Cytb Gene Sequences Research Articles

Genetic variations suggests that Takifugu rubripes, T. chinensis, and T. pseudommus are the same species with a shared gene pool

IntroductionPufferfish are valuable ingredients and important fishery resources in Asia. Among them, approximately 25 Takifugu species have undergone explosive speciation in the marine environments of East Asia. Specifically, T. rubripes, T. pseudommus, and T. chinensis are remarkably similar in appearance and genetics, and their classification remains controversial. Here, we provide comprehensive genetic and genomic evidence regarding their speciation and classification.MethodsWhole genome sequencing was performed on T. pseudommus, leading to the identification and development of 15 novel tandem simple sequence repeats (SSRs) from its genomic data. Genetic diversity, differentiation, and population structure among T. rubripes, T. pseudommus, T. chinensis, and T. xanthopterus were subsequently analyzed using a combination of these 15 SSR loci and mitochondrial cytochrome oxidase subunit I (CoI) and cytochrome b (CytB) gene sequences. Furthermore, genomic variations, including single nucleotide polymorphisms, insertions, and deletions, were identified by comparing the genome of T. pseudommus with the reference T. rubripes genome retrieved from the National Center for Biotechnology Information. These variations were screened using ENSEMBL annotation and gene ontology analysis, and their potential association with morphological differences, such as dorsal spot patterns, was evaluated using reference Takifugu specimens.Results and discussionPopulation genetic analysis of T. rubripes, T. pseudommus, and T. chinensis using the CoI and CytB genes, combined with the 15 SSR loci, revealed clustering into a single genetic group characterized by remarkably low genetic diversity (four haplotypes, with diversity values ranging from 0.0000 to 0.00065) and minimal pairwise differentiation (microsatellite-based FST values ranging from -0.0021 to 0.0075). Additionally, comparative genomic analysis between T. pseudommus and the reference T. rubripes genome did not identify genetic variations that could directly explain the observed morphological differences among the species. These findings strongly suggest that T. rubripes, T. pseudommus, and T. chinensis represent a single species sharing a common genetic pool.

Genetic Diversity of Cultured Rainbow Trout (Oncorhynchus mykiss) Populations in Turkey Based on Mitochondrial DNA Cytochrome b (cyt-b) Sequence Analysis

This study aimed to investigate the genetic diversity and structure of rainbow trout (Oncorhynchus mykiss) cultured populations in different provinces of Turkey, based on the cytochrome b (cyt-b) gene region of mitochondrial DNA (mtDNA). Tissue samples were collected from a total of 98 fish (seven fish per province) across 14 provinces, followed by DNA isolation. The mtDNA cyt-b gene region (754 bp) was amplified using PCR. Genetic diversity indices, genetic structure, and phylogenetic analyses were calculated from the cyt-b gene sequence data. The average nucleotide frequencies of the four nucleotides cytosine (C), thymine (T), adenine (A), and guanine (G) were found to be 26.2%, 24.9%, 26.2%, and 22.8%, respectively, with a higher A + T content (51%) compared to G + C content (49%). A total of 645 polymorphic loci were identified across the 14 populations, and 50 distinct haplotypes were defined. Haplotypic diversity ranged from 0.91 to 0.48, while nucleotide diversity (π) varied between 0.26 and 0.00. The highest genetic diversity was observed in the Tokat and Van populations, whereas the lowest was recorded in the Elazığ and Kahramanmaraş populations. AMOVA analyses revealed that 71.1% of the genetic diversity was found between populations, while 28.9% was found within populations. Pairwise FST values ranged from 0.38 to 0.99, with an average FST of 0.71. Phylogenetic analyses indicated that the populations clustered into two main groups, with further sub-groupings within these clusters. Notably, the Kahramanmaraş and Elazığ populations were found to be significantly differentiated from other populations. In conclusion, despite rainbow trout not being a native species in Turkey, the findings of this study indicate that the genetic diversity of the populations is at a good level. However, in order to preserve the genetic diversity of cultured rainbow trout populations and to increase effective population size, it is recommended that breeders engage in fry exchanges and import new rainbow trout specimens to enhance genetic diversity.

Sensitivity Analysis of Pyrenophora tritici-repentis to Quinone-Outside Inhibitor and 14α-Demethylase Inhibitor Fungicides in Latvia

Tan spot caused by Pyrenophora tritici-repentis is a severe threat to wheat production in all major wheat-growing regions. Sustainable tan spot control can be achieved by an integrated approach, including responsible management of fungicide sprays. The data about the sensitivity of P. tritici-repentis to various fungicides in the Baltic Sea region are rare. In this study, we described the variation of P. tritici-repentis sensitivity to four fungicide active ingredients to detect the formation of resistance to the most commonly used quinone-outside inhibitor (QoI) and 14α-demethylase inhibitor (DMI) fungicides in the pathogen’s population in Latvia. The effect of prothioconazole, mefentrifluconazole, pyraclostrobin, and azoxystrobin on 93 P. tritici-repentis strains from various hosts was tested in vitro by assessing mycelium linear growth inhibition at three different active ingredient concentrations (0 0.01, 0.1 and 0.5 mg L−1). Pathogen sensitivity significantly (p < 0.001) varied between the fungicide active ingredients and strains. The prothioconazole (concentration 0.5 mg L−1) had the most significant effect, with a median mycelial growth inhibition of 70.34%, followed by pyraclostrobin (47.02%), azoxystrobin (24.24%), and mefentrifluconazole (11.11%). Mutation G143A was detected in cytb gene sequences and confirmed the resistance formation in Latvia’s P. tritici-repentis population, while F129L and G137R mutations were absent. This study provided insight into P. tritici-repentis population’s sensitivity to active ingredients of DMI and OoI fungicide groups, helping to fill the knowledge gap about the pathogen fungicide sensitivity in this region.

Mitochondrial Genomes of Streptopelia decaocto: Insights into Columbidae Phylogeny.

In this research, the mitochondrial genome of the Streptopelia decaocto was sequenced and examined for the first time to enhance the comprehension of the phylogenetic relationships within the Columbidae. The complete mitochondrial genome of Streptopelia decaocto (17,160 bp) was structurally similar to the recognized members of the Columbidae family, but with minor differences in gene size and arrangement. The structural AT content was 54.12%. Additionally, 150 mitochondrial datasets, representing valid species, were amassed in this investigation. Maximum likelihood (ML) and Bayesian inference (BI) phylogenetic trees and evolutionary time relationships of species were reconstructed based on cytb gene sequences. The findings from the phylogenetic evaluations suggest that the S. decaocto was classified under the Columbinae subfamily, diverging from the Miocene approximately 8.1 million years ago, indicating intricate evolutionary connections with its close relatives, implying a history of species divergence and geographic isolation. The diversification of the Columbidae commenced during the Late Oligocene and extended into the Miocene. This exploration offers crucial molecular data for the S. decaocto, facilitating the systematic taxonomic examination of the Columbidae and Columbiformes, and establishing a scientific foundation for species preservation and genetic resource management.

Taxonomic resolution of the hillstream suck-loach Beaufortia pingi species group (Cypriniformes, Gastromyzontidae) and two new species from Southwest China– Beaufortia granulopinna and Beaufortia viridis

Two new species, Beaufortia granulopinna and Beaufortia viridis, are described from the upper Pearl River system in southwest China. Both species share the characteristics of the Beaufortia pingi species group, including prominent vertical stripes on the lateral body and pinnate-type lower lips, distinguishing them from other Beaufortia species. Beaufortia granulopinna is differentiated from other species in the group by possessing a unique set of characteristics: the presence of well-developed prominent tubercles on the first 6–9 pectoral fin rays in adults; and a significant proportion (54.76%) of individuals experiencing blurriness or absence of vertical stripes in the mid-section of the lateral body upon reaching adulthood. The minimum interspecific genetic distance within the genus based on mitochondrial cytb gene sequences is 10.80%. Beaufortia viridis is distinguished from other species in the group by consistently exhibiting vertical stripes of uniform length, width, and spacing across all stages of growth; the absence of tubercles on the branched rays of pectoral fins; and a body coloration of dark cyan to green. The minimum interspecific genetic distance within the genus based on mitochondrial cytb gene sequences is 4.60%. Molecular phylogenetic results confirm that the Beaufortia pingi species group forms a monophyletic clade, which is congruent with morphological classification findings. This study also addresses and resolves the taxonomic ambiguity surrounding Beaufortia pingi and Beaufortia zebroida, providing a redescription of these taxa.

Comparative Study of Two Himalayan Snow Trouts, Schizothorax esocinus and Schizothorax curvifrons Within the Schizothoracinae and Other Nearest Relatives of Cyprinidae, Inferred from Mitochondrial Sequences of Cytochrome b (Cyt-b) and Cytochrome Oxidase I (Co-I) Gene.

The Himalayan region encompasses varied aquatic ecosystems, characterized by the presence of diverse ichthyofauna, particularly represented by members of the Schizothorax genus, commonly referred to as snow trout. The primary objective of this work was to examine the molecular phylogeny of Schizothoracinae, specifically focusing on the two species, Schizothorax esocinus and Schizothorax curvifrons, which are known to inhabit the northern and north-eastern regions of the Himalayas. This investigation was conducted by analyzing the entire mitochondrial Cyt-b and Co-I gene sequences. The aligned Cyt-b and Co-I sequences for S. esocinus, S. curvifrons, and related members within the subfamily Schizothoracinae, spanned 1130 to 1141 and 1536 to 1551 base pairs, respectively. Using these gene, phylogenetic trees were created to compare Schizothoracinae species to other subfamilies of the family Cyprinidae (Barbinae, Alburninae, Leuciscinae, Xenocyprinae, Cyprininae, and Cultrinae). Genetic distances for Cyt-b and Co-I sequence at three hierarchical levels shows significant disparities in their average score. For Cyt-b, average p-distances for intraspecies, intragenus, and intrafamily were 2.13%, 4.1%, and 15.23%, respectively. Similarly, for Co-I, average p-distances were 1.19%, 3.6%, and 13.8% for intraspecies, intragenus, and intrafamily, respectively. Total number of haplotypes (h) based on Cyt-b and Co-I gene were 6 and 12 within the target Schizothorax spp. In the present study, the observed range of haplotype diversity (hd) for the Cyt-b gene varied from 0.00 to 0.847, with an average haplotype diversity of 0.847 ± 0.034. Similarly, for the Co-I gene, the observed haplotype diversity ranged from 0.00 to 0.931, with an average value of haplotype diversity estimated to be 0.931 ± 0.024. The results of the present study clearly shows that the representative species exhibited close affinities with members of Barbinae and Cyprininae, while other subfamilies formed distinct groups. The findings of the study also indicated that the Cyt-b and Co-I gene exhibits polymorphism and has the potential to serve as a marker for identifying genetic differentiation among populations based on ecological habitats. Mitochondrial Cyt-b and Co-I have been established as a universally accepted and validated genetic marker within a comprehensive bio-identification system at the species level.

Genetic diversity of the common carp black strain population based on mtDNA (D-loop and cytb)

The common strain black carp (Cyprinus carpio var. baisenensis) is a culturally important carp strain that is raised and cultured in Guangxi Province, China. Its color reflects the interactions between the Burau people and their surrounding environment. The population of the common carp black strain was isolated and cultured in a rice-fish integration system. To explore the genetic diversity and protection of germplasm resources, we analyzed mitochondrial DNA (mtDNA) sequences, specifically the displacement loop (D-loop) and cytochrome b (Cytb), using single-nucleotide polymorphisms (SNP). We compared these sequences with those from four other local common carp populations. The study included a total of 136 adult common carps from five strain populations: the common black carp strain (HJ = 31), Jian (F = 30), Heilongjiang (H = 10), Songpu (S = 31), and Saijiang (SJ = 34). The results of the Cytb and D-loop analyses showed that the Heilongjiang carp (H) and Saijiang (SJ) populations had the highest levels of haplotype diversity (0.867 ± 0.034785) and nucleotide diversity (π = 0.0063 ± 0.000137 and 0.0093 ± 0.000411), respectively. On the other hand, the Common carp black strain population (HJ) exhibited the lowest haplotype diversity in both Cytb and D-loop, with haplotype 2 being the most commonly observed among the populations. Private haplotypes dominated the five common carp populations, which were significantly different at P<0.001. Furthermore, analyzing the coefficient of genetic differentiation (Fst), the highest genetic difference was observed between Saijiang (SJ) and Heilongjiang (H) (Fst = 0.963), whereas the lowest was observed between Songpu (S) and the Common carp black strain population (HJ) (Fst = 0.019) for the Cytb gene sequences. For the D-loop, the Common carp black strain population (HJ) and Songpu (S) (Fst = 0.7) had the highest values, and Heilongjiang (H) and Common black carp strain (HJ) had an Fst of 0.125. Additionally, the AMOVA analysis revealed a higher level of variance for the Cytb and D-loop genes, indicating lower genetic diversity within the local carp community. On the other hand, the phylogenetic tree analysis showed that the five carp populations were closely related and formed a distinct cluster. The distinct cluster of populations suggests a common ancestor or recent gene flow, possibly due to geographic proximity or migration history, and unique genetic characteristics, possibly due to adaptations or selective pressures. The results of this study provide valuable insights into the genetic diversity of the common strain black carp, which can have implications for conservation, breeding programs, evolutionary studies, and fisheries management.

Identification of Cervi Cornu (Cervus elaphus) and its formula granules based on PCR-RFLP

Cervi Cornu is the ossified antler, or the base antler that falls off in the spring of the following year after the pilose antler is sawn off from Cervus elaphus or C. nippon, as a precious traditional Chinese medicine, has been recognized for its medicinal value and widely used in clinical practice. However, the origins of Cervi Cornu are miscellaneous, and Cervi Cornu is even mixed with adulterants in the market. Currently, there is a shortage of ways to identify Cervi Cornu and no standard to control the quality of Cervi Cornu. So it is valuable to develop a way to effectively identify Cervi Cornu from the adulterants. In this study, the differences in the mitochondrial barcode cytochrome b(Cytb) gene sequences of C. elaphus, C. nippon and their related species were compared and the specific single nucleotide polymorphism(SNP) sites on the Cytb sequences of Cervi Cornu were screened out. According to the screened SNPs, Cervi Cornu-specific primers dishmy-F and dishmy-R were designed. The PCR system was established and optimized, and the tolerance and feasibility of Taq polymerases and PCR systems affecting the repeatability of the PCR method were investigated. The amplification products of C. elaphus and C. nippon were digested using the restriction enzyme MseⅠ. The results showed that after electrophoresis of the product from PCR with the annealing temperature of 56 ℃ and 35 cycles, a single specific band at about 100 bp was observed for C. elaphus samples, and the product of C. elaphus samples was 60 bp shorter than that of C. nippon samples. There was no band for adulterants from other similar species such as Alces alces, Rangifer tarandus, Odocoileus virginianus, O. hemionus, Cap-reolus pygargus, Przewalskium albirostis and negative controls. The polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) method established in this study can quickly and accurately identify Cervi Cornu originated from C. elaphus in crude drugs, standard decoctions, and formula granules, and distinguish the origins of Cervi Cornu products, i.e., C. nippon and similar species. This study can be a reference for other studies on the quality standard of other formula granules of traditional Chinese medicines.

EDNA Metabarcoding: Gaps of Publicly Available Reference Databases of Freshwater Vertebrates in Sri Lanka

Environmental DNA (eDNA) has become a rapidly emerging technology during the last decade. Although eDNA applications are used worldwide, none of the eDNA metabarcoding studies have been conducted solely in Sri Lanka yet. Detection rate of species through DNA metabarcoding technique varies with the availability of gene sequences in reference databases. Therefore, the main objective of this study was to assess the data gaps of local species in two selected reference databases (the NCBI database and the BOLD database). Local freshwater vertebrates were considered for this analysis by dividing them into three major categories as freshwater fish (97 species), freshwater amphibians (40 species) and freshwater reptiles (7 species). The availability of COI gene sequence and CytB gene sequence of each species were recorded. In the NCBI database, reference gene sequences were available only for 17.53% (17 species) of freshwater fish, 7.50% (3 species) of freshwater amphibians and 14.29% (1 species) of freshwater reptiles. In the BOLD database, reference gene sequences were available for 52.58% (51 species) of freshwater fish, 17.50% (7 species) of freshwater amphibians and 42.86% (3 species) of freshwater reptiles. Endemic species were analyzed separately. This revealed the availability of reference gene sequences for 4.92% (3 species) of endemic freshwater fish in the NCBI database. However, none of the reference gene sequence was found for endemic freshwater amphibians and reptiles. In the BOLD database, reference gene sequences were available for 36.07% (22 species) of endemic freshwater fish and 33.33% (1 species) of endemic freshwater reptiles but none for endemic freshwater amphibians. According to the results, large gaps of publicly available reference CO1 gene sequences were observed in both NCBI and BOLD databases. Not only the availability of reference gene sequences, but also the submitted countries of those sequences were also analyzed as a part of this study. Asia has contributed the most according to the country-vice analysis. Among them, many are in the South Asian region. Therefore, this study highlights the need of expanding reference databases for greater local representation as well as the importance of forging collaborations with the developed countries in the same region in order to increase international cooperation and funding to carry on further studies on neglected taxa and under‐represented taxa in Sri Lanka. &#x0D; Keywords: eDNA (environmental DNA), Metabarcoding, Reference databases, Reference gene sequences

First record of Dermanura gnoma for the threatened Pernambuco Endemism Center, northern Atlantic Forest, with phylogenetic insights.

Dermanura gnoma (Handley, 1987) is a phyllostomid bat that occurs in South America. In Brazil, most of the records are concentrated in the Amazon Forest, with sparse data from Cerrado and Atlantic Forest. This study presents the first record of D. gnoma for Alagoas state, and for the Pernambuco Endemism Center (PEC), the most threatened region of the Brazilian Atlantic Forest, characterized by its high rate of endemism, habitat loss, and fragmentation. The species was surveyed at Murici Ecological Station and its identification was supported by morphological and molecular data. We provide the first COI and CYTB gene sequences for this species in the entire Atlantic Forest. Phylogenetic analyses reveal that the generated sequences were recovered in the same clade as the published sequences of D. gnoma from the Amazonian Forest, corroborating the disjunct distribution pattern between Amazonia and PEC, as already reported for other vertebrate taxa. Also, this record increases the number of bat species to 71 for the PEC and extends the geographic distribution of D. gnoma by 758 kilometers to the northeast of its range.

Formosaniaimmaculata, a new species of hillstream loach (Teleostei, Cypriniformes, Gastromyzontidae) from the Ou-Jiang River, Southeast China.

Formosaniaimmaculata, a new species, is described from the Ou-Jiang basin in Zhejiang Province, Southeast China. It is distinguished from other species of the genus by having a combination of the following characteristics: body without obvious mottling; snout length longer than postorbital length; abdominal scaleless area extending to middle of pectoral-fin base; shorter rostral barbels, the outermost pair length 112.9%-140.0% of eye diameter; and shorter lower lip papillae, length 19.9%-24.4% of eye diameter. Its validity is also affirmed by its distinct Cytb gene sequence divergence from all congeners and its monophyly recovered in a Cytb gene-based phylogenetic analysis.

Molecular insights on the historical dispersion of Piezodorus guildinii (Hemiptera: Pentatomidae) in Brazil.

Piezodorus guildinii (Westwood, 1837) (Hemiptera: Pentatomidae) is an important arthropod pest of soybean (Glycine max (L.) Merr.) throughout American continents. However, the historical events associated with its dispersion are poorly understood. In this study, we employed a phylogeographic approach to investigate the origin and demographic history of P. guildinii in Brazil. We analyzed the cytochrome c oxidase subunit I and Cytb gene sequences of P. guildinii individuals collected in Brazil's 5 soybean production macro-regions and cross-referenced this information with sequences available in public databases. Our findings support an older Caribbean basin establishment for the current genealogical strains of P. guildinii, with subsequent dispersion to Brazil around 0.97 Mya. No secondary dispersion of this species from the Caribbean region to soybean areas in Brazil was identified. The Brazilian populations of P. guildinii are genetically structured across the country's soybean macro-regions and show strong signals of continuous demographic and spatial expansion in Brazil, which may be accelerated by the soybean cropping landscape in the country. The populations from the northern region (MR5) are older than the Central and South populations. The signs of demographic expansion indicate that P. guildinii populations are increasing their effective size in soybean regions, which could reflect its importance as a soybean pest in the coming years.

Molecular detection of Bartonella quintana, Acinetobacter baumannii and Acinetobacter haemolyticus in Pediculus humanus lice in Nigeria, West Africa.

The human lice Pediculus humanus is distributed worldwide but, it thrives and flourishes under conflict situations where people are forced to live in crowded unhygienic conditions. Molecular methods were used to identify and screen human lice for the DNA of pathogens of public health importance in an area that has been under insurgency related to religious and political conflicts with tens of thousands of internally displaced people (IDP). DNA of Bartonella quintana, Acinetobacter baumannii and Acinetobacter haemolyticus was detected in 18.3%, 40.0% and 1.7%, respectively, of human lice collected from children in Maiduguri, Nigeria. More body lice than head lice were positive for pathogen's DNA (64.3% vs. 44.4%; χ2 = 1.3, p = 0.33), but the difference was not significant. Two lice samples were found to harbour mixed DNA of B. quintana and A. baumannii. Phylogenetic analysis of the cytochrome b (cytb) gene sequences of the positive lice specimens placed them into clades A and E. This is the first report on the molecular identification of human lice and the detection of the DNA of pathogens of public health importance in lice in Nigeria, West Africa. The findings of this study will assist policy makers and medical practitioners in formulating a holistic healthcare delivery to IDPs.

Molecular characterisation of the potato cyst nematode, Globodera ellingtonae Handoo et al., 2012 (Tylenchida: Heteroderidae) from Bolivia

Summary In 2022, during potato cyst nematode surveys in several departments of Bolivia, the potato cyst nematode Globodera ellingtonae Handoo et al., 2012 was detected for the first time in Bolivia from potato fields located in the Department of Tarija. Cysts and second-stage juveniles of the Bolivian populations were morphologically and morphometrically similar to those of the USA (Oregon and Idaho) and Argentina. Nine new ITS rRNA, 15 COI, 13 cytb gene sequences of G. ellingtonae from ten Bolivian populations were obtained in this study. The phylogenetic analyses of the ITS rRNA and cytb genes showed that all Bolivian sequences clustered together and formed a major clade with other G. ellingtonae sequences from Argentina, Chile and the USA. Molecular results confirmed the hypothesis that the mountain region in southern Bolivia, northwest Argentina and northern Chile could be considered as an ancient centre of origin of G. ellingtonae. The present molecular results suggested that G. ellingtonae was likely introduced into the USA from Chile, rather than from Bolivia and Argentina.

Population genetic structure of Dermatophagoides farinae (Acari: Pyroglyphidae) from different geographic populations based on mitochondrial cytochrome b gene

Dermatophagoides farinae (Acari: Pyroglyphidae) has been regarded as an important source of indoor allergens due to its prevalence and close proximity to humans. This study serves as an introductory probe into the population genetic structure of D. farinae, for which a few related studies of population genetic structure are available. In this study, the genetic structure of seven D. farinae populations in China was investigated based on the 372 bp mitochondrial cytochrome b (cytb) gene. A total of 29 haplotypes of the 68 cytb gene sequences were detected. The network diagram could be divided into Branch I (regions north of the Yangtze River: SJZ, CZ, FY and HN) and Branch II (regions south of the Yangtze River: AQ, WX and WH). Analysis of molecular variance (AMOVA) conducted according to the results of the network diagram revealed major genetic differentiation among groups, indicating that a significant genetic structure existed among groups. The results of population genetic structure could be largely attributed to geographical isolation. The existence of gene flow in a few populations may be influenced by grain trade activities. This study may facilitate a better understanding of the population genetic structure of D. farinae and could be utilized as the foundation for the development of efficient management strategies for D. farinae.

Genetic Diversity and Phylogeography of Taenioides cirratus in Five Geographical Populations Based on Mitochondrial COI and Cytb Gene Sequences

The genetic diversity of Taenioides cirratus was investigated on the basis of mitochondrial cytochrome c oxidase subunit I (COI) and cytochrome b (Cytb) gene sequences. A total of 159 specimens collected from the Chaohu Lake (CL), Nansihu Lake (NL), Taihu Lake (TL), Pearl River (PR), and Nandu River (NR) were sequenced. The total length of the sequence was 2485 bp with 412 polymorphic sites. A total of 73 haplotypes were identified, with Hap1 being the most widely distributed. The PR and NR populations showed high genetic diversity, while the CL population showed low genetic diversity. TL and NL showed high haplotype diversity but low nucleotide diversity. The analysis of molecular variance demonstrated that the sequence variations were mainly occurred among populations. T. cirratus populations are declining, and rare alleles are present at low frequencies, as analysed using a neutral test and a mismatched distribution analysis. There was a relatively high level of genetic differentiation among the populations of the Yangtze River basin (including NL), PR, and NR (Fst &gt; 0.15). The two similar phylogenetic trees constructed by the maximum likelihood (ML) and Bayesian inference (BI) methods presented three major lineages, of which lineage II contains haplotypes from PR and NR, lineage III contains haplotypes from CL, NL, TL, and PR, whereas lineage I contains only a portion of haplotypes from NR. Based on the neutral test, mismatch analysis, and Bayesian Skyline Plot (BSP), geological and climatic events were inferred to have played an important role in the historical dynamics of T. cirratus population. Hap1, Hap25, and Hap58 were inferred as possible ancestral haplotypes by network analysis. Our study offers an essential foundation for resource preservation and additional taxonomic clarification of T. cirratus.

Bioinformatic Analysis of the Cytb Gene to Estimate the Domestication Potential of Mahseer Fish Genus Tor spp.

The purpose of this study is to estimate the population of fish of the genus Tor as a source of proper domestication. Bioinformatic analysis was carried out by extracting data from the NCBI gene bank and alignment of the Cytb gene sequences using the ClustalW program to further analyze the nucleotide characteristics using MEGA software, determining genetic variation using the dnasp5 software, and using median joining analysis to reveal the relationship between Tor fish haplotypes from different locations. The results obtained from this study show that the haplotype diversity values of fish of the genus Tor spp. in the domesticated farm population and the natural population are still high, namely 0.9 and 0.8, respectively, as well as the occurrence of haplotype groupings based on other population origins. Domestication of Tor genus fish can be sourced from domesticated farms, and the wild is still ideal because the haplotype diversity is still high.

Genetic characterization of fish, Cynoglossus lingua Hamilton, 1822 found in Chandipur Coast of Odisha using COI and Cytochrome b genes

Aim: In this study we characterized the fish Cynoglossus lingua collected from Chandipur Coast of Odisha using the mtDNA markers cytochrome c oxidase I (COI) and cytochrome b (Cyt-b) genes. Methodology: Samples of flat fish were collected from Balaramgadi fish landing center, Chandipur sea beach, Odisha coast. The collected specimens were initially identified based on the morphology using standard taxonomic keys. Molecular characterization was accomplished through PCR amplification of COI and Cyt-b genes. The sequences were subjected to BLAST analysis and aligned using Clustal W for homology mapping. Phylogenetic status was inferred by the neighbor-joining method using MEGA v7.0. Results: The taxonomic keys and the COI and Cyt-b gene sequences identified the specimens as C. lingua. The COI and Cyt-b gene sequences showed 98% and 99% sequence similarity with C. lingua isolate FMU.CIFRI.FF-7 COI gene (GenBank accession: MN889532.1) and the C. lingua isolate FM.CIFRI.CYTB.FF-7 Cyt-b gene (GenBank accession MT362541.1), respectively. Interpretation: The study supports the reliability of combining classical taxonomic approaches with molecular analysis using COI and Cyt-b genes for the identification of C. lingua. Key words: Cynoglossus lingua, COI gene, Cytochrome b gene, DNA barcoding, Molecular taxonomy

Phylogenics of the genus Glossogobius in the Mekong Delta based on the mitochondrial cytochrome b (cytb) gene

The Glossogobius species play an essential role in food supply and are distributed widely from the marine to freshwater, especially in the Mekong Delta, Vietnam (VMD). Some of their morphometrics and meristics are observed to vary with species and sampling sites. Therefore, the present study aims to verify if their mitochondrial Cytochrome b (Cytb) gene, one of the popular gene sequences used in fish phylogenic variation assessment, varies with species and sampling sites in the VMD. The Cytb gene size was 1300 bp for GcytbH/GcytbL primer pair and 1045 bp for GluMuq1-F/Mixcyto937-2R. The genetic distances within and among these three fish species groups were 0–11%. The Cytb gene sequences’ similarity between this study and the NCBI database was 85.84–100%. The Glossogobius specimens were observed to disperse in small branches of the phylogenetic tree with a low K2P value, suggesting that the Cytb genetic diversity may be low among species.

First record of the flat-skulled woolly bat Kerivouladepressa and the Indochinese woolly bat K.dongduongana (Chiroptera, Vespertilionidae) in China.

Recent studies have revealed that the Kerivouladepressa complex should be divided into two species, K.depressa distributed mainly in Myanmar, Vietnam, Laos and Cambodia, and K.dongduongana found only in the Annamite Mountains of Vietnam, Laos and Cambodia. In November 2018 and April 2019, 24 woolly bats were collected by two-band harp traps in Xishuangbanna, Yunnan, China. Based on morphological, morphometric, and phylogenetic (COI, Cytb, and RAG2 gene sequences) analyses, these bats were identified as K.depressa and K.dongduongana, representing two new species records for the country. Including the new records, six Kerivoula species have been recorded in China, namely K.depressa, K.dongduongana, K.furva, K.kachinensis, K.picta and K.titania. To facilitate their identification and biological research in the future, we have provided an up-to-date key to all Kerivoula species occurring in China.