated with significantly higher risk (34-113%). Interestingly, there were more significant findings for LRMGUS than IHRMGUS, suggesting potential contributing etiologic differences for LRMGUS and IHRMGUS. Conclusions: Our study shows that low intake of whole grains, fruits and vegetables and high intake of sweetened beverages are associated with MGUS. The identification of dietary risk factors for MGUS in a diverse population is of direct public health relevance. These findings also provide rationale for exploration of mechanistic links between diet and PCDs, such as the ongoing studies NCT04920084, NCT05640843, and NCT05312255. [Table: see text]

MATERNAL FOOD RESTRICTION DURING LACTATION PROMOTES RENAL DYSFUNCTION IN THE MALE OFFSPRING AT ADULTHOOD

The aim of this work is to evaluate the renal function and the morphology of the kidneys inthe offspring, of both sexes, of mothers who underwent a 50% food restriction duringlactation. Pregnant Wistar rats were divided into two experimental groups: Control group(CO) fed with standard diet and Food restriction (FR) fed with standard diet restricted to 50%of the daily food intake of CO mothers. The intervention was carried out from the 1st to the14th day of lactation, after from the 14th to the 21st day of lactation. The offspring of bothgroups were fed with standard diet and water ad libitum until 120-day-old. At the end of theexperimental period 24-hour urine collection was performed. After euthanasia biochemicaland histological analysis were performed. Male and female FR offspring showed no change inbody weight or kidney weight compared to control counterparts. Male FR showed a reductionin the glomerular filtration rate (CO 3.22±0.35 vs FR 2.25±0.12; ml/min) and increased totalurinary protein (CO 82.82±11.79 vs FR 123.8±8.37; mg/dL) compared to the CO offspring.We observed a reduction in glomerular area in the male FR (CO 7259±271.7 vs FR6349±106.3; μm 2 ) in comparison to CO offspring. In conclusion, maternal food restrictioncauses functional and morphological kidney damage in adult males, being a sex-specificresponse since females did not show alterations.

Dietary Berberine and Ellagic Acid Supplementation Improve Growth Performance and Intestinal Damage by Regulating the Structural Function of Gut Microbiota and SCFAs in Weaned Piglets

Early weaning is an effective method for improving the utilization rate of sows in intensive pig farms. However, weaning stress induces diarrhea and intestinal damage in piglets. Berberine (BBR) is known for its anti-diarrhea properties and ellagic acid (EA) is known for its antioxidant properties, however, whether their combination improves diarrhea and intestinal damage in piglets has not been studied, and the mechanism remains unclear. To explore the combined effects in this experiment, a total of 63 weaned piglets (Landrace × Yorkshire) were divided into three groups at 21 days. Piglets in the Ctrl group were treated with a basal diet and 2 mL saline orally, while those in the BE group were treated with a basal diet supplemented with 10 mg/kg (BW) BBR, 10 mg/kg (BW) EA, and 2 mL saline orally. Piglets in the FBE group were treated with a basal diet and 2 mL fecal microbiota suspension from the BE group orally, respectively, for 14 days. Compared with the Ctrl group, dietary supplementation with BE improved growth performance by increasing the average daily gain and average daily food intake and reducing the fecal score in weaned piglets. Dietary supplementation with BE also improved intestinal morphology and cell apoptosis by increasing the ratio of villus height to crypt depth and decreasing the average optical density of apoptotic cells; meanwhile, improvements also involved attenuating oxidative stress and intestinal barrier dysfunction by increasing the total antioxidant capacity, glutathione, and catalase, and upregulating the mRNA expressions of Occludin, Claudin-1, and ZO-1. Interestingly, the oral administration of a fecal microbiota suspension to piglets fed BE had similar effects to those of the BE group. According to 16S rDNA sequencing analysis, dietary supplementation with BE altered the composition of the microbiota, including firmicutes, bacteroidetes, lactobacillus, phascolarctobacterium, and parabacteroides, and increased the metabolites of propionate and butyrate. In addition, Spearman analysis revealed that improvements in growth performance and intestinal damage were significantly correlated with differential bacteria and short-chain fatty acids (SCFAs). In brief, dietary supplementation with BE improved the growth performance and intestinal damage by altering the gut microbiota composition and SCFAs in weaned piglets.

Association between adherence to a low carbohydrate dietary (LCD) pattern with breast milk characteristics and oxidative markers in infants’ urine: a cross-sectional study

BackgroundBreast milk (BM) is a dynamic fluid that varies over time and between women. The variations in BM components are most likely associated with maternal diet quality. This study aimed to assess adherence to a low carbohydrate dietary (LCD) pattern with oxidative stress markers of BM characteristics and infants’ urine.Materials and methodsIn this cross-sectional study 350 breastfeeding mothers and their infants were recruited. BM samples were collected from mothers, and urine specimens were obtained from each infant. To evaluate LCD scores, subjects were divided into 10 deciles according to the percent of energy obtained from carbohydrates, proteins, and fats. Determination of total antioxidant activity was conducted using the ferric reducing antioxidant power (FRAP), 2, 2′-diphenyl-1-picrylhydrazyl (DPPH), thiobarbituric acid reactive substances (TBARs), and Ellman’s assay. Biochemical assays of samples including calcium, total protein, and triglyceride level were also performed using commercial kits.ResultsParticipants with the greatest LCD pattern adherence were placed into the last quartile (Q4), and those with the minimum LCD were in the first quartile (Q1). Individuals in the highest LCD quartile had significantly higher levels of milk FRAP, thiol, and protein, as well as infant urinary FRAP and lower milk MDA levels than those in the lowest quartile. Multivariate linear regression analyses indicated that higher score of the LCD pattern was associated with a higher level of milk thiol, protein, and lower level of milk MDA (p < 0.05).ConclusionOur findings show that adherence to a LCD, as defined by a low level of carbohydrates in daily food intake, is linked with improved BM quality and markers of oxidative stress in infant urine.

Dietary intake of macro and micronutrients correspond with sleep behaviors in collegiate female soccer players

Both macro and micronutrient intakes have an impact on sleep behaviors in athletes, notably sleep onset latency and sleep efficiency. Proper nutrient intake supports daily muscle repair and synthesis of sleep regulating compounds (e.g., serotonin and melatonin). The objective of this study was to evaluate the relationship between sleep behaviors and dietary intake in elite college female athletes. It was hypothesized that dietary carbohydrates and vitamin intake would be associated with both sleep timing and consistency. Twenty-three female Division I collegiate soccer players were evaluated for their sleep and nutritional patterns. Athletes wore an OURA ring (OURA Health, Oulu, Finland) each night for 31 days during the competitive season. During the final three days, athletes recorded their dietary intake using the Automated Self-Administered 24-hour Dietary Assessment Tool (ASA24). All athletes were exposed to the same travel schedule throughout the evaluation period, which did not differ by more than one time zone. Pearson’s R with Bonferonni Correction was used to assess the relationship between the average daily intake of various nutrients with average nightly sleep behaviors. Total Vitamin B12 ( r=-0.63), added Vitamin B12 ( r=-0.62), and Vitamin C ( r=-0.53) were negatively correlated with average bedtimes (P&lt;0.01) while added Vitamin B12 ( r=-0.61) and Vitamin C ( r=-0.50) were also negatively associated with wake times (P&lt;0.01). Greater dietary intake of kcals from carbohydrates, with respect to recommended consumption, was associated with earlier bedtimes ( r=0.41, P&lt;0.05). In conclusion, greater intake of carbohydrates and vitamins B12 and C are associated with ideal sleep behaviors. A potential mechanism accounting for this relationship could be the enhanced contribution of antioxidants and glycemic load on the synthesis of vital hormones for sleep regulation, including serotonin and melatonin. This is the full abstract presented at the American Physiology Summit 2023 meeting and is only available in HTML format. There are no additional versions or additional content available for this abstract. Physiology was not involved in the peer review process.

Ethanol and Methyl Jasmonate Fumigation Impact on Quality, Antioxidant Capacity, and Phytochemical Content of Broccoli Florets during Storage

Broccoli is a vegetable that offers valuable components, such as glucosinolates (GLS), flavonoids, and hydroxycinnamic acids (HCA), for our daily food intake. These substances have been associated with reducing the risk of cancer and cardiovascular diseases (CVD). Broccoli florets are also highly perishable, given their elevated respiration rates and their sensitivity to ethylene. Experiments have been carried out on broccoli to investigate the consequences of abiotic stress post-harvest treatments in retarding the senescence process. Nevertheless, the influence of these treatments on the phytochemicals of broccoli has not been extensively examined. Florets of broccoli (Brassica oleracea) were exposed to an atmosphere consisting of 10,000 µL.L−1 ethanol at room temperature for 30 min and 120 min. The exposure to methyl jasmonate (MeJA) treatments was carried out at room temperature using 1 µL.L−1 for 45 min and 180 min. The yellowing of florets was delayed using 10,000 µL.L−1 of ethanol at both exposure times as compared to untreated florets, and the chlorophyll titers were also superior with both doses over the control. The total phenols of the florets increased by 15% and 18% with the application of the hormetic and high doses, respectively, throughout the storage period compared to unexposed broccoli. The GLS and HCA yields were also increased by both ethanol doses. The exposure of florets to 1 µL.L1 MeJA for 45 min resulted in delayed yellowing of florets; however, longer exposures resulted in yellowing after 21 d and significantly (p &lt; 0.05) increased respiration rates relative to untreated florets. Overall antioxidant capacity of the florets was significantly reduced with both doses of methyl jasmonate; however, HCA titers were increased at both doses. The amount of total glucobrassicins within broccoli was increased following exposure of florets to both doses, but no significant differences in glucoraphanin content were observed. As a conclusion, the ethanol treatment could indeed delay senescence and lead to the induction of phytochemicals. In contrast, MeJA’s effect on quality is not quite substantial; it can, however, be used to improve the phytochemical content of florets, particularly indole-type GLS.