Cytomegalic Inclusion Bodies Research Articles

Diagnosis of inflammatory diseases of the CNS in children by means of CSF-protein electrophoresis (author's transl)

CSF-protein was analyzed by quantitative zone electrophoresis in agarose gel in 141 children and adolescents who suffered from acute and chronic inflammatory disease of the CNS. 67 patients without any CNS affection served as controls. The normal CSF-protein consists of 15 fractions: 2 prealbumin, albumin, 5 α- 3 β- and 4 γ-globulins. In case of aseptic and purulent meningitis the protein profile of blood-CSF-barrier disturbance was found in a varying degree. The relation of nearly all protein fractions had changed, prealbumin, γ- and γ4-globulin, typical of CSF, were decreased in different amounts. The results in 42 children with aseptic meningitis differed significantly not only from that in the control group but also in the 21 patients with purulent meningitis. 179 patients (further 49 children added) belonging to one of these diagnostic groups were correctly classified in about 90 % using discriminant analysis. 50 children and adolescents with chronic inflammatory diseases of the CNS showed the protein pattern of local immunoglobulin synthesis. 4/5 of 29 children with congenital infections (cytomegalic inclusion body disease, toxoplasmosis, rubella and syphilis) revealed a marked increase of the γ 3-globulin, in 1/3 of these patients oligoclonal γ-fraction appeared. All patients with multiple sclerosis (6 adolescents) and subacute sclerosing panencephalitis (15 children) revealed an oligoclonal γ-globulin pattern. In MS the oligoclonal γ-bands were distributed in a stepwise fashion, whereas in SSPE there were 2-4 markedly protruding fractions.

ADULT T‐CELL LEUKEMIA WITH GENERALIZED CYTOMEGALIC INCLUSION DISEASE AND PNEUMOCYSTIS CARINII PNEUMONIA

An autopsy case of adult T-cell leukemia with generalized cytomegalic inclusion disease and pneumocystis carinii pneumonia is reported. Tumor cells had T-cell characteristics (E-rosette) and cerebriform nucleus similar to Sèzary cells. Generalized lymphadenopathy, hepatosplenomegaly and an ectopic pancreas in the ileum were found at the time of autopsy. Histologically, leukemic infiltration was observed in almost every organ, and perivascular infiltration, vascular invasion were conspicuous findings. Cytomegalic inclusion bodies were observed in most organs (lungs, salivary glands, pancreas, liver, ectopic pancreas, sweat gland, stomach, thyroid gland, pituitary body, etc.). An acute hepatitis, probably caused by cytomegalovirus, was also noted. Presumed correlation of adult T-cell leukemia, cutaneous T-cell lymphoma and T-cell lymphoma was discussed.

Acute multiple gastric ulcers in the pyloric antrum.

Acute prepyloric hemorrhagic erosions occasionally progress to symmetrical ulcers. Cytomegalic inclusion bodies were frequently seen, a history of alimentary allergens, excessive alcohol intake or coffee drinking was common. Hyperchlorhydria which decreased spontaneously was found in 89% during the acute attack.

Oligoclonal gamma-globulin banding of cerebrospinal fluid in patients with subacute sclerosing panencephalitis: Comparison of the electrophoretic pattern with that in multiple sclerosis and congenital infections

Lumbar cerebrospinal fluid (CSF) of 8 patients with subacute sclerosing panencephalitis (SSPE) was examined by agarose gel electrophoresis. In comparison with normal controls and children with different neurological diseases (including infections, tumours and degenerative diseases) the quantitative evaluation of the pherograms by an analog computer revealed an extreme change of the γ-globulin profile. All cases showed 6–7 abnormal subfractions consisting of 2–4 tall, markedly protruding spikes and several smaller intermediate fractions. The oligoclonal γ-globulin contributed 20.1–42.5% to total protein. This particular γ-globulin profile seems to be highly indicative of the diagnosis of SSPE. It can be distinguished from the oligoclonal pattern in patients suffering from multiple sclerosis (MS) and congenital infections. The CSF protein profile of 13 patients with MS was different from that in SSPE in that it showed 1–5 monoclonal γ-fractions in every case with none or only one peak protruding more markedly. The percentage of all subfractions amounted to 4.5–23.8% of total protein. As in MS, the aspect of oligoclonality in 9 children with congenital infections (cytomegalic inclusion body disease, toxoplasmosis and rubella) was quite variable, as again 1–5 abnormal subfractions were detected. Their relative concentrations, on the whole ranging from 0.6–12% of total protein, was considerably lower than in SSPE.

Pathology of Cytomegalic Inclusion Body Disease in Swine

Ilistopathological and electron microscopical studies were performed to in-vestigate lesions in a series of eight cases (7 to 180 days of age) of porcine cytomegalicinclusion body disease (inclusion body rhinitis) encountered in several hog yards inTowada City, Aomori Prefecture. The characteristic feature noted was the formation ofintranuclear inclusion bodies in the tubuloalveolar glandular epiLhelium of the nasalmucosa. These bodies were classified into three distinct types. In four cases, intranuclearinclusion bodies were demonstrated also in various other organs throughout the body, such as the trachea, bronchioles, salivary glands, lacrimal glands, kidneys, adrenals andNymph nodes. Associated frequently with the inflammatory changes of the nasal mucosawere splenic extramedullary hematopoiesis, glomerular nephritis and nonpurulentencephalitis. Electron micrographs of the nasal mucosa demonstrated intranuclear cap-sids and enveloped particles in cytoplasmic vacuoles and in the glandular lumen.

Disseminated cytomegalic inclusion disease in an adult with cirrhosis of liver and review of literatures

We reported a 44 year old man with micronodular cirrhosis who eventually died from massive hematemesis and melena. At postmortem studies, there were disseminated cytomegalic inclusion bodies detected in various organs. In liver, intranuclear inclusion bodies were observed in cirrhotic liver. We discussed here possible pathogenesis of CMV (cytomegalovirus) infection in adults, in reviewing literatures.

Extensive central nervous system calcification in a stillborn male infant due to cytomegalovirus infection

The radiologic and pathologic findings of a stillborn male fetus with cytomegalic inclusion disease are described. Massive calcification of the cerebrum, cerebellum, eye (sclera and choroid), and spinal cord was present. There were unique deformities and apparent aplasia of some of the ribs. Classic intranuclear cytomegalic inclusion bodies were present primarily in the kidney. Routine radiography of stillborn infants is recommended for further study of intrauterine growth abnormalities.

Gastrointestinal hemorrhage secondary to cytomegalovirus after renal transplantation: A case report and review of the problem

Generalized cytomegalovirus infection was associated with massive and ultimately fatal upper gastrointestinal bleeding in a renal allograft recipient and persisted even after subtotal gastric resection. The surgical specimen and the remaining stomach at autopsy revealed multiple superficial ulcérations with cytomegalic inclusion bodies within the gastric mucosa. Renal failure in the terminal stages of the patient's illness required hemodialysis but did not seem to be the sole result of allograft rejection, suggesting that the renal dysfunction may be caused by the systemic viral infection.

Unusual roentgenological findings in cytomegalic inclusion body disease: Large and circumscribed calcareous deposits of the basal ganglia and scattered calcifications of the parieto-occipital cortex

The roentgenological and clinical findings of a five year old girl suffering from cytomegalic inclusion body disease are reported. In this disease unusual circumscript and large calcareous deposits of the basal ganglia and scattered gyriform calcifications of the occipito-parietal cortex were shown on plain skull x-rays and their paraventricular localization demonstrated on pneumoencephalotomogram.

THICK GASTRIC FOLDS IN CHILDHOOD

THICK GASTRIC FOLDS IN CHILDHOODRALPH S. LACHMAN, M.D., DAVID J. MARTIN, M.D., M.R.C.P. and GORDON F. VAWTER, M.D.Audio Available | Share

Types of probable virus encephalitis in Norwegian autopsy material

As reports of the occurrence of viral encephalitides from the Scandinavian countries have been few, the authors have reviewed an autopsy series from Rikshospitalet, Oslo, Norway. The investigation covered the years from 1952 to 1966, and 35 cases of probable virus encephalitis were collected. The cases could be separated into 4 groups according to clinical symptoms and histopathological picture. The first group corresponded well to the picture of an acute herpes simplex encephalitis with inclusion bodies, although the virus was isolated in brain tissue from the autopsy once only. In the second group the course of the illness varied greatly from 1 month up to nearly 8 years in 1 case. The histological findings were those of a diffuse subacute to chronic viral type of infection, and inclusion bodies were found in 6 of the cases including the one of 8 years' duration. The third group comprised acute and more prolonged cases, but the histopathological findings were sparse and uncharacteristic. The fourth group consisted of children with cytomegalic inclusion body disease in whom the brain was involved. The EEG findings are discussed. When repeated recordings were available variations occurred, apparently mirroring various phases in the course of the illness. The symptomatology, diagnostic problems and aetiology are discussed.

Mumps Virus Infection of the Pregnant Hamster

ABSTRACT The objective of our present studies has been to follow the course of mumps virus when injected intravenously into pregnant hamsters during early stages of gestation, in order to determine possible relations to fetal disease and/or malformations. Several considerations prompted the selection of mumps virus for these investigations. One was that, while rubella (Gregg, 1941) and cyto-megalic inclusion body disease (Weller & Hanshaw, 1962) have been the only two viruses shown to have a definite cause-effect relation in the etiology of human congenital malformations, there has been a continuing suspicion that mumps virus may also act as a teratogenic agent in human pregnancy (Kaye & Reaney, 1962; Blattner & Heys, 1961; Hyatt, 1961). A second reason was that mumps virus has a natural pathogenicity for hamsters (Kilham & Overman, 1953). In addition, this agent is capable of infecting women at term, the strain used in present experiments having been obtained from human milk a few days post-partum (Kilham, 1951).

Cytomegalic inclusion—body disease in sheep a report of two cases

Cytomegalic inclusion—body disease in sheep a report of two cases

CYTOMEGALIC INCLUSION DISEASE ASSOCIATED WITH HYPOPARATHYROIDISM

The presence of both cytomegalic inclusion body disease and hypoparathyroidism seems well established in the case reported here. Symptoms of the former antedate the onset of symptoms characteristic of the latter. A more definite causal relationship could be established only by surgical exploration of the neck and the finding of cytomegalic inclusions in the parathyroid tissue. The latter procedure could not be justified under the circumstances, so the possible etiologic relationship must rest upon circumstantial evidence.

Spontaneous cytomegalic inclusion body disease involving lacrimal glands of caesarian-derived (so-called) pathogen-free rats.

HISTOLOGICAL study of lacrimal glands in laboratory rodents revealed cytological changes, for example, nuclear polymorphism and inclusions characteristic of cytomegalic inclusion body disease in rats of the Sprague–Dawley strain; no other organs were involved. Lesions occurred in both intra- and ex-orbital glands of males only at an age of six months or older and with an incidence of 100 per cent. Similar observations have recently been published for eight rat colonies of different geographical locations1. The disease is apparently widespread among laboratory rats. Guinea pigs, a variety of mouse strains, both golden and Chinese hamsters, Wistar and Fisher rats, either commercially obtained or bred and raised in the Roscoe B. Jackson Laboratory, were free of the disease.

Placental transmission of viruses: With especial reference to the intrauterine origin of cytomegalic inclusion body disease

Placental transmission of viruses: With especial reference to the intrauterine origin of cytomegalic inclusion body disease

Cytomegalic inclusion-body disease occurring in twins.

Cytomegalic inclusion-body disease occurring in twins.